Strain Name: |
B6;D2-Tg(S100B-EYFP)1Wjt/J |
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Stock Number: |
005620 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | S100B; YFP; Tg(S100B-EYFP)1Wjt; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Wesley Thompson, University of Texas Strain Description
Mice homozygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Yellow Fluorescent Protein (EYFP) under the direction of the human S100 calcium-binding protein, beta (neural) promoter. Fluorescence is detected in Schwann cells, motor axons, microglia, a subset of Bergmann glia and some muscle macrophages. Strong fluorescent signal is detected in the Schwann cells of sternomastoid, extensor digitorum longus, triangularis sterni and diaphragm muscles and in the motor axons of the triangularis sterni. EYFP expression is detected at birth in glial cells and at postnatal day 7 in motor axons. This mutant mouse strain may be useful in studies of vital imaging of neuronal and glial cells.Strain Development
A transgenic construct containing an Enhanced Yellow Fluorescent Protein gene under the control of the human S100 calcium-binding protein, beta (neural) promoter, was injected into B6D2F2 donor eggs. Founder line YA was subsequently established and bred to B6D2F2 mice.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(S100B-EYFP)1Wjt | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Wesley Thompson | ||
| Mutation Made By | Wesley Thompson, University of Texas | ||
| Strain of Origin | (C57BL/6 x DBA)F2 | ||
| Site of Expression | Schwann cells, motor axons, microglia, a subset of Bergmann glia and some muscle macrophages. Strong fluorescent signal is detected in the Schwann cells of sternomastoid, extensor digitorum longus, triangularis sterni and diaphragm muscles and in the motor axons of the triangularis sterni. EYFP expression is detected at birth in glial cells and at postnatal day 7 in motor axons. | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Promoter | S100B, S100 calcium binding protein B, human | ||
| Molecular Note | The transgene contains an Enhanced Yellow Fluorescent Protein gene under the control of the human S100B calcium-binding protein, beta (neural) promoter. [J:94808] | ||
Control Information
| Control | ||
|---|---|---|
| 100006 B6D2F1/J | ||
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as homozygotes. |
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Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (135 strains)
005621 B6;D2-Tg(S100B-EGFP)1Wjt/J 003198 STOCK Tg(huS100B)5Daoh/J
005483 129-Tg(CAG-EYFP)7AC5Nagy/J 006148 B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 007612 B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J 007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J 005630 B6.Cg-Tg(Thy1-EYFP)15Jrs/J 003709 B6.Cg-Tg(Thy1-YFP)16Jrs/J 005627 B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J 003782 B6.Cg-Tg(Thy1-YFPH)2Jrs/J 007606 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J 007610 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 007880 B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J 006618 C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J 006362 C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J 005130 STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Research Applications
This mouse can be used to support research in many areas including:
YFP relatedNeurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Zuo Y; Lubischer JL; Kang H; Tian L; Mikesh M; Marks A; Scofield VL; Maika S; Newman C; Krieg P; Thompson WJ. 2004. Fluorescent proteins expressed in mouse transgenic lines mark subsets of glia, neurons, macrophages, and dendritic cells for vital examination. J Neurosci 24(49):10999-1009. [PubMed: 15590915] [J:94808]
Price and Supply Information
| Strain Name: | B6;D2-Tg(S100B-EYFP)1Wjt/J |
| Stock Number: | 005620 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
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| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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