Strain Name: |
C.129P2-Cxcr6tm1Litt/J |
|---|---|
Stock Number: |
005700 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Cxcr6; Cxcr6tm1Litt; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Dan Littman, New York University Medical Center Generation N12F?+N1p (04-DEC-05) Strain Description
Mice homozygous for this EGFP "knock-in" are viable, fertile, normal in size, and do not display any behavioral abnormalities when maintained under barrier conditions. Lymph nodes and spleen show no endogenous gene expression. Lymphocytes from heterozygotes, but not homozygotes, show endogenous ligand binding. EGFP expression is restricted to spleen and lymph nodes, specifically activated/memory T cells, with a slightly higher intensity in homozygotes. Homozygous null mice show decreased EGFP+ CD1d-reactive NKT patrolling efficiency and decreased severity of induced acute hepatitis, while heterozygotes and wildtype mice show no differences. This mutant may be useful in studies of hepatitis, HIV, SIV, fluorescent T cell tracking, and various immune responses.In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
Strain Development
A targeting vector containing an enhanced green fluorescent protein (EGFP)/SV40 poly(A)n cassette with a downstream loxP-flanked neomycin resistance gene and herpes simplex virus thymidine kinase was used to replace the endogenous coding sequence. The construct was electroporated into the 129P2/OlaHsd-derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre recombinase-expressing vector before being injected into C57BL/6 blastocysts. The resulting chimeric mice were bred to BALB/c mice. Heterozygotes were backcrossed to BALB/c mice for 12 generations before being made homozygous.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Cxcr6tm1Litt | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Daniel Littman | ||
| Common Name(s) | Bonzo-; Bz-; Cxcr6tm1Unu; cxcr6gfp; | ||
| Mutation Made By | Dan Littman, New York University Medical Center | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Cxcr6, chemokine (C-X-C motif) receptor 6 | ||
| Chromosome | 9 | ||
| Gene Common Name(s) | BB217514; BONZO; CD186; STRL33; TYMSTR; expressed sequence BB217514; | ||
| Molecular Note | The coding sequence was replaced with an EGFP. RT-PCR failed to detect transcript in mutants. Strong GFP expression was detected in spleen and lymphoid nodes. [J:104242] [J:131925] | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000651 BALB/cJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Cxcr6tm1Unu
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are maintained as homozygotes. |
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Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (135 strains)
005693 B6.129P2-Cxcr6tm1Litt/J
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Research Applications
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
Mechanisms of HIV Infection
Research Tools
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: T cell specific surface marker)
Immunology and Inflammation Research (AIDS research tool)
Immunology and Inflammation Research (T cell specific surface marker)
References
Selected Reference(s)
Additional ReferencesUnutmaz D; Xiang W; Sunshine MJ; Campbell J; Butcher E; Littman DR. 2000. The primate lentiviral receptor Bonzo/STRL33 is coordinately regulated with CCR5 and its expression pattern is conserved between human and mouse. J Immunol 165(6):3284-92. [PubMed: 10975845] [J:104242]
Price and Supply Information
| Strain Name: | C.129P2-Cxcr6tm1Litt/J |
| Stock Number: | 005700 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
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| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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