Strain Name: |
B6.129P-Psen2tm1Bdes/J |
|---|---|
Stock Number: |
005617 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Psen2; Psen2tm1Bdes; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Bart de Strooper, KU Leuven and VIB Strain Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA or protein) is detected by Northern or Western blot analysis. Alveolar wall thickening, fibrotic deposits, hemorahages in alveoli and airways are observed by histological analysis of lung tissue from homozygotes 3 months of age and older. This mutant mouse strain may be useful in studies of Alzheimer's disease.Strain Development
A targeting vector containing hygromycin resistance gene driven by the phosphoglycerate kinase promoter was used to disrupt exon 5 and introducing a frame shift between exons 4 and 6. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were crossed to C57BL/6J mice, and then backcrossed to C57BL/6J for 14 generations.
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Psen2tm1Bdes | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Bart de Strooper | ||
| Common Name(s) | PS2-; | ||
| Mutation Made By | Bart de Strooper, KU Leuven and VIB | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Psen2, presenilin 2 | ||
| Chromosome | 1 | ||
| Gene Common Name(s) | AD3L; AD4; AI266870; ALG-3; Ad4h; PS-2; PS2; STM2; alzheimer disease 4 homolog; expressed sequence AI266870; | ||
| Molecular Note | Replacement of exon 5 by a hygromycin cassette resulted in a frame shift between exons 4 and 6. Northern blots of RNA from brain, kidney, lung, heart, and liver of homozygous mutant mice confirmed the null mutation of the Psen2 gene. Western blots of the same tissues from homozygous mutant mice using antibodies specific for both the N- and C-termini of the Psen2 protein confirmed the mutation. [MGI Ref ID J:58118] | ||
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Psen2tm1Bdes
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as homozygotes. |
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Congenic Nomenclature
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Psen2tm1Bdes relatedCardiovascular Research
Vascular Defects
Mouse/Human Gene Homologs
Alzheimer's
Neurobiology Research
Alzheimer's Disease (Presenilin mutants)
Alzheimer's Disease
Neurobiology Research
Alzheimer's Disease
Selected Reference(s)
Additional ReferencesHerreman A; Hartmann D; Annaert W; Saftig P; Craessaerts K; Serneels L; Umans L; Schrijvers V; Checler F; Vanderstichele H; Baekelandt V; Dressel R; Cupers P; Huylebroeck D; Zwijsen A; Van Leuven F; De Strooper B. 1999. Presenilin 2 deficiency causes a mild pulmonary phenotype and no changes in amyloid precursor protein processing but enhances the embryonic lethal phenotype of presenilin 1 deficiency. Proc Natl Acad Sci U S A 96(21):11872-7. [PubMed: 10518543] [MGI Ref ID J:58118]
| Strain Name: | B6.129P-Psen2tm1Bdes/J |
| Stock Number: | 005617 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
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- Use of MICE by companies or for-profit entities requires a license prior to shipping.
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