Strain Name: |
B6.Cg-Tg(Thy1-EYFP)15Jrs/J |
|---|---|
Stock Number: |
005630 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Thy1; YFP; Tg(Thy1-EYFP)15Jrs; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Joshua Sanes, Harvard University Strain Description
These transgenic mice conditionally express Enhanced Yellow Fluorescent Protein (EYFP) under the direction of the mouse thymus cell antigen 1, theta, promoter. Expression of the EYFP gene is blocked by a loxP-flanked STOP fragment placed between the promoter and EYFP gene. Cre-mediated excision of the STOP cassette results in expression of EYFP in motor neurons. Mice hemizygous for the transgenic insert are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.For example, when crossed to a strain with widespread expression of Cre recombinase (see Stock No. 003376), this mutant mouse strain may be useful in studies of synaptic function.
Strain Development
A transgenic construct containing an Enhanced Yellow Fluorescent Protein gene under the control of the mouse thymus cell antigen 1, theta, promoter, and with a loxP flanked STOP cassette inserted between the promoter and EYFP gene, was injected into fertilized B6CBAF1 mouse eggs. The resulting transgenic animals of founder line 15 were backcrossed to C57BL/6 mice for 6 generations.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Thy1-EYFP)15Jrs | ||
|---|---|---|---|
| Allele Name | transgene insertion 15, Joshua R Sanes | ||
| Common Name(s) | Thy1-STOP-YFP; | ||
| Mutation Made By | Joshua Sanes, Harvard University | ||
| Strain of Origin | (C57BL/6 x CBA)F1 | ||
| Site of Expression | when crossed to the appropriate Cre recombinase-expressing strain, expression of Enhanced Yellow Fluorescent Protein is observed in motor neurons | ||
| Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish | ||
| Yellow Fluorescent Protein (YFP) is a derivative of Green Fluorescent Protein (GFP), a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance fluorescence intensity and shift the wavelength emitted when excited. When YFP is utilized in a transgenic construct, tissue expressing sufficient amounts of YFP will fluoresce yellowish-green when exposed to a 513 nm light source. | |||
| Promoter | Thy1, thymus cell antigen 1, theta, mouse, laboratory | ||
| Molecular Note | The transgenic construct contains an Enhanced Yellow Fluorescent Protein gene under the control of the mouse thymus cell antigen 1, theta, promoter, and with a loxP flanked STOP cassette inserted between the promoter and EYFP gene. Line 15 was established. [MGI Ref ID J:89596] | ||
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Fluorescent Proteins (Generic GFP)
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, transgenic mice are bred together. |
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Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (139 strains)
005483 129-Tg(CAG-EYFP)7AC5Nagy/J 006148 B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J 007901 B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J 007911 B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J 007921 B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J 007612 B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J 007615 B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J 003709 B6.Cg-Tg(Thy1-YFP)16Jrs/J 005627 B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J 003782 B6.Cg-Tg(Thy1-YFPH)2Jrs/J 007606 B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J 007910 B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J 005620 B6;D2-Tg(S100B-EYFP)1Wjt/J 007610 B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J 007880 B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J 006618 C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J 006362 C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J 005130 STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Fluorescent Proteins/lacZ Systems
Genetic Quality Control Annual Report
Research Applications
This mouse can be used to support research in many areas including:
YFP relatedNeurobiology Research
Cre-lox System (loxP-flanked Sequences)
Fluorescent protein expression in neural tissue
Research Tools
Cre-lox System (loxP-flanked Sequences: Test/Reporter)
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: neurons)
Neurobiology Research (cell marker)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesBuffelli M; Burgess RW; Feng G; Lobe CG; Lichtman JW; Sanes JR. 2003. Genetic evidence that relative synaptic efficacy biases the outcome of synaptic competition. Nature 424(6947):430-4. [PubMed: 12879071] [MGI Ref ID J:89596]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Thy1-EYFP)15Jrs/J |
| Stock Number: | 005630 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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