Strain Name:

B6.129S-Atxn1tm1Hzo/J

Stock Number:

005601

Order this mouse

Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating Investigator Huda Zoghbi,   Baylor College of Medicine

Description
Mice that are heterozygous for the targeted mutation are viable but have a reduced lifespan (34-40 weeks). The allele consists of a 154 CAG trinucleotide repeat unit placed within exon 8 of the targeted endogenous mouse locus. Modified transcripts and protein can be detected in brain tissue. By 8 weeks of age, mutant mice exhibit noticeable growth retardation. Progressive neurological degeneration initiates by 9 weeks of age, when mutant mice begin to exhibit a clasping phenotype when held by the tail. By 20 weeks of age muscle wasting, ataxia and an abnormal gait are observed. A lack of motor coordination is detected via an accelerating rotarod test by 5 to 7 weeks. Cognitive defects include poor spatial learning performance and reduced Pavlovian conditioned fear response (impaired memory). Hippocampal basal synaptic function is impaired. Immunohistochemical and immunofluorescent analysis of brain tissue reveals neuronal intranuclear inclusions by 6 weeks of age. Older animals exhibit decreased brain weight, reduced dendritic arborization and loss of Purkinje cells. The onset and progression of the phenotype observed in these mutant mice mimics many of the features of spinaocerebellar ataxia type 1 (SCA1) and my be useful in SCA1- and neurodegenerative disease- related studies.

Development
A targeting vector containing sequence of an expanded repeat of 154 CAGs and a floxed (loxP site flanked) neomycin resistance selection cassette was used to disrupt exon 8. The construct was electroporated into 129S7/SvEvBrd-Hprtb-m2 derived AB2.2 embryonic stem (ES) cells which were transiently transfected with a Cre recombinase vector to remove the selection cassette. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then backcrossed to the same for 10 generations.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).
Spinocerebellar Ataxia 1; SCA1
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Atxn1tm1Hzo/Atxn1+

        B6.129S7-Atxn1tm1Hzo
  • mortality/aging
  • premature death
    • mice die before 60 weeks of age   (MGI Ref ID J:119643)
    • mice exhibit premature lethality   (MGI Ref ID J:177841)
    • however, exercise improves survival   (MGI Ref ID J:177841)
  • nervous system phenotype
  • decreased Purkinje cell number   (MGI Ref ID J:119643)
  • neuronal intranuclear inclusions   (MGI Ref ID J:177841)
  • behavior/neurological phenotype
  • abnormal associative learning
    • impaired fear conditioning   (MGI Ref ID J:177841)
  • ataxia   (MGI Ref ID J:119643)
  • hypoactivity
    • in an open field test   (MGI Ref ID J:177841)
  • impaired coordination   (MGI Ref ID J:119643)
    • on a rotarod and dowel test   (MGI Ref ID J:177841)
    • mice exhibit increased latency to cross a dowel rod and walk off the rod fewer times than wild-type mice   (MGI Ref ID J:198661)
  • growth/size/body phenotype
  • decreased body weight   (MGI Ref ID J:177841)

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Atxn1tm1Hzo/Atxn1+

        involves: 129S7/SvEvBrd * C57BL/6
  • mortality/aging
  • premature death
    • premature death first occurs between 35 to 45 weeks of age   (MGI Ref ID J:77225)
    • none of the mice survive past 50 weeks of age   (MGI Ref ID J:77225)
  • growth/size/body phenotype
  • cachexia
    • mice start losing weight after 20 weeks of age   (MGI Ref ID J:77225)
  • slow postnatal weight gain
    • growth retardation starts at 8 weeks of age   (MGI Ref ID J:77225)
    • mice weigh 20% less than wild-type littermates by 11 weeks of age   (MGI Ref ID J:77225)
  • behavior/neurological phenotype
  • abnormal cued conditioning behavior
    • in a context fear test, mice display significantly less freezing to the conditioned stimulus 24 hours after the training period but not 1 hour after   (MGI Ref ID J:77225)
  • abnormal gait
    • evident by 20 weeks of age   (MGI Ref ID J:77225)
  • abnormal spatial learning
    • 7-8 week old mice take more time and swim further to locate a submerged but visible platform in a morris water test during the first 6 trials   (MGI Ref ID J:77225)
    • mice perform as wells as wild-type controls in later trials   (MGI Ref ID J:77225)
    • 7-8 week old mice take more time and swim further to locate a hidden platform in a morris water test regardless of trial number   (MGI Ref ID J:77225)
  • ataxia
    • evident by 20 weeks of age   (MGI Ref ID J:77225)
  • impaired coordination
    • retention time in a rotarod test is impaired by about half for both 5 and 7 week old mice   (MGI Ref ID J:77225)
  • limb grasping
    • mice have a clasping phenotype when lifted by the tail starting at 9 weeks of age   (MGI Ref ID J:77225)
  • nervous system phenotype
  • abnormal Purkinje cell dendrite morphology
    • there is a reduction in dendritic arbor of cerebellar Purkinje neurons from mice 6 to 11 weeks of age   (MGI Ref ID J:77225)
    • this reduction in dendritic arbor leads to a reduction in membrane capacitance   (MGI Ref ID J:77225)
  • abnormal excitatory postsynaptic potential
    • EPSP magnitude is significantly decreased in the hippocampus 90 minutes after high-frequency stimulation   (MGI Ref ID J:77225)
  • decreased Purkinje cell number
    • significantly fewer Purkinje cells are present in 40-week old mice compared to wild-type littermates   (MGI Ref ID J:77225)
  • decreased brain weight
    • brain weight is significantly reduced by 16 weeks of age   (MGI Ref ID J:77225)
  • dilated brain ventricles
    • all ventricles are dilated by 40 weeks of age   (MGI Ref ID J:77225)
  • neuronal intranuclear inclusions
    • ubiquitinated neuronal intranuclear inclusions (NI) are present in CA1 hippocampal neurons by 7 weeks of age   (MGI Ref ID J:77225)
    • NI are also present in cortical neurons and thalamic nuclei by 7 weeks of age   (MGI Ref ID J:77225)
    • NI are present in numerous parts of the brain during the endstage of disease   (MGI Ref ID J:77225)
  • reduced long term potentiation
    • hippocampus LTP is significantly reduced in 24 week old mice   (MGI Ref ID J:77225)
  • muscle phenotype
  • muscle degeneration
    • muscle wasting is evident by 20 weeks of age   (MGI Ref ID J:77225)
    • atrophy of lower limb muscles occurs by 30 weeks of age   (MGI Ref ID J:77225)
  • skeleton phenotype
  • kyphosis
    • is observed by 30 weeks of age   (MGI Ref ID J:77225)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Growth Defects

Neurobiology Research
Ataxia (Movement) Defects
Behavioral and Learning Defects
Cerebellar Defects
      Purkinje cell defect

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Atxn1tm1Hzo
Allele Name targeted mutation 1, Huda Y Zoghbi
Allele Type Targeted (knock-in)
Common Name(s) Atx1-KI; Sca1-KI; Sca1154Q/2Q; Sca1154Q; atxn1154Q;
Strain of Origin129S7/SvEvBrd-Hprt
ES Cell Line NameAB2.2
ES Cell Line Strain129S7/SvEvBrd-Hprt
Promoter Atxn1, ataxin 1, mouse, laboratory
Molecular Note An expanded tract of 154 CAG repeats was engineered and knocked into exon 8. Additionally, a floxed neo-TK cassette was inserted into the upstream intron and subsequently deleted in ES cells via cre mediated recombination. While equivalent transcript levels were identified in both mutant and wild-type mice by RT-PCR, reduced levels of mutant protein were observed in brain tissue. The authors attributed this apparent reduction in protein level to an increased difficulty of extraction or solubilization dueto the mutant protein accumulating into nuclear aggregates as mice age. [MGI Ref ID J:77225]

Genotyping

Genotyping Information

Genotyping Protocols

Atxn1tm1Hzo repeat assay, Standard PCR
Atxn1tm1Hzo, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Watase K; Weeber EJ; Xu B; Antalffy B; Yuva-Paylor L; Hashimoto K; Kano M; Atkinson R; Sun Y; Armstrong DL; Sweatt JD; Orr HT; Paylor R; Zoghbi HY. 2002. A long CAG repeat in the mouse Sca1 locus replicates SCA1 features and reveals the impact of protein solubility on selective neurodegeneration. Neuron 34(6):905-19. [PubMed: 12086639]  [MGI Ref ID J:77225]

Additional References

Atxn1tm1Hzo related

Bowman AB; Lam YC; Jafar-Nejad P; Chen HK; Richman R; Samaco RC; Fryer JD; Kahle JJ; Orr HT; Zoghbi HY. 2007. Duplication of Atxn1l suppresses SCA1 neuropathology by decreasing incorporation of polyglutamine-expanded ataxin-1 into native complexes. Nat Genet 39(3):373-379. [PubMed: 17322884]  [MGI Ref ID J:119643]

Chen KA; Cruz PE; Lanuto DJ; Flotte TR; Borchelt DR; Srivastava A; Zhang J; Steindler DA; Zheng T. 2011. Cellular fusion for gene delivery to SCA1 affected Purkinje neurons. Mol Cell Neurosci 47(1):61-70. [PubMed: 21420496]  [MGI Ref ID J:177962]

Crespo-Barreto J; Fryer JD; Shaw CA; Orr HT; Zoghbi HY. 2010. Partial loss of ataxin-1 function contributes to transcriptional dysregulation in spinocerebellar ataxia type 1 pathogenesis. PLoS Genet 6(7):e1001021. [PubMed: 20628574]  [MGI Ref ID J:162235]

Cvetanovic M; Kular RK; Opal P. 2012. LANP mediates neuritic pathology in Spinocerebellar ataxia type 1. Neurobiol Dis 48(3):526-32. [PubMed: 22884877]  [MGI Ref ID J:197504]

Cvetanovic M; Patel JM; Marti HH; Kini AR; Opal P. 2011. Vascular endothelial growth factor ameliorates the ataxic phenotype in a mouse model of spinocerebellar ataxia type 1. Nat Med 17(11):1445-7. [PubMed: 22001907]  [MGI Ref ID J:178113]

Dion V; Lin Y; Hubert L Jr; Waterland RA; Wilson JH. 2008. Dnmt1 deficiency promotes CAG repeat expansion in the mouse germline. Hum Mol Genet 17(9):1306-17. [PubMed: 18252747]  [MGI Ref ID J:133928]

Fryer JD; Yu P; Kang H; Mandel-Brehm C; Carter AN; Crespo-Barreto J; Gao Y; Flora A; Shaw C; Orr HT; Zoghbi HY. 2011. Exercise and genetic rescue of SCA1 via the transcriptional repressor Capicua. Science 334(6056):690-3. [PubMed: 22053053]  [MGI Ref ID J:177841]

Gatchel JR; Watase K; Thaller C; Carson JP; Jafar-Nejad P; Shaw C; Zu T; Orr HT; Zoghbi HY. 2008. The insulin-like growth factor pathway is altered in spinocerebellar ataxia type 1 and type 7. Proc Natl Acad Sci U S A 105(4):1291-6. [PubMed: 18216249]  [MGI Ref ID J:131821]

Hubert L Jr; Lin Y; Dion V; Wilson JH. 2011. Xpa deficiency reduces CAG trinucleotide repeat instability in neuronal tissues in a mouse model of SCA1. Hum Mol Genet :. [PubMed: 21926083]  [MGI Ref ID J:177762]

Jafar-Nejad P; Ward CS; Richman R; Orr HT; Zoghbi HY. 2011. Regional rescue of spinocerebellar ataxia type 1 phenotypes by 14-3-3epsilon haploinsufficiency in mice underscores complex pathogenicity in neurodegeneration. Proc Natl Acad Sci U S A 108(5):2142-7. [PubMed: 21245341]  [MGI Ref ID J:169114]

Jorgensen ND; Andresen JM; Lagalwar S; Armstrong B; Stevens S; Byam CE; Duvick LA; Lai S; Jafar-Nejad P; Zoghbi HY; Clark HB; Orr HT. 2009. Phosphorylation of ATXN1 at Ser776 in the cerebellum. J Neurochem 110(2):675-86. [PubMed: 19500214]  [MGI Ref ID J:150968]

Ju H; Kokubu H; Todd TW; Kahle JJ; Kim S; Richman R; Chirala K; Orr HT; Zoghbi HY; Lim J. 2013. Polyglutamine disease toxicity is regulated by Nemo-like kinase in spinocerebellar ataxia type 1. J Neurosci 33(22):9328-36. [PubMed: 23719801]  [MGI Ref ID J:198661]

Lee Y; Fryer JD; Kang H; Crespo-Barreto J; Bowman AB; Gao Y; Kahle JJ; Hong JS; Kheradmand F; Orr HT; Finegold MJ; Zoghbi HY. 2011. ATXN1 protein family and CIC regulate extracellular matrix remodeling and lung alveolarization. Dev Cell 21(4):746-57. [PubMed: 22014525]  [MGI Ref ID J:178303]

Park J; Al-Ramahi I; Tan Q; Mollema N; Diaz-Garcia JR; Gallego-Flores T; Lu HC; Lagalwar S; Duvick L; Kang H; Lee Y; Jafar-Nejad P; Sayegh LS; Richman R; Liu X; Gao Y; Shaw CA; Arthur JS; Orr HT; Westbrook TF; Botas J; Zoghbi HY. 2013. RAS-MAPK-MSK1 pathway modulates ataxin 1 protein levels and toxicity in SCA1. Nature 498(7454):325-31. [PubMed: 23719381]  [MGI Ref ID J:198731]

Perroud B; Jafar-Nejad P; Wikoff WR; Gatchel JR; Wang L; Barupal DK; Crespo-Barreto J; Fiehn O; Zoghbi HY; Kaddurah-Daouk R. 2013. Pharmacometabolomic signature of ataxia SCA1 mouse model and lithium effects. PLoS One 8(8):e70610. [PubMed: 23936457]  [MGI Ref ID J:205774]

Shiwaku H; Yoshimura N; Tamura T; Sone M; Ogishima S; Watase K; Tagawa K; Okazawa H. 2010. Suppression of the novel ER protein Maxer by mutant ataxin-1 in Bergman glia contributes to non-cell-autonomous toxicity. EMBO J 29(14):2446-60. [PubMed: 20531390]  [MGI Ref ID J:162095]

Takechi Y; Mieda T; Iizuka A; Toya S; Suto N; Takagishi K; Nakazato Y; Nakamura K; Hirai H. 2013. Impairment of spinal motor neurons in spinocerebellar ataxia type 1-knock-in mice. Neurosci Lett 535:67-72. [PubMed: 23328439]  [MGI Ref ID J:197752]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as heterozygotes. The Donating Investigator indicates that it is best to use male carrier mice in breeding strategies because the allele appears less stable in female mutants. Additionally, the phenotype of homozygous mice is too severe for successful breeding.
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.5)