Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation N6+F?+N1p (25-DEC-05)   Donating Investigator Thomas Quertermous,   Stanford University School of Medicine

Description
Homozygous mice are viable and do not display any behavioral or histological defects. Although homozygotes are fertile, the donating investigator reports decreased fecundity. Northern blot analysis showed no endogenous gene expression in lung, liver, kidney, spleen, or aorta. Homozygotes have increased fasting plasma levels of total cholesterol, high-density lipoprotein (HDL) cholesterol, and phospholipids compared to wild type. Mutant mouse plasma apoA-I is increased while heparin-releasable phospholipase activity is impaired. Homozygous null mice have decreased arterial endothelium monocyte adhesion. This mutant can be used in studies of lipoprotein metabolism, atherosclerotic vascular disease, and other cholesterol-related studies.

Development
A targeting vector containing a neomycin resistance gene was used to replace exon 1 of the endogenous gene. The construct was electroporated into 129S6/SvEv-derived TL-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. A resulting chimeric male was backcrossed to a C57BL/6J female. Heterozygous pups were bred to C57BL/6J for at least six generations before being interbred.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Additional Web Information

Congenic Nomenclature
Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Lipg^tm1Tq/Lipg⁺

        B6.129S6-Lipg^tm1Tq

• homeostasis/metabolism phenotype
• increased circulating HDL cholesterol level (MGI Ref ID J:81821)
  • 25% increase in male but not female mice compared to in wild-type mice
• increased circulating phospholipid level (MGI Ref ID J:81821)
  • over 30% increase compared to in wild-type mice
• increased circulating triglyceride level (MGI Ref ID J:81821)
  • in female mice but not male mice

Lipg^tm1Tq/Lipg^tm1Tq

        B6.129S6-Lipg^tm1Tq

• homeostasis/metabolism phenotype
• increased circulating cholesterol level (MGI Ref ID J:81821)
  • both male and female mice exhibit an increase in serum cholesterol (69% increase in male mice) compared to wild-type mice
• increased circulating HDL cholesterol level (MGI Ref ID J:81821)
  • 57% increase in male compared to in wild-type mice
• increased circulating phospholipid level (MGI Ref ID J:81821)
  • over 50% increase compared to in wild-type mice

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Lipg^tm1Tq/Lipg^tm1Tq

        involves: 129S6/SvEvTac * C57BL/6J

• homeostasis/metabolism phenotype
• increased circulating cholesterol level (MGI Ref ID J:93987)
• increased circulating HDL cholesterol level (MGI Ref ID J:93987)
• increased circulating phospholipid level (MGI Ref ID J:93987)
• increased circulating triglyceride level (MGI Ref ID J:93987)

Lipg^tm1Tq/Lipg^tm1Tq

        involves: 129S6/SvEvTac

• homeostasis/metabolism phenotype
• abnormal circulating HDL cholesterol level (MGI Ref ID J:129942)
  • unlike in wild-type mice, HDL levels are not affected by hepatic proprotein convertase inhibition
• cardiovascular system phenotype
• abnormal vascular endothelial cell physiology (MGI Ref ID J:95158)
  • isolated aortic strips exhibit decreased monocyte binding compared to wild-type strips

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cardiovascular Research
Atherosclerosis
Hypercholesterolemia
Other (altered lipoprotein profile)

Cell Biology Research
Defects in Cell Adhesion Molecules

Genes & Alleles

Gene & Allele Information

Allele Symbol		Lipgtm1Tq
Allele Name		targeted mutation 1, Thomas Quertermous
Allele Type		Targeted (knock-out)
Common Name(s)		LIPG-;
Mutation Made By		Thomas Quertermous,   Stanford University School of Medicine
Strain of Origin		129S6/SvEvTac
ES Cell Line Name		TL1/TL-1
ES Cell Line Strain		129S6/SvEvTac
Gene Symbol and Name		Lipg, lipase, endothelial
Chromosome		18
Gene Common Name(s)		3110013K01Rik; EDL; EL; PRO719; RIKEN cDNA 3110013K01 gene; endothelial lipase; lipase; mEDL;
Molecular Note		The gene was disrupted by insertion of a neomycin resistance cassette into exon 1 via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by Northern blot analysis of lung, kidney, aorta, liver, and heart. [MGI Ref ID J:81821]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Ishida T; Choi S; Kundu RK; Hirata K; Rubin EM; Cooper AD; Quertermous T. 2003. Endothelial lipase is a major determinant of HDL level. J Clin Invest 111(3):347-55. [PubMed: 12569160]  [MGI Ref ID J:81821]

Additional References

Lipg^tm1Tq related

Ishida T; Choi SY; Kundu RK; Spin J; Yamashita T; Hirata K; Kojima Y; Yokoyama M; Cooper AD; Quertermous T. 2004. Endothelial lipase modulates susceptibility to atherosclerosis in apolipoprotein-E-deficient mice. J Biol Chem 279(43):45085-92. [PubMed: 15304490]  [MGI Ref ID J:93987]

Jin W; Wang X; Millar JS; Quertermous T; Rothblat GH; Glick JM; Rader DJ. 2007. Hepatic proprotein convertases modulate HDL metabolism. Cell Metab 6(2):129-36. [PubMed: 17681148]  [MGI Ref ID J:129942]

Kojma Y; Hirata K; Ishida T; Shimokawa Y; Inoue N; Kawashima S; Quertermous T; Yokoyama M. 2004. Endothelial lipase modulates monocyte adhesion to the vessel wall. A potential role in inflammation. J Biol Chem 279(52):54032-8. [PubMed: 15485805]  [MGI Ref ID J:95158]

Wang X; Jin W; Rader DJ. 2007. Upregulation of macrophage endothelial lipase by toll-like receptors 4 and 3 modulates macrophage interleukin-10 and -12 production. Circ Res 100(7):1008-15. [PubMed: 17347473]  [MGI Ref ID J:133915]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes, but heterozygous mice have greater fecundity.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.