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Strain Name:

STOCK Tg(Neurog3-cre)C1Able/J

Stock Number:

005667

Availability:

Repository-Cryopreserved


General Terms and Conditions

Genes & Alleles   Gnat2;   Gnat2cpfl3;   Neurog3;   cre;   Tg(Neurog3-cre)C1Able;


Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator Andrew Leiter,   Univ. of Massachusetts Medical School

Important Note
This strain is homozygous for Gnat2cpfl3, cone photoreceptor function loss 3, which affects bright light (photopic) vision.

Strain Description
Mice hemizygous for the transgenic insert are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Expression of the transgene is directed by a neurogenin 3 promoter. Tissues where Cre recombinase expression is detected include the small intestine (base of intestinal crypts) and fetal pancreatic epithelial cells. Cre activity has been shown in islets of the adult pancreas, small intestine enteroendocrine cells, endocrine portions of the stomach, all pancreatic endocrine cells, and in some non-endocrine intestinal cells. When bred with a mouse containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked gene in the tissues that normally express neurogenin 3.

Strain Development
A bacterial artificial chromosome (BAC) encoding the mouse neurogenin 3 sequence was modified by the insertion of a Cre recombinase gene, preceded by nuclear localization sequence, into exon 1 of the neurogenin 3 gene at the initiator methionine. The BAC was injected into FVB/N embryos which were next implanted into pseudopregnant CD1 females. Founder line C1 was obtained and backcrossed to CD1 mice for 7 generations after which mice were intercrossed.

Gene & Allele Details

Allele Symbol Tg(Neurog3-cre)C1Able
Allele Name transgene insertion C1, Andrew B Leiter
Common Name(s) Tg(Neurog3-cre)#Able; ngn3-Cre;
Mutation Made By Andrew Leiter,   Univ. of Massachusetts Medical School
Site of Expressionsmall intestine (base of intestinal crypts) and fetal pancreatic epithelial cells
Expressed Gene cre, cre recombinase, bacteriophage P1
Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence.
Promoter Neurog3, neurogenin 3, rat
General Note This is one of two independent lines demonstrating identical expression patterns.
Molecular Note The Cre recombinase coding sequence, flanked by a nuclear localization signal and an SV40 polyadenylation site, was inserted just after the translation start site of the neurogenin 3 gene present in mouse BAC RP23-121F10, which also includes approximately 81 kb of 5' and 102 kb of 3' flanking DNA. Immunohistochemical analysis shows the same expression pattern for cre as for neurogenin 3 in adult and embryonic mice bearing this transgene. Cre recombinase activity is seen in cell types of pancreas, stomach, small intestine and colon that express or are descended from cells that express neurogenin 3 during development. [MGI Ref ID J:92204]
 
Allele Symbol Gnat2cpfl3
Allele Name cone photoreceptor function loss 3
Common Name(s) Gnat2;
Strain of Originvarious
Gene Symbol and Name Gnat2, guanine nucleotide binding protein, alpha transducing 2
Chromosome 3
Gene Common Name(s) ACHM4; AW490837; GNATC; Gnat-2; Gt-2; Tcalpha; expressed sequence AW490837;
General Note This allele has been detected in the following strains either by genotyping or complementation testing: ALS/LtJ, SENCARA/PtJ, SENCARB/PtJ, SENCARC/PtJ, PN/nBSwUmabJ. (J:122428)

Phenotypic Similarity to Human Syndrome in Orthologous Human Gene: OMIM +139340 ACHROMATOPSIA 4.

Molecular Note A single nucleotide substitution of G to A at position 598 in exon 6. This mutation converts codon 200 from apartic acid to asparagine. [MGI Ref ID J:122428]

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls

Genotyping Protocols

Generic Cre

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are maintained as hemizygotes.

Related Strains

Strains carrying   Gnat2cpfl3 allele
002448   129S1/SvImJ
003072   ALS/LtJ
006180   CD10/JlsJ
005052   PN/nBSwUmabJ
002746   SENCARA/PtJ
002747   SENCARB/PtJ
002748   SENCARC/PtJ
006135   STOCK Sgk3fz-ica/McirJ
003773   STOCK Tg(CAG-ECFP)CK6Nagy/J
005645   STOCK Tg(CAG-mRFP1)1F1Hadj/J
004623   STOCK Tg(Fos-lacZ)34Efu/J
003262   STOCK Tg(Trp53A135V)L3Ber/J
005104   STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
005699   STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
View Strains carrying   Gnat2cpfl3     (14 strains)

Strains carrying   Tg(Neurog3-cre)C1Able allele
006333   B6.FVB(Cg)-Tg(Neurog3-cre)C1Able/J
View Strains carrying   Tg(Neurog3-cre)C1Able     (1 strain)

Strains carrying other alleles of cre
008569   129-Alpltm1(cre)Nagy/J
003328   129-Tg(Prm-cre)58Og/J
004337   129.Cg-Foxg1tm1(cre)Skm/J
005989   129;FVB-Tg(PTH-cre)4167Slib/J
004302   129S1-Hprt1tm1(cre)Mnn/J
003960   129S6-Tg(Prnp-GFP/cre)1Blw/J
005697   B6.129-Otx1tm4(cre)Asim/J
004146   B6.129-Tg(Pcp2-cre)2Mpin/J
006785   B6.129P2(C)-Cd19tm1(cre)Cgn/J
006084   B6.129P2(Cg)-Foxg1tm1(cre)Skm/J
004781   B6.129P2-Lyz2tm1(cre)Ifo/J
005623   B6.129S-Shhtm2(cre/ESR1)Cjt/J
006600   B6.129S1-Mnx1tm4(cre)Tmj/J
005628   B6.129S2-Emx1tm1(cre)Krj/J
003755   B6.129S4-Meox2tm1(cre)Sor/J
006878   B6.129S6-Taglntm2(cre)Yec/J
006054   B6.C-Tg(CMV-cre)1Cgn/J
006230   B6.Cg-Cebpatm1Dgt Tg(Mx1-cre)1Cgn/J
005622   B6.Cg-Shhtm1(EGFP/cre)Cjt/J
006149   B6.Cg-Tg(ACTA1-cre)79Jme/J
003574   B6.Cg-Tg(Alb-cre)21Mgn/J
006881   B6.Cg-Tg(Aqp2-cre)1Dek/J
004682   B6.Cg-Tg(CAG-cre/Esr1)5Amc/J
005359   B6.Cg-Tg(Camk2a-cre)T29-1Stl/J
006137   B6.Cg-Tg(Cdh5-cre)7Mlia/J
006368   B6.Cg-Tg(Cr2-cre)3Cgn/J
006663   B6.Cg-Tg(Eno2-cre)39Jme/J
005069   B6.Cg-Tg(Fabp4-cre)1Rev/J
003573   B6.Cg-Tg(Ins2-cre)25Mgn/J
008068   B6.Cg-Tg(Itgax-cre)1-1Reiz/J
003802   B6.Cg-Tg(Lck-cre)548Jxm/J
003556   B6.Cg-Tg(Mx1-cre)1Cgn/J
007742   B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J
005657   B6.Cg-Tg(Myh6-cre/Esr1)1Jmk/J
003771   B6.Cg-Tg(Nes-cre)1Kln/J
005975   B6.Cg-Tg(Plp1-cre/ESR1)3.16Pop/J
005584   B6.Cg-Tg(Prrx1-cre)1Cjt/J
003967   B6.Cg-Tg(Rbp3-cre)528Jxm/J
008454   B6.Cg-Tg(Sox2-cre)1Amc/J
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
003966   B6.Cg-Tg(Syn1-cre)671Jxm/J
004128   B6.Cg-Tg(Tek-cre)12Flv/J
007606   B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J
008085   B6.Cg-Tg(UBC-cre/ESR1)1Ejb/J
006234   B6.Cg-Tg(tetO-cre)1Jaw/J
006475   B6.FVB(129S4)-Tg(Ckmm-cre)5Khn/J
006451   B6.FVB(129X1)-Tg(Sim1-cre)1Lowl/J
003724   B6.FVB-Tg(EIIa-cre)C5379Lmgd/J
004586   B6.SJL-Tg(Vil-cre)997Gum/J
005650   B6129-Tg(Myh6-cre/Esr1)1Jmk/J
003552   B6129-Tg(Wap-cre)11738Mam/J
004847   B6;129-Gt(ROSA)26Sortm1(cre/Esr1)Nat/J
005549   B6;129-Pax3tm1(cre)Joe/J
006668   B6;129P2-Omptm4(cre)Mom/MomJ
007001   B6;129S-Tg(UBC-cre/ESR1)1Ejb/J
006410   B6;129S6-Chattm1(cre)Lowl/J
003466   B6;D2-Tg(Sycp1-cre)4Min/J
003734   B6;FVB-Tg(GZMB-cre)1Jcb/J
006302   B6;SJL-Slc6a3tm1.1(cre)Bkmn/J
004426   B6;SJL-Tg(Cga-cre)3Sac/J
003554   B6;SJL-Tg(Col2a1-cre)1Bhr/J
005249   B6;SJL-Tg(Krt1-15-cre/PGR)22Cot/J
007610   B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J
007252   B6Ei.129S4-Tg(Prm-cre)58Og/EiJ
003465   BALB/c-Tg(CMV-cre)1Cgn/J
004126   C.129P2-Cd19tm1(cre)Cgn/J
005673   C.Cg-Tg(Mx1-cre)1Cgn/J
006244   C.Cg-Tg(tetO-cre)1Jaw/J
006474   C57BL/6-Tg(Grik4-cre)G32-4Stl/J
006888   C57BL/6-Tg(Zp3-cre)1Gwh/J
003394   C57BL/6-Tg(Zp3-cre)3Mrt/J
003651   C57BL/6-Tg(Zp3-cre)93Knw/J
007567   C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
006405   FVB-Tg(Ckmm-cre)5Khn/J
006774   FVB-Tg(Col2a1-cre/ESR1)KA3Smac/J
006954   FVB-Tg(Ddx4-cre)1Dcas/J
004600   FVB-Tg(GFAP-cre)25Mes/J
006364   FVB-Tg(Nr5a1-cre)2Lowl/J
006139   FVB.Cg-Tg(ACTA1-cre)79Jme/J
006297   FVB.Cg-Tg(Eno2-cre)39Jme/J
008244   FVB.Cg-Tg(tetO-cre)1Jaw/J
003376   FVB/N-Tg(ACTB-cre)2Mrt/J
003314   FVB/N-Tg(EIIa-cre)C5379Lmgd/J
006143   FVB/N-Tg(Thy1-cre)1Vln/J
003377   FVB/N-Tg(Zp3-cre)3Mrt/J
005732   NOD.Cg-Tg(Lck-cre)548Jxm/AchJ
004986   NOD/ShiLt-Tg(Ins2-cre)3Lt/Lt
003855   NOD/ShiLt-Tg(Ins2-cre)5Lt/LtJ
004987   NOD/ShiLt-Tg(Ins2-cre)6Lt/Lt
004192   STOCK Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy Tg(Mx1-cre)1Cgn/J
006677   STOCK Olfr151tm28Mom/MomJ
005936   STOCK Tg(ACTA1-cre)79Jme/J
007684   STOCK Tg(Atoh1-cre/ESR1)14Fsh/J
004453   STOCK Tg(CAG-cre/Esr1)5Amc/J
005105   STOCK Tg(Chx10-EGFP/cre-ALPP)2Clc/J
005938   STOCK Tg(Eno2-cre)39Jme/J
004692   STOCK Tg(Hoxb7-cre)13Amc/J
008122   STOCK Tg(Ins2-cre/Esr1)1Dam/J
004782   STOCK Tg(KRT14-cre)1Amc/J
005107   STOCK Tg(KRT14-cre/Esr1)20Efu/J
003551   STOCK Tg(MMTV-cre)1Mam/J
003553   STOCK Tg(MMTV-cre)4Mam/J
002527   STOCK Tg(Mx1-cre)1Cgn/J
002858   STOCK Tg(Nes-cre)1Wme/J
002859   STOCK Tg(Nes-cre)2Wme/J
008119   STOCK Tg(Neurog3-cre/Esr1)1Dam/J
006207   STOCK Tg(Pcp2-cre)1Amc/J
005965   STOCK Tg(Pomc1-cre)16Lowl/J
006395   STOCK Tg(Sim1-cre)1Lowl/J
004783   STOCK Tg(Sox2-cre)1Amc/J
004746   STOCK Tg(Tagln-cre)1Her/J
003829   STOCK Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J
002471   STOCK Tg(hCMV-cre)140Sau/J
006224   STOCK Tg(tetO-cre)1Jaw/J
View Strains carrying other alleles of cre     (114 strains)

Additional Web Information

Cre-lox or FLP-FRT Systems

Research Applications

This mouse can be used to support research in many areas including:

Endocrine Deficiency Research
Pancreas Defects

Research Tools
Cre-lox System (Cre Recombinase Expression)
Endocrine Deficiency Research
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genetics Research (Tissue/Cell Markers: Cre-lox System)

cre related

Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)

References

Selected Reference(s)

Schonhoff SE; Giel-Moloney M; Leiter AB. 2004. Neurogenin 3-expressing progenitor cells in the gastrointestinal tract differentiate into both endocrine and non-endocrine cell types. Dev Biol 270(2):443-54. [PubMed: 15183725]  [MGI Ref ID J:92204]

Additional References

Price and Supply Information

Strain Name: STOCK Tg(Neurog3-cre)C1Able/J
Stock Number: 005667

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryopreserved Embryos
This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page.
Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

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Control InformationView Control Information in Strain Details.

General Terms and Conditions

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Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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