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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation ?+N1p (05-FEB-06) Donating Investigator Joshua Sanes, Harvard University Description
These transgenic mice conditionally express Enhanced Yellow Fluorescent Protein (EYFP) under the direction of the mouse thymus cell antigen 1, theta, promoter. Expression of the EYFP gene is blocked by a loxP-flanked STOP fragment placed between the promoter and EYFP gene. Cre-mediated excision of the STOP cassette results in expression of EYFP in motor neurons. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.For example, when crossed to a strain with widespread expression of Cre recombinase (see Stock No. 003376), this mutant mouse strain may be useful in studies of synaptic function.
Development
A transgenic construct containing an Enhanced Yellow Fluorescent Protein gene under the control of the mouse thymus cell antigen 1, theta, promoter, and with a loxP flanked STOP cassette inserted between the promoter and EYFP gene, was injected into fertilized B6CBAF1 mouse eggs. The resulting transgenic animals of founder line 15 were backcrossed to C57BL/6 mice for 6 generations.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
005483 129-Tg(CAG-EYFP)7AC5Nagy/J 006148 B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J 008299 B6.Cg-Tg(NEFL-EYFP/Nefh)40Gsn/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 007612 B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J 007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J 003709 B6.Cg-Tg(Thy1-YFP)16Jrs/J 005627 B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J 003782 B6.Cg-Tg(Thy1-YFPH)2Jrs/J 007606 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J 008636 B6;C-Tg(Prnp-APP695*/EYFP)49Gsn/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 005620 B6;D2-Tg(S100B-EYFP)1Wjt/J 007610 B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 007880 B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J 006618 C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J 006362 C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J 007857 C57BL/6J-Tg(Eno2-YFP/Cox8a)YRwb/J 007860 C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J 009422 NOD.Cg-Tg(Itgax-EYFP)1Mnz/QtngJ 005130 STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
Tg(Thy1-EYFP)15Jrs/0
B6.Cg-Tg(Thy1-EYFP)15Jrs
- no phenotypic analysis
- *normal* no phenotypic analysis (MGI Ref ID J:89596)
- no analysis provided
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Tg(ACTB-cre)2Mrt/0 Tg(Thy1-EYFP)15Jrs/0
involves: C57BL/6 * CBA * FVB/N
- nervous system phenotype
- *normal* nervous system phenotype (MGI Ref ID J:89596)
- in embryos, all motor axons are labeled with YFP
This mouse can be used to support research in many areas including:
YFP relatedNeurobiology Research
Cre-lox System
loxP-flanked Sequences
Fluorescent protein expression in neural tissue
Research Tools
Cre-lox System
loxP-flanked Sequences: Test/Reporter
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Thy1-EYFP)15Jrs | ||
|---|---|---|---|
| Allele Name | transgene insertion 15, Joshua R Sanes | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Thy1-STOP-YFP; | ||
| Mutation Made By | Joshua Sanes, Harvard University | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Site of Expression | when crossed to the appropriate Cre recombinase-expressing strain, expression of Enhanced Yellow Fluorescent Protein is observed in motor neurons | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| Molecular Note | The transgenic construct contains an Enhanced Yellow Fluorescent Protein gene under the control of the mouse thymus cell antigen 1, theta, promoter, and with a loxP flanked STOP cassette inserted between the promoter and EYFP gene. Line 15 was established. [MGI Ref ID J:89596] | ||
| Gene Symbol and Name | Tg(Thy1-EYFP)15Jrs, transgene insertion 15, Joshua R Sanes | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Thy1-STOP-YFP; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Standard PCR
Fluorescent Proteins -- Generic GFP, QPCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Buffelli M; Burgess RW; Feng G; Lobe CG; Lichtman JW; Sanes JR. 2003. Genetic evidence that relative synaptic efficacy biases the outcome of synaptic competition. Nature 424(6947):430-4. [PubMed: 12879071] [MGI Ref ID J:89596]
Additional References
Bareyre FM; Kerschensteiner M; Misgeld T; Sanes JR. 2005. Transgenic labeling of the corticospinal tract for monitoring axonal responses to spinal cord injury. Nat Med 11(12):1355-60. [PubMed: 16286922] [MGI Ref ID J:123607]
Tg(Thy1-EYFP)15Jrs relatedFox MA; Sanes JR; Borza DB; Eswarakumar VP; Fassler R; Hudson BG; John SW; Ninomiya Y; Pedchenko V; Pfaff SL; Rheault MN; Sado Y; Segal Y; Werle MJ; Umemori H. 2007. Distinct target-derived signals organize formation, maturation, and maintenance of motor nerve terminals. Cell 129(1):179-93. [PubMed: 17418794] [MGI Ref ID J:126496]
Kim IJ; Zhang Y; Yamagata M; Meister M; Sanes JR. 2008. Molecular identification of a retinal cell type that responds to upward motion. Nature 452(7186):478-82. [PubMed: 18368118] [MGI Ref ID J:134167]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, transgenic mice are bred together.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 Cryopreserved Embryos $1600.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 Cryopreserved Embryos $2080.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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JAX® Mice, Products & Services Conditions of Use
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