Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse   Donating Investigator Jason Cyster,   University of California San Francisco

Description
Mice that are homozygous for the targeted mutation are viable, fertile and normal in size. No endogenous gene product (mRNA or protein) is detected in spleen or mesenteric lymph node. EGFP is not expressed. Severe, but incompletely penetrant, defects of the inguinal, iliac, axillary, brachial, popliteal, deep cervical, renal, sacral, and parathymic lymph nodes are observed. The lymphoid patch of the cecum is absent and Peyer's patches are severely reduced and typically malformed. This mutant has defective B cell organization, an absence of primary follicle follicular dendritic cells, and reduced B cell LTa1b2 expression in spleen and lymph nodes. This mutant may be useful in B cell, follicular dendritic cell, and/or lymph node development studies.

Development
A targeting vector was constructed in which base pairs 18-116 of exon 2 from the endogenous gene were replaced with an in-frame stop codon, a Mengo virus internal ribosome entry site, an enhanced green fluorescent protein gene (EGFP), and a loxP-flanked neomycin resistance gene. The construct was electroporated into 129X1/SvJ derived JM-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts and the resulting chimeric males were backcrossed for germ-line transmission to C57BL/6J females. Offspring were mated with Cre-expressing C57BL/6J to remove the neomycin resistance gene. The neo-excised heterozygotes were backcrossed to C57BL/6J for ten generations before being made homozygous.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Cxcl13

006154

NOD.Cg-Tg(Ins2-Cxcl13)1Cys/JbsJ

View Strains carrying other alleles of Cxcl13     (1 strain)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Cxcl13^tm1Cys/Cxcl13^tm1Cys

        B6.129X1-Cxcl13^tm1Cys

• immune system phenotype
• abnormal B cell physiology (MGI Ref ID J:118931)
  • germinal center (GC) B cells migrate more slowly in culture than wild-type GC B cells; cells show a slower velocity and less mean displacement over time compared to wild-type cells

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Cxcl13^tm1Cys/Cxcl13^tm1Cys

        involves: 129X1/SvJ * C57BL/6

• immune system phenotype
• abnormal B cell physiology (MGI Ref ID J:78282)
  • impaired trafficking of B cells to lymphoid follicles
• abnormal Peyer's patch morphology (MGI Ref ID J:78282)
  • Peyer's patches that were observed lacked the characteristic multi-domed structure
• small Peyer's patches (MGI Ref ID J:78282)
  • Peyer's patches that were observed were small
• decreased lymph node number (MGI Ref ID J:78282)
  • most mice lacked inguinal, iliac, sacral, brachial, and axillary lymph nodes
  • a full set of mesenteric lymph nodes were observed

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Internal/Organ Defects (Lymphoid Tissue Defects)
Lymphoid Tissue Defects (hematopoietic defects)

Immunology and Inflammation Research
Lymphoid Tissue Defects (hematopoietic development)

Internal/Organ Research
Lymphoid Tissue Defects

Research Tools
Immunology and Inflammation Research (B cell deficiency)

Cxcl13^tm1Cys related

Developmental Biology Research
Internal/Organ Defects (Lymphoid Tissue Defects)
Lymphoid Tissue Defects

Immunology and Inflammation Research
Immunodeficiency (B cell defects)
Lymphoid Tissue Defects (Lymphocyte Homing)
Lymphoid Tissue Defects (selective lymph node development defects)

Internal/Organ Research
Lymphoid Tissue Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Cxcl13tm1Cys
Allele Name		targeted mutation 1, Jason G Cyster
Allele Type		Targeted (Reporter)
Common Name(s)		BLC-; CXCL13-;
Mutation Made By		Jason Cyster,   University of California San Francisco
Strain of Origin		129X1/SvJ
ES Cell Line Name		JM-1
ES Cell Line Strain		129X1/SvJ
Gene Symbol and Name		Cxcl13, chemokine (C-X-C motif) ligand 13
Chromosome		5
Gene Common Name(s)		ANGIE; ANGIE2; BCA-1; BCA1; BLC; BLR1L; SCYB13; Scyb13; small inducible cytokine subfamily B (Cys-X-Cys), member 13;
Molecular Note		Base pairs 18 through 116 (exon 2) were deleted by the insertion of a cassette containing a stop codon followed by an internal ribosome entry site, an enhanced green fluorescent protein gene, and a floxed neo gene. The deleted region encoded amino acids 27 through 60, including 3 of the 4 conserved Cys residues. The absence of transcript and protein in homozygous mutant mice was confirmed by Northern and Western blot analyses of homozygous mutant mice. [MGI Ref ID J:78282]

Genotyping

Genotyping Information

Genotyping Protocols

Cxcl13^tm1Cys, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Ansel KM; Ngo VN; Hyman PL; Luther SA; Forster R; Sedgwick JD; Browning JL; Lipp M; Cyster JG. 2000. A chemokine-driven positive feedback loop organizes lymphoid follicles. Nature 406(6793):309-14. [PubMed: 10917533]  [MGI Ref ID J:78282]

Additional References

Cxcl13^tm1Cys related

Allen CD; Okada T; Tang HL; Cyster JG. 2007. Imaging of germinal center selection events during affinity maturation. Science 315(5811):528-31. [PubMed: 17185562]  [MGI Ref ID J:118931]

Ansel KM; Harris RB; Cyster JG. 2002. CXCL13 is required for B1 cell homing, natural antibody production, and body cavity immunity. Immunity 16(1):67-76. [PubMed: 11825566]  [MGI Ref ID J:111521]

Bagaeva LV; Rao P; Powers JM; Segal BM. 2006. CXC chemokine ligand 13 plays a role in experimental autoimmune encephalomyelitis. J Immunol 176(12):7676-85. [PubMed: 16751415]  [MGI Ref ID J:132350]

Cinamon G; Matloubian M; Lesneski MJ; Xu Y; Low C; Lu T; Proia RL; Cyster JG. 2004. Sphingosine 1-phosphate receptor 1 promotes B cell localization in the splenic marginal zone. Nat Immunol 5(7):713-20. [PubMed: 15184895]  [MGI Ref ID J:91161]

Cinamon G; Zachariah MA; Lam OM; Foss FW Jr; Cyster JG. 2008. Follicular shuttling of marginal zone B cells facilitates antigen transport. Nat Immunol 9(1):54-62. [PubMed: 18037889]  [MGI Ref ID J:130200]

Cupedo T; Lund FE; Ngo VN; Randall TD; Jansen W; Greuter MJ; de Waal-Malefyt R; Kraal G; Cyster JG; Mebius RE. 2004. Initiation of cellular organization in lymph nodes is regulated by non-B cell-derived signals and is not dependent on CXC chemokine ligand 13. J Immunol 173(8):4889-96. [PubMed: 15470030]  [MGI Ref ID J:93711]

Eberl G; Marmon S; Sunshine MJ; Rennert PD; Choi Y; Littman DR. 2004. An essential function for the nuclear receptor RORgamma(t) in the generation of fetal lymphoid tissue inducer cells. Nat Immunol 5(1):64-73. [PubMed: 14691482]  [MGI Ref ID J:87395]

Egawa T; Eberl G; Taniuchi I; Benlagha K; Geissmann F; Hennighausen L; Bendelac A; Littman DR. 2005. Genetic evidence supporting selection of the valpha14i NKT cell lineage from double-positive thymocyte precursors. Immunity 22(6):705-16. [PubMed: 15963785]  [MGI Ref ID J:99111]

Ha SA; Tsuji M; Suzuki K; Meek B; Yasuda N; Kaisho T; Fagarasan S. 2006. Regulation of B1 cell migration by signals through Toll-like receptors. J Exp Med 203(11):2541-50. [PubMed: 17060475]  [MGI Ref ID J:124630]

Luther SA; Ansel KM; Cyster JG. 2003. Overlapping roles of CXCL13, interleukin 7 receptor alpha, and CCR7 ligands in lymph node development. J Exp Med 197(9):1191-8. [PubMed: 12732660]  [MGI Ref ID J:83288]

Rangel-Moreno J; Moyron-Quiroz J; Kusser K; Hartson L; Nakano H; Randall TD. 2005. Role of CXC chemokine ligand 13, CC chemokine ligand (CCL) 19, and CCL21 in the organization and function of nasal-associated lymphoid tissue. J Immunol 175(8):4904-13. [PubMed: 16210592]  [MGI Ref ID J:119053]

Yu P; Wang Y; Chin RK; Martinez-Pomares L; Gordon S; Kosco-Vibois MH; Cyster J; Fu YX. 2002. B cells control the migration of a subset of dendritic cells into B cell follicles via CXC chemokine ligand 13 in a lymphotoxin-dependent fashion. J Immunol 168(10):5117-23. [PubMed: 11994465]  [MGI Ref ID J:127080]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are maintained as homozygotes. Despite defective B cell function and lymph node development, the mutants live and breed normally in SPF vivaria.
Mating System	Homozygote x Homozygote         (Female x Male)

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.