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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System +/+ sibling x Heterozygote (Female x Male) 29-AUG-06 Species laboratory mouse Generation N5+1F6 (04-NOV-09) Donating Investigator Clifford Tabin, Harvard Medical School Description
This strain expresses a fusion product involving Cre recombinase and a mutant form of the human estrogen receptor ligand binding domain from the endogenous Shh locus. The mutant human estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/ESR1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. Tamoxifen administration induces Cre recombinase expression in all cells that express the endogenous gene resulting in the deletion of the first 35 base pairs following the ATG. Homozygous mice are not viable or fertile. Heterozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of limb patterning and development.Development
A targeting vector containing a fusion product involving Cre recombinase and a mutant form of the human estrogen receptor ligand binding domain was inserted at the ATG of Shh. The construct was electroporated into 129S6/SvEv-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 5 generations.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Shh
004293 129-Shhtm2Amc/J 000214 B10.D2/nSn-ShhHx/J 008466 B6.129X1-Shhtm6Amc/J 005622 B6.Cg-Shhtm1(EGFP/cre)Cjt/J 003318 STOCK Shhtm1Amc/J View Strains carrying other alleles of Shh (5 strains)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Shhtm2(cre/ESR1)Cjt/Shh+
Background Not Specified
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:92504)
- mice are used for expression analysis only
This mouse can be used to support research in many areas including:
cre relatedDevelopmental Biology Research
Embryonic Lethality (Homozygous)
Limb Patterning Defects
Skeletal Defects
Research Tools
Cre-lox System
Cre Recombinase Expression: Inducible
Developmental Biology Research
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Shhtm2(cre/ESR1)Cjt | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Clifford J Tabin | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Shh-cre; ShhCreERT2; Shhtm2(cre-ERT2)Cjt; ShhCreER; ShhcreERT2; | ||
| Mutation Made By | Clifford Tabin, Harvard Medical School | ||
| Site of Expression | tamoxifen inducible Cre recombinase protein fused to a mutant form of the human estrogen receptor ligand binding domain; expression in all cells that express the Shh gene | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Shh, sonic hedgehog | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | Dsh; HHG1; HLP3; HPE3; Hhg1; Hx; Hxl3; M100081; MCOPCB5; SMMCI; TPT; TPTPS; hedgehog gene 1; hemimelic extra toes; hemimelic extratoes like 3; short digits; | ||
| Driver Note | Shh | ||
| Molecular Note | A cassette containing CreERT2 was inserted at the ATG. Upon recombination, the first 35 base pairs after the ATG were deleted. Cre was produced in all cells in which the endogenous mRNA was normally expressed. [MGI Ref ID J:92504] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Shhtm2(cre/ESR1)Cjt, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Harfe BD; Scherz PJ; Nissim S; Tian H; McMahon AP; Tabin CJ. 2004. Evidence for an expansion-based temporal Shh gradient in specifying vertebrate digit identities. Cell 118(4):517-28. [PubMed: 15315763] [MGI Ref ID J:92504]
Additional References
Shhtm2(cre/ESR1)Cjt relatedDevenport D; Fuchs E. 2008. Planar polarization in embryonic epidermis orchestrates global asymmetric morphogenesis of hair follicles. Nat Cell Biol 10(11):1257-68. [PubMed: 18849982] [MGI Ref ID J:145627]
Goddeeris MM; Rho S; Petiet A; Davenport CL; Johnson GA; Meyers EN; Klingensmith J. 2008. Intracardiac septation requires hedgehog-dependent cellular contributions from outside the heart. Development 135(10):1887-95. [PubMed: 18441277] [MGI Ref ID J:134643]
Greco V; Chen T; Rendl M; Schober M; Pasolli HA; Stokes N; Dela Cruz-Racelis J; Fuchs E. 2009. A two-step mechanism for stem cell activation during hair regeneration. Cell Stem Cell 4(2):155-69. [PubMed: 19200804] [MGI Ref ID J:149690]
Harris-Johnson KS; Domyan ET; Vezina CM; Sun X. 2009. beta-Catenin promotes respiratory progenitor identity in mouse foregut. Proc Natl Acad Sci U S A 106(38):16287-92. [PubMed: 19805295] [MGI Ref ID J:153236]
Jadhav AP; Cho SH; Cepko CL. 2006. Notch activity permits retinal cells to progress through multiple progenitor states and acquire a stem cell property. Proc Natl Acad Sci U S A 103(50):18998-9003. [PubMed: 17148603] [MGI Ref ID J:118372]
Lin C; Yin Y; Long F; Ma L. 2008. Tissue-specific requirements of beta-catenin in external genitalia development. Development 135(16):2815-25. [PubMed: 18635608] [MGI Ref ID J:139251]
McGlinn E; Richman JM; Metzis V; Town L; Butterfield NC; Wainwright BJ; Wicking C. 2008. Expression of the NET family member Zfp503 is regulated by hedgehog and BMP signaling in the limb. Dev Dyn 237(4):1172-82. [PubMed: 18351672] [MGI Ref ID J:132977]
Miyagawa S; Satoh Y; Haraguchi R; Suzuki K; Iguchi T; Taketo MM; Nakagata N; Matsumoto T; Takeyama KI; Kato S; Yamada G. 2009. Genetic interactions of the Androgen and Wnt/{beta}-catenin Pathways for the Masculinization of External Genitalia. Mol Endocrinol 23(6):871-880. [PubMed: 19282366] [MGI Ref ID J:148515]
Schuller U; Heine VM; Mao J; Kho AT; Dillon AK; Han YG; Huillard E; Sun T; Ligon AH; Qian Y; Ma Q; Alvarez-Buylla A; McMahon AP; Rowitch DH; Ligon KL. 2008. Acquisition of granule neuron precursor identity is a critical determinant of progenitor cell competence to form Shh-induced medulloblastoma. Cancer Cell 14(2):123-34. [PubMed: 18691547] [MGI Ref ID J:139574]
Thirumangalathu S; Harlow DE; Driskell AL; Krimm RF; Barlow LA. 2009. Fate mapping of mammalian embryonic taste bud progenitors. Development 136(9):1519-28. [PubMed: 19363153] [MGI Ref ID J:147959]
Tsao PN; Vasconcelos M; Izvolsky KI; Qian J; Lu J; Cardoso WV. 2009. Notch signaling controls the balance of ciliated and secretory cell fates in developing airways. Development 136(13):2297-307. [PubMed: 19502490] [MGI Ref ID J:150051]
Verheyden JM; Sun X. 2008. An Fgf/Gremlin inhibitory feedback loop triggers termination of limb bud outgrowth. Nature 454(7204):638-41. [PubMed: 18594511] [MGI Ref ID J:138578]
Zhang Z; Verheyden JM; Hassell JA; Sun X. 2009. FGF-regulated Etv genes are essential for repressing Shh expression in mouse limb buds. Dev Cell 16(4):607-13. [PubMed: 19386269] [MGI Ref ID J:149477]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number FGB29
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous mice are bred to wildtype siblings (or C57BL/6J). Homozygous mice are not viable. Mating System +/+ sibling x Heterozygote (Female x Male) 29-AUG-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $243.50 Female or Male Heterozygous for Shhtm2(cre/ESR1)Cjt
Pairs /Price (US dollars $) Pair Genotype $297.85 Heterozygous for Shhtm2(cre/ESR1)Cjt x Wild-type for Shhtm2(cre/ESR1)Cjt $297.85 Wild-type for Shhtm2(cre/ESR1)Cjt x Heterozygous for Shhtm2(cre/ESR1)Cjt
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $316.60 Female or Male Heterozygous for Shhtm2(cre/ESR1)Cjt
Pairs /Price (US dollars $) Pair Genotype $387.30 Heterozygous for Shhtm2(cre/ESR1)Cjt x Wild-type for Shhtm2(cre/ESR1)Cjt $387.30 Wild-type for Shhtm2(cre/ESR1)Cjt x Heterozygous for Shhtm2(cre/ESR1)Cjt
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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