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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation ?+N1p (25-DEC-05)
Generation DefinitionsDonating Investigator Edward F Rosloniec, Veterans Affairs Medical Center Description
Transgenic mice are viable, fertile, and normal in size. This mutant carries a transgene for a fully functional bovine and mouse type II collagen (260-267) specific IAq-restricted T cell receptor. Flow cytometric analysis show 90% Tcrb-V8.3 expression of CD4+ splenocytes and greater than 95% expression in CD4+ lymphocytes. The transgene confers severe accelerated autoimmune arthritis following type II collagen (CII) immunization. Splenocytes from tolerized mice have altered activation and memory cell surface markers and high levels of Th2 cytokine secretion compared to control. Estradiol completely prevents collagen-induced arthritis (CIA). This mutant mouse may be useful in studies of CIA, rheumatoid arthritis, and T regulatory cells and may also serve as a source of T cells that mediate development of autoimmune diseases.Development
Transgenic constructs containing the mouse alpha and beta chain of the T cell receptor recognizing both bovine and mouse type II collagen (260-267) were cloned from the Tcra-V11.1,Tcra-J(J17) and Tcrb-V8.3,Tcrb-D(D1),Tcrb-J(J1.4) expressing I-Aq-restricted T cell hybridoma qCII85.33. The constructs were co-injected into pronuclei of (C57BL/6 x C3H)F1 eggs. PCR-positive mice (founder line 24) were backcrossed to DBA/1LacJ for 8 generations.
Control Information
| Control | ||
|---|---|---|
| 001140 DBA/1LacJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Tcra
View Strains carrying other alleles of Tcra (45 strains)
Phenotype
Phenotype Information
assigned by genotype
Tg(Tcra,Tcrb)24Efro/0
D1.Cg-Tg(Tcra,Tcrb)24Efro
- immune system phenotype
- abnormal T cell differentiation
- following CII-tolerization, transgenic T cells from the spleen show increased levels of activation markers CD69 and CD71, compared to OVA-tolerized transgenic mice eased in CII-tolerized cells (MGI Ref ID J:104243)
- decreased T cell proliferation
- 24 hours after exposure to 200 ug of CII tolerogen, T cells show a greatly reduced capacity to proliferate on CII stimulus compared to T cells from mice exposed to 200 ug of OVA; 48 hours after CII exposure, T cells are refractory to stimulation by CII peptide up to 130 uM (MGI Ref ID J:104243)
- after 72 hours, CII-tolerized cells can be stimulated by CII concentrations that are >2 logs higher than that needed to stimulate OVA-tolerized transgenic T cells (MGI Ref ID J:104243)
- suppressed proliferative response is observed up to 7 days post-CII treatment (MGI Ref ID J:104243)
- increased T cell proliferation
- when stimulated in vitro with CII (type II collagen) or a CII peptide, naive T cells show a vigorous proliferative response, while non-transgenic T cells do not (MGI Ref ID J:104243)
- proliferation of CII-tolerized T cells can be restored when cultured with CII and very high Il2 concentrations (MGI Ref ID J:104243)
- abnormal cytokine secretion
- after overnight CII stimulation, CII-treated T cells produce equivalent levels of Il2 as OVA-treated T cells, but by 48 hours after tolerization, the Il2 response is reduced (MGI Ref ID J:104243)
- by 48 hours after tolerance induction, production of Th2 cytokines like Il4 and Il10 are enhanced in T cells from CII-treated mice compared to those from OVA-treated mice (MGI Ref ID J:104243)
- IFNgamma production is elevated in cultures from CII-treated mice (MGI Ref ID J:104243)
- without CII peptide stimulation, no cytokines can be detected in cultures (MGI Ref ID J:104243)
- autoimmune response
- when immunized with an emulsion containing CII and CFA, mice develop strong T-cell dependent antibody response to the immunogen and the autoantigen, murine CII (MGI Ref ID J:104243)
- in arthritis incidence in transgenic mice; 100 % of mice tolerized with OVA develop arthritis by day 24 at an accelerated rate, but only 2 mice tolerized with CII develop disease by 42 days (MGI Ref ID J:104243)
- when mice are tolerized with 3 consecutive doses of 200 ug OVA or CII, no CII-tolerized mice develop arthritis, whereas all OVA-tolerized mice develop disease by day 13 (MGI Ref ID J:104243)
- increased autoantibody level
- levels of anti-CII antibody levels are higher than in controls littermates at day 19, but at day 34 levels are high in both groups of mice (MGI Ref ID J:104243)
- increased susceptibility to autoimmune disorder
- immunized mice develop severe accelerated autoimmune arthritis as early as 11 days after immunization with maximum incidence and severity by 35 days compared to non-transgenic littermates which develop arthritis around day 20 and maximum incidence by day 50 which is similar to that seen in wild-type DBA/1 mice (MGI Ref ID J:104243)
- however, no spontaneous arthritis has been observed in transgenic mice up to 9 months of age (MGI Ref ID J:104243)
- increased memory T cell number
- markers (CD44high and CD62low) for memory T cell phenotypes are increased in CII-tolerized cells (MGI Ref ID J:104243)
- hematopoietic system phenotype
- abnormal T cell differentiation
- following CII-tolerization, transgenic T cells from the spleen show increased levels of activation markers CD69 and CD71, compared to OVA-tolerized transgenic mice eased in CII-tolerized cells (MGI Ref ID J:104243)
- decreased T cell proliferation
- 24 hours after exposure to 200 ug of CII tolerogen, T cells show a greatly reduced capacity to proliferate on CII stimulus compared to T cells from mice exposed to 200 ug of OVA; 48 hours after CII exposure, T cells are refractory to stimulation by CII peptide up to 130 uM (MGI Ref ID J:104243)
- after 72 hours, CII-tolerized cells can be stimulated by CII concentrations that are >2 logs higher than that needed to stimulate OVA-tolerized transgenic T cells (MGI Ref ID J:104243)
- suppressed proliferative response is observed up to 7 days post-CII treatment (MGI Ref ID J:104243)
- increased T cell proliferation
- when stimulated in vitro with CII (type II collagen) or a CII peptide, naive T cells show a vigorous proliferative response, while non-transgenic T cells do not (MGI Ref ID J:104243)
- proliferation of CII-tolerized T cells can be restored when cultured with CII and very high Il2 concentrations (MGI Ref ID J:104243)
- increased memory T cell number
- markers (CD44high and CD62low) for memory T cell phenotypes are increased in CII-tolerized cells (MGI Ref ID J:104243)
- cellular phenotype
- abnormal T cell differentiation
- following CII-tolerization, transgenic T cells from the spleen show increased levels of activation markers CD69 and CD71, compared to OVA-tolerized transgenic mice eased in CII-tolerized cells (MGI Ref ID J:104243)
- decreased T cell proliferation
- 24 hours after exposure to 200 ug of CII tolerogen, T cells show a greatly reduced capacity to proliferate on CII stimulus compared to T cells from mice exposed to 200 ug of OVA; 48 hours after CII exposure, T cells are refractory to stimulation by CII peptide up to 130 uM (MGI Ref ID J:104243)
- after 72 hours, CII-tolerized cells can be stimulated by CII concentrations that are >2 logs higher than that needed to stimulate OVA-tolerized transgenic T cells (MGI Ref ID J:104243)
- suppressed proliferative response is observed up to 7 days post-CII treatment (MGI Ref ID J:104243)
- increased T cell proliferation
- when stimulated in vitro with CII (type II collagen) or a CII peptide, naive T cells show a vigorous proliferative response, while non-transgenic T cells do not (MGI Ref ID J:104243)
- proliferation of CII-tolerized T cells can be restored when cultured with CII and very high Il2 concentrations (MGI Ref ID J:104243)
This mouse can be used to support research in many areas including:
Immunology, Inflammation and Autoimmunity Research
Inflammation
collagen induced arthritis
Research Tools
Immunology and Inflammation Research
T Cell Receptor Transgenics
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Tcra,Tcrb)24Efro | ||
|---|---|---|---|
| Allele Name | transgene insertion 24, Edward F Rosloniec | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | qCII24; | ||
| Mutation Made By | Edward Rosloniec, Veterans Affairs Medical Center | ||
| Strain of Origin | (C57BL/6 x C3H)F1 | ||
| Expressed Gene | Tcra, T cell receptor alpha chain, mouse, laboratory | ||
| Expressed Gene | Tcrb, T cell receptor beta chain, mouse, laboratory | ||
| Promoter | Tcra, T cell receptor alpha chain, mouse, laboratory | ||
| Promoter | Tcrb, T cell receptor beta chain, mouse, laboratory | ||
| Molecular Note | Transgenic constructs containing the mouse alpha and beta chain of the T cell receptor recognizing both bovine and mouse type II collagen (260-267) were cloned from the Tcra-V11.1,Tcra-J(J17) and Tcrb-V8.3,Tcrb-D(D1),Tcrb-J(J1.4) expressing I-Aq-restricted T cell hybridoma qCII85.33. Line 24 was established. The transgenic T cell receptor is expressed on >90% of CD4+ T cells in the spleen and lymph nodes. >90% of alphabeta+ T cells in the thymus. [MGI Ref ID J:104243] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Tcra)24Efro, Standard PCR
Tg(Tcra,Tcrb)24Efro, QPCR
Tg(Tcrb)24Efro, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Brand DD; Myers LK; Whittington KB; Latham KA; Stuart JM; Kang AH; Rosloniec EF. 2002. Detection of early changes in autoimmune T cell phenotype and function following intravenous administration of type II collagen in a TCR-transgenic model. J Immunol 168(1):490-8. [PubMed: 11751997] [MGI Ref ID J:104243]
Additional References
Tg(Tcra,Tcrb)24Efro relatedDe Almeida DE; Ling S; Pi X; Hartmann-Scruggs AM; Pumpens P; Holoshitz J. 2010. Immune dysregulation by the rheumatoid arthritis shared epitope. J Immunol 185(3):1927-34. [PubMed: 20592276] [MGI Ref ID J:162460]
Holoshitz J; Liu Y; Fu J; Joseph J; Ling S; Colletta A; Sharma P; Begun D; Goldstein S; Taichman R. 2013. An HLA-DRB1-Coded Signal Transduction Ligand Facilitates Inflammatory Arthritis: A New Mechanism of Autoimmunity. J Immunol 190(1):48-57. [PubMed: 23180817] [MGI Ref ID J:190842]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are maintained by breeding transgenic positive littermates.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2450.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $1600.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3185.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Embryos
Price (US dollars $) Frozen Embryo $2080.00 Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryopreserved Embryos
Available to most shipping destinations1
This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.
1 Shipments cannot be made to Australia due to Australian government import restrictions.
2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 001140 DBA/1LacJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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