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Strain Name:

B6.129-Otx1tm4(cre)Asim/J

Stock Number:

005697

Availability:

Repository-Cryopreserved


General Terms and Conditions

Genes & Alleles   Otx1;   Otx1tm4(cre)Asim;   cre;


Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Specieslaboratory mouse
Donating Investigator Antonio Simeone,   King's College London
GenerationF8+N1p (22-JAN-06)

Strain Description
This strain expresses Cre recombinase from the targeted locus. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes have a perinatal lethal phenotype. Expression of the Cre recombinase gene (mRNA) under the control of the endogenous gene promoter, is detected in the lateral midbrain of embryonic day 10.5 aged embryos and in the presumptive alar-basal plate boundary of embryonic day 12.5 aged embryos, closely mimicking the endogenous gene expression pattern. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination is first detected at embryonic day 8.7. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study developmentally critical gene function in the brain.

Strain Development
A targeting vector containing Cre coding sequence and neomycin resistance genes was used to disrupt most of exon 1 and all of exon 2. The construct was electroporated into (129X1/SvJ x 129S1/Sv)-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were backcrossed to C57BL/6 for 8 generations.

Gene & Allele Details

Allele Symbol Otx1tm4(cre)Asim
Allele Name targeted mutation 4, Antonio Simeone
Common Name(s) Otx1cre;
Mutation Made By Flora Vaccarino,   Yale University
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl+
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Site of Expressionlateral midbrain of embryonic day 10.5 aged embryos and in the presumptive alar-basal plate boundary of embryonic day 12.5 aged embryos
Expressed Gene cre, cre recombinase, bacteriophage P1
Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence.
Gene Symbol and Name Otx1, orthodenticle homolog 1 (Drosophila)
Chromosome 11
Gene Common Name(s) A730044F23Rik; FLJ38361; Jackson waltzer; MGC15736; OTX1X; RIKEN cDNA A730044F23 gene; jv;
Molecular Note Exon 2 and part of exon 1 were deleted and replaced with a cre-neo cassette via homologous recombination, resulting in cre expression under Otx1 regulatory elements. [MGI Ref ID J:83110]

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Generic Cre

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as heterozygotes due to homozygous perinatal lethality.

Related Strains

Strains carrying other alleles of Otx1
000531   C57BL/6J-Otx1jv/J
View Strains carrying other alleles of Otx1     (1 strain)

Strains carrying other alleles of cre
008569   129-Alpltm1(cre)Nagy/J
003328   129-Tg(Prm-cre)58Og/J
004337   129.Cg-Foxg1tm1(cre)Skm/J
005989   129;FVB-Tg(PTH-cre)4167Slib/J
004302   129S1-Hprt1tm1(cre)Mnn/J
003960   129S6-Tg(Prnp-GFP/cre)1Blw/J
004146   B6.129-Tg(Pcp2-cre)2Mpin/J
006785   B6.129P2(C)-Cd19tm1(cre)Cgn/J
006084   B6.129P2(Cg)-Foxg1tm1(cre)Skm/J
004781   B6.129P2-Lyz2tm1(cre)Ifo/J
005623   B6.129S-Shhtm2(cre/ESR1)Cjt/J
006600   B6.129S1-Mnx1tm4(cre)Tmj/J
005628   B6.129S2-Emx1tm1(cre)Krj/J
003755   B6.129S4-Meox2tm1(cre)Sor/J
006878   B6.129S6-Taglntm2(cre)Yec/J
006054   B6.C-Tg(CMV-cre)1Cgn/J
006230   B6.Cg-Cebpatm1Dgt Tg(Mx1-cre)1Cgn/J
005622   B6.Cg-Shhtm1(EGFP/cre)Cjt/J
006149   B6.Cg-Tg(ACTA1-cre)79Jme/J
003574   B6.Cg-Tg(Alb-cre)21Mgn/J
006881   B6.Cg-Tg(Aqp2-cre)1Dek/J
004682   B6.Cg-Tg(CAG-cre/Esr1)5Amc/J
005359   B6.Cg-Tg(Camk2a-cre)T29-1Stl/J
006137   B6.Cg-Tg(Cdh5-cre)7Mlia/J
006368   B6.Cg-Tg(Cr2-cre)3Cgn/J
006663   B6.Cg-Tg(Eno2-cre)39Jme/J
005069   B6.Cg-Tg(Fabp4-cre)1Rev/J
003573   B6.Cg-Tg(Ins2-cre)25Mgn/J
008068   B6.Cg-Tg(Itgax-cre)1-1Reiz/J
003802   B6.Cg-Tg(Lck-cre)548Jxm/J
003556   B6.Cg-Tg(Mx1-cre)1Cgn/J
007742   B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J
005657   B6.Cg-Tg(Myh6-cre/Esr1)1Jmk/J
003771   B6.Cg-Tg(Nes-cre)1Kln/J
005975   B6.Cg-Tg(Plp1-cre/ESR1)3.16Pop/J
005584   B6.Cg-Tg(Prrx1-cre)1Cjt/J
003967   B6.Cg-Tg(Rbp3-cre)528Jxm/J
008454   B6.Cg-Tg(Sox2-cre)1Amc/J
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
003966   B6.Cg-Tg(Syn1-cre)671Jxm/J
004128   B6.Cg-Tg(Tek-cre)12Flv/J
007606   B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J
008085   B6.Cg-Tg(UBC-cre/ESR1)1Ejb/J
006234   B6.Cg-Tg(tetO-cre)1Jaw/J
006475   B6.FVB(129S4)-Tg(Ckmm-cre)5Khn/J
006451   B6.FVB(129X1)-Tg(Sim1-cre)1Lowl/J
006333   B6.FVB(Cg)-Tg(Neurog3-cre)C1Able/J
003724   B6.FVB-Tg(EIIa-cre)C5379Lmgd/J
004586   B6.SJL-Tg(Vil-cre)997Gum/J
005650   B6129-Tg(Myh6-cre/Esr1)1Jmk/J
003552   B6129-Tg(Wap-cre)11738Mam/J
004847   B6;129-Gt(ROSA)26Sortm1(cre/Esr1)Nat/J
005549   B6;129-Pax3tm1(cre)Joe/J
006668   B6;129P2-Omptm4(cre)Mom/MomJ
007001   B6;129S-Tg(UBC-cre/ESR1)1Ejb/J
006410   B6;129S6-Chattm1(cre)Lowl/J
003466   B6;D2-Tg(Sycp1-cre)4Min/J
003734   B6;FVB-Tg(GZMB-cre)1Jcb/J
006302   B6;SJL-Slc6a3tm1.1(cre)Bkmn/J
004426   B6;SJL-Tg(Cga-cre)3Sac/J
003554   B6;SJL-Tg(Col2a1-cre)1Bhr/J
005249   B6;SJL-Tg(Krt1-15-cre/PGR)22Cot/J
007610   B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J
007252   B6Ei.129S4-Tg(Prm-cre)58Og/EiJ
003465   BALB/c-Tg(CMV-cre)1Cgn/J
004126   C.129P2-Cd19tm1(cre)Cgn/J
005673   C.Cg-Tg(Mx1-cre)1Cgn/J
006244   C.Cg-Tg(tetO-cre)1Jaw/J
006474   C57BL/6-Tg(Grik4-cre)G32-4Stl/J
006888   C57BL/6-Tg(Zp3-cre)1Gwh/J
003394   C57BL/6-Tg(Zp3-cre)3Mrt/J
003651   C57BL/6-Tg(Zp3-cre)93Knw/J
007567   C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
006405   FVB-Tg(Ckmm-cre)5Khn/J
006774   FVB-Tg(Col2a1-cre/ESR1)KA3Smac/J
006954   FVB-Tg(Ddx4-cre)1Dcas/J
004600   FVB-Tg(GFAP-cre)25Mes/J
006364   FVB-Tg(Nr5a1-cre)2Lowl/J
006139   FVB.Cg-Tg(ACTA1-cre)79Jme/J
006297   FVB.Cg-Tg(Eno2-cre)39Jme/J
008244   FVB.Cg-Tg(tetO-cre)1Jaw/J
003376   FVB/N-Tg(ACTB-cre)2Mrt/J
003314   FVB/N-Tg(EIIa-cre)C5379Lmgd/J
006143   FVB/N-Tg(Thy1-cre)1Vln/J
003377   FVB/N-Tg(Zp3-cre)3Mrt/J
005732   NOD.Cg-Tg(Lck-cre)548Jxm/AchJ
004986   NOD/ShiLt-Tg(Ins2-cre)3Lt/Lt
003855   NOD/ShiLt-Tg(Ins2-cre)5Lt/LtJ
004987   NOD/ShiLt-Tg(Ins2-cre)6Lt/Lt
004192   STOCK Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy Tg(Mx1-cre)1Cgn/J
006677   STOCK Olfr151tm28Mom/MomJ
005936   STOCK Tg(ACTA1-cre)79Jme/J
007684   STOCK Tg(Atoh1-cre/ESR1)14Fsh/J
004453   STOCK Tg(CAG-cre/Esr1)5Amc/J
005105   STOCK Tg(Chx10-EGFP/cre-ALPP)2Clc/J
005938   STOCK Tg(Eno2-cre)39Jme/J
004692   STOCK Tg(Hoxb7-cre)13Amc/J
008122   STOCK Tg(Ins2-cre/Esr1)1Dam/J
004782   STOCK Tg(KRT14-cre)1Amc/J
005107   STOCK Tg(KRT14-cre/Esr1)20Efu/J
003551   STOCK Tg(MMTV-cre)1Mam/J
003553   STOCK Tg(MMTV-cre)4Mam/J
002527   STOCK Tg(Mx1-cre)1Cgn/J
002858   STOCK Tg(Nes-cre)1Wme/J
002859   STOCK Tg(Nes-cre)2Wme/J
005667   STOCK Tg(Neurog3-cre)C1Able/J
008119   STOCK Tg(Neurog3-cre/Esr1)1Dam/J
006207   STOCK Tg(Pcp2-cre)1Amc/J
005965   STOCK Tg(Pomc1-cre)16Lowl/J
006395   STOCK Tg(Sim1-cre)1Lowl/J
004783   STOCK Tg(Sox2-cre)1Amc/J
004746   STOCK Tg(Tagln-cre)1Her/J
003829   STOCK Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J
002471   STOCK Tg(hCMV-cre)140Sau/J
006224   STOCK Tg(tetO-cre)1Jaw/J
View Strains carrying other alleles of cre     (115 strains)

Additional Web Information

Congenic Nomenclature
Cre-lox or FLP-FRT Systems

Research Applications

This mouse can be used to support research in many areas including:

Developmental Biology Research
Neurodevelopmental Defects
Perinatal Lethality (Homozygous)

Neurobiology Research
Cre-lox System (Cre Recombinase expression in neural tissue)
Neurodevelopmental Defects

Research Tools
Cre-lox System (Cre Recombinase Expression)
Developmental Biology Research (Cre-lox System)
Neurobiology Research

cre related

Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)

References

Selected Reference(s)

Puelles E; Acampora D; Lacroix E; Signore M; Annino A; Tuorto F; Filosa S; Corte G; Wurst W; Ang SL; Simeone A. 2003. Otx dose-dependent integrated control of antero-posterior and dorso-ventral patterning of midbrain. Nat Neurosci 6(5):453-60. [PubMed: 12652306]  [MGI Ref ID J:83110]

Additional References

Price and Supply Information

Strain Name: B6.129-Otx1tm4(cre)Asim/J
Stock Number: 005697

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.

LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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