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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 02-NOV-06 Species laboratory mouse Generation N13+N1F14 (30-NOV-11)
Generation DefinitionsDonating Investigator Dan Littman, New York University Medical Center Description
Mice homozygous for this EGFP "knock-in" are viable, fertile, normal in size, and do not display any behavioral abnormalities when maintained under barrier conditions. Lymph nodes and spleen show no endogenous gene expression. Lymphocytes from heterozygotes, but not homozygotes, show endogenous ligand binding. EGFP expression is restricted to spleen and lymph nodes, specifically activated/memory T cells, with a slightly higher intensity in homozygotes. Homozygous null mice show decreased EGFP+ CD1d-reactive NKT patrolling efficiency and decreased severity of induced acute hepatitis, while heterozygotes and wildtype mice show no differences. This mutant may be useful in studies of hepatitis, HIV, SIV, fluorescent T cell tracking, and various immune responses.Development
A targeting vector containing an enhanced green fluorescent protein (EGFP, Clonetech)/SV40 poly(A)n cassette with a downstream loxP-flanked neomycin resistance gene and herpes simplex virus thymidine kinase was used to replace the endogenous coding sequence. The construct was electroporated into the 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre recombinase-expressing vector before being injected into C57BL/6 blastocysts. The resulting chimeric mice were bred to C57BL/6 mice. The donating investigator stated that heterozygotes were backcrossed to C57BL/6 mice (see SNP note below) for 13 generations before being made homozygous.A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (345 strains)
005700 C.129P2-Cxcr6tm1Litt/J
005700 C.129P2-Cxcr6tm1Litt/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Cxcr6tm1Litt/Cxcr6tm1Litt
involves: 129P2/OlaHsd * C57BL/6
- normal phenotype
- no abnormal phenotype detected
- in a specific pathogen-free environment mice are viable with normal immune cell populations, T cell proliferation, and IgM and IgG1 levels (MGI Ref ID J:104242)
This mouse can be used to support research in many areas including:
GFP relatedImmunology and Inflammation Research
Mechanisms of HIV Infection
Research Tools
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Tissue/Cell Markers: T cell specific surface marker
Immunology and Inflammation Research
AIDS research tool
T cell specific surface marker
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Cxcr6tm1Litt | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Daniel Littman | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Bonzo-; Bz-; Cxcr6tm1Unu; cxcr6gfp; | ||
| Mutation Made By | Dan Littman, New York University Medical Center | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | EGFP expression mimics endogenous gene expression and is detected in the spleen and lymph nodes, specifically activated/memory T cells. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Molecular Note | The coding sequence was replaced with an EGFP. RT-PCR failed to detect transcript in mutants. Strong GFP expression was detected in spleen and lymphoid nodes. [MGI Ref ID J:104242] [MGI Ref ID J:131925] | ||
| Gene Symbol and Name | Cxcr6, chemokine (C-X-C motif) receptor 6 | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | BB217514; BONZO; CD186; STRL33; TYMSTR; expressed sequence BB217514; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Cxcr6tm1Litt, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Unutmaz D; Xiang W; Sunshine MJ; Campbell J; Butcher E; Littman DR. 2000. The primate lentiviral receptor Bonzo/STRL33 is coordinately regulated with CCR5 and its expression pattern is conserved between human and mouse. J Immunol 165(6):3284-92. [PubMed: 10975845] [MGI Ref ID J:104242]
Additional References
Geissmann F; Cameron TO; Sidobre S; Manlongat N; Kronenberg M; Briskin MJ; Dustin ML; Littman DR. 2005. Intravascular immune surveillance by CXCR6+ NKT cells patrolling liver sinusoids. PLoS Biol 3(4):e113. [PubMed: 15799695] [MGI Ref ID J:117341]
Cxcr6tm1Litt relatedCullen R; Germanov E; Shimaoka T; Johnston B. 2009. Enhanced tumor metastasis in response to blockade of the chemokine receptor CXCR6 is overcome by NKT cell activation. J Immunol 183(9):5807-15. [PubMed: 19812206] [MGI Ref ID J:156621]
Galkina E; Harry BL; Ludwig A; Liehn EA; Sanders JM; Bruce A; Weber C; Ley K. 2007. CXCR6 promotes atherosclerosis by supporting T-cell homing, interferon-gamma production, and macrophage accumulation in the aortic wall. Circulation 116(16):1801-11. [PubMed: 17909108] [MGI Ref ID J:139833]
Geissmann F; Cameron TO; Sidobre S; Manlongat N; Kronenberg M; Briskin MJ; Dustin ML; Littman DR. 2005. Intravascular immune surveillance by CXCR6+ NKT cells patrolling liver sinusoids. PLoS Biol 3(4):e113. [PubMed: 15799695] [MGI Ref ID J:117341]
Germanov E; Veinotte L; Cullen R; Chamberlain E; Butcher EC; Johnston B. 2008. Critical role for the chemokine receptor CXCR6 in homeostasis and activation of CD1d-restricted NKT cells. J Immunol 181(1):81-91. [PubMed: 18566372] [MGI Ref ID J:137250]
Johnston B; Kim CH; Soler D; Emoto M; Butcher EC. 2003. Differential chemokine responses and homing patterns of murine TCR alpha beta NKT cell subsets. J Immunol 171(6):2960-9. [PubMed: 12960320] [MGI Ref ID J:120380]
Matsumura S; Wang B; Kawashima N; Braunstein S; Badura M; Cameron TO; Babb JS; Schneider RJ; Formenti SC; Dustin ML; Demaria S. 2008. Radiation-induced CXCL16 release by breast cancer cells attracts effector T cells. J Immunol 181(5):3099-107. [PubMed: 18713980] [MGI Ref ID J:138960]
Monticelli LA; Yang Y; Knell J; D'Cruz LM; Cannarile MA; Engel I; Kronenberg M; Goldrath AW. 2009. Transcriptional regulator Id2 controls survival of hepatic NKT cells. Proc Natl Acad Sci U S A 106(46):19461-6. [PubMed: 19884494] [MGI Ref ID J:154768]
Velazquez P; Cameron TO; Kinjo Y; Nagarajan N; Kronenberg M; Dustin ML. 2008. Cutting edge: activation by innate cytokines or microbial antigens can cause arrest of natural killer T cell patrolling of liver sinusoids. J Immunol 180(4):2024-8. [PubMed: 18250405] [MGI Ref ID J:131925]
Wong CH; Jenne CN; Lee WY; Leger C; Kubes P. 2011. Functional innervation of hepatic iNKT cells is immunosuppressive following stroke. Science 334(6052):101-5. [PubMed: 21921158] [MGI Ref ID J:177038]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are maintained as homozygotes. Mating System Homozygote x Homozygote (Female x Male) 02-NOV-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $172.00 Female or Male Homozygous for Cxcr6tm1Litt
Pairs /Price (US dollars $) Pair Genotype $344.00 Homozygous for Cxcr6tm1Litt x Homozygous for Cxcr6tm1Litt Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $223.60 Female or Male Homozygous for Cxcr6tm1Litt
Pairs /Price (US dollars $) Pair Genotype $447.20 Homozygous for Cxcr6tm1Litt x Homozygous for Cxcr6tm1Litt Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
|
|
|
Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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