Strain Name: |
C.Cg-Tg(Mx1-cre)1Cgn/J |
|---|---|
Stock Number: |
005673 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Strain Common Names | Mx1-Cre(BALB/c); |
| Genes & Alleles | Mx1; cre; Tg(Mx1-cre)1Cgn; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Roberta Pelanda, National Jewish Medical and Research Ctr Strain Description
The Cre recombinase is under the control of the Mx1 promoter. This promoter is silent in healthy mice, but can be induced to high levels of transcription by administration of interferon alpha, interferon beta, or synthetic double-stranded RNA. When combined with a mutant carrying a gene that has been flanked by loxP recognition sites, the expression of Cre recombinase causes the flanked gene to be removed. This provides researchers with the capability to induce the "knockout" at any time during development. There is ~1% background recombination seen in mice not treated with interferon. The percent deletion of the targeted gene varies depending on tissue type, presumably due to the amount of interferon-responsive cells present or to the availability of interferon in each organ.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A transgenic construct containing the mouse Mx1 gene promoter, nuclear localization sequence-modified Cre recombinase and a 2.1 kb fragment from the human growth hormone gene (to provide the splicing and polyadenylation signals) was injected into (C57BL/6J X CBA/J)F2 fertilized eggs. The resulting transgenic mice were crossed to (C57BL/6J x 129Sv)F2 mice for an unknown number of generations. The mice were then backcrossed onto the BALB/cJ background for 10 generations.
Gene & Allele Details
| Allele Symbol | Tg(Mx1-cre)1Cgn | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, University of Cologne | ||
| Common Name(s) | Mx-Cre; Mx-Cre 31; Mx1-Cre; Mx1cre; MxCre; | ||
| Mutation Made By | Ralf Kuhn, University of Cologne | ||
| Strain of Origin | (C57BL/6 x CBA)F2 | ||
| Site of Expression | widespread pattern of expression; promoter induced to high levels of transcription by administration of interferon alpha, interferon beta, or synthetic double-stranded RNA; provides capability to induce the "knockout" at any time during development | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Mx1, myxovirus (influenza virus) resistance 1, mouse, laboratory | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of an inducible Mx1 promoter, which is silent in healthy mice, and active in the liver and in lymphocytes after induction with IFN or pI-pC. The construct also contains a 2.1 kb fragment from thehuman growth hormone gene. [J:105040] [J:67927] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000651 BALB/cJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Generic Cre
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are maintained as hemizygotes. |
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Related Strains
Strains carrying Tg(Mx1-cre)1Cgn allele
006230 B6.Cg-Cebpatm1Dgt Tg(Mx1-cre)1Cgn/J 003556 B6.Cg-Tg(Mx1-cre)1Cgn/J 004192 STOCK Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy Tg(Mx1-cre)1Cgn/J 002527 STOCK Tg(Mx1-cre)1Cgn/J View Strains carrying Tg(Mx1-cre)1Cgn (4 strains)
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Research Applications
This mouse can be used to support research in many areas including:
cre relatedResearch Tools
Cre-lox-System (Cre-Recombinase Expression: Inducible)
Genetics Research (Tissue/Cell Markers: Cre-lox system)
Research Tools
Cre-lox-System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox system)
References
Selected Reference(s)
Additional ReferencesKuhn R; Schwenk F; Aguet M; Rajewsky K. 1995. Inducible gene targeting in mice. Science 269(5229):1427-9. [PubMed: 7660125] [J:67927]
Price and Supply Information
| Strain Name: | C.Cg-Tg(Mx1-cre)1Cgn/J |
| Stock Number: | 005673 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
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| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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