Strain Name: |
B6.Cg-Tg(Prrx1-cre)1Cjt/J |
|---|---|
Stock Number: |
005584 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | Prrx1; cre; Tg(Prrx1-cre)1Cjt; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Hemizygote x +/+ sibling (Female x Male) Species laboratory mouse Donating Investigator Clifford Tabin, Harvard Medical School Generation ?+N12+2F4 (24-APR-08) Strain Description
Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the paired related homeobox 1 promoter. Cre recombinase expression closely patterns endogenous gene expression and is detectable by embryonic day 9.5. Some recombination occurs in the female germline. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in early limb bud mesenchyme. This strain represents an effective tool for generating tissue specific-targeted mutants useful in studies of limb bud development and patterning.Strain Development
A transgenic construct containing cre coding sequence under the control of the paired related homeobox 1 promoter was microinjected into B6SJLF2 donor oocytes. Founder animals were backcrossed to C57BL/6J for 12 generations.
Gene & Allele Details
| Allele Symbol | Tg(Prrx1-cre)1Cjt | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Clifford J Tabin | ||
| Common Name(s) | Prx-1 Cre; Prx-cre; Prx1-cre; Prx1cre; Prx1Cre; | ||
| Mutation Made By | Malcolm Logan, National Institute for Medical Research | ||
| Strain of Origin | (C57BL/6J x SJL/J)F2 | ||
| Site of Expression | early limb bud mesenchyme and in a subset of craniofacial mesenchyme, some female germline expression | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Prrx1, paired related homeobox 1, rat | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of a Prrx1 derived enhancer. This transgene is expressed in the early limb bud mesenchyme and in a subset of craniofacial mesenchyme. A germline Cre recombinase activity was observed in female, but not in male, as partially penetrant trait depending on the particular gene flanked by loxP sites. [MGI Ref ID J:77872] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Generic Cre
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as homozygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (115 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox System (Cre Recombinase Expression)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesLogan M; Martin JF; Nagy A; Lobe C; Olson EN; Tabin CJ. 2002. Expression of Cre recombinase in the developing mouse limb bud driven by a Prxl enhancer. Genesis 33(2):77-80. [PubMed: 12112875] [MGI Ref ID J:77872]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Prrx1-cre)1Cjt/J |
| Stock Number: | 005584 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $236.40 | Hemizygous for Tg(Prrx1-cre)1Cjt | |||
| Pair | $288.65 | Hemizygous for Tg(Prrx1-cre)1Cjt x Noncarrier for Tg(Prrx1-cre)1Cjt | |||
| Pair | $288.65 | Noncarrier for Tg(Prrx1-cre)1Cjt x Hemizygous for Tg(Prrx1-cre)1Cjt | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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