Strain Name: |
B6.129S-Shhtm2(cre/ESR1)Cjt/J |
|---|---|
Stock Number: |
005623 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Shh; Shhtm2(cre/ESR1)Cjt; cre; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System +/+ sibling x Heterozygote (Female x Male) Species laboratory mouse Donating Investigator Clifford Tabin, Harvard Medical School Generation N5+1F2 (08-JAN-08) Strain Description
This strain expresses a fusion product involving Cre recombinase and a mutant form of the human estrogen receptor ligand binding domain from the endogenous Shh locus. The mutant human estrogen receptor does not bind natural ligand at physiological concentrations but will bind the synthetic ligand, 4-hydroxytamoxifen. Restricted to the cytoplasm, the Cre/ESR1 protein can only gain access to the nuclear compartment after exposure to tamoxifen. Tamoxifen administration induces Cre recombinase expression in all cells that express the endogenous gene resulting in the deletion of the first 35 base pairs following the ATG. Homozygous mice are not viable or fertile. Heterozygous mutant mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This mutant mouse strain may be useful in studies of limb patterning and development.Strain Development
A targeting vector containing a fusion product involving Cre recombinase and a mutant form of the human estrogen receptor ligand binding domain was inserted at the ATG of Shh. The construct was electroporated into 129S6/SvEv-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 5 generations.
Gene & Allele Details
| Allele Symbol | Shhtm2(cre/ESR1)Cjt | ||
|---|---|---|---|
| Allele Name | targeted mutation 2, Clifford J Tabin | ||
| Common Name(s) | Shh-cre; Shhtm2(cre-ERT2)Cjt; ShhcreERT2; | ||
| Mutation Made By | Clifford Tabin, Harvard Medical School | ||
| Site of Expression | tamoxifen inducible Cre recombinase protein fused to a mutant form of the human estrogen receptor ligand binding domain; expression in all cells that express the Shh gene | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Shh, sonic hedgehog | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | Dsh; HHG1; HLP3; HPE3; Hhg1; Hx; Hxl3; M100081; MCOPCB5; SMMCI; TPT; TPTPS; hedgehog gene 1; hemimelic extra toes; hemimelic extratoes like 3; short digits; | ||
| Molecular Note | A cassette containing CreERT2 was inserted at the ATG. Upon recombination, the first 35 base pairs after the ATG were deleted. Cre was produced in all cells in which the endogenous mRNA was normally expressed. [J:92504] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Shhtm2(cre/ESR1)Cjt
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, heterozygous mice are bred to wildtype siblings (or C57BL/6J). Homozygous mice are not viable. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Shh
004293 129-Shhtm2Amc/J 000214 B10.D2/nSn-ShhHx/J 005622 B6.Cg-Shhtm1(EGFP/cre)Cjt/J 003318 STOCK Shhtm1Amc/J View Strains carrying other alleles of Shh (4 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedDevelopmental Biology Research
Embryonic Lethality (Homozygous)
Limb Patterning Defects
Skeletal Defects
Research Tools
Cre-lox-System (Cre-Recombinase Expression: Inducible)
Developmental Biology Research (Cre-lox system)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
Research Tools
Cre-lox-System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
References
Selected Reference(s)
Additional ReferencesHarfe BD; Scherz PJ; Nissim S; Tian H; McMahon AP; Tabin CJ. 2004. Evidence for an expansion-based temporal Shh gradient in specifying vertebrate digit identities. Cell 118(4):517-28. [PubMed: 15315763] [J:92504]
Price and Supply Information
| Strain Name: | B6.129S-Shhtm2(cre/ESR1)Cjt/J |
| Stock Number: | 005623 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Notice Regarding cre/Esr1 (Tamoxifen Inducible Cre) Strains.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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