Strain Name:

B6.129S-Galr1tm1Tpi/J

Stock Number:

005695

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Availability:

Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Generation?+N1p (05-FEB-06)
Generation Definitions
 
Donating Investigator John Shine,   Garvan Institute of Medical Research

Description
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical abnormalities. No gene product (mRNA) is detected by RT-PCR analysis of mRNA from brain, spinal cord and uterus tissue. Although galanin receptor 1 targeted mutant mice on an incipient congenic B6.129S(N6) genetic background have been described to sometimes exhibit spontaneous seizures triggered by handling or bright light conditions, the donating investigator indicates that this mouse strain does not exhibit seizure behavior.

Development
A targeting vector containing neomycin resistance gene was used to disrupt part of exon 1. The construct was electroporated into 129S1/SvImJ derived W9.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice, and then backcrossed to the same for 13 generations.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Galr1
005809   B6.129P2-Galr1tm1Dgen/J
View Strains carrying other alleles of Galr1     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Galr1tm1Tpi/Galr1tm1Tpi

        involves: 129S1/Sv
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype
    • mutants respond normally with a decrease in food intake to intracerebroventricular injections of Galp   (MGI Ref ID J:91683)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Receptor Defects
      GABA receptor

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Galr1tm1Tpi
Allele Name targeted mutation 1, Tiina Pauline Iismaa
Allele Type Targeted (Null/Knockout)
Common Name(s) GAL-R1-; Galr1-;
Mutation Made By Arie Jacoby,   Victor Chang Cardiac Research Institute
Strain of Origin129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line NameW9.5/W95
ES Cell Line Strain129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name Galr1, galanin receptor 1
Chromosome 18
Gene Common Name(s) GALNR; GALNR1; Galnr1;
Molecular Note A neomycin selection cassette was inserted 780 bp into the endogenous locus via homologous recombination. Normal transcript was undetected in homozygous mutant mice by RT-PCR analysis. [MGI Ref ID J:80441] [MGI Ref ID J:92467]

Genotyping

Genotyping Information

Genotyping Protocols

Galr1tm1Tpi, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Jacoby AS; Hort YJ; Constantinescu G; Shine J; Iismaa TP. 2002. Critical role for GALR1 galanin receptor in galanin regulation of neuroendocrine function and seizure activity. Brain Res Mol Brain Res 107(2):195-200. [PubMed: 12487125]  [MGI Ref ID J:80441]

Additional References

Galr1tm1Tpi related

Badie-Mahdavi H; Lu X; Behrens MM; Bartfai T. 2005. Role of galanin receptor 1 and galanin receptor 2 activation in synaptic plasticity associated with 3',5'-cyclic AMP response element-binding protein phosphorylation in the dentate gyrus: studies with a galanin receptor 2 agonist and galanin receptor 1 knockout mice. Neuroscience 133(2):591-604. [PubMed: 15885916]  [MGI Ref ID J:104256]

Blakeman KH; Hao JX; Xu XJ; Jacoby AS; Shine J; Crawley JN; Iismaa T; Wiesenfeld-Hallin Z. 2003. Hyperalgesia and increased neuropathic pain-like response in mice lacking galanin receptor 1 receptors. Neuroscience 117(1):221-7. [PubMed: 12605908]  [MGI Ref ID J:109299]

Fetissov SO; Jacoby AS; Brumovsky PR; Shine J; Iismaa TP; Hokfelt T. 2003. Altered hippocampal expression of neuropeptides in seizure-prone GALR1 knockout mice. Epilepsia 44(8):1022-33. [PubMed: 12887433]  [MGI Ref ID J:103048]

Grass S; Jacoby AS; Iismaa TP; Crawley JN; Xu XJ; Wiesenfeld-Hallin Z. 2003. Flexor reflex excitability in mice lacking galanin receptor galanin-R1. Neurosci Lett 345(3):153-6. [PubMed: 12842279]  [MGI Ref ID J:107608]

Holmes A; Kinney JW; Wrenn CC; Li Q; Yang RJ; Ma L; Vishwanath J; Saavedra MC; Innerfield CE; Jacoby AS; Shine J; Iismaa TP; Crawley JN. 2003. Galanin GAL-R1 receptor null mutant mice display increased anxiety-like behavior specific to the elevated plus-maze. Neuropsychopharmacology 28(6):1031-44. [PubMed: 12700679]  [MGI Ref ID J:106211]

Holmes A; Li Q; Koenig EA; Gold E; Stephenson D; Yang RJ; Dreiling J; Sullivan T; Crawley JN. 2005. Phenotypic assessment of galanin overexpressing and galanin receptor R1 knockout mice in the tail suspension test for depression-related behavior. Psychopharmacology (Berl) 178(2-3):276-85. [PubMed: 15365683]  [MGI Ref ID J:110141]

Krasnow SM; Hohmann JG; Gragerov A; Clifton DK; Steiner RA. 2004. Analysis of the contribution of galanin receptors 1 and 2 to the central actions of galanin-like peptide. Neuroendocrinology 79(5):268-77. [PubMed: 15249737]  [MGI Ref ID J:91683]

Mahoney SA; Hosking R; Farrant S; Holmes FE; Jacoby AS; Shine J; Iismaa TP; Scott MK; Schmidt R; Wynick D. 2003. The second galanin receptor GalR2 plays a key role in neurite outgrowth from adult sensory neurons. J Neurosci 23(2):416-21. [PubMed: 12533601]  [MGI Ref ID J:81743]

Malkmus S; Lu X; Bartfai T; Yaksh TL; Hua XY. 2005. Increased hyperalgesia after tissue injury and faster recovery of allodynia after nerve injury in the GalR1 knockout mice. Neuropeptides 39(3):217-21. [PubMed: 15944015]  [MGI Ref ID J:101959]

Mazarati A; Lu X; Shinmei S; Badie-Mahdavi H; Bartfai T. 2004. Patterns of seizures, hippocampal injury and neurogenesis in three models of status epilepticus in galanin receptor type 1 (GalR1) knockout mice. Neuroscience 128(2):431-441. [PubMed: 15350653]  [MGI Ref ID J:92467]

McColl CD; Jacoby AS; Shine J; Iismaa TP; Bekkers JM. 2006. Galanin receptor-1 knockout mice exhibit spontaneous epilepsy, abnormal EEGs and altered inhibition in the hippocampus. Neuropharmacology 50(2):209-18. [PubMed: 16243364]  [MGI Ref ID J:112788]

Mitsukawa K; Lu X; Bartfai T. 2009. Bidirectional regulation of stress responses by galanin in mice: involvement of galanin receptor subtype 1. Neuroscience 160(4):837-46. [PubMed: 19272414]  [MGI Ref ID J:149757]

Page AJ; Slattery JA; Brierley SM; Jacoby AS; Blackshaw LA. 2007. Involvement of galanin receptors 1 and 2 in the modulation of mouse vagal afferent mechanosensitivity. J Physiol 583(Pt 2):675-84. [PubMed: 17627995]  [MGI Ref ID J:141050]

Potter EK; Smith-White MA. 2005. Galanin modulates cholinergic neurotransmission in the heart. Neuropeptides 39(3):345-8. [PubMed: 15944033]  [MGI Ref ID J:101940]

Wrenn CC; Kinney JW; Marriott LK; Holmes A; Harris AP; Saavedra MC; Starosta G; Innerfield CE; Jacoby AS; Shine J; Iismaa TP; Wenk GL; Crawley JN. 2004. Learning and memory performance in mice lacking the GAL-R1 subtype of galanin receptor. Eur J Neurosci 19(5):1384-96. [PubMed: 15016096]  [MGI Ref ID J:96740]

Zorrilla EP; Brennan M; Sabino V; Lu X; Bartfai T. 2007. Galanin type 1 receptor knockout mice show altered responses to high-fat diet and glucose challenge. Physiol Behav 91(5):479-85. [PubMed: 17223141]  [MGI Ref ID J:129613]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as homozygotes.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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