Strain Name: |
FVB-Tg(Pcp2-tTA)3Horr/J |
|---|---|
Stock Number: |
005625 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Pcp2; tTA; Tg(Pcp2-tTA)3Horr; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Robert Ehlenfeldt, University of Minnesota Strain Description
Mice that are homozygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Cerebellar Purkinje cell-specific tTA expression has been confirmed using PCR. Constitutive expression of tTA in Purkinje cells makes this strain suitable for creating bitransgenic mice that can, by withdrawing tetracycline (or its derivative doxycycline), inducibly express a gene of interest in Purkinje cells when the gene of interest is under the direction of a tetracycline-responsive element (TRE; tetO).Strain Development
A transgenic construct using the Purkinje cell-specific murine Pcp2 regulatory region to direct expression of the tetracycline-regulated transactivator, tTA, was injected into FVB/N embryos. These embryos were implanted into pseudopregnant FVB/N females. Founder animals were backcrossed to FVB/N for five generations and were maintained by intercross.
Gene & Allele Details
| Allele Symbol | Tg(Pcp2-tTA)3Horr | ||
|---|---|---|---|
| Allele Name | transgene insertion 3, Harry T Orr | ||
| Mutation Made By | Harry Orr, University of Minnesota | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | Expresses tTA in cerebellar Purkinje cells. | ||
| Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli | ||
| The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter. | |||
| Promoter | Pcp2, Purkinje cell protein 2 (L7), mouse, laboratory | ||
| General Note | Transgene mRNA levels in cerebella of mice of nine independent transgenic lines were assayed by Northern blot analysis of cerebellar extracts. The highest-expressing line was selected to cross with mice bearing a TetO-driven transgene. | ||
| Molecular Note | This transgene expresses the tetracycline transactivator (tTA) under control of the 5' sequences, including the first exon, the first intron and part of the second exon, of the Purkinje-cell specific mouse Purkinje cell protein 2 gene. [MGI Ref ID J:95453] | ||
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Pcp2-tTA)3Horr QPCR
Tg(tTA)
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as homozygotes. |
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Related Strains
Strains carrying other alleles of Pcp2
004146 B6.129-Tg(Pcp2-cre)2Mpin/J 004690 B6;FVB-Tg(Pcp2-EGFP)2Yuza/J 006207 STOCK Tg(Pcp2-cre)1Amc/J View Strains carrying other alleles of Pcp2 (3 strains)
007004 B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ 003767 B6.Cg-Tg(Eno2tTA)5021Nes/J 003763 B6.Cg-Tg(Eno2tTA)5030Nes/J 005964 B6.Cg-Tg(GFAP-tTA)110Pop/J 002618 B6.Cg-Tg(MMTVtTA)1Mam/J 006361 B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J 003563 B6.Cg-Tg(tTALap)5Bjd/J 002709 B6;C3-Tg(TettTALuc)1Dgs/J 003010 B6;CBA-Tg(Camk2a-tTA)1Mmay/J 008082 B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J 003170 FVB.Cg-Tg(Myh6-tTA)6Smbf/J 006209 FVB.Cg-Tg(Tal1-tTA)19Dgt/J 005942 FVB/N-Tg(Pf4-tTA/VP16)42Kra/J 004937 NOD.Cg-Tg(Ins2-tTA)1Doi/DoiJ 006999 STOCK Dbttm1Geh Tg(tTALap)5Bjd Tg(tetO-DBT)A1Geh/J 003272 STOCK Tg(tTALap)5Bjd/J 003271 STOCK Tg(tTAhCMV)3Bjd/J 003275 STOCK Tg(tetL)1Bjd/J 003274 STOCK Tg(tetNZL)2Bjd/J
Additional Web Information
Tet Expression Systems
Research Applications
This mouse can be used to support research in many areas including:
Neurobiology Research
Cerebellar Defects
Tet Expression System (tTA/rtTA Expressing Strains)
Research Tools
Genetics Research (Mutagenesis and Transgenesis: Tetop Tet System)
Neurobiology Research (Tetop Tet System)
Tet Expression Systems (tTA/rtTA Expressing Strains)
References
Selected Reference(s)
Additional ReferencesZu T; Duvick LA; Kaytor MD; Berlinger MS; Zoghbi HY; Clark HB; Orr HT. 2004. Recovery from polyglutamine-induced neurodegeneration in conditional SCA1 transgenic mice. J Neurosci 24(40):8853-61. [PubMed: 15470152] [MGI Ref ID J:95453]
Price and Supply Information
| Strain Name: | FVB-Tg(Pcp2-tTA)3Horr/J |
| Stock Number: | 005625 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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