Strain Name:

FVB-Tg(Pcp2-tTA)3Horr/J

Stock Number:

005625

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Coisogenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Specieslaboratory mouse
 
Donating Investigator Robert Ehlenfeldt,   University of Minnesota

Description
Mice that are homozygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cerebellar Purkinje cell-specific tTA expression has been confirmed using PCR. Constitutive expression of tTA in Purkinje cells makes this strain suitable for creating bitransgenic mice that can, by withdrawing tetracycline (or its derivative doxycycline), inducibly express a gene of interest in Purkinje cells when the gene of interest is under the direction of a tetracycline-responsive element (TRE; tetO).

Development
A transgenic construct using the Purkinje cell-specific murine Pcp2 regulatory region to direct expression of the tetracycline-regulated transactivator, tTA, was injected into FVB/N embryos. These embryos were implanted into pseudopregnant FVB/N females. Founder animals were backcrossed to FVB/N for five generations and were maintained by intercross.

Control Information

  Control
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Pcp2     (5 strains)

Strains carrying other alleles of tTA
008079   129S-Ppargtm2Yba/J
016198   129S6.Cg-Tg(Camk2a-tTA)1Mmay/JlwsJ
011008   B6.129P2(Cg)-Gt(ROSA)26Sortm1(tTA)Roos/J
009602   B6.129S4(Cg)-Kcnn2tm2Jpad/J
009603   B6.129S4-Kcnn3tm1Jpad/J
008227   B6.129S4-Ppargtm3Yba/J
012359   B6.Cg-Pvalbtm1.1(tTA2)Hze/J
016868   B6.Cg-Ssttm1.2(tTA2)Hze/J
007004   B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ
003563   B6.Cg-Tg(Cebpb-tTA)5Bjd/J
003767   B6.Cg-Tg(Eno2tTA)5021Nes/J
003763   B6.Cg-Tg(Eno2tTA)5030Nes/J
018306   B6.Cg-Tg(Fos-tTA,Fos-EGFP*)1Mmay/J
005964   B6.Cg-Tg(GFAP-tTA)110Pop/J
002618   B6.Cg-Tg(MMTVtTA)1Mam/J
008284   B6.Cg-Tg(Scg2-tTA)1Jt/J
023970   B6.Cg-Tg(Sirpa-tTA)AUmri/J
023971   B6.Cg-Tg(Sirpa-tTA)SUmri/J
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
017722   B6.Cg-Tg(Tal1-tTA)19Dgt/J
017754   B6;129-Omptm1(tTA)Gogo/J
007585   B6;129S4-Npytm2Rpa/J
002709   B6;C3-Tg(TettTALuc)1Dgs/J
003010   B6;CBA-Tg(Camk2a-tTA)1Mmay/J
008344   B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J
010573   B6;SJL-Tg(Prl-tTA)6-5Jek/J
008082   B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J
008603   C.129P2(B6)-Gt(ROSA)26Sortm1(tTA)Roos/J
010712   C57BL/6-Tg(Camk2a-tTA)1Stl/J
013585   FVB-Tg(Cdh5-tTA)D5Lbjn/J
003170   FVB.Cg-Tg(Myh6-tTA)6Smbf/J
006209   FVB.Cg-Tg(Tal1-tTA)19Dgt/J
005942   FVB/N-Tg(Pf4-tTA/VP16)42Kra/J
004937   NOD.Cg-Tg(Ins2-tTA)1Doi/DoiJ
006999   STOCK Dbttm1Geh Tg(Cebpb-tTA)5Bjd Tg(tetO-DBT)A1Geh/J
008335   STOCK Foxa2tm1.1(rtTa)Moon/J
008600   STOCK Gt(ROSA)26Sortm1(tTA)Roos/J
005701   STOCK Pdx1tm1Macd/J
014092   STOCK Tg(ACTB-tTA2,-MAPT/lacZ)1Luo/J
003271   STOCK Tg(CMV-tTA)3Bjd/J
024854   STOCK Tg(Camk2a-tTA)1Mmay Tg(tetO-MAPT*P301L)#Kha/J
018124   STOCK Tg(Prnp-tTA)F959Sbp/J
009606   STOCK Tg(Six2-EGFP/cre)1Amc/J
003275   STOCK Tg(tetL)1Bjd/J
003274   STOCK Tg(tetNZL)2Bjd/J
016970   STOCK Tg(tetO-HCV)1Mlch/Mmjax
View Strains carrying other alleles of tTA     (46 strains)

Additional Web Information

Tet Expression Systems

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Cerebellar Defects
Tet Expression System
      tTA/rtTA Expressing Strains

Research Tools
Genetics Research
      Mutagenesis and Transgenesis
      Mutagenesis and Transgenesis: Tetop Tet System
Neurobiology Research
      Tetop Tet System
Tet Expression Systems
      tTA/rtTA Expressing Strains

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Pcp2-tTA)3Horr
Allele Name transgene insertion 3, Harry T Orr
Allele Type Transgenic (Inserted expressed sequence)
Mutation Made By Harry Orr,   University of Minnesota
Strain of OriginFVB/N
Site of ExpressionExpresses tTA in cerebellar Purkinje cells.
Expressed Gene tTA, tetracycline-controlled transactivator, E. coli
The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter.
Promoter Pcp2, Purkinje cell protein 2 (L7), mouse, laboratory
General Note Transgene mRNA levels in cerebella of mice of nine independent transgenic lines were assayed by Northern blot analysis of cerebellar extracts. The highest-expressing line was selected to cross with mice bearing a TetO-driven transgene.
Molecular Note This transgene expresses the tetracycline transactivator (tTA) under control of the 5' sequences, including the first exon, the first intron and part of the second exon, of the Purkinje-cell specific mouse Purkinje cell protein 2 gene. [MGI Ref ID J:95453]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Pcp2-tTA)3Horr, QPCR
Tg(tTA),

MELT


Tg(tTA),

Probe


Tg(tTA), Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Zu T; Duvick LA; Kaytor MD; Berlinger MS; Zoghbi HY; Clark HB; Orr HT. 2004. Recovery from polyglutamine-induced neurodegeneration in conditional SCA1 transgenic mice. J Neurosci 24(40):8853-61. [PubMed: 15470152]  [MGI Ref ID J:95453]

Additional References

Tg(Pcp2-tTA)3Horr related

Barnes JA; Ebner BA; Duvick LA; Gao W; Chen G; Orr HT; Ebner TJ. 2011. Abnormalities in the Climbing Fiber-Purkinje Cell Circuitry Contribute to Neuronal Dysfunction in ATXN1[82Q] Mice. J Neurosci 31(36):12778-89. [PubMed: 21900557]  [MGI Ref ID J:176576]

Baudouin SJ; Gaudias J; Gerharz S; Hatstatt L; Zhou K; Punnakkal P; Tanaka KF; Spooren W; Hen R; De Zeeuw CI; Vogt K; Scheiffele P. 2012. Shared synaptic pathophysiology in syndromic and nonsyndromic rodent models of autism. Science 338(6103):128-32. [PubMed: 22983708]  [MGI Ref ID J:187744]

Gatchel JR; Watase K; Thaller C; Carson JP; Jafar-Nejad P; Shaw C; Zu T; Orr HT; Zoghbi HY. 2008. The insulin-like growth factor pathway is altered in spinocerebellar ataxia type 1 and type 7. Proc Natl Acad Sci U S A 105(4):1291-6. [PubMed: 18216249]  [MGI Ref ID J:131821]

Oz G; Vollmers ML; Nelson CD; Shanley R; Eberly LE; Orr HT; Clark HB. 2011. In vivo monitoring of recovery from neurodegeneration in conditional transgenic SCA1 mice. Exp Neurol 232(2):290-8. [PubMed: 21963649]  [MGI Ref ID J:178469]

Serra HG; Duvick L; Zu T; Carlson K; Stevens S; Jorgensen N; Lysholm A; Burright E; Zoghbi HY; Clark HB; Andresen JM; Orr HT. 2006. RORalpha-mediated Purkinje cell development determines disease severity in adult SCA1 mice. Cell 127(4):697-708. [PubMed: 17110330]  [MGI Ref ID J:116123]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as homozygotes.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   001800 FVB/NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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