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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation N25p (25-DEC-05) Donating Investigator Alexander Rudensky, University of Washington Description
This transgenic strain carries a rearranged T cell receptor that recognizes a complex consisting of a short peptide derived from the H2-Ea gene (Ealpha52-68) and the H2-Ab gene product (IAb). This mouse lacks naturally occurring Ealpha52-68 peptide as the H2-Ea gene in C57BL/6J mice is incapable of expressing a protein product. Flow cytometric analysis shows 90% and 95% of CD4+ splenocytes expressing Tcra-V2 and Tcrb-V6, respectively. Mice that are hemizygous for these transgenes are viable, fertile and normal in size. This mutant mouse may be useful in studies of positive/negative selection, T cell receptor interactions, or graft-versus-host disease.Development
Transgenic constructs containing the mouse alpha and beta chain of the T cell receptor specific for Ealpha52-68 peptides bound to IAb were cloned from the Tcra-V2 and Tcrb-V6 expressing T cell hybridoma p8.3. The constructs were co-injected into fertilized (C57BL/6J x DBA/2)F2 eggs. Germ line transmission of the transgene was confirmed in founder line 3 and subsequently backcrossed to C57BL/6J for 23 generations.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Tcra
View Strains carrying other alleles of Tcra (35 strains)
Additional Web Information
Congenic Nomenclature
Genetic Quality Control Annual Report
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Tg(Tcra,Tcrb)3Ayr/0
involves: C57BL/6 * DBA/2
- immune system phenotype
- abnormal lymphocyte anergy (MGI Ref ID J:80775)
- cells are hyporesponsive to antigenic peptide challenge
- abnormal positive T cell selection (MGI Ref ID J:125548)
- positive selection of CD4+ T cells is significantly greater in the thymus compared to Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr mice
- decreased T cell proliferation (MGI Ref ID J:80775)
- actively proliferating cells are lower in number than proliferating control cells
- hematopoietic system phenotype
- abnormal positive T cell selection (MGI Ref ID J:125548)
- positive selection of CD4+ T cells is significantly greater in the thymus compared to Tg(Tcra51-11.5,Tcrb51-11.5)AR206Ayr mice
- decreased T cell proliferation (MGI Ref ID J:80775)
- actively proliferating cells are lower in number than proliferating control cells
This mouse can be used to support research in many areas including:
Tcra relatedImmunology and Inflammation Research
Rearranged Antigen-Specific T Cell Receptor Transgenes
Research Tools
Immunology and Inflammation Research (T Cell Receptor Transgenics)
Tcrb relatedHematological Research
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Research Tools
Cancer Research (specific T cell deficiency)
Hematological Research
Immunological Defects
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Immunodeficiency
Inflammation
T Cell Receptor Signaling Defects
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Tcra,Tcrb)3Ayr | ||
|---|---|---|---|
| Allele Name | transgene insertion 3, Alexander Y Rudensky | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | TEa; Tg(Tcra/Tcrb)1Ayr; | ||
| Mutation Made By | Alexander Rudensky, University of Washington | ||
| Strain of Origin | (C57BL/6 x DBA/2)F2 | ||
| Expressed Gene | Tcrb, T-cell receptor beta chain, mouse, laboratory | ||
| Expressed Gene | Tcra, T-cell receptor alpha chain, mouse, laboratory | ||
| Molecular Note | Rearranged Tcra and Tcrb cDNA sequences derived from a Valpha2Vbeta6-expressing T cell hybridoma, p8.3, were used for the transgene constructs. [MGI Ref ID J:84193] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Tcra,Tcrb)3Ayr, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Grubin CE; Kovats S; deRoos P; Rudensky AY. 1997. Deficient positive selection of CD4 T cells in mice displaying altered repertoires of MHC class II-bound self-peptides. Immunity 7(2):197-208. [PubMed: 9285405] [MGI Ref ID J:84193]
Additional References
Tg(Tcra,Tcrb)3Ayr relatedBrabb T; von Dassow P; Ordonez N; Schnabel B; Duke B; Goverman J. 2000. In situ tolerance within the central nervous system as a mechanism for preventing autoimmunity. J Exp Med 192(6):871-80. [PubMed: 10993917] [MGI Ref ID J:107210]
Firpo EJ; Kong RK; Zhou Q; Rudensky AY; Roberts JM; Franza BR. 2002. Antigen-specific dose-dependent system for the study of an inheritable and reversible phenotype in mouse CD4+ T cells. Immunology 107(4):480-8. [PubMed: 12460193] [MGI Ref ID J:80775]
Ledgerwood LG; Lal G; Zhang N; Garin A; Esses SJ; Ginhoux F; Merad M; Peche H; Lira SA; Ding Y; Yang Y; He X; Schuchman EH; Allende ML; Ochando JC; Bromberg JS. 2008. The sphingosine 1-phosphate receptor 1 causes tissue retention by inhibiting the entry of peripheral tissue T lymphocytes into afferent lymphatics. Nat Immunol 9(1):42-53. [PubMed: 18037890] [MGI Ref ID J:130407]
Marie JC; Liggitt D; Rudensky AY. 2006. Cellular mechanisms of fatal early-onset autoimmunity in mice with the T cell-specific targeting of transforming growth factor-beta receptor. Immunity 25(3):441-54. [PubMed: 16973387] [MGI Ref ID J:113553]
Nurieva R; Thomas S; Nguyen T; Martin-Orozco N; Wang Y; Kaja MK; Yu XZ; Dong C. 2006. T-cell tolerance or function is determined by combinatorial costimulatory signals. EMBO J 25(11):2623-33. [PubMed: 16724117] [MGI Ref ID J:109521]
Ravindran R; Rusch L; Itano A; Jenkins MK; McSorley SJ. 2007. CCR6-dependent recruitment of blood phagocytes is necessary for rapid CD4 T cell responses to local bacterial infection. Proc Natl Acad Sci U S A 104(29):12075-12080. [PubMed: 17615242] [MGI Ref ID J:123150]
Wong P; Goldrath AW; Rudensky AY. 2000. Competition for specific intrathymic ligands limits positive selection in a TCR transgenic model of CD4+ T cell development. J Immunol 164(12):6252-9. [PubMed: 10843678] [MGI Ref ID J:125548]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are maintained as hemizygotes.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Additional Supply Details
| Pricing for International shipping destinations |
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*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
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