Strain Name: |
B6.Cg H2g7-Tg(Ins2-CD80)3B7Flv/LwnJ |
|---|---|
Stock Number: |
005715 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
B6.Cg-H2g7-Tg(Ins2-CD80)3B7Flv/LwnJ (Changed: 28-SEP-06
) |
|
B6.Cg H2g7-Tg(Ins2-CD80)3B7Flv/FswJ (Changed: 07-MAR-06
) | |
| Strain Common Names | B6.H2g7-RIP-B7.1; B6.H2g7-RIP-CD80; B6.H2g7-RIPB7; |
| Genes & Alleles | CD80; H2; H2g7; Ins2; Tg(Ins2-CD80)3B7Flv; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Major Histocompatibility Congenic Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Background Strain B6.NOD-H2g7 Donor Strain (C57BL/6 X CBA)F2 Donating Investigator Li Wen, Yale School of Medicine H2 Haplotype g7 Generation N?F9+F2pN1 Appearance
black
Related Genotype: a/aStrain Description
Transgenic mice are characterized by pancreatic beta cells that express a rat insulin promoter (Ins2) regulated transgene encoding the human CD80 T cell co-stimulatory molecule. These mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. Better than 70% of the B6.H2g7 transgenic mice become diabetic by 30 weeks of age compared the control B6.H2g7 which does not develop insulitis or diabetes. Spleens of diabetic B6.H2g7 transgenic mice used in adoptive transfer experiments transfer diabetes to NOD.scid/RIP-B7.1 and irradiated non-diabetic B6.Cg H2g7-Tg(Ins2-CD80)3B7Flv/LwnJ mice, yet failed to transfer disease to NOD.scid, B6.scid, CB17.scid, or irradiated B6/RIP-B7.1.B6.Cg H2g7-Tg(Ins2-CD80)3B7Flv/LwnJ provides a tool for studying mechanisms of loss of tolerance in potentially diabetogenic CD8 T-cells.
Strain Development
A transgenic construct containing the human CD80 gene driven by the rat insulin promoter 1 (RIP) was injected fertilized eggs of a mating between C57BL/6 and CBA/Ca strains in the laboratory of Dr. Richard Flavell (Yale University). Founder animals were obtained and bred to C57BL/6 mice for more than 10 generations and subsequently mated to NOR/Lt or BALB/c for 10 generations and B6.NOD-H2g7 for 1 generation. In 2005, The Jackson Laboratory received this B6.Cg H2g7-Tg(Ins2-CD80)3B7Flv/LwnJ at N1F9.
Related Disease (OMIM) Terms |
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | H2g7 | ||
|---|---|---|---|
| Allele Name | g7 variant | ||
| Gene Symbol and Name | H2, histocompatibility-2, MHC | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | H-2; MHC-II; | ||
| General Note | The g7 variant has been observed in the following strains: DBR7, NON.NOD-H2g7 | ||
| Allele Symbol | Tg(Ins2-CD80)3B7Flv | ||
| Allele Name | transgene insertion 3B7, Richard Flavell | ||
| Common Name(s) | RIP-B7; RIP-B7-1; RIP-B7.1; RIP-CD80; | ||
| Mutation Made By | Richard Flavell, Yale University School of Medicine | ||
| Strain of Origin | (C57BL/6 x CBA/Ca)F2 | ||
| Expressed Gene | CD80, CD80 molecule, human | ||
| Promoter | Ins2, insulin 2, rat | ||
| General Note | Mice carrying this transgene that also are homozygous for Prkdcscid are characterized by pancreatic beta cells that express a rat insulin II promoter regulated transgene encoding the human CD80 T cell co-stimulatory molecule. | ||
| Molecular Note | The transgene contains a human CD80 antigen gene driven by the rat insulin II promoter (Ins2). [MGI Ref ID J:88250] | ||
Control Information
| Control | ||
|---|---|---|
| 003300 B6.NOD-(D17Mit21-D17Mit10)/LtJ | (approximate) | |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Ins2-CD80)3B7Flv
Related Strains
Strains carrying H2g7 allele
View Strains carrying H2g7 (9 strains)
005713 C.Cg-Tg(Ins2-CD80)3B7Flv/LwnJ 004346 NOD.Cg-Prkdcscid Tg(Ins2-CD80)3B7Flv/DvsJ 005714 NOR.Cg-Tg(Ins2-CD80)3B7Flv/LwnJ
Additional Web Information
Congenic Nomenclature
Genetic Quality Control Annual Report
Research Applications
This mouse can be used to support research in many areas including:
H2g7 relatedDiabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Research Tools
Diabetes and Obesity Research
Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
References
Selected Reference(s)
Additional ReferencesWong FS; Du W; Thomas IJ; Wen L. 2005. The influence of the major histocompatibility complex on development of autoimmune diabetes in RIP-B7.1 mice. Diabetes 54(7):2032-40. [PubMed: 15983204] [MGI Ref ID J:109830]
Price and Supply Information
| Strain Name: | B6.Cg H2g7-Tg(Ins2-CD80)3B7Flv/LwnJ |
| Stock Number: | 005715 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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- Use of MICE by companies or for-profit entities requires a license prior to shipping.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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