Strain Name: |
C.Cg-Gata1tm6Sho/J |
|---|---|
Stock Number: |
005653 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | Gata1; Gata1tm6Sho; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Stuart Orkin, Harvard Medical School Generation N8+F5 (05-DEC-07) Strain Description
Homozygous females and hemizygous males for the targeted mutation are viable, fertile, and normal in size. This mutation results in the complete ablation of the eosinophil lineage, even under conditions that normally stimulate eosinophil development, without affecting the development of other GATA-1 dependent lineages (erythroid, megakaryocytic, and mast cells). Expression of the endogenous gene is observed in erythroid and bone marrow cells. This mutant may be useful for in vivo studies of eosinophil function and eosinophil-related pathologies, including asthma and pulmonary physiology.Strain Development
A targeting vector was designed to replace the double GATA-site 21 bp upstream of the first hematopoietic exon of the X-linked endogenous gene. This vector, containing a loxP-flanked phosphoglycerate kinase promoter driven neomycin resistance gene (PGK-neo), was electroporated into 129S1/Sv derived CJ-7 embryonic stem (ES) cells. Correctly targeted ES cells were then transiently transfected with a cre recombinase vector to remove the PGK-neo resulting in a mutant allele where the double GATA-site is replaced with a single loxP site. These cells were injected into C57BL/6 blastocysts and the resulting chimeric males were backcrossed to C57BL/6 females. Resultant heterozygotes were backcrossed to BALB/c for eight generations before being made homozygous/hemizygous.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Gata1tm6Sho | ||
|---|---|---|---|
| Allele Name | targeted mutation 6, Stuart Orkin | ||
| Common Name(s) | Gata1tm5Sho; deltadblGATA; | ||
| Mutation Made By | Stuart Orkin, Harvard Medical School | ||
| Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| ES Cell Line Name | CJ7 | ||
| ES Cell Line Strain | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | ||
| Gene Symbol and Name | Gata1, GATA binding protein 1 | ||
| Chromosome | X | ||
| Gene Common Name(s) | ERYF1; GF-1; GF1; Gata-1; Gf-1; NFE1; globin factor 1; | ||
| Molecular Note | A high-affinity, palindromic "double" GATA protein binding site in the Gata1 promoter presumed to mediate positive Gata1 autoregulation was replaced by a floxed Pgk-neo cassette; transient Cre recombinase expression in ES cells left a single loxP site flanked by two Not1 sites. The 21-bp deleted segment comprised nucleotides -691 through -671 upstream of the last nucleotide of the first hematopoietically expressed exon of Gata1. [MGI Ref ID J:88711] | ||
Control Information
| Control | ||
|---|---|---|
| 000651 BALB/cJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Gata1tm6Sho
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, this colony is maintained as homozygotes. |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying other alleles of Gata1
004655 STOCK Gata1tm2Sho/J View Strains carrying other alleles of Gata1 (1 strain)
Additional Web Information
Congenic Nomenclature
Animal Health Reports
Room Number AX12
Research Applications
This mouse can be used to support research in many areas including:
Developmental Biology Research
Lymphoid Tissue Defects (hematopoietic defects)
Hematological Research
Hematopoietic Defects
Immunology and Inflammation Research
Immunodeficiency (Asthma)
Inflammation (Asthma)
Lymphoid Tissue Defects (hematopoietic development)
References
Selected Reference(s)
Additional ReferencesYu C; Cantor AB; Yang H; Browne C; Wells RA; Fujiwara Y; Orkin SH. 2002. Targeted deletion of a high-affinity GATA-binding site in the GATA-1 promoter leads to selective loss of the eosinophil lineage in vivo. J Exp Med 195(11):1387-95. [PubMed: 12045237] [MGI Ref ID J:88711]
Price and Supply Information
| Strain Name: | C.Cg-Gata1tm6Sho/J |
| Stock Number: | 005653 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
|---|---|
| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Use restrictions apply, see Policy on Licensing and Use Restrictions.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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