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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names B6.Cg-Emx1tm1(cre)Krj/J (Changed: 27-MAR-08 ) B6.129-Emx1tm1(cre)Krj/J (Changed: 27-MAR-08 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 02-NOV-06 Species laboratory mouse Generation N12+F10 (18-NOV-09) Donating Investigator Kevin Jones, University of Colorado- Boulder Description
Mice homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This strain expresses Cre recombinase from the endogenous Emx1 locus. Western blot analysis of cortical brain tissue does not detect reduced endogenous gene product (protein). When crossed with a strain containing a loxP-site flanked sequence, Cre-mediated recombination results in tissue-specific deletion of the flanked sequence. Recombination occurs in approximately 88% of the neurons of the neocortex and hippocampus, and in the glial cells of the pallium, mimicking the pattern of expression of the endogenous gene. Further, the donating investigator reports that Cre recombinase is also expressed in a subset of male germline cells, thus some offspring from a cre; floxed male will have the floxed allele recombined in all cells. This mutant mouse strain represents a model that may be useful in studies of forebrain development and function.Development
A targeting vector containing an internal ribosome entry site (IRES) sequence, Cre coding sequence, a polyadenlyation signal and a FRT site flanked PGK-neomycin cassette was inserted 120bp 3' from the stop codon, in the 3' untranslated region. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animal was crossed to a Flp recombinase expressing strain (FLP-4917) to remove the selection cassette. The mice were then backcrossed to C57BL/6 mice for 12 generations.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Emx1
003080 B6;129X1-Emx1tm1Jlr/J View Strains carrying other alleles of Emx1 (1 strain)
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Emx1tm1(cre)Krj/Emx1tm1(cre)Krj
involves: 129S2/SvPas * C57BL/6
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:78098)
- homozygous mice do not show any of the abnormal phenotypes shown for Emx1tm1Jlr homozygotes
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Neurodevelopmental Defects
Research Tools
Cre-lox System
Cre Recombinase Expression: Germline/Embryonic Expression
Developmental Biology Research
Cre-lox System
Neurobiology Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Emx1tm1(cre)Krj | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Kevin R Jones | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Emx1-IRES-Cre; Emx1IREScre; emx1-cre; | ||
| Mutation Made By | Kevin Jones, University of Colorado- Boulder | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Site of Expression | neurons of the neocortex and hippocampus | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Emx1, empty spiracles homolog 1 (Drosophila) | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | RGD1564002; | ||
| Driver Note | Emx1 | ||
| Molecular Note | An IRES-cre gene followed by an FRT flanked PGK-neomycin gene was inserted 120 bp 3' of the stop codon. The neomycin gene was later removed in vivo by crossing to an Flp recombinase expressing transgenic strain. The protein product of this gene was produced in normal quantities as confirmed by Western blot analysis. Cre recombinase expression was characterized by crossing to a Rosa26 strain. [MGI Ref ID J:78098] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Emx1tm1(cre)Krj, Standard PCR
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Gorski JA; Talley T; Qiu M; Puelles L; Rubenstein JL; Jones KR. 2002. Cortical excitatory neurons and glia, but not GABAergic neurons, are produced in the Emx1-expressing lineage. J Neurosci 22(15):6309-14. [PubMed: 12151506] [MGI Ref ID J:78098]
Additional References
Emx1tm1(cre)Krj relatedArmentano M; Chou SJ; Tomassy GS; Leingartner A; O'Leary DD; Studer M. 2007. COUP-TFI regulates the balance of cortical patterning between frontal/motor and sensory areas. Nat Neurosci 10(10):1277-86. [PubMed: 17828260] [MGI Ref ID J:127387]
Baquet ZC; Gorski JA; Jones KR. 2004. Early striatal dendrite deficits followed by neuron loss with advanced age in the absence of anterograde cortical brain-derived neurotrophic factor. J Neurosci 24(17):4250-8. [PubMed: 15115821] [MGI Ref ID J:96903]
Barros CS; Calabrese B; Chamero P; Roberts AJ; Korzus E; Lloyd K; Stowers L; Mayford M; Halpain S; Muller U. 2009. Impaired maturation of dendritic spines without disorganization of cortical cell layers in mice lacking NRG1/ErbB signaling in the central nervous system. Proc Natl Acad Sci U S A 106(11):4507-12. [PubMed: 19240213] [MGI Ref ID J:146779]
Beggs HE; Schahin-Reed D; Zang K; Goebbels S; Nave KA; Gorski J; Jones KR; Sretavan D; Reichardt LF. 2003. FAK deficiency in cells contributing to the basal lamina results in cortical abnormalities resembling congenital muscular dystrophies. Neuron 40(3):501-14. [PubMed: 14642275] [MGI Ref ID J:86453]
Berger J; Berger S; Tuoc TC; D'Amelio M; Cecconi F; Gorski JA; Jones KR; Gruss P; Stoykova A. 2007. Conditional activation of Pax6 in the developing cortex of transgenic mice causes progenitor apoptosis. Development 134(7):1311-22. [PubMed: 17329367] [MGI Ref ID J:135032]
Bloom AJ; Miller BR; Sanes JR; Diantonio A. 2007. The requirement for Phr1 in CNS axon tract formation reveals the corticostriatal boundary as a choice point for cortical axons. Genes Dev 21(20):2593-606. [PubMed: 17901218] [MGI Ref ID J:125702]
Brinkmann BG; Agarwal A; Sereda MW; Garratt AN; Muller T; Wende H; Stassart RM; Nawaz S; Humml C; Velanac V; Radyushkin K; Goebbels S; Fischer TM; Franklin RJ; Lai C; Ehrenreich H; Birchmeier C; Schwab MH; Nave KA. 2008. Neuregulin-1/ErbB signaling serves distinct functions in myelination of the peripheral and central nervous system. Neuron 59(4):581-95. [PubMed: 18760695] [MGI Ref ID J:145464]
Depaepe V; Suarez-Gonzalez N; Dufour A; Passante L; Gorski JA; Jones KR; Ledent C; Vanderhaeghen P. 2005. Ephrin signalling controls brain size by regulating apoptosis of neural progenitors. Nature 435(7046):1244-50. [PubMed: 15902206] [MGI Ref ID J:99355]
Elia LP; Yamamoto M; Zang K; Reichardt LF. 2006. p120 catenin regulates dendritic spine and synapse development through Rho-family GTPases and cadherins. Neuron 51(1):43-56. [PubMed: 16815331] [MGI Ref ID J:110469]
Gorski JA; Balogh SA; Wehner JM; Jones KR. 2003. Learning deficits in forebrain-restricted brain-derived neurotrophic factor mutant mice. Neuroscience 121(2):341-54. [PubMed: 14521993] [MGI Ref ID J:100000]
Gorski JA; Zeiler SR; Tamowski S; Jones KR. 2003. Brain-derived neurotrophic factor is required for the maintenance of cortical dendrites. J Neurosci 23(17):6856-65. [PubMed: 12890780] [MGI Ref ID J:84683]
Huang Z; Shimazu K; Woo NH; Zang K; Muller U; Lu B; Reichardt LF. 2006. Distinct roles of the beta 1-class integrins at the developing and the mature hippocampal excitatory synapse. J Neurosci 26(43):11208-19. [PubMed: 17065460] [MGI Ref ID J:114964]
Kawase-Koga Y; Otaegi G; Sun T. 2009. Different timings of Dicer deletion affect neurogenesis and gliogenesis in the developing mouse central nervous system. Dev Dyn 238(11):2800-12. [PubMed: 19806666] [MGI Ref ID J:153666]
Kroll TT; O'Leary DD. 2005. Ventralized dorsal telencephalic progenitors in Pax6 mutant mice generate GABA interneurons of a lateral ganglionic eminence fate. Proc Natl Acad Sci U S A 102(20):7374-9. [PubMed: 15878992] [MGI Ref ID J:99239]
Li G; Kataoka H; Coughlin SR; Pleasure SJ. 2009. Identification of a transient subpial neurogenic zone in the developing dentate gyrus and its regulation by Cxcl12 and reelin signaling. Development 136(2):327-35. [PubMed: 19103804] [MGI Ref ID J:143512]
Li HS; Wang D; Shen Q; Schonemann MD; Gorski JA; Jones KR; Temple S; Jan LY; Jan YN. 2003. Inactivation of Numb and Numblike in embryonic dorsal forebrain impairs neurogenesis and disrupts cortical morphogenesis. Neuron 40(6):1105-18. [PubMed: 14687546] [MGI Ref ID J:87149]
Livet J; Weissman TA; Kang H; Draft RW; Lu J; Bennis RA; Sanes JR; Lichtman JW. 2007. Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous system. Nature 450(7166):56-62. [PubMed: 17972876] [MGI Ref ID J:125961]
Miquelajauregui A; Van de Putte T; Polyakov A; Nityanandam A; Boppana S; Seuntjens E; Karabinos A; Higashi Y; Huylebroeck D; Tarabykin V. 2007. Smad-interacting protein-1 (Zfhx1b) acts upstream of Wnt signaling in the mouse hippocampus and controls its formation. Proc Natl Acad Sci U S A 104(31):12919-24. [PubMed: 17644613] [MGI Ref ID J:123406]
Niewmierzycka A; Mills J; St-Arnaud R; Dedhar S; Reichardt LF. 2005. Integrin-linked kinase deletion from mouse cortex results in cortical lamination defects resembling cobblestone lissencephaly. J Neurosci 25(30):7022-31. [PubMed: 16049178] [MGI Ref ID J:100025]
Pinon MC; Tuoc TC; Ashery-Padan R; Molnar Z; Stoykova A. 2008. Altered molecular regionalization and normal thalamocortical connections in cortex-specific Pax6 knock-out mice. J Neurosci 28(35):8724-34. [PubMed: 18753373] [MGI Ref ID J:138792]
Rasin MR; Gazula VR; Breunig JJ; Kwan KY; Johnson MB; Liu-Chen S; Li HS; Jan LY; Jan YN; Rakic P; Sestan N. 2007. Numb and Numbl are required for maintenance of cadherin-based adhesion and polarity of neural progenitors. Nat Neurosci 10(7):819-27. [PubMed: 17589506] [MGI Ref ID J:124218]
Strand AD; Baquet ZC; Aragaki AK; Holmans P; Yang L; Cleren C; Beal MF; Jones L; Kooperberg C; Olson JM; Jones KR. 2007. Expression profiling of Huntington's disease models suggests that brain-derived neurotrophic factor depletion plays a major role in striatal degeneration. J Neurosci 27(43):11758-68. [PubMed: 17959817] [MGI Ref ID J:127037]
Tuoc TC; Radyushkin K; Tonchev AB; Pinon MC; Ashery-Padan R; Molnar Z; Davidoff MS; Stoykova A. 2009. Selective cortical layering abnormalities and behavioral deficits in cortex-specific Pax6 knock-out mice. J Neurosci 29(26):8335-49. [PubMed: 19571125] [MGI Ref ID J:150808]
Willaime-Morawek S; Seaberg RM; Batista C; Labbe E; Attisano L; Gorski JA; Jones KR; Kam A; Morshead CM; van der Kooy D. 2006. Embryonic cortical neural stem cells migrate ventrally and persist as postnatal striatal stem cells. J Cell Biol 175(1):159-68. [PubMed: 17030986] [MGI Ref ID J:114513]
Yoshida M; Assimacopoulos S; Jones KR; Grove EA. 2006. Massive loss of Cajal-Retzius cells does not disrupt neocortical layer order. Development 133(3):537-45. [PubMed: 16410414] [MGI Ref ID J:104373]
Zhou L; Bar I; Achouri Y; Campbell K; De Backer O; Hebert JM; Jones K; Kessaris N; de Rouvroit CL; O'Leary D; Richardson WD; Goffinet AM; Tissir F. 2008. Early forebrain wiring: genetic dissection using conditional Celsr3 mutant mice. Science 320(5878):946-9. [PubMed: 18487195] [MGI Ref ID J:134879]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as homozygotes. Mating System Homozygote x Homozygote (Female x Male) 02-NOV-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $160.30 Female or Male Homozygous for Emx1tm1(cre)Krj
Pairs /Price (US dollars $) Pair Genotype $320.60 Homozygous for Emx1tm1(cre)Krj x Homozygous for Emx1tm1(cre)Krj
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $208.40 Female or Male Homozygous for Emx1tm1(cre)Krj
Pairs /Price (US dollars $) Pair Genotype $416.80 Homozygous for Emx1tm1(cre)Krj x Homozygous for Emx1tm1(cre)Krj
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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