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Former Name	C.129S4(B6)-C5ar1tm1Cge/J    (Changed: 02-FEB-07 )
	C.129S4(B6)-C5r1tm1Cge/J    (Changed: 21-FEB-06 )
	C.Cg-C5r1tm1Cge/J    (Changed: 01-SEP-05 )
Genes & Alleles	C5ar1;   C5ar1tm1Cge;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Donating Investigator Craig Gerard,   Childrens' Hospital Boston, Harvard MS Generation ?+N1F4 (31-DEC-07)

Strain Description
Mice homozygous for the targeted mutation are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities when maintained under barrier conditions. No gene product is detected in bone marrow. The immune response of homozygous mice following inoculation or challenge has been characterized on a C57BL/6 background in a number of studies. Following intratracheal inoculation, mutant mice have impaired bacterial clearance in the lungs, associated with extensive secondary infection, despite increased neutrophil accumulation. Conversely, mutants are protected against immune-complex associated injury in the lung, peritoneum, skin, and kidney; but not against experimental autoimmune encephalomyelitis. Induced acute pancreatitis and pancreatitis-associated lung injury is more severe compared to wild type. On a BALB/c background, these mice are more resistant to dermal infection and lymph node cells have greatly increased antigen recall, as measured by interferon gamma secretion, compared to wild type. Homozygotes fail to develop airway hyper-reactivity in hapten asthma models (background not-identified). This mutant can be used to study many lung-associated diseases, immune complex associated injury, and complement innate immunity.

Strain Development
A targeting vector containing a mouse phosphoglycerate kinase promoter driven neomycin resistance gene was used to replace the entire coding region of the endogenous gene. The construct was electroporated into the 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric males were backcrossed to C57BL/6 females. Heterozygotes were next mated to C57BL/6 mice for an unknown number of generations prior to arrival at The Jackson Laboratory. Once arrived, mice were crossed at least once to BALB/cJ. The resulting heterozygous mice were then intercrossed to generate homozygous mice.

Mammalian Phenotype Terms assigned by genotype The following phenotype information may relate to a genetic background differing from this JAX® Mice strain. C5ar1tm1Cge/C5ar1tm1Cge         involves: 129S4/SvJae immune system phenotype abnormal humoral immune response (MGI Ref ID J:35529) after injection of chicken egg albumin in the tail and administration of antibodies to egg albumin intratracheally, the null mice exhibit almost complete protection from inflammatory response upon examination of lung tissue increased susceptibility to bacterial infection (MGI Ref ID J:69177) increased sensitivity to infection by Pseudomonas aeruginosa, death by second day after innoculation

Gene & Allele Details

Allele Symbol		C5ar1tm1Cge
Allele Name		targeted mutation 1, Craig Gerard
Common Name(s)		C5aR-; C5aRKO;
Mutation Made By		Craig Gerard,   Childrens' Hospital Boston, Harvard MS
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		C5ar1, complement component 5a receptor 1
Chromosome		7
Gene Common Name(s)		C5A; C5AR; C5R1; C5r1; CD88; D7Msu1; DNA segment, Chr 7, Michigan State University 1; complement component 5, receptor 1;
Molecular Note		A PGK-neomycin resistance cassette replaced the entire coding region of the gene. Northern analysis of bone marrow cells did not detect expression in homozygous mutant mice. [MGI Ref ID J:69177]

Control Information

	Control
	None Available
Considerations for Choosing Controls

Genotyping Protocols

C5ar1^tm1Cge

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice are maintained as heterozygotes or homozygotes. For homozygous colonies, SPF conditions are recommended as mutant mice have impaired clearance of bacterial infection.
Diet Information	LabDiet® 5K52/5K67

Related Strains

Strains carrying   C5ar1^tm1Cge allele

006845

C.129S4(B6)-C5ar1tm1Cge/J

View Strains carrying   C5ar1^tm1Cge     (1 strain)

Additional Web Information

Congenic Nomenclature
Genetic Quality Control Annual Report

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

C5ar1^tm1Cge related

Immunology and Inflammation Research
Autoimmunity (specific complement deficiency)

Research Tools
Immunology and Inflammation Research (genes regulating susceptibility to infectious disease and endotoxin)
Immunology and Inflammation Research (specific complement deficiency)

References

Selected Reference(s)

Hopken UE; Lu B; Gerard NP; Gerard C. 1996. The C5a chemoattractant receptor mediates mucosal defence to infection. Nature 383(6595):86-9. [PubMed: 8779720]  [MGI Ref ID J:69177]

Additional References

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Price and Supply Information

Strain Name:	STOCK C5ar1tm1Cge/J
Stock Number:	005676

Price Details

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Supply Details

Standard Supply	Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes	Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

General Terms and Conditions

View JAX^® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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