Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N18pN1 Generation Definitions   Donating Investigator Eva Eicher,   The Jackson Laboratory

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying   Zfpm2^tm1Sho allele

006838

D2.129S1(Cg)-Zfpm2tm1Sho/EiJ

View Strains carrying   Zfpm2^tm1Sho     (1 strain)

Strains carrying other alleles of Zfpm2

007266	B6Ei.129S1(Cg)-Zfpm2tm2Sho/EiJ
006768	D2.Cg-Tg(Myh6-Zfpm2)1Sho/EiJ

View Strains carrying other alleles of Zfpm2     (2 strains)

Additional Web Information

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI

- Model with phenotypic similarity to human disease where etiologies involve orthologs. Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).

Tetralogy of Fallot; TOF

- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested. Diaphragmatic Hernia 3   (ZFPM2)

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Zfpm2^tm1Sho/Zfpm2^tm1Sho

        involves: 129S1/Sv

• mortality/aging
• complete lethality throughout fetal growth and development
  • mutant embryos die at midgestation between E12.5 and E15.5; some are found dead by E13.5   (MGI Ref ID J:62879)
  • at E14.5, approximately 50% of mutant embryos are necrotic and undergoing resorption   (MGI Ref ID J:62879)
• cardiovascular system phenotype
• abnormal cardiac epithelial to mesenchymal transition
  • an early developmental block prevents epithelial-to-mesenchymal transition of "transforming" epicardial cells and subsequent formation of coronary vascular plexus within the subepicardial space   (MGI Ref ID J:62879)
• abnormal heart atrium morphology
  • at E13.5, mutant atria are thinner than normal and only a few trabeculae are observed   (MGI Ref ID J:62879)
• • atrial septal defect
    • atrial septal defects are detected at E13.5-E15.5   (MGI Ref ID J:62879)
  • dilated heart atrium
    • at E13.5, mutant atria are significantly dilated   (MGI Ref ID J:62879)
• abnormal heart shape
  • by 13.5, mutant hearts are rounder than normal, as sharp ventricular apicies are absent   (MGI Ref ID J:62879)
• absent coronary vessels
  • coronary vasculature is absent, despite formation of an intact epicardial layer and expression of epicardium-specific genes   (MGI Ref ID J:62879)
• common atrioventricular valve
  • a common atrioventricular valve is situated between the left and right ventricles   (MGI Ref ID J:62879)
• congestive heart failure
  • at E13.5, mutant embryos display an overall appearance consistent with heart failure   (MGI Ref ID J:62879)
• decreased heart rate
  • by E13.5, pulsation is weaker and slower, and blood cells remain within the heart even after contraction   (MGI Ref ID J:62879)
• heart right ventricle outflow tract stenosis
  • subpulmonic stenosis is detected at E13.5-E15.0   (MGI Ref ID J:62879)
• hemorrhage
  • at E13.5, mutant embryos often display peripheral hemorrhage   (MGI Ref ID J:62879)
  • however, no bleeding is noted around the heart   (MGI Ref ID J:62879)
• overriding aortic valve
  • an overriding aorta is detected at E13.5-E15.0   (MGI Ref ID J:62879)
• supravalvar pulmonary trunk stenosis
  • a severely obstructed pulmonary trunk (stenosis) is observed at E12.5   (MGI Ref ID J:62879)
• thin myocardium compact layer
  • at E13.5-E15.0, mutant embryos display thinning of the compact ventricular myocardium, similar to a "papyraceous phenotype"   (MGI Ref ID J:62879)
• thin ventricular wall   (MGI Ref ID J:62879)
• ventricular myocardium compact layer hypoplasia
  • a striking ventricular compact layer hypoplasia is observed   (MGI Ref ID J:62879)
• ventricular septal defect
  • a subaortic ventricular septal defect is noted at E13.5-E15.0, as part of a complete common atrioventricular canal   (MGI Ref ID J:62879)
• respiratory system phenotype
• abnormal lung development
  • branching in unaffected lobes appears delayed by 6 - 12 hours in homozygous lung culture explants; however, all mutant lungs do develop the normal branching pattern after 5 days in culture   (MGI Ref ID J:100119)
  • underdevelopment of lungs is observed at E13.5-E15, secondary to heart failure   (MGI Ref ID J:62879)
• absent right lung accessory lobe
  • the right lung accessory lobe fails to develop   (MGI Ref ID J:100119)
• pulmonary hypoplasia   (MGI Ref ID J:62879)
  • at E12, prior to onset of diaphragm function, mutant lungs are smaller and lack development of an accessory lobe   (MGI Ref ID J:100119)
• homeostasis/metabolism phenotype
• hydrops fetalis
  • at E13.5, mutant embryos are edematous   (MGI Ref ID J:62879)
• liver/biliary system phenotype
• small liver
  • a smaller liver size is observed at E13.5-E15.5, secondary to heart failure   (MGI Ref ID J:62879)
• integument phenotype
• pallor
  • at E13.5, mutant embryos are pale   (MGI Ref ID J:62879)

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Zfpm2tm1Sho
Allele Name		targeted mutation 1, Stuart Orkin
Allele Type		Targeted (knock-out)
Common Name(s)		FOG-2-; Fog2-;
Mutation Made By		Linda Washburn,   The Jackson Laboratory
Strain of Origin		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line Name		CJ7
ES Cell Line Strain		129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name		Zfpm2, zinc finger protein, multitype 2
Chromosome		15
Gene Common Name(s)		B330005D23Rik; DIH3; FOG-2; FOG2; RIKEN cDNA B330005D23 gene; ZC2HC11B; ZNF89B; hFOG-2;
Molecular Note		The gene was disrupted by replacement of sequences encoding all of the zinc finger motifs with a floxed neomycin-cytidine deaminase cassette. The floxed cassette was removed by crossing mutant animals to a cre deleter strain. Homozygous mutant embryos were detected by Southern blot analysis up to day E15.5. RNase protection assay showed an absence of Zfpm2 mRNA in hearts of E12.5 homozygous mutant embryos. [MGI Ref ID J:62879]

Genotyping

Genotyping Information

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Tevosian SG; Deconinck AE; Tanaka M; Schinke M; Litovsky SH; Izumo S; Fujiwara Y; Orkin SH. 2000. FOG-2, a cofactor for GATA transcription factors, is essential for heart morphogenesis and development of coronary vessels from epicardium. Cell 101(7):729-39. [PubMed: 10892744]  [MGI Ref ID J:62879]

Additional References

Zfpm2^tm1Sho related

Ackerman KG; Herron BJ; Vargas SO; Huang H; Tevosian SG; Kochilas L; Rao C; Pober BR; Babiuk RP; Epstein JA; Greer JJ; Beier DR. 2005. Fog2 is required for normal diaphragm and lung development in mice and humans. PLoS Genet 1(1):e10. [PubMed: 16103912]  [MGI Ref ID J:100119]

Bouma GJ; Washburn LL; Albrecht KH; Eicher EM. 2007. Correct dosage of Fog2 and Gata4 transcription factors is critical for fetal testis development in mice. Proc Natl Acad Sci U S A 104(38):14994-9. [PubMed: 17848526]  [MGI Ref ID J:125196]

Manuylov NL; Fujiwara Y; Adameyko II; Poulat F; Tevosian SG. 2007. The regulation of Sox9 gene expression by the GATA4/FOG2 transcriptional complex in dominant XX sex reversal mouse models. Dev Biol 307(2):356-67. [PubMed: 17540364]  [MGI Ref ID J:122944]

Manuylov NL; Smagulova FO; Leach L; Tevosian SG. 2008. Ovarian development in mice requires the GATA4-FOG2 transcription complex. Development 135(22):3731-43. [PubMed: 18927154]  [MGI Ref ID J:143586]

Manuylov NL; Smagulova FO; Tevosian SG. 2007. Fog2 excision in mice leads to premature mammary gland involution and reduced Esr1 gene expression Oncogene 26(36):5204-13. [PubMed: 17310981]  [MGI Ref ID J:120985]

Manuylov NL; Zhou B; Ma Q; Fox SC; Pu WT; Tevosian SG. 2011. Conditional ablation of Gata4 and Fog2 genes in mice reveals their distinct roles in mammalian sexual differentiation. Dev Biol 353(2):229-41. [PubMed: 21385577]  [MGI Ref ID J:173838]

Pazin DE; Albrecht KH. 2009. Developmental expression of Smoc1 and Smoc2 suggests potential roles in fetal gonad and reproductive tract differentiation. Dev Dyn 238(11):2877-90. [PubMed: 19842175]  [MGI Ref ID J:153731]

Smagulova FO; Manuylov NL; Leach LL; Tevosian SG. 2008. GATA4/FOG2 transcriptional complex regulates Lhx9 gene expression in murine heart development. BMC Dev Biol 8:67. [PubMed: 18577233]  [MGI Ref ID J:139183]

Tevosian SG; Albrecht KH; Crispino JD; Fujiwara Y; Eicher EM; Orkin SH. 2002. Gonadal differentiation, sex determination and normal Sry expression in mice require direct interaction between transcription partners GATA4 and FOG2. Development 129(19):4627-34. [PubMed: 12223418]  [MGI Ref ID J:78688]

Zhou B; Ma Q; Kong SW; Hu Y; Campbell PH; McGowan FX; Ackerman KG; Wu B; Zhou B; Tevosian SG; Pu WT. 2009. Fog2 is critical for cardiac function and maintenance of coronary vasculature in the adult mouse heart. J Clin Invest 119(6):1462-76. [PubMed: 19411759]  [MGI Ref ID J:150452]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls

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	000664 C57BL/6J
Considerations for Choosing Controls
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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