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Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation F8+N1p (22-JAN-06) Donating Investigator Antonio Simeone, King's College London Description
This strain expresses Cre recombinase from the targeted locus. Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes have a perinatal lethal phenotype. Expression of the Cre recombinase gene (mRNA) under the control of the endogenous gene promoter, is detected in the lateral midbrain of embryonic day 10.5 aged embryos and in the presumptive alar-basal plate boundary of embryonic day 12.5 aged embryos, closely mimicking the endogenous gene expression pattern. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination is first detected at embryonic day 8.7. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful to study developmentally critical gene function in the brain.Development
A targeting vector containing Cre coding sequence and neomycin resistance genes was used to disrupt most of exon 1 and all of exon 2. The construct was electroporated into (129X1/SvJ x 129S1/Sv)-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric animals were backcrossed to C57BL/6 for 8 generations.
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Strains carrying other alleles of Otx1
000531 C57BL/6J-Otx1jv/GrsrJ View Strains carrying other alleles of Otx1 (1 strain)
Strains carrying other alleles of cre
View Strains carrying other alleles of cre (162 strains)
Introduction to Cre-lox technology
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:
cre relatedDevelopmental Biology Research
Neurodevelopmental Defects
Perinatal Lethality
Homozygous
Neurobiology Research
Cre-lox System
Cre Recombinase expression in neural tissue
Neurodevelopmental Defects
Research Tools
Cre-lox System
Cre Recombinase Expression
Developmental Biology Research
Cre-lox System
Neurobiology Research
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis: Cre-lox System
| Allele Symbol | Otx1tm4(cre)Asim | ||
|---|---|---|---|
| Allele Name | targeted mutation 4, Antonio Simeone | ||
| Allele Type | Targeted (knock-in) | ||
| Common Name(s) | Otx1cre; | ||
| Mutation Made By | Flora Vaccarino, Yale University | ||
| Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| ES Cell Line Name | R1 | ||
| ES Cell Line Strain | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> | ||
| Site of Expression | lateral midbrain of embryonic day 10.5 aged embryos and in the presumptive alar-basal plate boundary of embryonic day 12.5 aged embryos | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Gene Symbol and Name | Otx1, orthodenticle homolog 1 (Drosophila) | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | A730044F23Rik; FLJ38361; Jackson waltzer; MGC15736; OTX1X; RIKEN cDNA A730044F23 gene; jv; | ||
| Driver Note | Otx1 | ||
| Molecular Note | Exon 2 and part of exon 1 were deleted and replaced with a cre-neo cassette via homologous recombination, resulting in cre expression under Otx1 regulatory elements. [MGI Ref ID J:83110] | ||
Genotyping Protocols
Generic Cre Melt Curve Analysis, Melt Curve Analysis
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
Puelles E; Acampora D; Lacroix E; Signore M; Annino A; Tuorto F; Filosa S; Corte G; Wurst W; Ang SL; Simeone A. 2003. Otx dose-dependent integrated control of antero-posterior and dorso-ventral patterning of midbrain. Nat Neurosci 6(5):453-60. [PubMed: 12652306] [MGI Ref ID J:83110]
Otx1tm4(cre)Asim relatedHwang CH; Simeone A; Lai E; Wu DK. 2009. Foxg1 is required for proper separation and formation of sensory cristae during inner ear development. Dev Dyn 238(11):2725-34. [PubMed: 19842177] [MGI Ref ID J:153730]
Puelles E; Acampora D; Gogoi R; Tuorto F; Papalia A; Guillemot F; Ang SL; Simeone A. 2006. Otx2 controls identity and fate of glutamatergic progenitors of the thalamus by repressing GABAergic differentiation. J Neurosci 26(22):5955-64. [PubMed: 16738237] [MGI Ref ID J:109120]
Puelles E; Annino A; Tuorto F; Usiello A; Acampora D; Czerny T; Brodski C; Ang SL; Wurst W; Simeone A. 2004. Otx2 regulates the extent, identity and fate of neuronal progenitor domains in the ventral midbrain. Development 131(9):2037-48. [PubMed: 15105370] [MGI Ref ID J:89367]
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as heterozygotes due to homozygous perinatal lethality.
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Purchasing Information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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