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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Donating Investigator Joshua R Sanes, Harvard University Description
These transgenic mice conditionally express Enhanced Yellow Fluorescent Protein (EYFP) under the direction of the mouse thymus cell antigen 1, theta, promoter. Expression of the EYFP gene is blocked by a loxP-flanked STOP fragment placed between the promoter and EYFP gene. Cre-mediated excision of the STOP cassette results in expression of EYFP in motor neurons. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.For example, when crossed to a strain with widespread expression of Cre recombinase (see Stock No. 003376), this mutant mouse strain may be useful in studies of synaptic function.
Development
A transgenic construct containing an Enhanced Yellow Fluorescent Protein gene under the control of the mouse thymus cell antigen 1, theta, promoter, and with a loxP flanked STOP cassette inserted between the promoter and EYFP gene, was injected into fertilized B6CBAF1 mouse eggs. The resulting transgenic animals of founder line 15 were backcrossed to C57BL/6 mice for 6 generations.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (358 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Phenotype
Phenotype Information
assigned by genotype
Tg(Thy1-EYFP)15Jrs/0
B6.Cg-Tg(Thy1-EYFP)15Jrs
- no phenotypic analysis
- *normal* no phenotypic analysis
- no analysis provided (MGI Ref ID J:89596)
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Tg(ACTB-cre)2Mrt/0 Tg(Thy1-EYFP)15Jrs/0
involves: C57BL/6 * CBA * FVB/N
- nervous system phenotype
- *normal* nervous system phenotype
- in embryos, all motor axons are labeled with YFP (MGI Ref ID J:89596)
This mouse can be used to support research in many areas including:
YFP relatedNeurobiology Research
Cre-lox System
loxP-flanked Sequences
Fluorescent protein expression in neural tissue
Research Tools
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: neurons
Neurobiology Research
cell marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Thy1-EYFP)15Jrs | ||
|---|---|---|---|
| Allele Name | transgene insertion 15, Joshua R Sanes | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | TSY; Thy1-EYGP; Thy1-STOP-YFP; Thy1-lox-STOP-lox-EYFP; ThyStopY; | ||
| Mutation Made By | Joshua Sanes, Harvard University | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Site of Expression | when crossed to the appropriate Cre recombinase-expressing strain, expression of Enhanced Yellow Fluorescent Protein is observed in motor neurons | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| Molecular Note | The transgenic construct contains an Enhanced Yellow Fluorescent Protein gene under the control of the mouse thymus cell antigen 1, theta, promoter, and with a loxP flanked STOP cassette inserted between the promoter and EYFP gene. Line 15 was established. [MGI Ref ID J:89596] | ||
| Gene Symbol and Name | Tg(Thy1-EYFP)15Jrs, transgene insertion 15, Joshua R Sanes | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Thy1-STOP-YFP; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Fluorescent Proteins -- Generic GFP, QPCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Buffelli M; Burgess RW; Feng G; Lobe CG; Lichtman JW; Sanes JR. 2003. Genetic evidence that relative synaptic efficacy biases the outcome of synaptic competition. Nature 424(6947):430-4. [PubMed: 12879071] [MGI Ref ID J:89596]
Additional References
Bareyre FM; Kerschensteiner M; Misgeld T; Sanes JR. 2005. Transgenic labeling of the corticospinal tract for monitoring axonal responses to spinal cord injury. Nat Med 11(12):1355-60. [PubMed: 16286922] [MGI Ref ID J:123607]
Tg(Thy1-EYFP)15Jrs relatedBareyre FM; Kerschensteiner M; Misgeld T; Sanes JR. 2005. Transgenic labeling of the corticospinal tract for monitoring axonal responses to spinal cord injury. Nat Med 11(12):1355-60. [PubMed: 16286922] [MGI Ref ID J:123607]
Benito-Gonzalez A; Alvarez FJ. 2012. Renshaw Cells and Ia Inhibitory Interneurons Are Generated at Different Times from p1 Progenitors and Differentiate Shortly after Exiting the Cell Cycle. J Neurosci 32(4):1156-70. [PubMed: 22279202] [MGI Ref ID J:180506]
Betley JN; Wright CV; Kawaguchi Y; Erdelyi F; Szabo G; Jessell TM; Kaltschmidt JA. 2009. Stringent specificity in the construction of a GABAergic presynaptic inhibitory circuit. Cell 139(1):161-74. [PubMed: 19804761] [MGI Ref ID J:157313]
Chakkalakal JV; Kuang S; Buffelli M; Lichtman JW; Sanes JR. 2012. Mouse transgenic lines that selectively label Type I, Type IIA, and Types IIX+B skeletal muscle fibers. Genesis 50(1):50-8. [PubMed: 21898764] [MGI Ref ID J:181057]
Chakkalakal JV; Nishimune H; Ruas JL; Spiegelman BM; Sanes JR. 2010. Retrograde influence of muscle fibers on their innervation revealed by a novel marker for slow motoneurons. Development 137(20):3489-99. [PubMed: 20843861] [MGI Ref ID J:165805]
Cheng X; Li Y; Huang Y; Feng X; Feng G; Xiong ZQ. 2011. Pulse labeling and long-term tracing of newborn neurons in the adult subgranular zone. Cell Res 21(2):338-49. [PubMed: 20938464] [MGI Ref ID J:187505]
De la Huerta I; Kim IJ; Voinescu PE; Sanes JR. 2012. Direction-selective retinal ganglion cells arise from molecularly specified multipotential progenitors. Proc Natl Acad Sci U S A 109(43):17663-8. [PubMed: 23045641] [MGI Ref ID J:190378]
English CN; Vigers AJ; Jones KR. 2012. Genetic evidence that brain-derived neurotrophic factor mediates competitive interactions between individual cortical neurons. Proc Natl Acad Sci U S A 109(47):19456-61. [PubMed: 23129644] [MGI Ref ID J:192261]
Fox MA; Sanes JR; Borza DB; Eswarakumar VP; Fassler R; Hudson BG; John SW; Ninomiya Y; Pedchenko V; Pfaff SL; Rheault MN; Sado Y; Segal Y; Werle MJ; Umemori H. 2007. Distinct target-derived signals organize formation, maturation, and maintenance of motor nerve terminals. Cell 129(1):179-93. [PubMed: 17418794] [MGI Ref ID J:126496]
Fuerst PG; Bruce F; Rounds RP; Erskine L; Burgess RW. 2012. Cell autonomy of DSCAM function in retinal development. Dev Biol 361(2):326-37. [PubMed: 22063212] [MGI Ref ID J:179393]
Kay JN; De la Huerta I; Kim IJ; Zhang Y; Yamagata M; Chu MW; Meister M; Sanes JR. 2011. Retinal ganglion cells with distinct directional preferences differ in molecular identity, structure, and central projections. J Neurosci 31(21):7753-62. [PubMed: 21613488] [MGI Ref ID J:190967]
Kay JN; Voinescu PE; Chu MW; Sanes JR. 2011. Neurod6 expression defines new retinal amacrine cell subtypes and regulates their fate. Nat Neurosci 14(8):965-72. [PubMed: 21743471] [MGI Ref ID J:176166]
Kim IJ; Zhang Y; Meister M; Sanes JR. 2010. Laminar restriction of retinal ganglion cell dendrites and axons: subtype-specific developmental patterns revealed with transgenic markers. J Neurosci 30(4):1452-62. [PubMed: 20107072] [MGI Ref ID J:157687]
Kim IJ; Zhang Y; Yamagata M; Meister M; Sanes JR. 2008. Molecular identification of a retinal cell type that responds to upward motion. Nature 452(7186):478-82. [PubMed: 18368118] [MGI Ref ID J:134167]
Shin JE; Cho Y; Beirowski B; Milbrandt J; Cavalli V; DiAntonio A. 2012. Dual leucine zipper kinase is required for retrograde injury signaling and axonal regeneration. Neuron 74(6):1015-22. [PubMed: 22726832] [MGI Ref ID J:188356]
Siegert S; Cabuy E; Scherf BG; Kohler H; Panda S; Le YZ; Fehling HJ; Gaidatzis D; Stadler MB; Roska B. 2012. Transcriptional code and disease map for adult retinal cell types. Nat Neurosci 15(3):487-95, S1-2. [PubMed: 22267162] [MGI Ref ID J:182307]
Zagoraiou L; Akay T; Martin JF; Brownstone RM; Jessell TM; Miles GB. 2009. A cluster of cholinergic premotor interneurons modulates mouse locomotor activity. Neuron 64(5):645-62. [PubMed: 20005822] [MGI Ref ID J:157468]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2450.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3185.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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For Licensing and Use Restrictions view the link(s) below:
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Contact information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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