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| When these gene targeted mice are bred to transgenic strains expressing Cre recombinase, functional rtTA and EGFP activity is observed in the double mutant offspring in the tissues that express cre. These double mutant mice may be bred to transgenic strains carrying genes of interest under the regulation of tetracycline responsive elements (TRE; tetO) to generate triple mutant mice in which the tissue specificity of the cre-transgenic line and doxycycline inducibility of the rtTA/TRE-controlled transgenes can be combined to regulate expression of the target gene. | |||||||||||||||||||
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- Disease & phenotype
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Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 29-FEB-08 Species laboratory mouse Generation F?+F1N10F9 (27-NOV-11)
Generation DefinitionsDonating Investigator IMR Colony, The Jackson Laboratory Description
Homozygous mutant mice are viable, fertile, normal in size and do not display any behavioral abnormalities. When these gene targeted mice are bred to transgenic strains expressing Cre recombinase, functional rtTA and EGFP activity is observed in the double mutant offspring in the tissues that express cre. These double mutant mice may be bred to transgenic strains carrying genes of interest under the regulation of tetracycline responsive elements (TRE; tetO) to generate triple mutant mice in which the tissue specificity of the cre-transgenic line and doxycycline inducibility of the rtTA/TRE-controlled transgenes can be combined to regulate expression of the target gene.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A targeting vector containing a loxP-flanked phosphoglycerate kinase-neomycin resistance gene -polyadenylation stop sequence (PGK-neo-pA) fragment and a downstream reverse tetracycline-controlled transactivator gene-internal ribosomal entry site-enhanced green fluorescent protein gene-polyadenylation stop sequence (rtTA-IRES-EGFP-pA) fusion protein was inserted between exon 1 and 2 of the endogenous Gt(ROSA)26Sor locus. The construct was electroporated into (129X1/SvJ x 129S1/Sv)-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into ICR blastocysts and the resulting chimeric males were backcrossed for germ-line transmission to ICR females. Heterozygous offspring were sent to The Jackson Laboratory (as Stock No. 005572). Upon arrival, some mice were backcrossed to C57BL/6J for at least 5 generations to generate this congenic strain (Stock No. 005670).
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (357 strains)
005572 STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cre-lox System
loxP-flanked Sequences
loxP-flanked Sequences: Test/Reporter
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Mutagenesis and Transgenesis: Tetop Tet System
Tissue/Cell Markers
Tissue/Cell Markers: Cre-lox System
Tet Expression Systems
tTA/rtTA Expressing Strains
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Andras Nagy | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Gt(ROSA)26Sortm1(rtTA)Nagy; Gt(ROSA)26Sortm1Nagy; R26rtTA; ROSA26-rtTA; ROSA26-rtTA-IRES-EGFP; Rosa26fs-rtTA; rtTAflox; | ||
| Mutation Made By | Gusztav Belteki and Jody Haigh, Mount Sinai Hospital | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | When these mutant mice are crossed to a strain expressing Cre recombinase, rtTA and EGFP are expressed in the cre-expressing tissues. These mice can be used to generate mutant mice in which the tissue specificity of cre-expression and doxycycline inducibility of the rtTA can be combined. | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Molecular Note | A targeting vector containing a floxed Pgk-neo-pA cassette and a rtTA-IRES-EGFP-pA cassette was inserted into intron 1 of the locus. Upon cre expression, the floxed neo cassette is removed and the ROSA26 promoter drives expression of rtTA and EGFP. Presence of doxycycline results in the formation of an active transcriptional activator and the activation of the responder transgene. [MGI Ref ID J:80963] [MGI Ref ID J:99607] | ||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Neo Melt Curve Analysis,Robotic MCA
Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy, Separated PCR
ROSA26 Generic GFP, Melt Curve Analysis
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Belteki G; Haigh J; Kabacs N; Haigh K; Sison K; Costantini F; Whitsett J; Quaggin SE; Nagy A. 2005. Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction. Nucleic Acids Res 33(5):e51. [PubMed: 15784609] [MGI Ref ID J:99607]
Additional References
Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy relatedBonilla-Claudio M; Wang J; Bai Y; Klysik E; Selever J; Martin JF. 2012. Bmp signaling regulates a dose-dependent transcriptional program to control facial skeletal development. Development 139(4):709-19. [PubMed: 22219353] [MGI Ref ID J:181229]
Collins MA; Bednar F; Zhang Y; Brisset JC; Galban S; Galban CJ; Rakshit S; Flannagan KS; Adsay NV; Pasca di Magliano M. 2012. Oncogenic Kras is required for both the initiation and maintenance of pancreatic cancer in mice. J Clin Invest 122(2):639-53. [PubMed: 22232209] [MGI Ref ID J:184378]
Collins MA; Brisset JC; Zhang Y; Bednar F; Pierre J; Heist KA; Galban CJ; Galban S; di Magliano MP. 2012. Metastatic pancreatic cancer is dependent on oncogenic Kras in mice. PLoS One 7(12):e49707. [PubMed: 23226501] [MGI Ref ID J:195686]
Deng Y; Wang ZV; Tao C; Gao N; Holland WL; Ferdous A; Repa JJ; Liang G; Ye J; Lehrman MA; Hill JA; Horton JD; Scherer PE. 2013. The Xbp1s/GalE axis links ER stress to postprandial hepatic metabolism. J Clin Invest 123(1):455-68. [PubMed: 23257357] [MGI Ref ID J:194290]
Drogat B; Kalucka J; Gutierrez L; Hammad H; Goossens S; Farhang Ghahremani M; Bartunkova S; Haigh K; Deswarte K; Nyabi O; Naessens M; Ferrara N; Klingmuller U; Lambrecht BN; Nagy A; Philipsen S; Haigh JJ. 2010. Vegf regulates embryonic erythroid development through Gata1 modulation. Blood 116(12):2141-51. [PubMed: 20554972] [MGI Ref ID J:164506]
El Andaloussi A; Graves S; Meng F; Mandal M; Mashayekhi M; Aifantis I. 2006. Hedgehog signaling controls thymocyte progenitor homeostasis and differentiation in the thymus. Nat Immunol 7(4):418-26. [PubMed: 16518394] [MGI Ref ID J:112555]
Eshkar-Oren I; Viukov SV; Salameh S; Krief S; Oh CD; Akiyama H; Gerber HP; Ferrara N; Zelzer E. 2009. The forming limb skeleton serves as a signaling center for limb vasculature patterning via regulation of Vegf. Development 136(8):1263-72. [PubMed: 19261698] [MGI Ref ID J:147285]
Grachtchouk M; Pero J; Yang SH; Ermilov AN; Michael LE; Wang A; Wilbert D; Patel RM; Ferris J; Diener J; Allen M; Lim S; Syu LJ; Verhaegen M; Dlugosz AA. 2011. Basal cell carcinomas in mice arise from hair follicle stem cells and multiple epithelial progenitor populations. J Clin Invest 121(5):1768-81. [PubMed: 21519145] [MGI Ref ID J:173930]
Gupte VV; Ramasamy SK; Reddy R; Lee J; Weinreb PH; Violette SM; Guenther A; Warburton D; Driscoll B; Minoo P; Bellusci S. 2009. Overexpression of fibroblast growth factor-10 during both inflammatory and fibrotic phases attenuates bleomycin-induced pulmonary fibrosis in mice. Am J Respir Crit Care Med 180(5):424-36. [PubMed: 19498056] [MGI Ref ID J:167964]
He N; Xiao Z; Yin T; Stubbs J; Li L; Quarles LD. 2010. Inducible expression of Runx2 results in multiorgan abnormalities in mice. J Cell Biochem :. [PubMed: 21136430] [MGI Ref ID J:171769]
He N; Xiao Z; Yin T; Stubbs J; Li L; Quarles LD. 2011. Inducible expression of Runx2 results in multiorgan abnormalities in mice. J Cell Biochem 112(2):653-65. [PubMed: 21268087] [MGI Ref ID J:168061]
Jeansson M; Gawlik A; Anderson G; Li C; Kerjaschki D; Henkelman M; Quaggin SE. 2011. Angiopoietin-1 is essential in mouse vasculature during development and in response to injury. J Clin Invest 121(6):2278-89. [PubMed: 21606590] [MGI Ref ID J:174022]
Kelly MC; Chang Q; Pan A; Lin X; Chen P. 2012. Atoh1 directs the formation of sensory mosaics and induces cell proliferation in the postnatal Mammalian cochlea in vivo. J Neurosci 32(19):6699-710. [PubMed: 22573692] [MGI Ref ID J:184847]
Kim ST; Adair-Kirk TL; Senior RM; Miner JH. 2012. Functional consequences of cell type-restricted expression of laminin alpha5 in mouse placental labyrinth and kidney glomerular capillaries. PLoS One 7(7):e41348. [PubMed: 22911783] [MGI Ref ID J:189883]
Kocabas F; Zheng J; Thet S; Copeland NG; Jenkins NA; DeBerardinis RJ; Zhang C; Sadek HA. 2012. Meis1 regulates the metabolic phenotype and oxidant defense of hematopoietic stem cells. Blood 120(25):4963-72. [PubMed: 22995899] [MGI Ref ID J:192126]
Le TT; McGovern VL; Alwine IE; Wang X; Massoni-Laporte A; Rich MM; Burghes AH. 2011. Temporal requirement for high SMN expression in SMA mice. Hum Mol Genet 20(18):3578-91. [PubMed: 21672919] [MGI Ref ID J:174960]
Lin HY; Yang LT. 2013. Differential response of epithelial stem cell populations in hair follicles to TGF-beta signaling. Dev Biol 373(2):394-406. [PubMed: 23103542] [MGI Ref ID J:192167]
Maes C; Goossens S; Bartunkova S; Drogat B; Coenegrachts L; Stockmans I; Moermans K; Nyabi O; Haigh K; Naessens M; Haenebalcke L; Tuckermann JP; Tjwa M; Carmeliet P; Mandic V; David JP; Behrens A; Nagy A; Carmeliet G; Haigh JJ. 2010. Increased skeletal VEGF enhances beta-catenin activity and results in excessively ossified bones. EMBO J 29(2):424-41. [PubMed: 20010698] [MGI Ref ID J:156474]
Moriyama M; Fukuhara T; Britschgi M; He Y; Narasimhan R; Villeda S; Molina H; Huber BT; Holers M; Wyss-Coray T. 2011. Complement Receptor 2 Is Expressed in Neural Progenitor Cells and Regulates Adult Hippocampal Neurogenesis. J Neurosci 31(11):3981-3989. [PubMed: 21411641] [MGI Ref ID J:170456]
Noah TK; Kazanjian A; Whitsett J; Shroyer NF. 2010. SAM pointed domain ETS factor (SPDEF) regulates terminal differentiation and maturation of intestinal goblet cells. Exp Cell Res 316(3):452-65. [PubMed: 19786015] [MGI Ref ID J:156638]
Pan W; Jin Y; Stanger B; Kiernan AE. 2010. Notch signaling is required for the generation of hair cells and supporting cells in the mammalian inner ear. Proc Natl Acad Sci U S A 107(36):15798-803. [PubMed: 20733081] [MGI Ref ID J:164383]
Parsa S; Kuremoto K; Seidel K; Tabatabai R; Mackenzie B; Yamaza T; Akiyama K; Branch J; Koh CJ; Al Alam D; Klein OD; Bellusci S. 2010. Signaling by FGFR2b controls the regenerative capacity of adult mouse incisors. Development 137(22):3743-52. [PubMed: 20978072] [MGI Ref ID J:167068]
Parsa S; Ramasamy SK; De Langhe S; Gupte VV; Haigh JJ; Medina D; Bellusci S. 2008. Terminal end bud maintenance in mammary gland is dependent upon FGFR2b signaling. Dev Biol 317(1):121-31. [PubMed: 18381212] [MGI Ref ID J:136174]
Pi M; Chen L; Huang M; Luo Q; Quarles LD. 2008. Parathyroid-specific interaction of the calcium-sensing receptor and G alpha q. Kidney Int 74(12):1548-56. [PubMed: 18813283] [MGI Ref ID J:162715]
Sala FG; Del Moral PM; Tiozzo C; Alam DA; Warburton D; Grikscheit T; Veltmaat JM; Bellusci S. 2011. FGF10 controls the patterning of the tracheal cartilage rings via Shh. Development 138(2):273-82. [PubMed: 21148187] [MGI Ref ID J:167739]
Schlegelmilch K; Mohseni M; Kirak O; Pruszak J; Rodriguez JR; Zhou D; Kreger BT; Vasioukhin V; Avruch J; Brummelkamp TR; Camargo FD. 2011. Yap1 acts downstream of alpha-catenin to control epidermal proliferation. Cell 144(5):782-95. [PubMed: 21376238] [MGI Ref ID J:171057]
Sharir A; Stern T; Rot C; Shahar R; Zelzer E. 2011. Muscle force regulates bone shaping for optimal load-bearing capacity during embryogenesis. Development 138(15):3247-59. [PubMed: 21750035] [MGI Ref ID J:180904]
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Ying H; Kimmelman AC; Lyssiotis CA; Hua S; Chu GC; Fletcher-Sananikone E; Locasale JW; Son J; Zhang H; Coloff JL; Yan H; Wang W; Chen S; Viale A; Zheng H; Paik JH; Lim C; Guimaraes AR; Martin ES; Chang J; Hezel AF; Perry SR; Hu J; Gan B; Xiao Y; Asara JM; Weissleder R; Wang YA; Chin L; Cantley LC; DePinho RA. 2012. Oncogenic Kras maintains pancreatic tumors through regulation of anabolic glucose metabolism. Cell 149(3):656-70. [PubMed: 22541435] [MGI Ref ID J:186194]
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Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry Mutant mice were bred to C57BL/6J mice to generate this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred. Mating System Homozygote x Homozygote (Female x Male) 29-FEB-08 Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $177.00 Female or Male Homozygous for Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy
Price per Pair (US dollars $) Pair Genotype $354.00 Homozygous for Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy x Homozygous for Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $230.10 Female or Male Homozygous for Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy
Price per Pair (US dollars $) Pair Genotype $460.20 Homozygous for Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy x Homozygous for Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
|
|
|
Standard Supply
Repository-Live. Repository-Live represents an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. Repository-live orders are treated as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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