Strain Name: |
B6129-Tg(Myh6-cre/Esr1)1Jmk/J |
|---|---|
Stock Number: |
005650 |
Availability: | Repository- Live |
General Terms and Conditions |
| Former Name |
B6129-Tg(Myh6-cre/Esr1)1Jmk (Changed: 13-MAR-06
) |
| Genes & Alleles | Esr1; Myh6; cre; Tg(Myh6-cre/Esr1)1Jmk; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System Homozygote x Homozygote (Female x Male) Species laboratory mouse Donating Investigator Jeffery Molkentin, Children's Hospital Medical Center Generation +N1F5 (05-DEC-07) Strain Description
Mice that are homozygous for the transgene are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Cre expression in heart tissue is confirmed by western blot. Southern blot confirmed heart cell specificity compared to brain, kidney, lung, liver, and skeletal muscle. Insertion of this transgene and its protein show no changes in echocardiography, heart mass or pathology, or hypertrophy marker genes compared to nontransgenic littermates. Of note, this double fusion protein has substantially greater Cre recombinase activity with less promiscuity compared with a single CreMer fusion protein. Inducible expression of cre in cardiac cells makes this strain suitable for creating bitransgenic mice for use in studies of temporally regulated deletion of loxP-flanked targeted genes.Strain Development
A transgenic construct using the cardiac-specific murine alpha-myosin heavy chain promoter to direct expression of a double fusion protein was injected into FVB/N embryos. This double fusion protein, MerCreMer, has cre recombinase gene flanked on each end with a mutated murine estrogen receptor ligand binding domain (amino acids 281-599, G525R); thus rendering Cre expression tamoxifen inducible yet estrogen insensitive. These embryos were implanted into pseudopregnant FVB/N females. Founder animals were backcrossed to mice with a (B6 x 129/Sv)F1 genetic background for 15 generations and are now interbred.
Gene & Allele Details
| Allele Symbol | Tg(Myh6-cre/Esr1)1Jmk | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Jeffery D Molkentin | ||
| Common Name(s) | MCM; alpha-MHC-MerCreMer; | ||
| Mutation Made By | Jeffery Molkentin, Children's Hospital Medical Center | ||
| Strain of Origin | FVB | ||
| Site of Expression | tamoxifen inducible (yet estrogen insensitive) Cre recombinase protein fused to two mutant estrogen-receptor ligand-binding domains (MerCreMer); expression in developing and adult heart | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Expressed Gene | Esr1, estrogen receptor 1 (alpha), mouse, laboratory | ||
| Promoter | Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, murine, murine | ||
| Molecular Note | This transgene expresses a cre recombinase/ mutant estrogen receptor ligand binding domain fusion protein under the control of a mouse cardiac-specific alpha-myosin heavy chain promoter. Expression was detected in the juvenile and adult heart, but the protein is inactive until induced with tamoxifen. [J:82027] | ||
Control Information
| Control | ||
|---|---|---|
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice may be bred as homozygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Tg(Myh6-cre/Esr1)1Jmk allele
005657 B6.Cg-Tg(Myh6-cre/Esr1)1Jmk/J View Strains carrying Tg(Myh6-cre/Esr1)1Jmk (1 strain)
006403 129S.B6-Tg(KRT14-Esr1/HRAS)1Pkha/J 004744 B6.129P2-Esr1tm1Ksk/J 004682 B6.Cg-Tg(cre/Esr1)5Amc/J 006774 FVB-Tg(Col2a1-cre/ESR1)KA3Smac/J 006822 FVB-Tg(KRT14-MAP2K1/Esr1)12Pkha/J 008122 STOCK Tg(Ins2-cre/Esr1)1Dam/J 005107 STOCK Tg(KRT14-cre/Esr1)20Efu/J 008119 STOCK Tg(Neurog3-cre/Esr1)1Dam/J 004453 STOCK Tg(cre/Esr1)5Amc/J
Additional Web Information
Cre-lox or FLP-FRT Systems
Genetic Quality Control Annual Report
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cardiovascular Research (Cre-lox system)
Cre-lox-System (Cre-Recombinase Expression: Inducible)
Research Tools
Cre-lox-System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
References
Selected Reference(s)
Additional ReferencesSohal DS; Nghiem M; Crackower MA; Witt SA; Kimball TR; Tymitz KM; Penninger JM; Molkentin JD. 2001. Temporally regulated and tissue-specific gene manipulations in the adult and embryonic heart using a tamoxifen-inducible Cre protein. Circ Res 89(1):20-5. [PubMed: 11440973] [J:82027]
Price and Supply Information
| Strain Name: | B6129-Tg(Myh6-cre/Esr1)1Jmk/J |
| Stock Number: | 005650 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
*NO Shipping Destination selected!
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Notice Regarding cre/Esr1 (Tamoxifen Inducible Cre) Strains.
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form