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Strain Name:

STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J

Stock Number:

005699

Availability:

Repository-Cryopreserved

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Genes & Alleles   GFP;   Gnat2;   Gnat2cpfl3;   Pdx1;   Tg(tetO-Ipf1,EGFP)956.6Macd;   tetO;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator Raymond MacDonald,   UTSW Medical Center

Important Note
This strain is homozygous for Gnat2cpfl3, cone photoreceptor function loss 3, which affects bright light (photopic) vision.

Strain Description
Mice hemizygous for the transgene are viable, fertile, normal in size, and do not display any behavioral abnormalities. At the Jackson Laboratory, no homozygous mice were produced from mating hemizygotes together, suggesting that homozygous transgenic animals die in utero. Expression of the bicistronic transgene is under the regulation of a tetracycline promoter element (TRE; tetO). By themselves, these transgenic mice do not express EGFP, but when these mice are mated with a second transgenic strain expressing tetracycline-transactivator (tTA) protein under the control of a tissue-specific promoter, Ipf1 and EGFP are highly expressed in the appropriate tissue of bitransgenic offspring.

This mouse was originally designed to be mated to an apancreatic targeted mutant with tTAoff in place of the endogenous Ipf1 gene (see Stock No. 005701). The combined mutations allow normal pancreatic development and function until doxycycline treatment renders the double mutant mouse conditionally null for Ipf1 expression. Using these double transgenic mice, pancreatic developmental can be arrested at any stage during embryonic development or in adult mice. Such double mutant mice may be useful in studies of pancreatic endocrine/exocrine function and diabetes. Tg(tetO-Ipf1,EGFP)956.6Macd transgenic mice also may be bred with other tTA transgenic strains for conditional mutation analysis.

Strain Development
A bicistronic transgenic vector was generated containing an Ipf1 minigene (both exons linked with a shortened intron and preceded by a truncated 5' untranslated region) and an internal ribosome entry site-linked enhanced green fluorescent protein (EGFP) all under transcriptional control of a tetracycline-responsive regulatory element (TRE). Transgenic mice were generated by pronuclear injection of the construct into fertilized [C57BL/6 x SJL] F2 hybrid embryos. Two-cell stage eggs were implanted into pseudopregnant foster mothers. Founder line 956.6 was obtained and was maintained by transgenic positive sibling intercross. At some point, transgenic mice were bred to B6;129 mice with a targeted mutation. The targeted mutation was selected against in subsequent breedings, and the transgenic line was again maintained by hemizygous intercrosses prior to cryopreservation.

Mammalian Phenotype Terms assigned by genotype

The following phenotype relates to a compound genotype created using this strain.
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Pdx1tm1Macd/Pdx1tm1Macd Tg(tetO-Ipf1,EGFP)956.6Macd/0

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL
  • homeostasis/metabolism phenotype
  • decreased circulating glucose level (MGI Ref ID J:101742)
    • 14 days after doxycycline withdrawal after 14 days of treatment resulted in a significant decrease in blood glucose levels with 4/5 mice becoming normoglycemic by 28 days
  • impaired glucose tolerance (MGI Ref ID J:79206)
    • adult transgenic rescue animals treated with doxycycline for 14 days exhibit a defective response to glucose challenge compared to wild type, non mutant transgenic, or untreated rescue mice
    • when adult mice are treated with doxycycline for 0, 7, 14, or 21 days, mice show an increased early glucose response within 7 days of start of treatment and ability to recover from glucose challenge is impaired compared to untreated transgenics where glucose levels recover to basal levels within 3 hours; by 14 days of doxycycline treatment, mice have diabetes
  • increased circulating glucose level (MGI Ref ID J:79206)
    • with doxycycline treatment for 21 day, adult rescue mice show a 4-fold increase in fasting blood glucose to diabetic levels
  • endocrine/exocrine gland phenotype
  • abnormal islet of Langerhans morphology (MGI Ref ID J:101742)
    • after dox treatment for 14 days, there is a significant decrease in islet area compared to wild type
    • abnormal pancreatic beta cell morphology (MGI Ref ID J:101742)
      • after 14 or 28 days of dox treatment there are, still present, beta cells which lack insulin
  • abnormal pancreas development (MGI Ref ID J:79206)
    • pancreas of transgenic rescue newborn mice is 50% the size of a normal wild type neonatal pancreas
    • treatment of pregnant mice with doxycycline from the first day of pregnancy through parturition prevents formation of the pancreas in mice with the transgenic rescue genotype
    • treatment on E11.5 arrests pancreatic development ~36 hours later; at birth the underdeveloped remnant consisted of a large and extended duct with several terminal, aborted, ductal buds with ducts lined with a single layer of primitive epithelial cells and with no acinar or islet tissue
  • decreased insulin secretion (MGI Ref ID J:101742)
    • there is a marked reduction in insulin in pancreata of doxycycline-treated mice; after withdrawal of doxycycline, insulin +ve cells are detected in islets of smaller islet-like cell clusters
  • increased glucagon secretion (MGI Ref ID J:101742)
    • in doxycycline-treated mice there is an increase in glucagons-positive cells
  • digestive/alimentary phenotype
  • abnormal islet of Langerhans morphology (MGI Ref ID J:101742)
    • after dox treatment for 14 days, there is a significant decrease in islet area compared to wild type
    • abnormal pancreatic beta cell morphology (MGI Ref ID J:101742)
      • after 14 or 28 days of dox treatment there are, still present, beta cells which lack insulin
  • abnormal pancreas development (MGI Ref ID J:79206)
    • pancreas of transgenic rescue newborn mice is 50% the size of a normal wild type neonatal pancreas
    • treatment of pregnant mice with doxycycline from the first day of pregnancy through parturition prevents formation of the pancreas in mice with the transgenic rescue genotype
    • treatment on E11.5 arrests pancreatic development ~36 hours later; at birth the underdeveloped remnant consisted of a large and extended duct with several terminal, aborted, ductal buds with ducts lined with a single layer of primitive epithelial cells and with no acinar or islet tissue
  • decreased insulin secretion (MGI Ref ID J:101742)
    • there is a marked reduction in insulin in pancreata of doxycycline-treated mice; after withdrawal of doxycycline, insulin +ve cells are detected in islets of smaller islet-like cell clusters
  • increased glucagon secretion (MGI Ref ID J:101742)
    • in doxycycline-treated mice there is an increase in glucagons-positive cells
  • cellular phenotype
  • increased cell proliferation (MGI Ref ID J:101742)
    • foci of duct proliferation are present in mice after 14 days of doxycycline treatment and become more prominent with continued treatment

Gene & Allele Details

Allele Symbol Tg(tetO-Ipf1,EGFP)956.6Macd
Allele Name transgene insertion 956-6, Raymond J MacDonald
Common Name(s) Pdx1-EGFP; Pdx1-GFP; Pdx1Tg; TgPdx1; tTA-responsive Pdx1-EGFP; tetO-Pdx1; tetO-Pdx1-EGFP; tetO-Pdx1/EGFP; tetO/Pdx1/EGFP;
Mutation Made By Raymond MacDonald,   UTSW Medical Center
Strain of Origin(C57BL/6 x SJL)F2
Site of ExpressionTransgenic mice do not express EGFP until a tetracycline-transactivator (tTA) protein is introduced; after which point Ipf1 and EGFP from the transgene are highly expressed. Transgenic mice have low level transgene expression (mRNA) in liver, kidney, spleen, and salivary gland.
Expressed Gene Pdx1, pancreatic and duodenal homeobox 1, mouse, laboratory
Expressed Gene GFP, Green Fluorescent Protein, jellyfish
Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source.
Promoter tetO, tet operator,
Molecular Note A transgenic vector containing an Ipf1 minigene (both exons of Ipf1 linked by a shortened exon and preceded by a truncated 5' UTR and an IRES-linked EGFP reporter) under control of seven direct repeats of the tetracycline operator (tetO) fused to the human cytomegalovirus (hCMV) immediate early promoter. High expression of Ipf1 and EGFP is induced by introduction of the tetracycline-transactivator protein by breeding with an appropriate mouse line. [MGI Ref ID J:79206]
 
Allele Symbol Gnat2cpfl3
Allele Name cone photoreceptor function loss 3
Common Name(s) Gnat2;
Strain of Originvarious
Gene Symbol and Name Gnat2, guanine nucleotide binding protein, alpha transducing 2
Chromosome 3
Gene Common Name(s) ACHM4; AW490837; GNATC; Gnat-2; Gt-2; Tcalpha; expressed sequence AW490837;
General Note This allele has been detected in the following strains either by genotyping or complementation testing: ALS/LtJ, SENCARA/PtJ, SENCARB/PtJ, SENCARC/PtJ, PN/nBSwUmabJ. (J:122428)

Phenotypic Similarity to Human Syndrome in Orthologous Human Gene: OMIM +139340 ACHROMATOPSIA 4.

Molecular Note A single nucleotide substitution of G to A at position 598 in exon 6. This mutation converts codon 200 from apartic acid to asparagine. [MGI Ref ID J:122428]

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls
  Control Pricing Information for JAX® GEMM® Strains

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, hemizygous mice are bred to wildtype siblings. When mating hemizygous mice together at The Jackson Laboratory, none of the 48 pups sampled were homozygous, indicating that homozygotes are not viable.

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006067   129-Gt(ROSA)26Sortm2Luo/J
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003960   129S6-Tg(Prnp-GFP/cre)1Blw/J
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004178   B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J
004218   B6.129(ICR)-Tg(CAG-ECFP)CK6Nagy/J
006071   B6.129-Gt(ROSA)26Sortm1Luo/J
006080   B6.129-Gt(ROSA)26Sortm2Luo/J
006075   B6.129-Gt(ROSA)26Sortm3Luo/J
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005582   B6.129P-Cx3cr1tm1Litt/J
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005622   B6.Cg-Shhtm1(EGFP/cre)Cjt/J
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006055   B6.Cg-Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
006051   B6.Cg-Tg(CAG-DsRed*MST)1Nagy/J
005884   B6.Cg-Tg(CAG-mRFP1)1F1Hadj/J
006069   B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J
005029   B6.Cg-Tg(Hlxb9-GFP)1Tmj/J
006098   B6.Cg-Tg(Il2/NFAT-luc)83Rinc/J
006864   B6.Cg-Tg(Ins1-EGFP)1Hara/J
005244   B6.Cg-Tg(Krt1-15-EGFP)2Cot/J
007742   B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J
007902   B6.Cg-Tg(RP23-268L19-EGFP)2Mik/J
007894   B6.Cg-Tg(Rgs4-EGFP)4Lvt/J
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
004659   B6.Cg-Tg(TIE2GFP)287Sato/1J
006101   B6.Cg-Tg(TRE/Prl-luc)31FlvRinc/J
007901   B6.Cg-Tg(Thy1-Brainbow1.0)HLich/J
007911   B6.Cg-Tg(Thy1-Brainbow1.1)MLich/J
007921   B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J
003710   B6.Cg-Tg(Thy1-CFP)23Jrs/J
007940   B6.Cg-Tg(Thy1-CFP/COX8A)C1Lich/J
007612   B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J
007615   B6.Cg-Tg(Thy1-COP4/EYFP)9Gfng/J
005630   B6.Cg-Tg(Thy1-EYFP)15Jrs/J
003709   B6.Cg-Tg(Thy1-YFP)16Jrs/J
005627   B6.Cg-Tg(Thy1-YFP/Syp)10Jrs/J
003782   B6.Cg-Tg(Thy1-YFPH)2Jrs/J
007606   B6.Cg-Tg(Thy1-cre/ESR1,-EYFP)AGfng/J
006000   B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
004509   B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
006417   B6.FVB-Tg(Npy-hrGFP)1Lowl/J
005738   B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J
004077   B6;129-Gt(ROSA)26Sortm2Sho/J
008295   B6;129-Syt9tm1Sud/J
006676   B6;129P2-Olfr151tm26Mom/MomJ
006667   B6;129P2-Omptm3Mom/MomJ
004858   B6;129S1-Tshrtm1Rmar/J
007843   B6;129S4-Efnb2tm2Sor/J
008214   B6;129S4-Pou5f1tm2Jae/J
006614   B6;CB-Tg(Thy1-CFP/COX8A)C1Lich/J
006617   B6;CB-Tg(Thy1-CFP/COX8A)S2Lich/J
004966   B6;CBA-Tg(Acrv1-EGFP)2727Redd/J
004654   B6;CBA-Tg(Pou5f1-EGFP)2Mnn/J
007910   B6;CBA-Tg(Thy1-Brainbow1.0)LLich/J
005621   B6;D2-Tg(S100B-EGFP)1Wjt/J
005620   B6;D2-Tg(S100B-EYFP)1Wjt/J
004690   B6;FVB-Tg(Pcp2-EGFP)2Yuza/J
006147   B6;FVB-Tg(Sfpi1,-EGFP)7Dgt/J
006043   B6;SJL-Tg(Oxt/EGFP)AI03Wsy/J
008004   B6;SJL-Tg(Thy1-ECFP/VAMP2)1Sud/J
007610   B6;SJL-Tg(Thy1-cre/ESR1,-EYFP)VGfng/J
007880   B6SJL-Tg(Thy1-Stx1a/EYFP)1Sud/J
007856   B6SJL-Tg(Thy1-Syt1/ECFP)1Sud/J
004190   C.129-Il4tm1Lky/J
005700   C.129P2-Cxcr6tm1Litt/J
006769   C.Cg-Foxp3tm2Tch/J
004512   C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
008242   C57BL/6-Gt(ROSA)26Sortm1(Ikbkb)Rsky/J
006567   C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ
003291   C57BL/6-Tg(CAG-EGFP)1Osb/J
005070   C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J
004353   C57BL/6-Tg(UBC-GFP)30Scha/J
005706   C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
006618   C57BL/6-Tg(tetO-COX8A/EYFP)1Ksn/J
006362   C57BL/6J-Tg(CMV-Cox8a/EYFP)17J/J
007860   C57BL/6J-Tg(Eno2-YFP/Cox8a)ZRwb/J
007567   C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
003927   C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ
007677   CB6-Tg(Gad1-EGFP)G42Zjh/J
007076   CByJ.B6-Tg(UBC-GFP)30Scha/J
003718   FVB-Tg(GadGFP)45704Swn/J
005515   FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
006421   FVB-Tg(Pomc1-hrGFP)1Lowl/J
005125   FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J
006206   FVB.129S6-Gt(ROSA)26Sortm1(HIF1A/luc)Kael/J
003516   FVB.Cg-Tg(CAG-EGFP)B5Nagy/J
007483   FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
003257   FVB/N-Tg(GFAPGFP)14Mes/J
006698   NOD.Cg-Il4tm1Lky/JbsJ
008173   NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ
005076   NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ
008549   NOD.FVB-Tg(Itgax-DTR/EGFP)57Lan/JdkJ
005082   NOD/ShiLt-Tg(ACTB-Ica1/EGFP)18Mdos/MdosJ
005328   NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J
005334   NOD/ShiLt-Tg(Cd4-EGFP)1Lt/J
005282   NOD/ShiLtJ-Tg(Ins1-EGFP/GH1)14Hara/HaraJ
006331   STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
005130   STOCK Gt(ROSA)26Sortm1(Smo/EYFP)Amc/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
004808   STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
004779   STOCK Mapttm1(EGFP)Klt/J
006741   STOCK Olfr160tm1Mom Tg(Olfr151,taulacZ)BMom/MomJ
006678   STOCK Olfr160tm6Mom/MomJ
006669   STOCK Olfr17tm7Mom/MomJ
006570   STOCK Smn1tm1Msd Tg(Hlxb9-GFP)1Tmj Tg(SMN2)89Ahmb/J
007879   STOCK Stx1atm2Sud/J
005438   STOCK Tg(CAG-Bgeo,-DsRed*MST)1Nagy/J
006850   STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
006876   STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J
003920   STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
005441   STOCK Tg(CAG-DsRed*MST)1Nagy/J
003773   STOCK Tg(CAG-ECFP)CK6Nagy/J
003115   STOCK Tg(CAG-EGFP)B5Nagy/J
003116   STOCK Tg(CAG-EGFP)D4Nagy/J
005645   STOCK Tg(CAG-mRFP1)1F1Hadj/J
005105   STOCK Tg(Chx10-EGFP/cre-ALPP)2Clc/J
005854   STOCK Tg(Cp-EGFP)25Gaia/J
008241   STOCK Tg(Cspg4-DsRed.T1)1Akik/J
006334   STOCK Tg(Gad1-EGFP)94Agmo/J
006340   STOCK Tg(Gad1-EGFP)98Agmo/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
005418   STOCK Tg(HIST1H2BB/EGFP)1Pa/J
006866   STOCK Tg(Ins1-DsRed*T4)32Hara/J
006784   STOCK Tg(Ins1-ECFP)24Hara/J
003658   STOCK Tg(TIE2GFP)287Sato/J
006129   STOCK Tg(Zp3-EGFP)1Dean/J
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View Fluorescent Protein Strains     (145 strains)

Strains carrying   Gnat2cpfl3 allele
002448   129S1/SvImJ
003072   ALS/LtJ
006180   CD10/JlsJ
005052   PN/nBSwUmabJ
002746   SENCARA/PtJ
002747   SENCARB/PtJ
002748   SENCARC/PtJ
006135   STOCK Sgk3fz-ica/McirJ
003773   STOCK Tg(CAG-ECFP)CK6Nagy/J
005645   STOCK Tg(CAG-mRFP1)1F1Hadj/J
004623   STOCK Tg(Fos-lacZ)34Efu/J
005667   STOCK Tg(Neurog3-cre)C1Able/J
003262   STOCK Tg(Trp53A135V)L3Ber/J
005104   STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
View Strains carrying   Gnat2cpfl3     (14 strains)

Strains carrying other alleles of GFP
006053   129-Gt(ROSA)26Sortm1Luo/J
006067   129-Gt(ROSA)26Sortm2Luo/J
006041   129-Gt(ROSA)26Sortm3Luo/J
003960   129S6-Tg(Prnp-GFP/cre)1Blw/J
007676   B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
004178   B6.129(Cg)-Tg(CAG-Bgeo/GFP)21Lbe/J
006071   B6.129-Gt(ROSA)26Sortm1Luo/J
006080   B6.129-Gt(ROSA)26Sortm2Luo/J
006075   B6.129-Gt(ROSA)26Sortm3Luo/J
008451   B6.129P(Cg)-Ptprca Cx3cr1tm1Litt/LittJ
005582   B6.129P-Cx3cr1tm1Litt/J
005670   B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
005622   B6.Cg-Shhtm1(EGFP/cre)Cjt/J
007484   B6.Cg-Tyrc-2J Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
007575   B6.Cg-Tg(CAG-Ngb,-EGFP)1Dgrn/J
008111   B6.Cg-Tg(CAG-Ub*G76V/GFP)1Dant/J
008112   B6.Cg-Tg(CAG-Ub*G76V/GFP)2Dant/J
006069   B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J
005029   B6.Cg-Tg(Hlxb9-GFP)1Tmj/J
006864   B6.Cg-Tg(Ins1-EGFP)1Hara/J
005244   B6.Cg-Tg(Krt1-15-EGFP)2Cot/J
007742   B6.Cg-Tg(Myh11-cre,-EGFP)2Mik/J
007902   B6.Cg-Tg(RP23-268L19-EGFP)2Mik/J
007894   B6.Cg-Tg(Rgs4-EGFP)4Lvt/J
006361   B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J
004659   B6.Cg-Tg(TIE2GFP)287Sato/1J
007921   B6.Cg-Tg(Thy1-Brainbow2.1)RLich/J
006000   B6.FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
004509   B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
006417   B6.FVB-Tg(Npy-hrGFP)1Lowl/J
005738   B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J
004077   B6;129-Gt(ROSA)26Sortm2Sho/J
006667   B6;129P2-Omptm3Mom/MomJ
008080   B6;C3-Tg(CAG-SAC/EGFP)35Rang/J
004966   B6;CBA-Tg(Acrv1-EGFP)2727Redd/J
004654   B6;CBA-Tg(Pou5f1-EGFP)2Mnn/J
005621   B6;D2-Tg(S100B-EGFP)1Wjt/J
004690   B6;FVB-Tg(Pcp2-EGFP)2Yuza/J
006147   B6;FVB-Tg(Sfpi1,-EGFP)7Dgt/J
006043   B6;SJL-Tg(Oxt/EGFP)AI03Wsy/J
004512   C.FVB-Tg(Itgax-DTR/EGFP)57Lan/J
006567   C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ
003291   C57BL/6-Tg(CAG-EGFP)1Osb/J
005070   C57BL/6-Tg(Csf1r-EGFP-NGFR/FKBP1A/TNFRSF6)2Bck/J
007265   C57BL/6-Tg(Sry-EGFP)92Ei Chr YAKR/J/EiJ
007264   C57BL/6-Tg(Sry-EGFP)92Ei Tg(Sry)4Ei Chr YPOS/EiJ
004353   C57BL/6-Tg(UBC-GFP)30Scha/J
005706   C57BL/6-Tg(tetO-CDK5R1/GFP)337Lht/J
007567   C57BL/6J-Tg(Itgax-cre,-EGFP)4097Ach/J
003927   C57BL/6J-Tg(Sry-EGFP)92Ei/EiJ
007677   CB6-Tg(Gad1-EGFP)G42Zjh/J
007076   CByJ.B6-Tg(UBC-GFP)30Scha/J
003718   FVB-Tg(GadGFP)45704Swn/J
005515   FVB-Tg(ITGAM-DTR/EGFP)34Lan/J
006421   FVB-Tg(Pomc1-hrGFP)1Lowl/J
003516   FVB.Cg-Tg(CAG-EGFP)B5Nagy/J
007483   FVB.Cg-Tg(Tyr)3412ARpw Tg(Sry-EGFP)92Ei/EiJ
003257   FVB/N-Tg(GFAPGFP)14Mes/J
008173   NOD.Cg-Tg(Ins1-EGFP)1Hara/QtngJ
005076   NOD.Cg-Tg(tetO-EGFP/FADD)1Doi/DoiJ
008549   NOD.FVB-Tg(Itgax-DTR/EGFP)57Lan/JdkJ
005082   NOD/ShiLt-Tg(ACTB-Ica1/EGFP)18Mdos/MdosJ
005334   NOD/ShiLt-Tg(Cd4-EGFP)1Lt/J
005282   NOD/ShiLtJ-Tg(Ins1-EGFP/GH1)14Hara/HaraJ
006331   STOCK Gt(ROSA)26Sortm1(DTA)Jpmb/J
005572   STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J
007576   STOCK Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J
006741   STOCK Olfr160tm1Mom Tg(Olfr151,taulacZ)BMom/MomJ
006770   STOCK Rag1tm1Mom Tg(TIE2GFP)287Sato/J
006570   STOCK Smn1tm1Msd Tg(Hlxb9-GFP)1Tmj Tg(SMN2)89Ahmb/J
006850   STOCK Tg(CAG-Bgeo,-NOTCH1,-EGFP)1Lbe/J
006876   STOCK Tg(CAG-Bgeo,-TEL/AML1,-EGFP)A6Lbe/J
003920   STOCK Tg(CAG-Bgeo/GFP)21Lbe/J
003115   STOCK Tg(CAG-EGFP)B5Nagy/J
003116   STOCK Tg(CAG-EGFP)D4Nagy/J
005105   STOCK Tg(Chx10-EGFP/cre-ALPP)2Clc/J
005854   STOCK Tg(Cp-EGFP)25Gaia/J
006334   STOCK Tg(Gad1-EGFP)94Agmo/J
006340   STOCK Tg(Gad1-EGFP)98Agmo/J
007896   STOCK Tg(Gt(ROSA)26Sor-EGFP)I1Able/J
005418   STOCK Tg(HIST1H2BB/EGFP)1Pa/J
003658   STOCK Tg(TIE2GFP)287Sato/J
006129   STOCK Tg(Zp3-EGFP)1Dean/J
005104   STOCK Tg(tetO-HIST1H2BJ/GFP)47Efu/J
View Strains carrying other alleles of GFP     (84 strains)

Strains carrying other alleles of Pdx1
005701   STOCK Pdx1tm1Macd/J
005728   STOCK Tg(tetO-Ipf1,lacZ)958.1Macd/J
View Strains carrying other alleles of Pdx1     (2 strains)

View Strains carrying other alleles of tetO     (28 strains)

Additional Web Information

Fluorescent Proteins/lacZ Systems
Tet Expression Systems

Research Applications

This mouse can be used to support research in many areas including:

Endocrine Deficiency Research
Pancreas Defects

Metabolism Research
Enzyme Deficiency (exocrine pancreatic insufficiency)

Research Tools
Endocrine Deficiency Research
Fluorescent Proteins
Genetics Research (Mutagenesis and Transgenesis: Tetop Tet System)
Genetics Research (Tissue/Cell Markers: multiple)
Genetics Research (Tissue/Cell Markers: pancreatic beta cells)
Tet Expression Systems (tTA/rtTA Responsive Strains)

GFP related

Research Tools
Fluorescent Proteins

References

Selected Reference(s)

Holland AM; Hale MA; Kagami H; Hammer RE; MacDonald RJ. 2002. Experimental control of pancreatic development and maintenance. Proc Natl Acad Sci U S A 99(19):12236-41. [PubMed: 12221286]  [MGI Ref ID J:79206]

Additional References

Price and Supply Information

Strain Name: STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
Stock Number: 005699

Price Details

IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:

*Pricing for Shipping Destination selected:

        USA, Canada and Mexico

Price(s) in US dollars ($)
Cryorecovery Fee $1900.00
Cryopreserved Embryos Fee $1600.00

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryopreserved Embryos
This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page.
Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.

LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.
View Control Pricing Information for JAX® Strains.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

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