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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 29-FEB-08 Species laboratory mouse Generation N13 (19-MAY-09) Donating Investigator IMR Colony, The Jackson Laboratory Description
Homozygous mutant mice are viable, fertile, normal in size and do not display any behavioral abnormalities. When these gene targeted mice are bred to transgenic strains expressing Cre recombinase, functional rtTA and EGFP activity is observed in the double mutant offspring in the tissues that express cre. These double mutant mice may be bred to transgenic strains carrying genes of interest under the regulation of tetracycline responsive elements (TRE; tetO) to generate triple mutant mice in which the tissue specificity of the cre-transgenic line and doxycycline inducibility of the rtTA/TRE-controlled transgenes can be combined to regulate expression of the target gene.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Development
A targeting vector containing a loxP-flanked phosphoglycerate kinase-neomycin resistance gene -polyadenylation stop sequence (PGK-neo-pA) fragment and a downstream reverse tetracycline-controlled transactivator gene-internal ribosomal entry site-enhanced green fluorescent protein gene-polyadenylation stop sequence (rtTA-IRES-EGFP-pA) fusion protein was inserted between exon 1 and 2 of the endogenous Gt(ROSA)26Sor locus. The construct was electroporated into (129X1/SvJ x 129S1/Sv)-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into ICR blastocysts and the resulting chimeric males were backcrossed for germ-line transmission to ICR females. Heterozygous offspring were sent to The Jackson Laboratory (as Stock No. 005572). Upon arrival, some mice were backcrossed to C57BL/6J for at least 5 generations to generate this congenic strain (Stock No. 005670).
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
005572 STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
006965 B6.Cg-Gt(ROSA)26Sortm1(rtTA*M2)Jae/J 007176 B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes/J 006235 B6.Cg-Tg(SFTPC-rtTA)5Jaw/J 006232 B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 006911 B6;129-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1tm2(tetO-Pou5f1)Jae/J 007678 B6;SJL-Tg(KRT14-rtTA)208Jek/J 010576 B6;SJL-Tg(MMTV-rtTA)4-1Jek/J 006245 C.Cg-Tg(SFTPC-rtTA)5Jaw/J 006242 C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 008099 FVB-Tg(KRT14-rtTA)F42Efu/J 004127 FVB-Tg(Nes-rtTA)306Rvs/J 006225 FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J 006222 FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 008202 FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J 006875 FVB/N-Tg(Tagln-rtTA)E1Jwst/J 004602 NOD.Cg-Tg(Ins2-rtTA)2Doi/DoiJ 005734 NOD/Lt-Tg(Ins2-rtTA)1Ach/AchJ 008755 STOCK Tg(Ins2-rtTA)2Efr Tg(teto-DTA)1Gfi/J 008250 STOCK Tg(Ins2-rtTA)2Efr/J 005493 STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J 003273 STOCK Tg(rtTAhCMV)4Bjd/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Introduction to Cre-lox technology
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cre-lox System
loxP-flanked Sequences: Test/Reporter
Fluorescent Proteins
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
Mutagenesis and Transgenesis: Tetop Tet System
Tissue/Cell Markers: Cre-lox System
Tet Expression Systems
tTA/rtTA Expressing Strains
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Andras Nagy | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Gt(ROSA)26Sortm1(rtTA)Nagy; Gt(ROSA)26Sortm1Nagy; ROSA26-rtTA-IRES-EGFP; | ||
| Mutation Made By | Gusztav Belteki and Jody Haigh, Mount Sinai Hospital | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | When these mutant mice are crossed to a strain expressing Cre recombinase, rtTA and EGFP are expressed in the cre-expressing tissues. These mice can be used to generate mutant mice in which the tissue specificity of cre-expression and doxycycline inducibility of the rtTA can be combined. | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Gene Symbol and Name | Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | AV258896; Gtrgeo26; Gtrosa26; R26; ROSA26; beta geo; expressed sequence AV258896; gene trap ROSA 26; gene trap ROSA b-geo 26; | ||
| Molecular Note | A targeting vector containing a floxed Pgk-neo-pA cassette and a rtTA-IRES-EGFP-pA cassette was inserted into intron 1 of the locus. Upon cre expression, the floxed neo cassette is removed and the ROSA26 promoter drives expression of rtTA and EGFP. Presence of doxycycline results in the formation of an active transcriptional activator and the activation of the responder transgene. [MGI Ref ID J:80963] [MGI Ref ID J:99607] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy, Melt Curve Analysis
Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy, Separated PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Belteki G; Haigh J; Kabacs N; Haigh K; Sison K; Costantini F; Whitsett J; Quaggin SE; Nagy A. 2005. Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction. Nucleic Acids Res 33(5):e51. [PubMed: 15784609] [MGI Ref ID J:99607]
Additional References
Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy relatedEl Andaloussi A; Graves S; Meng F; Mandal M; Mashayekhi M; Aifantis I. 2006. Hedgehog signaling controls thymocyte progenitor homeostasis and differentiation in the thymus. Nat Immunol 7(4):418-26. [PubMed: 16518394] [MGI Ref ID J:112555]
Eshkar-Oren I; Viukov SV; Salameh S; Krief S; Oh CD; Akiyama H; Gerber HP; Ferrara N; Zelzer E. 2009. The forming limb skeleton serves as a signaling center for limb vasculature patterning via regulation of Vegf. Development 136(8):1263-72. [PubMed: 19261698] [MGI Ref ID J:147285]
Parsa S; Ramasamy SK; De Langhe S; Gupte VV; Haigh JJ; Medina D; Bellusci S. 2008. Terminal end bud maintenance in mammary gland is dependent upon FGFR2b signaling. Dev Biol 317(1):121-31. [PubMed: 18381212] [MGI Ref ID J:136174]
Srinivas S; Watanabe T; Lin CS; William CM; Tanabe Y; Jessell TM; Costantini F. 2001. Cre reporter strains produced by targeted insertion of EYFP and ECFP into the ROSA26 locus. BMC Dev Biol 1(1):4. [PubMed: 11299042] [MGI Ref ID J:80963]
Wehn AK; Gallo PH; Chapman DL. 2009. Generation of transgenic mice expressing Cre recombinase under the control of the Dll1 mesoderm enhancer element. Genesis 47(5):309-313. [PubMed: 19298012] [MGI Ref ID J:148512]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry Mutant mice were bred to C57BL/6J mice to generate this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred. Mating System Homozygote x Homozygote (Female x Male) 29-FEB-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $160.40 Female or Male Homozygous for Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy
Pairs /Price (US dollars $) Pair Genotype $320.80 Homozygous for Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy x Homozygous for Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $208.60 Female or Male Homozygous for Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy
Pairs /Price (US dollars $) Pair Genotype $417.10 Homozygous for Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy x Homozygous for Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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