Strain Name:

B6.129-Skitm1Cco/J

Stock Number:

005709

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN21p (25-DEC-05)
Generation Definitions
 
Donating Investigator Clemencia Colmenares,   The Cleveland Clinic Foundation

Description
This mutant was created on a mixed (129 x C57BL/6J) background. Greater than 80% of mice on this mixed background had severe neural tube closure defects (such as exencephaly) while less than 16% exhibited facial clefting. The penetrance of these phenotypes has segregated with further backcross to C57BL/6J. The congenic mutant described here was backcrossed 12-14 times to wildtype C57BL/6J. Mice homozygous for the targeted mutation die before or shortly after birth due to developmental defects. Null mice are distinguishable from wildtype and heterozygous littermates at embryonic day 8.5 (E8.5) with delayed, but complete, cranial neural tube closure (incomplete at the normal E9.5). Ninety percent of null mice alive at E18.5 exhibit facial clefting. Other highly penetrant phenotypes include flattened snout, depressed nasal bridge, excessive orbital separation, elongated subventricular zones, vestigial polydactyly, and malformations of the olfactory bulb, iris, and ventral septum. Heterozygotes are viable and fertile with timely cranial neural tube closure and a low incidence of facial clefting. This mouse may be useful in studies of facial cleft formation, 1p36 deletion syndrome, oncogene function, apoptosis, and neural tube and skeletal muscle defects.

Development
A targeting vector was generated from a 129-mouse genomic library and contained the first coding exon of the endogenous gene. A thymidine kinase-driven neomycin resistance cassette was used to disrupt this exon and generated a small deletion in the coding sequence. The construct was electroporated into 129P2/OlaHsd-derived E14.1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting chimeric mice were bred to C57BL/6J mates and then backcrossed to C57BL/6J for more than 20 generations. This strain is now maintained by mating heterozygotes; homozygotes have perinatal lethality.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Facebase: models
007664   129S-Efnb1tm1Sor/J
000646   A/J
000647   A/WySnJ
002619   B6.129-Tgfb3tm1Doe/J
007453   B6.129P2(Cg)-Dhcr7tm1Gst/J
010525   B6.129S-Notch2tm3Grid/J
010616   B6.129S1-Jag1tm1Grid/J
010546   B6.129S1-Jag2tm1Grid/J
010620   B6.129S1-Notch2tm1Grid/J
009387   B6.129S1-Osr1tm1Jian/J
009386   B6.129S1-Osr2tm1Jian/J
010621   B6.129S1-Snai1tm2.1Grid/J
010617   B6.129S1-Snai2tm1Grid/J
003865   B6.129S2-Itgavtm1Hyn/J
003755   B6.129S4-Meox2tm1(cre)Sor/J
016902   B6.129S5-Irf6Gt(OST398253)Lex/J
003336   B6.129S7-Cdkn1ctm1Sje/J
012843   B6.129X1(Cg)-Slc32a1tm1.1Bgc/J
000026   B6.C3-Gli3Xt-J/J
004275   B6.Cg-Fignfi/Frk
012844   B6.Cg-Gad1tm1.1Bgc/J
006382   B6;129-Casktm1Sud/J
002711   B6;129-Gabrb3tm1Geh/J
004293   B6;129-Shhtm2Amc/J
012603   B6;129-Tgfbr2tm1Karl/J
010618   B6;129S-Jag1tm2Grid/J
010686   B6;129S-Snai1tm2Grid/J
009389   B6;129S1-Bambitm1Jian/J
010619   B6;129S1-Lfngtm1Grid/J
010547   B6;129S1-Notch3tm1Grid/J
010544   B6;129S1-Notch4tm1Grid/J
010722   B6;129S1-Snai2tm2Grid/J
012463   B6;129S4-Foxd1tm1(GFP/cre)Amc/J
022358   B6;129S6-Rr23tm1Axvi/Mmjax
022359   B6;129S6-Rr24tm1Axvi/Mmjax
022360   B6;129S6-Rr25tm1Axvi/Mmjax
003277   B6;129S7-Acvr2atm1Zuk/J
002788   B6;129S7-Fsttm1Zuk/J
002990   B6;129S7-Inhbatm1Zuk/J
000523   B6By.Cg-Eh/J
000278   B6C3Fe a/a-Papss2bm Hps1ep Hps6ru/J
000515   B6CBACa Aw-J/A-SfnEr/J
001434   C3HeB/FeJ x STX/Le-Mc1rE-so Gli3Xt-J Zeb1Tw/J
000252   DC/LeJ
005057   FVB.129-Kcnj2tm1Swz/J
012655   FVB.A-Irf6clft1/BeiJ
013100   FVB.C-Prdm16csp1/J
017437   FVB/N-Ckap5TgTn(sb-cHS4,Tyr)2320F-1Ove/J
017438   FVB/N-MidnTg(Tyr)2261EOve/J
017609   FVB/N-Rr16Tn(sb-Tyr)1HCebOve/J
017598   FVB/N-Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove/J
017608   FVB/N-Skor2Tn(sb-Tyr)1799B.CA7BOve/J
017436   FVB/N-Tapt1TgTn(sb-cHS4,Tyr)2508GOve/J
016870   FVB/NJ-Ap2b1Tg(Tyr)427Ove/EtevJ
017434   FVB;B6-Cramp1lTgTn(sb-rtTA,Tyr)2447AOve/J
017594   FVB;B6-Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve/J
017435   FVB;B6-SlmapTn(sb-rtTA)2426B.SB4Ove/J
003318   STOCK Shhtm1Amc/J
003102   STOCK Tgfb2tm1Doe/J
018624   STOCK Tgfb3tm2(Tgfb1)Vk/J
008469   STOCK Wnt9btm1.2Amc/J
View Facebase: models     (61 strains)

Strains carrying   Skitm1Cco allele
009091   129-Skitm1Cco/J
009092   A.129P-Skitm1Cco/J
View Strains carrying   Skitm1Cco     (2 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Shprintzen-Goldberg Craniosynostosis Syndrome; SGS   (SKI)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Skitm1Cco/Ski+

        involves: 129P2/OlaHsd * C57BL/6
  • tumorigenesis
  • increased incidence of tumors by chemical induction
    • n addition to the phenotype observed for heterozygotes on a 129P2 and Black Swiss background, heterozygotes on this background display increased succeptibility for tumors when injected IP with 9,10-dimethyl-1,2-benzanthracene   (MGI Ref ID J:73349)
  • increased lymphoma incidence
    • the most frequent tumors to develop were malignant lymphomas   (MGI Ref ID J:73349)
  • cellular phenotype
  • increased cell proliferation
    • In addition to the phenotype observed for heterozygotes on a 129P2 and Black Swiss background, heterozygotes on this background display increased rate of proliferation for MEF in culture   (MGI Ref ID J:73349)
  • homeostasis/metabolism phenotype
  • increased incidence of tumors by chemical induction
    • n addition to the phenotype observed for heterozygotes on a 129P2 and Black Swiss background, heterozygotes on this background display increased succeptibility for tumors when injected IP with 9,10-dimethyl-1,2-benzanthracene   (MGI Ref ID J:73349)

Skitm1Cco/Ski+

        involves: 129P2/OlaHsd * Black Swiss
  • craniofacial phenotype
  • midline facial cleft
    • frontonasal clefting occasionally seen   (MGI Ref ID J:42573)
  • nervous system phenotype
  • abnormal neural tube morphology/development   (MGI Ref ID J:42573)
  • exencephaly
    • occasional exencephally seen   (MGI Ref ID J:42573)
  • embryogenesis phenotype
  • abnormal neural tube morphology/development   (MGI Ref ID J:42573)
  • growth/size/body phenotype
  • midline facial cleft
    • frontonasal clefting occasionally seen   (MGI Ref ID J:42573)

Skitm1Cco/Skitm1Cco

        involves: 129P2/OlaHsd * C57BL/6
  • mortality/aging
  • partial lethality throughout fetal growth and development
    • while the phenotype expressed on this background is essentially the same as that on a 129P2 and Black Swiss background, embryonic lethality was delayed to between E14.5 and E18.5 while the phenotype expressed on this background is essentially the same as that on a 129P2 and Black Swiss background, embryonic lethality was delayed to between E14.5 and E18.5   (MGI Ref ID J:42573)
  • craniofacial phenotype
  • midline facial cleft
    • While the phenotype expressed on this background is essentially the same as that on a 129P2 and Black Swiss background, as backcrossing to C57BL/6 continues the frequency of facial clefts increases   (MGI Ref ID J:75396)
  • muscle phenotype
  • muscle hypoplasia
    • While the phenotype expressed on this background is essentially the same as that on a 129P2 and Black Swiss background, on a C57BL/6 background 50-60% of homozygous mice were obviously skinny with flaccid limbs   (MGI Ref ID J:42573)
  • nervous system phenotype
  • exencephaly
    • While the phenotype expressed on this background is essentially the same as that on a 129P2 and Black Swiss background, continued backcrossing to C57BL/6 reduces frequency of exencephaly   (MGI Ref ID J:75396)
  • growth/size/body phenotype
  • midline facial cleft
    • While the phenotype expressed on this background is essentially the same as that on a 129P2 and Black Swiss background, as backcrossing to C57BL/6 continues the frequency of facial clefts increases   (MGI Ref ID J:75396)

Skitm1Cco/Skitm1Cco

        involves: 129P2/OlaHsd * Black Swiss
  • mortality/aging
  • complete perinatal lethality
    • homozygotes dead at birth   (MGI Ref ID J:42573)
    • E18.5 pups born live by cesarean died shortly after birth   (MGI Ref ID J:42573)
  • partial lethality throughout fetal growth and development
    • moderate   (MGI Ref ID J:42573)
    • occurs between E9.5 and E14.5   (MGI Ref ID J:42573)
  • cardiovascular system phenotype
  • hemorrhage
    • in E18.5 embryos delivered by cesarean, severe hemorrhage occurs from exposed brain mass   (MGI Ref ID J:42573)
  • cellular phenotype
  • increased cell proliferation
    • increased rate of proliferation for MEF in culture   (MGI Ref ID J:73349)
  • craniofacial phenotype
  • abnormal jaw morphology
    • abnormal square jaw   (MGI Ref ID J:42573)
    • short mandible
      • mandibular bone is short and thick   (MGI Ref ID J:42573)
  • abnormal neurocranium morphology   (MGI Ref ID J:42573)
    • abnormal presphenoid bone morphology
      • presphenoid is always absent or abnormal   (MGI Ref ID J:42573)
    • absent frontal bone
      • loss of frontal bones   (MGI Ref ID J:42573)
    • absent interparietal bone
      • loss of interparietal bones   (MGI Ref ID J:42573)
    • absent parietal bone
      • loss of parietal bones   (MGI Ref ID J:42573)
    • small basioccipital bone
      • basioccipital bones are small and malformed   (MGI Ref ID J:42573)
    • small basisphenoid bone
      • basisphenoid is small and malformed   (MGI Ref ID J:42573)
  • midline facial cleft
    • frontonasal clefting found in 15% of pups and E18.5 embryos   (MGI Ref ID J:42573)
  • shortened head
    • facial flattening   (MGI Ref ID J:42573)
  • embryogenesis phenotype
  • abnormal ectomesenchyme morphology
    • excessive apoptosis in craniofacial mesenchyme at E9.5 but not E10.5   (MGI Ref ID J:42573)
  • abnormal paraxial mesoderm morphology
    • mesenchyme adjacent to neural tube was somewhat disorganized   (MGI Ref ID J:42573)
  • open neural tube
    • defect at the level of the midbrain-forebrain boundary and extending forward and backward or in one or the other direction   (MGI Ref ID J:42573)
    • occurs in about 85% of embryos   (MGI Ref ID J:42573)
    • excessive apoptosis at E9.5 but not at E10.5   (MGI Ref ID J:42573)
    • no caudal neural tube defects, spina bifida or kinky tails   (MGI Ref ID J:42573)
  • growth/size/body phenotype
  • abnormal ectomesenchyme morphology
    • excessive apoptosis in craniofacial mesenchyme at E9.5 but not E10.5   (MGI Ref ID J:42573)
  • decreased fetal size
    • E18.5 pups delivered by cesarean were 10% smaller than normal   (MGI Ref ID J:42573)
  • midline facial cleft
    • frontonasal clefting found in 15% of pups and E18.5 embryos   (MGI Ref ID J:42573)
  • shortened head
    • facial flattening   (MGI Ref ID J:42573)
  • limbs/digits/tail phenotype
  • polydactyly
    • vestigial hexadactyly   (MGI Ref ID J:75396)
  • muscle phenotype
  • abnormal skeletal muscle morphology
    • increased space between muscle fibers   (MGI Ref ID J:42573)
    • abnormal skeletal muscle fiber morphology
      • fibers were shorter and somewhat disorganized   (MGI Ref ID J:42573)
      • decreased skeletal muscle fiber diameter
        • reduced diameter of muscle fibers   (MGI Ref ID J:42573)
    • decreased skeletal muscle mass   (MGI Ref ID J:42573)
  • muscle hypoplasia
    • on a Swiss Black background, 15% extremely emaciated and 20% somewhat skinny   (MGI Ref ID J:42573)
  • skeleton phenotype
  • abnormal cervical vertebrae morphology
    • first three cervical vertebrae affected   (MGI Ref ID J:42573)
    • abnormal cervical atlas morphology   (MGI Ref ID J:42573)
    • abnormal cervical axis morphology   (MGI Ref ID J:42573)
  • abnormal jaw morphology
    • abnormal square jaw   (MGI Ref ID J:42573)
    • short mandible
      • mandibular bone is short and thick   (MGI Ref ID J:42573)
  • abnormal neurocranium morphology   (MGI Ref ID J:42573)
    • abnormal presphenoid bone morphology
      • presphenoid is always absent or abnormal   (MGI Ref ID J:42573)
    • absent frontal bone
      • loss of frontal bones   (MGI Ref ID J:42573)
    • absent interparietal bone
      • loss of interparietal bones   (MGI Ref ID J:42573)
    • absent parietal bone
      • loss of parietal bones   (MGI Ref ID J:42573)
    • small basioccipital bone
      • basioccipital bones are small and malformed   (MGI Ref ID J:42573)
    • small basisphenoid bone
      • basisphenoid is small and malformed   (MGI Ref ID J:42573)
  • abnormal skeleton development   (MGI Ref ID J:75396)
  • abnormal spine curvature
    • abnormal curvature of the back, head and neck   (MGI Ref ID J:42573)
  • vertebral transformation
    • homeotic transformations unless facial clefting is present   (MGI Ref ID J:42573)
  • vision/eye phenotype
  • eyelids open at birth
    • homozygotes are born with their eyes open   (MGI Ref ID J:42573)
  • nervous system phenotype
  • abnormal forebrain morphology
    • ventral midline fusion of the forebrain   (MGI Ref ID J:75396)
    • abnormal olfactory bulb morphology
      • malformed olfactory bulb   (MGI Ref ID J:75396)
  • exencephaly
    • exposed brain mass normally sheared off in normal birth   (MGI Ref ID J:42573)
  • open neural tube
    • defect at the level of the midbrain-forebrain boundary and extending forward and backward or in one or the other direction   (MGI Ref ID J:42573)
    • occurs in about 85% of embryos   (MGI Ref ID J:42573)
    • excessive apoptosis at E9.5 but not at E10.5   (MGI Ref ID J:42573)
    • no caudal neural tube defects, spina bifida or kinky tails   (MGI Ref ID J:42573)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence
      Lymphomas
      Lymphomas: chemical induced

Developmental Biology Research
Craniofacial and Palate Defects
      cleft palate and cleft lip
      congenital cleft palate
Eye Defects
Neural Tube Defects
Perinatal Lethality
      Homozygous
Skeletal Defects
      polydactyly

Neurobiology Research
Myelination Defects
Neural Tube Defects

Sensorineural Research
Eye Defects
Olfactory Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Skitm1Cco
Allele Name targeted mutation 1, Clemencia Colmenares
Allele Type Targeted (Null/Knockout)
Common Name(s) ski-;
Mutation Made By Clemencia Colmenares,   The Cleveland Clinic Foundation
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14.1
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Ski, ski sarcoma viral oncogene homolog (avian)
Chromosome 4
Gene Common Name(s) 2310012I02Rik; 2610001A11Rik; AA062172; AA589460; BC004088; MGC:8300; RGD1565591; RIKEN cDNA 2310012I02 gene; RIKEN cDNA 2610001A11 gene; SGS; SKV; c-ski; cDNA sequence BC004088; expressed sequence AA062172; expressed sequence AA589460;
Molecular Note Insertion of a neomycin resistance cassette into the exon 1 generated a small deletion in the coding sequence. [MGI Ref ID J:42573]

Genotyping

Genotyping Information

Genotyping Protocols

Skitm1Cco, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Colmenares C; Heilstedt HA; Shaffer LG; Schwartz S; Berk M; Murray JC; Stavnezer E. 2002. Loss of the SKI proto-oncogene in individuals affected with 1p36 deletion syndrome is predicted by strain-dependent defects in Ski-/- mice. Nat Genet 30(1):106-9. [PubMed: 11731796]  [MGI Ref ID J:75396]

Additional References

Skitm1Cco related

Atanasoski S; Notterpek L; Lee HY; Castagner F; Young P; Ehrengruber MU; Meijer D; Sommer L; Stavnezer E; Colmenares C; Suter U. 2004. The protooncogene Ski controls Schwann cell proliferation and myelination. Neuron 43(4):499-511. [PubMed: 15312649]  [MGI Ref ID J:96530]

Baranek C; Dittrich M; Parthasarathy S; Bonnon CG; Britanova O; Lanshakov D; Boukhtouche F; Sommer JE; Colmenares C; Tarabykin V; Atanasoski S. 2012. Protooncogene Ski cooperates with the chromatin-remodeling factor Satb2 in specifying callosal neurons. Proc Natl Acad Sci U S A 109(9):3546-51. [PubMed: 22334647]  [MGI Ref ID J:182157]

Berk M; Desai SY; Heyman HC; Colmenares C. 1997. Mice lacking the ski proto-oncogene have defects in neurulation, craniofacial, patterning, and skeletal muscle development. Genes Dev 11(16):2029-39. [PubMed: 9284043]  [MGI Ref ID J:42573]

Dai P; Shinagawa T; Nomura T; Harada J; Kaul SC; Wadhwa R; Khan MM; Akimaru H; Sasaki H; Colmenares C; Ishii S. 2002. Ski is involved in transcriptional regulation by the repressor and full-length forms of Gli3. Genes Dev 16(22):2843-8. [PubMed: 12435627]  [MGI Ref ID J:80205]

Ernest S; Christensen B; Gilfix BM; Mamer OA; Hosack A; Rodier M; Colmenares C; McGrath J; Bale A; Balling R; Sankoff D; Rosenblatt DS; Nadeau JH. 2002. Genetic and molecular control of folate-homocysteine metabolism in mutant mice. Mamm Genome 13(5):259-67. [PubMed: 12016514]  [MGI Ref ID J:76559]

McGannon P; Miyazaki Y; Gupta PC; Traboulsi EI; Colmenares C. 2006. Ocular abnormalities in mice lacking the Ski proto-oncogene. Invest Ophthalmol Vis Sci 47(10):4231-7. [PubMed: 17003410]  [MGI Ref ID J:116273]

Nomura T; Khan MM; Kaul SC; Dong HD; Wadhwa R; Colmenares C; Kohno I; Ishii S. 1999. Ski is a component of the histone deacetylase complex required for transcriptional repression by Mad and thyroid hormone receptor. Genes Dev 13(4):412-23. [PubMed: 10049357]  [MGI Ref ID J:53313]

Shinagawa T; Nomura T; Colmenares C; Ohira M; Nakagawara A; Ishii S. 2001. Increased susceptibility to tumorigenesis of ski-deficient heterozygous mice. Oncogene 20(56):8100-8. [PubMed: 11781823]  [MGI Ref ID J:73349]

Tesseur I; Wyss-Coray T. 2006. A role for TGF-beta signaling in neurodegeneration: evidence from genetically engineered models. Curr Alzheimer Res 3(5):505-13. [PubMed: 17168649]  [MGI Ref ID J:125213]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as heterozygotes. Homozygotes die shortly after birth.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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