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| This strain expresses Cre recombinase from the endogenous Emx1 locus, and when crossed with a strain containing a loxP-site flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in approximately 88% of the neurons of the neocortex and hippocampus, and in the glial cells of the pallium. This mutant mouse strain represents a model that may be useful in studies of forebrain development and function. | |||||||||||||||
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Description
Strain Information
Former Names B6.Cg-Emx1tm1(cre)Krj/J (Changed: 27-MAR-08 ) B6.129-Emx1tm1(cre)Krj/J (Changed: 27-MAR-08 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 02-NOV-06 Species laboratory mouse Generation N12+F16 (18-JUN-12)
Generation DefinitionsDonating Investigator Kevin R. Jones, University of Colorado- Boulder Description
Mice homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This strain expresses Cre recombinase from the endogenous Emx1 locus. Western blot analysis of cortical brain tissue does not detect reduced endogenous gene product (protein). When crossed with a strain containing a loxP-site flanked sequence, Cre-mediated recombination results in tissue-specific deletion of the flanked sequence. Recombination occurs in approximately 88% of the neurons of the neocortex and hippocampus, and in the glial cells of the pallium, mimicking the pattern of expression of the endogenous gene. Further, the donating investigator reports that Cre recombinase is also expressed in a subset of male germline cells, thus some offspring from a cre; floxed male will have the floxed allele recombined in all cells. This mutant mouse strain represents a model that may be useful in studies of forebrain development and function.View cre expression characterization.
Development
A targeting vector containing an internal ribosome entry site (IRES) sequence, Cre coding sequence, a polyadenlyation signal and a FRT site flanked PGK-neomycin cassette was inserted 120bp 3' from the stop codon, in the 3' untranslated region. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animal was crossed to a Flp recombinase expressing strain (FLP-4917) to remove the selection cassette. The mice were then backcrossed to C57BL/6 mice for 12 generations.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Emx1
003080 B6;129X1-Emx1tm1Jlr/J View Strains carrying other alleles of Emx1 (1 strain)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Emx1tm1(cre)Krj/Emx1tm1(cre)Krj
involves: 129S2/SvPas * C57BL/6
- normal phenotype
- no abnormal phenotype detected
- homozygous mice do not show any of the abnormal phenotypes shown for Emx1tm1Jlr homozygotes (MGI Ref ID J:78098)
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Neurodevelopmental Defects
Research Tools
Cre-lox System
Cre Recombinase Expression
Cre Recombinase Expression: Germline/Embryonic Expression
Developmental Biology Research
Cre-lox System
Neurobiology Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Emx1tm1(cre)Krj | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Kevin R Jones | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Emx1-IRES-Cre; Emx1IREScre; emx1-cre; | ||
| Mutation Made By | Kevin Jones, University of Colorado- Boulder | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Site of Expression | neurons of the neocortex and hippocampus | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Driver Note | Emx1 | ||
| Molecular Note | An IRES-cre gene followed by an FRT flanked PGK-neomycin gene was inserted 120 bp 3' of the stop codon. The neomycin gene was later removed in vivo by crossing to an Flp recombinase expressing transgenic strain. The protein product of this gene was produced in normal quantities as confirmed by Western blot analysis. Cre recombinase expression was characterized by crossing to a Rosa26 strain. [MGI Ref ID J:78098] | ||
| Gene Symbol and Name | Emx1, empty spiracles homeobox 1 | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | RGD1564002; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Emx1tm1(cre)Krj, Standard PCR
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Gorski JA; Talley T; Qiu M; Puelles L; Rubenstein JL; Jones KR. 2002. Cortical excitatory neurons and glia, but not GABAergic neurons, are produced in the Emx1-expressing lineage. J Neurosci 22(15):6309-14. [PubMed: 12151506] [MGI Ref ID J:78098]
Additional References
Emx1tm1(cre)Krj relatedAlfano C; Viola L; Heng JI; Pirozzi M; Clarkson M; Flore G; De Maio A; Schedl A; Guillemot F; Studer M. 2011. COUP-TFI promotes radial migration and proper morphology of callosal projection neurons by repressing Rnd2 expression. Development 138(21):4685-97. [PubMed: 21965613] [MGI Ref ID J:178862]
Armentano M; Chou SJ; Tomassy GS; Leingartner A; O'Leary DD; Studer M. 2007. COUP-TFI regulates the balance of cortical patterning between frontal/motor and sensory areas. Nat Neurosci 10(10):1277-86. [PubMed: 17828260] [MGI Ref ID J:127387]
Armour EA; Carson RP; Ess KC. 2012. Cystogenesis and elongated primary cilia in Tsc1-deficient distal convoluted tubules. Am J Physiol Renal Physiol 303(4):F584-92. [PubMed: 22674026] [MGI Ref ID J:188431]
Ballester-Rosado CJ; Albright MJ; Wu CS; Liao CC; Zhu J; Xu J; Lee LJ; Lu HC. 2010. mGluR5 in cortical excitatory neurons exerts both cell-autonomous and -nonautonomous influences on cortical somatosensory circuit formation. J Neurosci 30(50):16896-909. [PubMed: 21159961] [MGI Ref ID J:167595]
Baquet ZC; Gorski JA; Jones KR. 2004. Early striatal dendrite deficits followed by neuron loss with advanced age in the absence of anterograde cortical brain-derived neurotrophic factor. J Neurosci 24(17):4250-8. [PubMed: 15115821] [MGI Ref ID J:96903]
Bareyre FM; Kerschensteiner M; Misgeld T; Sanes JR. 2005. Transgenic labeling of the corticospinal tract for monitoring axonal responses to spinal cord injury. Nat Med 11(12):1355-60. [PubMed: 16286922] [MGI Ref ID J:123607]
Barnes AP; Lilley BN; Pan YA; Plummer LJ; Powell AW; Raines AN; Sanes JR; Polleux F. 2007. LKB1 and SAD kinases define a pathway required for the polarization of cortical neurons. Cell 129(3):549-63. [PubMed: 17482548] [MGI Ref ID J:162066]
Barros CS; Calabrese B; Chamero P; Roberts AJ; Korzus E; Lloyd K; Stowers L; Mayford M; Halpain S; Muller U. 2009. Impaired maturation of dendritic spines without disorganization of cortical cell layers in mice lacking NRG1/ErbB signaling in the central nervous system. Proc Natl Acad Sci U S A 106(11):4507-12. [PubMed: 19240213] [MGI Ref ID J:146779]
Beggs HE; Schahin-Reed D; Zang K; Goebbels S; Nave KA; Gorski J; Jones KR; Sretavan D; Reichardt LF. 2003. FAK deficiency in cells contributing to the basal lamina results in cortical abnormalities resembling congenital muscular dystrophies. Neuron 40(3):501-14. [PubMed: 14642275] [MGI Ref ID J:86453]
Benadiba C; Magnani D; Niquille M; Morle L; Valloton D; Nawabi H; Ait-Lounis A; Otsmane B; Reith W; Theil T; Hornung JP; Lebrand C; Durand B. 2012. The ciliogenic transcription factor RFX3 regulates early midline distribution of guidepost neurons required for corpus callosum development. PLoS Genet 8(3):e1002606. [PubMed: 22479201] [MGI Ref ID J:183848]
Berger J; Berger S; Tuoc TC; D'Amelio M; Cecconi F; Gorski JA; Jones KR; Gruss P; Stoykova A. 2007. Conditional activation of Pax6 in the developing cortex of transgenic mice causes progenitor apoptosis. Development 134(7):1311-22. [PubMed: 17329367] [MGI Ref ID J:135032]
Betley JN; Wright CV; Kawaguchi Y; Erdelyi F; Szabo G; Jessell TM; Kaltschmidt JA. 2009. Stringent specificity in the construction of a GABAergic presynaptic inhibitory circuit. Cell 139(1):161-74. [PubMed: 19804761] [MGI Ref ID J:157313]
Bloom AJ; Miller BR; Sanes JR; Diantonio A. 2007. The requirement for Phr1 in CNS axon tract formation reveals the corticostriatal boundary as a choice point for cortical axons. Genes Dev 21(20):2593-606. [PubMed: 17901218] [MGI Ref ID J:125702]
Boretius S; Michaelis T; Tammer R; Ashery-Padan R; Frahm J; Stoykova A. 2009. In vivo MRI of altered brain anatomy and fiber connectivity in adult pax6 deficient mice. Cereb Cortex 19(12):2838-47. [PubMed: 19329571] [MGI Ref ID J:174170]
Brinkmann BG; Agarwal A; Sereda MW; Garratt AN; Muller T; Wende H; Stassart RM; Nawaz S; Humml C; Velanac V; Radyushkin K; Goebbels S; Fischer TM; Franklin RJ; Lai C; Ehrenreich H; Birchmeier C; Schwab MH; Nave KA. 2008. Neuregulin-1/ErbB signaling serves distinct functions in myelination of the peripheral and central nervous system. Neuron 59(4):581-95. [PubMed: 18760695] [MGI Ref ID J:145464]
Brown KN; Chen S; Han Z; Lu CH; Tan X; Zhang XJ; Ding L; Lopez-Cruz A; Saur D; Anderson SA; Huang K; Shi SH. 2011. Clonal production and organization of inhibitory interneurons in the neocortex. Science 334(6055):480-6. [PubMed: 22034427] [MGI Ref ID J:177854]
Caronia G; Wilcoxon J; Feldman P; Grove EA. 2010. Bone morphogenetic protein signaling in the developing telencephalon controls formation of the hippocampal dentate gyrus and modifies fear-related behavior. J Neurosci 30(18):6291-301. [PubMed: 20445055] [MGI Ref ID J:160559]
Caronia-Brown G; Grove EA. 2011. Timing of cortical interneuron migration is influenced by the cortical hem. Cereb Cortex 21(4):748-55. [PubMed: 20713502] [MGI Ref ID J:181828]
Carson RP; Fu C; Winzenburger P; Ess KC. 2013. Deletion of Rictor in neural progenitor cells reveals contributions of mTORC2 signaling to tuberous sclerosis complex. Hum Mol Genet 22(1):140-52. [PubMed: 23049074] [MGI Ref ID J:191137]
Carson RP; Van Nielen DL; Winzenburger PA; Ess KC. 2012. Neuronal and glia abnormalities in Tsc1-deficient forebrain and partial rescue by rapamycin. Neurobiol Dis 45(1):369-80. [PubMed: 21907282] [MGI Ref ID J:179842]
Chou SJ; Perez-Garcia CG; Kroll TT; O'Leary DD. 2009. Lhx2 specifies regional fate in Emx1 lineage of telencephalic progenitors generating cerebral cortex. Nat Neurosci 12(11):1381-9. [PubMed: 19820705] [MGI Ref ID J:154611]
Cocas LA; Miyoshi G; Carney RS; Sousa VH; Hirata T; Jones KR; Fishell G; Huntsman MM; Corbin JG. 2009. Emx1-lineage progenitors differentially contribute to neural diversity in the striatum and amygdala. J Neurosci 29(50):15933-46. [PubMed: 20016109] [MGI Ref ID J:157097]
Depaepe V; Suarez-Gonzalez N; Dufour A; Passante L; Gorski JA; Jones KR; Ledent C; Vanderhaeghen P. 2005. Ephrin signalling controls brain size by regulating apoptosis of neural progenitors. Nature 435(7046):1244-50. [PubMed: 15902206] [MGI Ref ID J:99355]
Elia LP; Yamamoto M; Zang K; Reichardt LF. 2006. p120 catenin regulates dendritic spine and synapse development through Rho-family GTPases and cadherins. Neuron 51(1):43-56. [PubMed: 16815331] [MGI Ref ID J:110469]
English CN; Vigers AJ; Jones KR. 2012. Genetic evidence that brain-derived neurotrophic factor mediates competitive interactions between individual cortical neurons. Proc Natl Acad Sci U S A 109(47):19456-61. [PubMed: 23129644] [MGI Ref ID J:192261]
Fuentes F; Zimmer D; Atienza M; Schottenfeld J; Penkala I; Bale T; Bence KK; Arregui CO. 2012. Protein tyrosine phosphatase PTP1B is involved in hippocampal synapse formation and learning. PLoS One 7(7):e41536. [PubMed: 22844492] [MGI Ref ID J:189720]
Garrett AM; Schreiner D; Lobas MA; Weiner JA. 2012. gamma-protocadherins control cortical dendrite arborization by regulating the activity of a FAK/PKC/MARCKS signaling pathway. Neuron 74(2):269-76. [PubMed: 22542181] [MGI Ref ID J:188387]
Genetic Resource Sciences at The Jackson Laboratory. 2012. Expression/Specificity Patterns of Cre Alleles, 2011 MGI Direct Data Submission :. [MGI Ref ID J:184578]
Genetic Resource Sciences at The Jackson Laboratory. 2008. Expression/Specificity patterns of Cre transgenes MGI Direct Data Submission :. [MGI Ref ID J:137887]
Gorski JA; Balogh SA; Wehner JM; Jones KR. 2003. Learning deficits in forebrain-restricted brain-derived neurotrophic factor mutant mice. Neuroscience 121(2):341-54. [PubMed: 14521993] [MGI Ref ID J:100000]
Gorski JA; Zeiler SR; Tamowski S; Jones KR. 2003. Brain-derived neurotrophic factor is required for the maintenance of cortical dendrites. J Neurosci 23(17):6856-65. [PubMed: 12890780] [MGI Ref ID J:84683]
Griveau A; Borello U; Causeret F; Tissir F; Boggetto N; Karaz S; Pierani A. 2010. A novel role for Dbx1-derived Cajal-Retzius cells in early regionalization of the cerebral cortical neuroepithelium. PLoS Biol 8(7):e1000440. [PubMed: 20668538] [MGI Ref ID J:165303]
Harrison SJ; Nishinakamura R; Jones KR; Monaghan AP. 2012. Sall1 regulates cortical neurogenesis and laminar fate specification in mice: implications for neural abnormalities in Townes-Brocks syndrome. Dis Model Mech 5(3):351-65. [PubMed: 22228756] [MGI Ref ID J:179804]
Harwell CC; Parker PR; Gee SM; Okada A; McConnell SK; Kreitzer AC; Kriegstein AR. 2012. Sonic hedgehog expression in corticofugal projection neurons directs cortical microcircuit formation. Neuron 73(6):1116-26. [PubMed: 22445340] [MGI Ref ID J:183457]
He H; Mahnke AH; Doyle S; Fan N; Wang CC; Hall BJ; Tang YP; Inglis FM; Chen C; Erickson JD. 2012. Neurodevelopmental role for VGLUT2 in pyramidal neuron plasticity, dendritic refinement, and in spatial learning. J Neurosci 32(45):15886-901. [PubMed: 23136427] [MGI Ref ID J:192240]
Hippenmeyer S; Youn YH; Moon HM; Miyamichi K; Zong H; Wynshaw-Boris A; Luo L. 2010. Genetic mosaic dissection of lis1 and ndel1 in neuronal migration. Neuron 68(4):695-709. [PubMed: 21092859] [MGI Ref ID J:166047]
Hu H; Li J; Gagen CS; Gray NW; Zhang Z; Qi Y; Zhang P. 2011. Conditional knockout of protein O-mannosyltransferase 2 reveals tissue-specific roles of O-mannosyl glycosylation in brain development. J Comp Neurol 519(7):1320-37. [PubMed: 21452199] [MGI Ref ID J:179356]
Huang Z; Shimazu K; Woo NH; Zang K; Muller U; Lu B; Reichardt LF. 2006. Distinct roles of the beta 1-class integrins at the developing and the mature hippocampal excitatory synapse. J Neurosci 26(43):11208-19. [PubMed: 17065460] [MGI Ref ID J:114964]
Imayoshi I; Hirano K; Sakamoto M; Miyoshi G; Imura T; Kitano S; Miyachi H; Kageyama R. 2012. A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination. Neurosci Res 73(1):85-91. [PubMed: 22343123] [MGI Ref ID J:182745]
Ivanova E; Hwang GS; Pan ZH. 2010. Characterization of transgenic mouse lines expressing Cre recombinase in the retina. Neuroscience 165(1):233-43. [PubMed: 19837136] [MGI Ref ID J:158209]
Judson MC; Bergman MY; Campbell DB; Eagleson KL; Levitt P. 2009. Dynamic gene and protein expression patterns of the autism-associated met receptor tyrosine kinase in the developing mouse forebrain. J Comp Neurol 513(5):511-31. [PubMed: 19226509] [MGI Ref ID J:172491]
Jung HJ; Coffinier C; Choe Y; Beigneux AP; Davies BS; Yang SH; Barnes RH 2nd; Hong J; Sun T; Pleasure SJ; Young SG; Fong LG. 2012. Regulation of prelamin A but not lamin C by miR-9, a brain-specific microRNA. Proc Natl Acad Sci U S A 109(7):E423-31. [PubMed: 22308344] [MGI Ref ID J:182630]
Karalay O; Doberauer K; Vadodaria KC; Knobloch M; Berti L; Miquelajauregui A; Schwark M; Jagasia R; Taketo MM; Tarabykin V; Lie DC; Jessberger S. 2011. Prospero-related homeobox 1 gene (Prox1) is regulated by canonical Wnt signaling and has a stage-specific role in adult hippocampal neurogenesis. Proc Natl Acad Sci U S A 108(14):5807-12. [PubMed: 21436036] [MGI Ref ID J:171216]
Kawase-Koga Y; Low R; Otaegi G; Pollock A; Deng H; Eisenhaber F; Maurer-Stroh S; Sun T. 2010. RNAase-III enzyme Dicer maintains signaling pathways for differentiation and survival in mouse cortical neural stem cells. J Cell Sci 123(Pt 4):586-94. [PubMed: 20103535] [MGI Ref ID J:158531]
Kawase-Koga Y; Otaegi G; Sun T. 2009. Different timings of Dicer deletion affect neurogenesis and gliogenesis in the developing mouse central nervous system. Dev Dyn 238(11):2800-12. [PubMed: 19806666] [MGI Ref ID J:153666]
Kim S; Lehtinen MK; Sessa A; Zappaterra MW; Cho SH; Gonzalez D; Boggan B; Austin CA; Wijnholds J; Gambello MJ; Malicki J; LaMantia AS; Broccoli V; Walsh CA. 2010. The apical complex couples cell fate and cell survival to cerebral cortical development. Neuron 66(1):69-84. [PubMed: 20399730] [MGI Ref ID J:160624]
Kohwi M; Petryniak MA; Long JE; Ekker M; Obata K; Yanagawa Y; Rubenstein JL; Alvarez-Buylla A. 2007. A subpopulation of olfactory bulb GABAergic interneurons is derived from Emx1- and Dlx5/6-expressing progenitors. J Neurosci 27(26):6878-91. [PubMed: 17596436] [MGI Ref ID J:160206]
Kroll TT; O'Leary DD. 2005. Ventralized dorsal telencephalic progenitors in Pax6 mutant mice generate GABA interneurons of a lateral ganglionic eminence fate. Proc Natl Acad Sci U S A 102(20):7374-9. [PubMed: 15878992] [MGI Ref ID J:99239]
Kwon HJ; Ma S; Huang Z. 2011. Radial glia regulate Cajal-Retzius cell positioning in the early embryonic cerebral cortex. Dev Biol 351(1):25-34. [PubMed: 21185282] [MGI Ref ID J:170581]
Lee Y; Katyal S; Downing SM; Zhao J; Russell HR; McKinnon PJ. 2012. Neurogenesis requires TopBP1 to prevent catastrophic replicative DNA damage in early progenitors. Nat Neurosci 15(6):819-26. [PubMed: 22522401] [MGI Ref ID J:191367]
Lee Y; Shull ER; Frappart PO; Katyal S; Enriquez-Rios V; Zhao J; Russell HR; Brown EJ; McKinnon PJ. 2012. ATR maintains select progenitors during nervous system development. EMBO J 31(5):1177-89. [PubMed: 22266795] [MGI Ref ID J:181920]
Li G; Kataoka H; Coughlin SR; Pleasure SJ. 2009. Identification of a transient subpial neurogenic zone in the developing dentate gyrus and its regulation by Cxcl12 and reelin signaling. Development 136(2):327-35. [PubMed: 19103804] [MGI Ref ID J:143512]
Li HS; Wang D; Shen Q; Schonemann MD; Gorski JA; Jones KR; Temple S; Jan LY; Jan YN. 2003. Inactivation of Numb and Numblike in embryonic dorsal forebrain impairs neurogenesis and disrupts cortical morphogenesis. Neuron 40(6):1105-18. [PubMed: 14687546] [MGI Ref ID J:87149]
Li J; Yu M; Feng G; Hu H; Li X. 2011. Breaches of the pial basement membrane are associated with defective dentate gyrus development in mouse models of congenital muscular dystrophies. Neurosci Lett 505(1):19-24. [PubMed: 21970971] [MGI Ref ID J:178536]
Li Q; Bian S; Hong J; Kawase-Koga Y; Zhu E; Zheng Y; Yang L; Sun T. 2011. Timing specific requirement of microRNA function is essential for embryonic and postnatal hippocampal development. PLoS One 6(10):e26000. [PubMed: 21991391] [MGI Ref ID J:178118]
Liang H; Hippenmeyer S; Ghashghaei HT. 2013. A Nestin-cre ttransgenic mouse is insufficient for recombination in early embryonic neural progenitors Open Biol 1:1200-1203. [MGI Ref ID J:192871]
Liang H; Xiao G; Yin H; Hippenmeyer S; Horowitz JM; Ghashghaei HT. 2013. Neural development is dependent on the function of specificity protein 2 in cell cycle progression. Development 140(3):552-61. [PubMed: 23293287] [MGI Ref ID J:194076]
Livet J; Weissman TA; Kang H; Draft RW; Lu J; Bennis RA; Sanes JR; Lichtman JW. 2007. Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature 450(7166):56-62. [PubMed: 17972876] [MGI Ref ID J:125961]
Louvi A; Chen L; Two AM; Zhang H; Min W; Gunel M. 2011. Loss of cerebral cavernous malformation 3 (Ccm3) in neuroglia leads to CCM and vascular pathology. Proc Natl Acad Sci U S A 108(9):3737-42. [PubMed: 21321212] [MGI Ref ID J:170480]
Ma S; Kwon HJ; Johng H; Zang K; Huang Z. 2013. Radial glial neural progenitors regulate nascent brain vascular network stabilization via inhibition of Wnt signaling. PLoS Biol 11(1):e1001469. [PubMed: 23349620] [MGI Ref ID J:194480]
Madisen L; Mao T; Koch H; Zhuo JM; Berenyi A; Fujisawa S; Hsu YW; Garcia AJ 3rd; Gu X; Zanella S; Kidney J; Gu H; Mao Y; Hooks BM; Boyden ES; Buzsaki G; Ramirez JM; Jones AR; Svoboda K; Han X; Turner EE; Zeng H. 2012. A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing. Nat Neurosci 15(5):793-802. [PubMed: 22446880] [MGI Ref ID J:182204]
Madisen L; Zwingman TA; Sunkin SM; Oh SW; Zariwala HA; Gu H; Ng LL; Palmiter RD; Hawrylycz MJ; Jones AR; Lein ES; Zeng H. 2010. A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Nat Neurosci 13(1):133-40. [PubMed: 20023653] [MGI Ref ID J:155793]
Magri L; Cambiaghi M; Cominelli M; Alfaro-Cervello C; Cursi M; Pala M; Bulfone A; Garcia-Verdugo JM; Leocani L; Minicucci F; Poliani PL; Galli R. 2011. Sustained Activation of mTOR Pathway in Embryonic Neural Stem Cells Leads to Development of Tuberous Sclerosis Complex-Associated Lesions. Cell Stem Cell 9(5):447-62. [PubMed: 22056141] [MGI Ref ID J:177840]
Miquelajauregui A; Van de Putte T; Polyakov A; Nityanandam A; Boppana S; Seuntjens E; Karabinos A; Higashi Y; Huylebroeck D; Tarabykin V. 2007. Smad-interacting protein-1 (Zfhx1b) acts upstream of Wnt signaling in the mouse hippocampus and controls its formation. Proc Natl Acad Sci U S A 104(31):12919-24. [PubMed: 17644613] [MGI Ref ID J:123406]
Niewmierzycka A; Mills J; St-Arnaud R; Dedhar S; Reichardt LF. 2005. Integrin-linked kinase deletion from mouse cortex results in cortical lamination defects resembling cobblestone lissencephaly. J Neurosci 25(30):7022-31. [PubMed: 16049178] [MGI Ref ID J:100025]
Nityanandam A; Parthasarathy S; Tarabykin V. 2012. Postnatal subventricular zone of the neocortex contributes GFAP+ cells to the rostral migratory stream under the control of Sip1. Dev Biol 366(2):341-56. [PubMed: 22546691] [MGI Ref ID J:185418]
Okerlund ND; Kivimae S; Tong CK; Peng IF; Ullian EM; Cheyette BN. 2010. Dact1 is a postsynaptic protein required for dendrite, spine, and excitatory synapse development in the mouse forebrain. J Neurosci 30(12):4362-8. [PubMed: 20335472] [MGI Ref ID J:159217]
Pawlisz AS; Feng Y. 2011. Three-dimensional regulation of radial glial functions by Lis1-Nde1 and dystrophin glycoprotein complexes. PLoS Biol 9(10):e1001172. [PubMed: 22028625] [MGI Ref ID J:184033]
Pereira JD; Sansom SN; Smith J; Dobenecker MW; Tarakhovsky A; Livesey FJ. 2010. Ezh2, the histone methyltransferase of PRC2, regulates the balance between self-renewal and differentiation in the cerebral cortex. Proc Natl Acad Sci U S A 107(36):15957-62. [PubMed: 20798045] [MGI Ref ID J:164377]
Pinon MC; Tuoc TC; Ashery-Padan R; Molnar Z; Stoykova A. 2008. Altered molecular regionalization and normal thalamocortical connections in cortex-specific Pax6 knock-out mice. J Neurosci 28(35):8724-34. [PubMed: 18753373] [MGI Ref ID J:138792]
Pucilowska J; Puzerey PA; Karlo JC; Galan RF; Landreth GE. 2012. Disrupted ERK Signaling during Cortical Development Leads to Abnormal Progenitor Proliferation, Neuronal and Network Excitability and Behavior, Modeling Human Neuro-Cardio-Facial-Cutaneous and Related Syndromes. J Neurosci 32(25):8663-77. [PubMed: 22723706] [MGI Ref ID J:185659]
Qiu S; Anderson CT; Levitt P; Shepherd GM. 2011. Circuit-specific intracortical hyperconnectivity in mice with deletion of the autism-associated met receptor tyrosine kinase. J Neurosci 31(15):5855-64. [PubMed: 21490227] [MGI Ref ID J:170966]
Qiu Z; Cang Y; Goff SP. 2010. Abl family tyrosine kinases are essential for basement membrane integrity and cortical lamination in the cerebellum. J Neurosci 30(43):14430-9. [PubMed: 20980600] [MGI Ref ID J:166211]
Rasin MR; Gazula VR; Breunig JJ; Kwan KY; Johnson MB; Liu-Chen S; Li HS; Jan LY; Jan YN; Rakic P; Sestan N. 2007. Numb and Numbl are required for maintenance of cadherin-based adhesion and polarity of neural progenitors. Nat Neurosci 10(7):819-27. [PubMed: 17589506] [MGI Ref ID J:124218]
Ravni A; Qu Y; Goffinet AM; Tissir F. 2009. Planar cell polarity cadherin Celsr1 regulates skin hair patterning in the mouse. J Invest Dermatol 129(10):2507-9. [PubMed: 19357712] [MGI Ref ID J:157059]
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Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as homozygotes. Mating System Homozygote x Homozygote (Female x Male) 02-NOV-06 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Homozygous for Emx1tm1(cre)Krj
Price per Pair (US dollars $) Pair Genotype $464.00 Homozygous for Emx1tm1(cre)Krj x Homozygous for Emx1tm1(cre)Krj Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Homozygous for Emx1tm1(cre)Krj
Price per Pair (US dollars $) Pair Genotype $603.20 Homozygous for Emx1tm1(cre)Krj x Homozygous for Emx1tm1(cre)Krj Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use
Terms of Use
General Terms and Conditions
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.