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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.129S-Shhtm1(EGFP/cre)Cjt/J (Changed: 05-SEP-06 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System +/+ sibling x Heterozygote (Female x Male) 29-DEC-06 Species laboratory mouse Generation N6F7 (14-JAN-09) Donating Investigator Clifford Tabin, Harvard Medical School Description
This strain expresses a fusion product involving Enhanced Green Fluorescent Protein (EGFP) and Cre recombinase from the endogenous Shh locus. EGFP and cre expression are consistent with the endogenous gene. Fluorescence is detected in the distal posterior region of the limb buds of embryos aged embryonic day 10 to 12 and colocalizes with the endogenous gene product (mRNA). The donating investigator reports that it is not uncommon for a mosaic expression pattern to be exhibited when the allele is inherited through the female germline. It is recommended that this allele be passed through the male germline when conducting experiments involving cre-induced recombination. Mice homozygous for the mutation develop a limited limb skeleton and lack digit 2. Homozygous mice are not viable or fertile. Heterozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of limb patterning and development.Development
A targeting vector containing a fusion product involving Enhanced Green Fluorescent Protein (EGFP) and Cre recombinase was inserted at the ATG of Shh and disrupted the sequence encoding the first 12 amino acids. The construct was electroporated into 129S6/SvEv-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for at least 5 generations.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
004293 129-Shhtm2Amc/J 000214 B10.D2/nSn-ShhHx/J 005623 B6.129S-Shhtm2(cre/ESR1)Cjt/J 008466 B6.129X1-Shhtm6Amc/J 003318 STOCK Shhtm1Amc/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Shhtm1(EGFP/cre)Cjt/Shhtm1(EGFP/cre)Cjt
Background Not Specified
- limbs/digits/tail phenotype
- abnormal skeleton extremities morphology (MGI Ref ID J:92504)
- a limited skeletal structure consisting of the humerus, radius and single digit 1 is seen
- skeleton phenotype
- abnormal skeleton extremities morphology (MGI Ref ID J:92504)
- a limited skeletal structure consisting of the humerus, radius and single digit 1 is seen
This mouse can be used to support research in many areas including:
GFP relatedDevelopmental Biology Research
Embryonic Lethality (Homozygous)
Limb Patterning Defects
Skeletal Defects
Research Tools
Cre-lox System
Cre Recombinase Expression: Germline/Embryonic Expression
Developmental Biology Research
Cre-lox System
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
cre relatedResearch Tools
Fluorescent Proteins
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Shhtm1(EGFP/cre)Cjt | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Clifford J Tabin | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | ShhGFP-Cre; Shhcre/Gfp; Shhgfp-Cre; Shhgfpcre; | ||
| Mutation Made By | Clifford Tabin, Harvard Medical School | ||
| Site of Expression | Distal posterior region of the limb buds of embryos aged embryonic day 10 to 12; also a Cre strain. | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Gene Symbol and Name | Shh, sonic hedgehog | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | Dsh; HHG1; HLP3; HPE3; Hhg1; Hx; Hxl3; M100081; MCOPCB5; SMMCI; TPT; TPTPS; hedgehog gene 1; hemimelic extra toes; hemimelic extratoes like 3; short digits; | ||
| Driver Note | Shh | ||
| Molecular Note | A cassette containing an in frame fusion between GFP and cre was inserted at the ATG. Upon recombination, the first 35 base pairs after the ATG were deleted. Fluorescence was detected in the distal posterior region of E10-E12 embryos. [MGI Ref ID J:92504] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Shhtm1(EGFP/cre)Cjt, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Harfe BD; Scherz PJ; Nissim S; Tian H; McMahon AP; Tabin CJ. 2004. Evidence for an expansion-based temporal Shh gradient in specifying vertebrate digit identities. Cell 118(4):517-28. [PubMed: 15315763] [MGI Ref ID J:92504]
Additional References
Shhtm1(EGFP/cre)Cjt relatedChamberlain CE; Jeong J; Guo C; Allen BL; McMahon AP. 2008. Notochord-derived Shh concentrates in close association with the apically positioned basal body in neural target cells and forms a dynamic gradient during neural patterning. Development 135(6):1097-106. [PubMed: 18272593] [MGI Ref ID J:132152]
Glotzer DJ; Zelzer E; Olsen BR. 2008. Impaired skin and hair follicle development in Runx2 deficient mice. Dev Biol 315(2):459-73. [PubMed: 18262513] [MGI Ref ID J:132568]
Goss AM; Tian Y; Tsukiyama T; Cohen ED; Zhou D; Lu MM; Yamaguchi TP; Morrisey EE. 2009. Wnt2/2b and beta-catenin signaling are necessary and sufficient to specify lung progenitors in the foregut. Dev Cell 17(2):290-8. [PubMed: 19686689] [MGI Ref ID J:153098]
Gritli-Linde A; Hallberg K; Harfe BD; Reyahi A; Kannius-Janson M; Nilsson J; Cobourne MT; Sharpe PT; McMahon AP; Linde A. 2007. Abnormal hair development and apparent follicular transformation to mammary gland in the absence of hedgehog signaling. Dev Cell 12(1):99-112. [PubMed: 17199044] [MGI Ref ID J:117334]
Harfe BD; McManus MT; Mansfield JH; Hornstein E; Tabin CJ. 2005. The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb. Proc Natl Acad Sci U S A 102(31):10898-903. [PubMed: 16040801] [MGI Ref ID J:100475]
Harris KS; Zhang Z; McManus MT; Harfe BD; Sun X. 2006. Dicer function is essential for lung epithelium morphogenesis. Proc Natl Acad Sci U S A 103(7):2208-13. [PubMed: 16452165] [MGI Ref ID J:106072]
Joksimovic M; Yun BA; Kittappa R; Anderegg AM; Chang WW; Taketo MM; McKay RD; Awatramani RB. 2009. Wnt antagonism of Shh facilitates midbrain floor plate neurogenesis. Nat Neurosci 12(2):125-31. [PubMed: 19122665] [MGI Ref ID J:146200]
Kim BM; Mao J; Taketo MM; Shivdasani RA. 2007. Phases of canonical Wnt signaling during the development of mouse intestinal epithelium. Gastroenterology 133(2):529-38. [PubMed: 17681174] [MGI Ref ID J:128278]
Kim BM; Miletich I; Mao J; McMahon AP; Sharpe PA; Shivdasani RA. 2007. Independent functions and mechanisms for homeobox gene Barx1 in patterning mouse stomach and spleen. Development 134(20):3603-13. [PubMed: 17855428] [MGI Ref ID J:128378]
Kittappa R; Chang WW; Awatramani RB; McKay RD. 2007. The foxa2 gene controls the birth and spontaneous degeneration of dopamine neurons in old age. PLoS Biol 5(12):e325. [PubMed: 18076286] [MGI Ref ID J:130846]
Lin C; Yin Y; Long F; Ma L. 2008. Tissue-specific requirements of beta-catenin in external genitalia development. Development 135(16):2815-25. [PubMed: 18635608] [MGI Ref ID J:139251]
Munne PM; Tummers M; Jarvinen E; Thesleff I; Jernvall J. 2009. Tinkering with the inductive mesenchyme: Sostdc1 uncovers the role of dental mesenchyme in limiting tooth induction. Development 136(3):393-402. [PubMed: 19141669] [MGI Ref ID J:144193]
Rock JR; Cecilia Lopez M; Baker HV; Harfe BD. 2007. Identification of genes expressed in the mouse limb using a novel ZPA microarray approach. Gene Expr Patterns 8(1):19-26. [PubMed: 17911046] [MGI Ref ID J:127128]
Sanchez-Camacho C; Bovolenta P. 2008. Autonomous and non-autonomous Shh signalling mediate the in vivo growth and guidance of mouse retinal ganglion cell axons. Development 135(21):3531-41. [PubMed: 18832395] [MGI Ref ID J:143442]
Tang M; Miyamoto Y; Huang EJ. 2009. Multiple roles of {beta}-catenin in controlling the neurogenic niche for midbrain dopamine neurons. Development 136(12):2027-38. [PubMed: 19439492] [MGI Ref ID J:149534]
Tian H; Jeong J; Harfe BD; Tabin CJ; McMahon AP. 2005. Mouse Disp1 is required in sonic hedgehog-expressing cells for paracrine activity of the cholesterol-modified ligand. Development 132(1):133-42. [PubMed: 15576405] [MGI Ref ID J:94270]
Verheyden JM; Lewandoski M; Deng C; Harfe BD; Sun X. 2005. Conditional inactivation of Fgfr1 in mouse defines its role in limb bud establishment, outgrowth and digit patterning. Development 132(19):4235-45. [PubMed: 16120640] [MGI Ref ID J:101736]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as heterozygotes. Homozygous mice are not viable. Mating System +/+ sibling x Heterozygote (Female x Male) 29-DEC-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Heterozygous for Shhtm1(EGFP/cre)Cjt
Pairs /Price (US dollars $) Pair Genotype $297.85 Heterozygous for Shhtm1(EGFP/cre)Cjt x Wild-type for Shhtm1(EGFP/cre)Cjt $297.85 Wild-type for Shhtm1(EGFP/cre)Cjt x Heterozygous for Shhtm1(EGFP/cre)Cjt
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Heterozygous for Shhtm1(EGFP/cre)Cjt
Pairs /Price (US dollars $) Pair Genotype $387.30 Heterozygous for Shhtm1(EGFP/cre)Cjt x Wild-type for Shhtm1(EGFP/cre)Cjt $387.30 Wild-type for Shhtm1(EGFP/cre)Cjt x Heterozygous for Shhtm1(EGFP/cre)Cjt
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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