Strain Name: |
STOCK Rag1tm1Mom Tg(TIE2-lacZ)182Sato/J |
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Stock Number: |
005707 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Rag1; Rag1tm1Mom; Tek; lacZ; Tg(TIE2-lacZ)182Sato; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Brenda Coomber, University of Guelph Generation ?+F2p Strain Description
Mice homozygous for both the targeted mutation and the transgene are viable and fertile. The transgene beta-galactosidase is well expressed, reflects endogenous Tek/Tie2 activity, and is specific to the vascular endothelium. Mice homozygous for the Rag1 mutation are immunodeficient as they lack mature B and T lymphocytes. This double mutant mouse may be useful in studies of cancer, tumor-related angiogenesis, and as a recipient of xenografted tumors.Strain Development
The transgene consists of a beta-galactosidase reporter gene under the control of a modified murine endothelial-specific receptor tyrosine kinase (Tek/Tie2) promoter. Targeted mutation of recombination activating gene-1 (Rag1) resulted in a 1356 base pair deletion in the 5' end of the endogenous coding sequence and PGK-Neo cassette insertion. Double mutant mice were generated by crossing homozygous female B6.129S7-Rag1tm1Mom/J (see Stock No. 002216) with hemizygous male FVB/N-Tg(TIE2-lacZ)182Sato/J (see Stock No. 002856) mice. Female progeny were then mated to males homozygous for the Rag1 mutation to fix Rag1-deficiency. Subsequent sibling intercross resulted in presumed homozygosity of the transgene on chromosome X (via test crosses to wildtype). Other transgene insertion points on autosomes are likely.
Gene & Allele Details
| Allele Symbol | Rag1tm1Mom | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Peter Mombaerts | ||
| Common Name(s) | RAG-1-; RAG1null; Rag-; Rag-1KO; Rag1-; | ||
| Mutation Made By | Peter Mombaerts, Max Planck Institute of Biophysics | ||
| Strain of Origin | 129S7/SvEvBrd-Hprt1<+> | ||
| ES Cell Line Name | AB1 | ||
| ES Cell Line Strain | 129S7/SvEvBrd-Hprt1<+> | ||
| Gene Symbol and Name | Rag1, recombination activating gene 1 | ||
| Chromosome | 2 | ||
| Gene Common Name(s) | MGC43321; RNF74; Rag-1; | ||
| Molecular Note | A 1356 bp genomic fragment of the Rag1 gene, encoding the nuclear localization signal and the zinc-finger motif, was replaced by a neomycin cassette. A mutant transcript expressed from this allele was detected by Northern blot in bone marrow derived cell lines from homozygous mice. [J:1934] [J:96036] | ||
| Allele Symbol | Tg(TIE2-lacZ)182Sato | ||
| Allele Name | transgene insertion 182, Thomas N Sato | ||
| Common Name(s) | Tg(TIE2LacZ)182Sato; Tg182Sato; Tie2-lacZ; Tie2/LacZ; | ||
| Mutation Made By | Thomas Sato, Weill Medical College of Cornell Univ | ||
| Site of Expression | Vascular endothelial cells in embryos and adults. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | Tek, endothelial-specific receptor tyrosine kinase, mouse, laboratory | ||
| General Note | In embryonic and adult transgenic mice on an FVB/N background, LacZ is expressed specifically in vascular endothelial cells. | ||
| Molecular Note | The transgene consists of a mouse Tek promoter and enhancer element, a lacZ reporter gene, and an SV40 polyadenylation sequence. The enhancer element is a genomic fragment extending from 3' of exon 1 to 10 kb into intron 1. Expression was driven in endothelial cells. [J:39276] | ||
Control Information
| Control | ||
|---|---|---|
| None Available | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Rag1tm1Mom
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as homozygous for both the targeted mutation and the transgene. Specific pathogen free conditions are recommended as these mice are immunodeficient (lack mature B and T lymphocytes). Donating investigator reports occasional abnormalities at their facility; mice can agressively barber their cagemates, have tooth malocclusion at weaning, and a few develop 'crystal pneumonitis'. |
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Related Strains
lacZ Expression Strains
View lacZ Expression Strains (174 strains)
002856 FVB/N-Tg(TIE2-lacZ)182Sato/J
002194 B6;CBA-Tg(CD3E)26Cpt/J 002506 STOCK Tg(CD3E)26Cpt-Rag1tm1Mom/J
004659 B6.Cg-Tg(TIE2GFP)287Sato/1J 004128 B6.Cg-Tg(Tek-cre)12Flv/J 003658 STOCK Tg(TIE2GFP)287Sato/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Research Applications
This mouse can be used to support research in many areas including:
lacZ relatedCancer Research
Toxicology (B and T cell deficiency) (xenograft/transplant host)
Immunology and Inflammation Research
Immunodeficiency (B and T cell deficiency)
Research Tools
Cancer Research (B and T cell deficiency) (xenograft/transplant host)
Cancer Research (endothelial cell marker for neovascularization)
Cancer Research (xenograft/transplant host)
Cardiovascular Research (endothelial cell marker (lacZ) for neovascularization)
Cardiovascular Research (endothelial cell marker for neovascularization)
Genetics Research (Tissue/Cell Markers: endothelial cell markers for neovascularization)
Genetics Research (Tissue/Cell Markers: endothelial cells)
Immunology and Inflammation Research (T cell deficiency) (xenograft/transplant host)
Toxicology Research (B and T cell deficiency) (xenograft transplant host)
lacZ Expression
Rag1tm1Mom relatedResearch Tools
lacZ Expression
Cancer Research
Toxicology (B and T cell deficiency) (xenograft/transplant host)
Hematological Research
Immunological Defects (B and T cell deficiency)
Immunology and Inflammation Research
Immunodeficiency (B and T cell deficiency)
Inflammation (B and T cell deficiency)
T Cell Receptor Signaling Defects (B and T cell deficiency)
Internal/Organ Research
Lymphoid Tissue Defects (B and T cell deficiency)
Research Tools
Cancer Research (B and T cell deficiency) (xenograft/transplant host)
Toxicology Research (xenograft/transplant host)
References
Selected Reference(s)
Additional ReferencesFathers KE; Stone CM; Minhas K; Marriott JJ; Greenwood JD; Dumont DJ; Coomber BL. 2005. Heterogeneity of Tie2 expression in tumor microcirculation: influence of cancer type, implantation site, and response to therapy. Am J Pathol 167(6):1753-62. [PubMed: 16314485] [J:107320]
Price and Supply Information
| Strain Name: | STOCK Rag1tm1Mom Tg(TIE2-lacZ)182Sato/J |
| Stock Number: | 005707 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to the Supply Notes for further information. |
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| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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