Strain Name: |
B6.129S2-Emx1tm1(cre)Krj/J |
|---|---|
Stock Number: |
005628 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6.Cg-Emx1tm1(cre)Krj/J (Changed: 27-MAR-08
) |
|
B6.129-Emx1tm1(cre)Krj/J (Changed: 27-MAR-08
) | |
| Genes & Alleles | Emx1; Emx1tm1(cre)Krj; cre; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Kevin Jones, University of Colorado- Boulder Generation N12+F5 (20-DEC-07) Strain Description
Mice homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. This strain expresses Cre recombinase from the endogenous Emx1 locus. Western blot analysis of cortical brain tissue does not detect reduced endogenous gene product (protein). When crossed with a strain containing a loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs in approximately 88% of the neurons of the neocortex and hippocampus, and in the glial cells of the pallium, mimicking the pattern of expression of the endogenous gene. This mutant mouse strain represents a model that may be useful in studies of forebrain development and function.Strain Development
A targeting vector containing an internal ribosome entry site (IRES) sequence, Cre coding sequence, a polyadenlyation signal and a FRT site flanked PGK-neomycin cassette was inserted 120bp 3' from the stop codon, in the 3' untranslated region. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animal was crossed to a Flp recombinase expressing strain (FLP-4917) to remove the selection cassette. The mice were then backcrossed to C57BL/6 mice for 12 generations.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Emx1tm1(cre)Krj | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Kevin R Jones | ||
| Common Name(s) | Emx1-IRES-Cre; Emx1IREScre; emx1-cre; | ||
| Mutation Made By | Kevin Jones, University of Colorado- Boulder | ||
| Strain of Origin | 129S2/SvPas | ||
| ES Cell Line Name | D3 | ||
| ES Cell Line Strain | 129S2/SvPas | ||
| Site of Expression | neurons of the neocortex and hippocampus | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Emx1, empty spiracles homolog 1 (Drosophila) | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | RGD1564002; | ||
| Molecular Note | An IRES-cre gene followed by an FRT flanked PGK-neomycin gene was inserted 120 bp 3' of the stop codon. The neomycin gene was later removed in vivo by crossing to an Flp recombinase expressing transgenic strain. The protein product of this gene was produced in normal quantities as confirmed by Western blot analysis. Cre recombinase expression was characterized by crossing to a Rosa26 strain. [MGI Ref ID J:78098] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Emx1tm1(cre)Krj
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as homozygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Emx1
003080 B6;129X1-Emx1tm1Jlr/J View Strains carrying other alleles of Emx1 (1 strain)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System (Cre Recombinase expression in neural tissue)
Neurodevelopmental Defects
Research Tools
Cre-lox System (Cre-Recombinase Expression: Germline/Embryonic Expression)
Developmental Biology Research (Cre-lox System)
Neurobiology Research
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesGorski JA; Talley T; Qiu M; Puelles L; Rubenstein JL; Jones KR. 2002. Cortical excitatory neurons and glia, but not GABAergic neurons, are produced in the Emx1-expressing lineage. J Neurosci 22(15):6309-14. [PubMed: 12151506] [MGI Ref ID J:78098]
Price and Supply Information
| Strain Name: | B6.129S2-Emx1tm1(cre)Krj/J |
| Stock Number: | 005628 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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