Strain Name: |
B6;129P2-Bace2tm1Bdes/J |
|---|---|
Stock Number: |
005618 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Bace2; Bace2tm1Bdes; |
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Bart de Strooper, KU Leuven and VIB Strain Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Transcripts derived from the targeted locus do not contain exon 6. One of the enzyme's active sites is encoded by exon 6 so consequently, proteins generated from the targeted locus lack beta-secretase activity. This mutant mouse strain may be useful in studies of Alzheimer's disease.Strain Development
A targeting vector containing a loxP site and hygromycin resistance gene flanked by FRT sites and was introduced into intron 5. A second loxP site was inserted within intron 6. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to mice expressing Cre recombinase under the control of the phosphoglycerate kinase promoter to delete exon 6. Heterozygotes were bred to generate homozygotes.
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Bace2tm1Bdes | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Bart De Strooper | ||
| Common Name(s) | BACE2delta6; Bace2tm1Psa; | ||
| Mutation Made By | Bart de Strooper, KU Leuven and VIB | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Strain | 129 | ||
| Gene Symbol and Name | Bace2, beta-site APP-cleaving enzyme 2 | ||
| Chromosome | 16 | ||
| Gene Common Name(s) | 1110059C24Rik; AEPLC; AI850424; ALP56; ARP1; ASP1; ASP21; BAE2; CDA13; CEAP1; DRAP; RIKEN cDNA 1110059C24 gene; expressed sequence AI850424; | ||
| Molecular Note | Cre mediated recombination was used to remove exon 6 from the locus. [MGI Ref ID J:100925] | ||
| Control | ||
|---|---|---|
| 100492 B6129PF1/J | ||
| Considerations for Choosing Controls | ||
Bace2tm1Bdes
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as homozygotes. |
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Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Mouse/Human Gene Homologs
Alzheimer's
Neurobiology Research
Alzheimer's Disease (Bace mutants)
Alzheimer's Disease
Selected Reference(s)
Additional ReferencesDominguez D; Tournoy J; Hartmann D; Huth T; Cryns K; Deforce S; Serneels L; Camacho IE; Marjaux E; Craessaerts K; Roebroek AJ; Schwake M; D'Hooge R; Bach P; Kalinke U; Moechars D; Alzheimer C; Reiss K; Saftig P; De Strooper B. 2005. Phenotypic and biochemical analyses of BACE1- and BACE2-deficient mice. J Biol Chem 280(35):30797-806. [PubMed: 15987683] [MGI Ref ID J:100925]
| Strain Name: | B6;129P2-Bace2tm1Bdes/J |
| Stock Number: | 005618 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
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