Strain Name:

B6C3-Del(16Cbr1-ORF9)1Rhr/J

Stock Number:

005654

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6C3.129S6-Del(16Cbr1-ORF9)1Rhr/J    (Changed: 19-JAN-06 )
Type Deletion;
Additional information on Mice with Chromosomal Aberrations.
Type Mutant Stock;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
Generation?+N1p (15-JAN-06)
Generation Definitions
 
Donating Investigator Roger H. Reeves,   Johns Hopkins Univ. Schl. Med

Description
These mice are monosomic for the mouse chromosome segment orthologous to the human chromosome 21 Down syndrome critical region (DSCR). The deleted segment contains mouse orthologs of 33 conserved and minimally conserved genes in the human DSCR. The borders of the deletion are defined by the carbonyl reductase 1 (Cbr1) gene and a site adjacent to the myxovirus (influenza virus) resistance 2 (Mx2) locus. Monosomic mice are viable, fertile and are significantly smaller than wildtype (euploid) littermates from birth to adulthood. This mouse may be useful in studies of Down syndrome and further exploring the ploidy of the DSCR segment of mouse Chr 16.

Development
A targeting vector containing a 3' portion of a neomycin resistance gene, a loxP site, and a hygromycin resistance gene at the (Cbr1) locus at the proximal end of the DSCR on MMU16 was electroporated into 129S6/SvEv-derived MC1 embryonic stem (ES) cells. Correctly targeted ES cells were then electroporated with a second targeting vector designed to orient a puromycin resistance gene, a loxP site, and a 5' portion of a neomycin resistance gene adjacent to the (Mx2) locus at the distal end of MMU16. Next, a CAGGS-cre expression vector was electroporated into ES cells harboring both targeted mutations. In ES cells where each construct inserted in trans on homologous chromosomes, recombination between the loxP sites resulted in mice with a duplication of the MMU16 region between the loxP sites on one homologous chromosome and interstitial deletion of the MMU16 region between the loxP sites on the other homologous chromosome. Heterozygous ES cells were injected into blastocysts and then transferred to surrogate mothers. F1 progeny of chimeric mice were bred to (C57BL/6J x C3H/HeJ)Fn to produce mice with segmental monosomy of MMU16 (Stock# 5654 -Del(16Cbr1-ORF9)1Rhr) or segmental trisomy (see Stock# 5383 -Dp(16Cbr1-ORF9)1Rhr). Monosomic mice were crossed with (C57BL/6J x C3H/HeJ)F1 for 4 generationsbefore arriving at The Jackson Laboratory. Upon arrival, this mutant strain has been maintained by breeding with B6C3F1/J.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Related Strains

View Deletion     (6 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
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Dp(16Cbr1-ORF9)1Rhr/0

        involves: 129S6/SvEvTac * C57BL/6
  • nervous system phenotype
  • decreased brain size
    • brain size is 85% of that in control mice   (MGI Ref ID J:121764)
  • small cerebellum
    • cerebellum size if 95% of that in control mice   (MGI Ref ID J:121764)
  • growth/size/body phenotype
  • increased body size   (MGI Ref ID J:93223)
    • increased body weight   (MGI Ref ID J:121764)
  • skeleton phenotype
  • abnormal mandible morphology
    • mandibles are overall enlarged   (MGI Ref ID J:93223)
  • enlarged cranium   (MGI Ref ID J:93223)
  • long femur   (MGI Ref ID J:93223)
  • craniofacial phenotype
  • abnormal mandible morphology
    • mandibles are overall enlarged   (MGI Ref ID J:93223)
  • enlarged cranium   (MGI Ref ID J:93223)
  • limbs/digits/tail phenotype
  • long femur   (MGI Ref ID J:93223)

Dp(16Cbr1-ORF9)1Rhr/0

        B6.129S6-Dp(16Cbr1-ORF9)1Rhr
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype
    • mice perform similar to control mice in a Morris water maze with a visible or hidden platform and have normal long term potentials   (MGI Ref ID J:121764)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Developmental Biology Research
Growth Defects

Neurobiology Research
Down syndrome

Research Tools
Developmental Biology Research

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Del(16Cbr1-ORF9)1Rhr
Allele Name deletion, chromosome 16, R H Reeves 1
Allele Type Targeted (other)
Common Name(s) Del1Rhr; Ms1Rhr;
Mutation Made By Roger Reeves,   Johns Hopkins Univ. Schl. Med
Strain of Origin129S6/SvEvTac
ES Cell Line NameMC1
ES Cell Line Strain129S6/SvEvTac
Gene Symbol and Name Del(16Cbr1-ORF9)1Rhr, deletion, Chr16, R H Reeves 1
Chromosome 16
Gene Common Name(s) Del(16Mx2-Cbr1)1Rhr; Del1Rhr;
General Note A line of mice with segmental monosomy for Chr 16 was derived from descendants of the chimeric founder.
Molecular Note Deletion of a 3.9 Mb segment of Chr 16 was engineered by cre-mediated recombination between single loxP sites inserted into homologous chromosomes. This deleted segment contains mouse orthologs of 33 conserved and minimally conserved genes in the human Down syndrome critical region. [MGI Ref ID J:93223]

Genotyping

Genotyping Information

Genotyping Protocols

Del(16Cbr1-ORF9)1Rhr, Separated PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Olson LE; Richtsmeier JT; Leszl J; Reeves RH. 2004. A chromosome 21 critical region does not cause specific down syndrome phenotypes. Science 306(5696):687-90. [PubMed: 15499018]  [MGI Ref ID J:93223]

Additional References

Del(16Cbr1-ORF9)1Rhr related

Blank M; Fuerst PG; Stevens B; Nouri N; Kirkby L; Warrier D; Barres BA; Feller MB; Huberman AD; Burgess RW; Garner CC. 2011. The Down Syndrome Critical Region Regulates Retinogeniculate Refinement. J Neurosci 31(15):5764-5776. [PubMed: 21490218]  [MGI Ref ID J:170968]

Olson LE; Roper RJ; Sengstaken CL; Peterson EA; Aquino V; Galdzicki Z; Siarey R; Pletnikov M; Moran TH; Reeves RH. 2007. Trisomy for the Down syndrome 'critical region' is necessary but not sufficient for brain phenotypes of trisomic mice. Hum Mol Genet 16(7):774-82. [PubMed: 17339268]  [MGI Ref ID J:121764]

Sussan TE; Yang A; Li F; Ostrowski MC; Reeves RH. 2008. Trisomy represses Apc(Min)-mediated tumours in mouse models of Down's syndrome. Nature 451(7174):73-5. [PubMed: 18172498]  [MGI Ref ID J:131046]

Zhang L; Meng K; Jiang X; Liu C; Pao A; Belichenko PV; Kleschevnikov AM; Josselyn S; Liang P; Ye P; Mobley WC; Yu YE. 2014. Human chromosome 21 orthologous region on mouse chromosome 17 is a major determinant of Down syndrome-related developmental cognitive deficits. Hum Mol Genet 23(3):578-89. [PubMed: 24041763]  [MGI Ref ID J:204610]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, monosomic mice are bred to wildtype B6C3F1/J. Loss of reproductive function has been observed following backcross to C57BL/6J after only 2 generations. C57BL/6 is not recommended for use in breeding this strain.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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