Strain Name:

B6.129X1-Cxcl13tm1Cys/J

Stock Number:

005626

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   19-AUG-06
Specieslaboratory mouse
Generation?+N1p (15-JAN-06)
Generation Definitions
 
Donating Investigator Jason Cyster,   University of California San Francisco

Description
Mice that are homozygous for the targeted mutation are viable, fertile and normal in size. No endogenous gene product (mRNA or protein) is detected in spleen or mesenteric lymph node. EGFP is not expressed. Severe, but incompletely penetrant, defects of the inguinal, iliac, axillary, brachial, popliteal, deep cervical, renal, sacral, and parathymic lymph nodes are observed. The lymphoid patch of the cecum is absent and Peyer's patches are severely reduced and typically malformed. This mutant has defective B cell organization, an absence of primary follicle follicular dendritic cells, and reduced B cell LTa1b2 expression in spleen and lymph nodes. This mutant may be useful in B cell, follicular dendritic cell, and/or lymph node development studies.

Development
A targeting vector was constructed in which base pairs 18-116 of exon 2 from the endogenous gene were replaced with an in-frame stop codon, a Mengo virus internal ribosome entry site, an enhanced green fluorescent protein gene (EGFP), and a loxP-flanked neomycin resistance gene. The construct was electroporated into 129X1/SvJ derived JM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were backcrossed for germ-line transmission to C57BL/6J females. Offspring were mated with Cre-expressing C57BL/6J to remove the neomycin resistance gene. The neo-excised heterozygotes were backcrossed to C57BL/6J for ten generations before being made homozygous.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Cxcl13
006154   NOD.Cg-Tg(Ins2-Cxcl13)1Cys/JbsJ
View Strains carrying other alleles of Cxcl13     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Cxcl13tm1Cys/Cxcl13tm1Cys

        B6.129X1-Cxcl13tm1Cys
  • immune system phenotype
  • abnormal B cell physiology
    • germinal center (GC) B cells migrate more slowly in culture than wild-type GC B cells; cells show a slower velocity and less mean displacement over time compared to wild-type cells   (MGI Ref ID J:118931)
  • hematopoietic system phenotype
  • abnormal B cell physiology
    • germinal center (GC) B cells migrate more slowly in culture than wild-type GC B cells; cells show a slower velocity and less mean displacement over time compared to wild-type cells   (MGI Ref ID J:118931)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Cxcl13tm1Cys/Cxcl13tm1Cys

        involves: 129X1/SvJ * C57BL/6
  • immune system phenotype
  • abnormal B cell physiology
    • impaired trafficking of B cells to lymphoid follicles   (MGI Ref ID J:78282)
  • abnormal Peyer's patch morphology
    • Peyer's patches that are observed lack the characteristic multi-domed structure   (MGI Ref ID J:78282)
    • decreased Peyer's patch number   (MGI Ref ID J:78282)
    • small Peyer's patches
      • Peyer's patches that are observed are small   (MGI Ref ID J:78282)
  • abnormal lymph node germinal center morphology
    • germinal centers form in lymph nodes after immunization with T-cell dependent antigen, however they are misplaced and smaller   (MGI Ref ID J:78282)
  • abnormal spleen B cell follicle morphology
    • B cells fail to organize into polarized follicular clusters and appear as a ring of cells at the perimeter of T-cell areas   (MGI Ref ID J:78282)
    • boundary between B-cell rich areas and T zones are often poorly demarcated, with increased numbers of B cells and T cells in reciprocal areas   (MGI Ref ID J:78282)
    • decreased spleen germinal center size
      • germinal centers form in spleen after immunization with T-cell dependent antigen, however they are misplaced and smaller   (MGI Ref ID J:78282)
  • abnormal spleen marginal zone morphology
    • spleen shows a thickened ring of IgMhiIgDlo marginal zone B cells and the boundary between the B cells in this area and the inner ring of B cells is disrupted, with increased numbers of IgMhiIgDlo cells in the outer marginal zone   (MGI Ref ID J:78282)
  • absent axillary lymph nodes
    • in most mice   (MGI Ref ID J:78282)
  • absent brachial lymph nodes
    • in most mice   (MGI Ref ID J:78282)
  • absent follicular dendritic cells
    • absence of primary follicular dendritic cells in spleen and lymph nodes   (MGI Ref ID J:78282)
  • absent inguinal lymph nodes
    • in most mice   (MGI Ref ID J:78282)
  • decreased lymph node number
    • most mice lack inguinal, iliac, sacral, brachial, and axillary lymph nodes   (MGI Ref ID J:78282)
    • a full set of mesenteric lymph nodes were observed however, a full set of mesenteric lymph nodes is observed   (MGI Ref ID J:78282)
  • hematopoietic system phenotype
  • abnormal B cell physiology
    • impaired trafficking of B cells to lymphoid follicles   (MGI Ref ID J:78282)
  • abnormal spleen B cell follicle morphology
    • B cells fail to organize into polarized follicular clusters and appear as a ring of cells at the perimeter of T-cell areas   (MGI Ref ID J:78282)
    • boundary between B-cell rich areas and T zones are often poorly demarcated, with increased numbers of B cells and T cells in reciprocal areas   (MGI Ref ID J:78282)
    • decreased spleen germinal center size
      • germinal centers form in spleen after immunization with T-cell dependent antigen, however they are misplaced and smaller   (MGI Ref ID J:78282)
  • abnormal spleen marginal zone morphology
    • spleen shows a thickened ring of IgMhiIgDlo marginal zone B cells and the boundary between the B cells in this area and the inner ring of B cells is disrupted, with increased numbers of IgMhiIgDlo cells in the outer marginal zone   (MGI Ref ID J:78282)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Internal/Organ Defects
      Lymphoid Tissue Defects
Lymphoid Tissue Defects
      hematopoietic defects

Immunology, Inflammation and Autoimmunity Research
Lymphoid Tissue Defects
      hematopoietic development

Internal/Organ Research
Lymphoid Tissue Defects

Research Tools
Immunology, Inflammation and Autoimmunity Research
      B cell deficiency

Cxcl13tm1Cys related

Developmental Biology Research
Internal/Organ Defects
      Lymphoid Tissue Defects
Lymphoid Tissue Defects

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
      B cell defects
Lymphoid Tissue Defects
      Lymphocyte Homing
      selective lymph node development defects

Internal/Organ Research
Lymphoid Tissue Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Cxcl13tm1Cys
Allele Name targeted mutation 1, Jason G Cyster
Allele Type Targeted (Null/Knockout, Reporter)
Common Name(s) BLC-; CXCL13-;
Mutation Made By Jason Cyster,   University of California San Francisco
Strain of Origin129X1/SvJ
ES Cell Line NameJM-1
ES Cell Line Strain129X1/SvJ
Gene Symbol and Name Cxcl13, chemokine (C-X-C motif) ligand 13
Chromosome 5
Gene Common Name(s) 4631412M08Rik; ANGIE; ANGIE2; BCA-1; BCA1; BLC; BLR1L; RIKEN cDNA 4631412M08 gene; SCYB13; Scyb13; small inducible cytokine subfamily B (Cys-X-Cys), member 13;
Molecular Note Base pairs 18 through 116 (exon 2) were replaced by a cassette containing a stop codon followed by an internal ribosome entry site, an enhanced green fluorescent protein gene, and a floxed neo gene. The deleted region encoded amino acids 27 through 60, including 3 of the 4 conserved Cys residues. The absence of transcript and protein in homozygous mutant mice was confirmed by Northern and Western blot analyses of homozygous mutant mice. [MGI Ref ID J:78282]

Genotyping

Genotyping Information

Genotyping Protocols

Cxcl13tm1Cys, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Ansel KM; Ngo VN; Hyman PL; Luther SA; Forster R; Sedgwick JD; Browning JL; Lipp M; Cyster JG. 2000. A chemokine-driven positive feedback loop organizes lymphoid follicles. Nature 406(6793):309-14. [PubMed: 10917533]  [MGI Ref ID J:78282]

Additional References

Cxcl13tm1Cys related

Allen CD; Okada T; Tang HL; Cyster JG. 2007. Imaging of germinal center selection events during affinity maturation. Science 315(5811):528-31. [PubMed: 17185562]  [MGI Ref ID J:118931]

Ansel KM; Harris RB; Cyster JG. 2002. CXCL13 is required for B1 cell homing, natural antibody production, and body cavity immunity. Immunity 16(1):67-76. [PubMed: 11825566]  [MGI Ref ID J:111521]

Bagaeva LV; Rao P; Powers JM; Segal BM. 2006. CXC chemokine ligand 13 plays a role in experimental autoimmune encephalomyelitis. J Immunol 176(12):7676-85. [PubMed: 16751415]  [MGI Ref ID J:132350]

Cinamon G; Matloubian M; Lesneski MJ; Xu Y; Low C; Lu T; Proia RL; Cyster JG. 2004. Sphingosine 1-phosphate receptor 1 promotes B cell localization in the splenic marginal zone. Nat Immunol 5(7):713-20. [PubMed: 15184895]  [MGI Ref ID J:91161]

Cinamon G; Zachariah MA; Lam OM; Foss FW Jr; Cyster JG. 2008. Follicular shuttling of marginal zone B cells facilitates antigen transport. Nat Immunol 9(1):54-62. [PubMed: 18037889]  [MGI Ref ID J:130200]

Colombo MJ; Sun G; Alugupalli KR. 2010. T-cell-independent immune responses do not require CXC ligand 13-mediated B1 cell migration. Infect Immun 78(9):3950-6. [PubMed: 20584971]  [MGI Ref ID J:163016]

Cupedo T; Lund FE; Ngo VN; Randall TD; Jansen W; Greuter MJ; de Waal-Malefyt R; Kraal G; Cyster JG; Mebius RE. 2004. Initiation of cellular organization in lymph nodes is regulated by non-B cell-derived signals and is not dependent on CXC chemokine ligand 13. J Immunol 173(8):4889-96. [PubMed: 15470030]  [MGI Ref ID J:93711]

Eberl G; Marmon S; Sunshine MJ; Rennert PD; Choi Y; Littman DR. 2004. An essential function for the nuclear receptor RORgamma(t) in the generation of fetal lymphoid tissue inducer cells. Nat Immunol 5(1):64-73. [PubMed: 14691482]  [MGI Ref ID J:87395]

Egawa T; Eberl G; Taniuchi I; Benlagha K; Geissmann F; Hennighausen L; Bendelac A; Littman DR. 2005. Genetic evidence supporting selection of the valpha14i NKT cell lineage from double-positive thymocyte precursors. Immunity 22(6):705-16. [PubMed: 15963785]  [MGI Ref ID J:99111]

Green JA; Suzuki K; Cho B; Willison LD; Palmer D; Allen CD; Schmidt TH; Xu Y; Proia RL; Coughlin SR; Cyster JG. 2011. The sphingosine 1-phosphate receptor S1P maintains the homeostasis of germinal center B cells and promotes niche confinement. Nat Immunol 12(7):672-80. [PubMed: 21642988]  [MGI Ref ID J:174313]

Ha SA; Tsuji M; Suzuki K; Meek B; Yasuda N; Kaisho T; Fagarasan S. 2006. Regulation of B1 cell migration by signals through Toll-like receptors. J Exp Med 203(11):2541-50. [PubMed: 17060475]  [MGI Ref ID J:124630]

Khader SA; Rangel-Moreno J; Fountain JJ; Martino CA; Reiley WW; Pearl JE; Winslow GM; Woodland DL; Randall TD; Cooper AM. 2009. In a murine tuberculosis model, the absence of homeostatic chemokines delays granuloma formation and protective immunity. J Immunol 183(12):8004-14. [PubMed: 19933855]  [MGI Ref ID J:157487]

Luther SA; Ansel KM; Cyster JG. 2003. Overlapping roles of CXCL13, interleukin 7 receptor alpha, and CCR7 ligands in lymph node development. J Exp Med 197(9):1191-8. [PubMed: 12732660]  [MGI Ref ID J:83288]

McDonald KG; McDonough JS; Dieckgraefe BK; Newberry RD. 2010. Dendritic cells produce CXCL13 and participate in the development of murine small intestine lymphoid tissues. Am J Pathol 176(5):2367-77. [PubMed: 20304952]  [MGI Ref ID J:160774]

Pereira JP; Kelly LM; Xu Y; Cyster JG. 2009. EBI2 mediates B cell segregation between the outer and centre follicle. Nature :. [PubMed: 19597478]  [MGI Ref ID J:151077]

Publicover J; Gaggar A; Nishimura S; Van Horn CM; Goodsell A; Muench MO; Reinhardt RL; van Rooijen N; Wakil AE; Peters M; Cyster JG; Erle DJ; Rosenthal P; Cooper S; Baron JL. 2013. Age-dependent hepatic lymphoid organization directs successful immunity to hepatitis B. J Clin Invest 123(9):3728-39. [PubMed: 23925290]  [MGI Ref ID J:201620]

Rangel-Moreno J; Moyron-Quiroz J; Kusser K; Hartson L; Nakano H; Randall TD. 2005. Role of CXC chemokine ligand 13, CC chemokine ligand (CCL) 19, and CCL21 in the organization and function of nasal-associated lymphoid tissue. J Immunol 175(8):4904-13. [PubMed: 16210592]  [MGI Ref ID J:119053]

Rangel-Moreno J; Moyron-Quiroz JE; Carragher DM; Kusser K; Hartson L; Moquin A; Randall TD. 2009. Omental milky spots develop in the absence of lymphoid tissue-inducer cells and support B and T cell responses to peritoneal antigens. Immunity 30(5):731-43. [PubMed: 19427241]  [MGI Ref ID J:149548]

Rangel-Moreno J; Moyron-Quiroz JE; Hartson L; Kusser K; Randall TD. 2007. Pulmonary expression of CXC chemokine ligand 13, CC chemokine ligand 19, and CC chemokine ligand 21 is essential for local immunity to influenza. Proc Natl Acad Sci U S A 104(25):10577-82. [PubMed: 17563386]  [MGI Ref ID J:143828]

Slight SR; Rangel-Moreno J; Gopal R; Lin Y; Fallert Junecko BA; Mehra S; Selman M; Becerril-Villanueva E; Baquera-Heredia J; Pavon L; Kaushal D; Reinhart TA; Randall TD; Khader SA. 2013. CXCR5+ T helper cells mediate protective immunity against tuberculosis. J Clin Invest :. [PubMed: 23281399]  [MGI Ref ID J:194502]

Yu P; Wang Y; Chin RK; Martinez-Pomares L; Gordon S; Kosco-Vibois MH; Cyster J; Fu YX. 2002. B cells control the migration of a subset of dendritic cells into B cell follicles via CXC chemokine ligand 13 in a lymphotoxin-dependent fashion. J Immunol 168(10):5117-23. [PubMed: 11994465]  [MGI Ref ID J:127080]

van de Pavert SA; Olivier BJ; Goverse G; Vondenhoff MF; Greuter M; Beke P; Kusser K; Hopken UE; Lipp M; Niederreither K; Blomhoff R; Sitnik K; Agace WW; Randall TD; de Jonge WJ; Mebius RE. 2009. Chemokine CXCL13 is essential for lymph node initiation and is induced by retinoic acid and neuronal stimulation. Nat Immunol 10(11):1193-9. [PubMed: 19783990]  [MGI Ref ID J:157747]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are maintained as homozygotes. Despite defective B cell function and lymph node development, the mutants live and breed normally in SPF vivaria.
Mating SystemHomozygote x Homozygote         (Female x Male)   19-AUG-06

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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