Strain Name:

B6(NOD) H2g7-Sostdc1shk/J

Stock Number:

005717

Availability:

Repository- Live

Description

Strain Information

Type Major Histocompatibility Congenic; Mutant Strain; Spontaneous Mutation;
Additional information on Genetically Engineered Mutant Mice.
Mating SystemHomozygote x Heterozygote         (Female x Male)
Mating SystemHeterozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
GenerationF4 (06-SEP-05)

Development
The sharkey (shk) mutation arose spontaneously in a C57BL/6J congenic strain homozygous for the NOD derived H2g7 and carrying a transgene. This transgene has been bred out of this strain, but the strain remains homozygous for H2g7. This mutant subline has been maintained by sibling intercrossing sharkey phenotypic homozygotes and unaffected heterozygotes. In 2007 this strain reached generation F8.

Control Information

  Control
   Heterozygote
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying   H2g7     (9 strains)

Strains carrying other alleles of H2
000471   A.SW-H2s H2-T18b/SnJ
002089   AK.B6-H2b Fv1b/J
002090   AK.B6-H2b/J
001094   AK.L-H2b/1CyTyJ
000469   B10.A-H2a H2-T18a/SgSnJ
000468   B10.A-H2h2/(2R)SgSnJ
001150   B10.A-H2h4/(4R)SgDvEgJ
000467   B10.A-H2i5 H2-T18a/(5R)SgSnJ
000465   B10.BR-H2k H2-T18a/SgSnJ
004804   B10.BR-H2k H2-T18a/SgSnJJrep
005308   B10.Cg-H2d Tg(TcraCl4,TcrbCl4)1Shrm/ShrmJ
005534   B10.Cg-H2d Tg(Ins2-HA)165Bri/ShrmJ
006102   B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J
006100   B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J
005895   B10.Cg-Thy1a H2d Tg(TcraCl1,TcrbCl1)1Shrm/J
002024   B10.D1-H2q/SgJ
000462   B10.D2-H2d/n2SnJ
001153   B10.D2-H2i7/(107R)EgJ
000460   B10.D2-Hc0 H2d H2-T18c/o2SnJ
000461   B10.D2-Hc0 H2d H2-T18c/oSnJ
000463   B10.D2-Hc1 H2d H2-T18c/nSnJ
003147   B10.D2-Hc1 H2d H2-T18c/nSnJ-Tg(DO11.10)10Dlo/J
001953   B10.S-H2s/SgMcdJ
002995   B6 x C.B10-H2b/LiMcdJ-Fbn2fp-2J/J
003584   B6.129S2-H2dlAb1-Ea/J
001148   B6.AK-H2k/FlaEgJ
001895   B6.AK-H2k/J
000360   B6.C-H2d Mdmg1BALB/cBy/aByJ
000359   B6.C-H2d/bByJ
000944   B6.SJL-H2b C3c/2CyJ
000966   B6.SJL-H2s C3c/1CyJ
000945   B6.SW/1CyJ
003374   B6;129S2-H2dlAb1-Ea/J
003240   B6;B10.A-H2a-Tg(H2KmPCC)2939Stoe/J
002844   BALB.5R-H2i5/LilJ
001041   BKS.B6-H2b/J
001892   BRVR.B10-H2b/J
001893   BRVR.D2-H2d/J
001952   C.B10-H2b/LilMcdJ
001951   C.C3-H2k/LilMcdJ
000438   C3.SW-H2b/SnJ
000437   D1.C-H2d H2-T18c/SnJ
000435   D1.LP-H2b H2-T18b?/SnJ
001383   LT.MA-Glo1b H2k/J
002591   NOD.B10Sn-H2b/J
004447   NOD.Cg-H2h4/DilTacUmmJ
002032   NOD.SW-H2q/J
003153   WLC.C-H2d.GR-Mtv2/MorJ
003154   WLC.C-H2d/MorJ
View Strains carrying other alleles of H2     (49 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Sostdc1shk/Sostdc1shk

        involves: C57BL/6J * NOD
  • craniofacial phenotype
  • abnormal hard palate (MGI Ref ID J:127012)
    • abnormal palatine shelf (MGI Ref ID J:127012)
      • asymetrical, disorganized formation
  • decreased skull height (MGI Ref ID J:127012)
    • females, but not males, have shorter skull height
  • decreased skull width (MGI Ref ID J:127012)
    • males have less skull morphological abnormalities than females, but do have smaller skull width than controls
  • short mandible (MGI Ref ID J:127012)
    • females, but not males, have a shorter jaw length than wildtype controls
  • supernumerary incisors (MGI Ref ID J:127012)
    • there is some variation in the rate of growth of these supernumerary incisors
    • an extra pair of upper and lower incisors erupt posterior to the normal incisors, and there are separate roots for these extra teeth
  • skeleton phenotype
  • abnormal palatine shelf (MGI Ref ID J:127012)
    • asymetrical, disorganized formation
  • decreased skull height (MGI Ref ID J:127012)
    • females, but not males, have shorter skull height
  • decreased skull width (MGI Ref ID J:127012)
    • males have less skull morphological abnormalities than females, but do have smaller skull width than controls
  • short mandible (MGI Ref ID J:127012)
    • females, but not males, have a shorter jaw length than wildtype controls
  • digestive/alimentary phenotype
  • abnormal hard palate (MGI Ref ID J:127012)
    • abnormal palatine shelf (MGI Ref ID J:127012)
      • asymetrical, disorganized formation

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Sostdc1shk/Sostdc1shk

        involves: C57BL/6J * CAST/EiJ * NOD
  • craniofacial phenotype
  • supernumerary incisors (MGI Ref ID J:127012)
    • F2 homozygotes from a cross to CAST/EiJ have upper, but not lower, supernumerary incisors
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

H2g7 related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Sostdc1shk related

Developmental Biology Research
Craniofacial and Palate Defects
Skeletal Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol H2g7
Allele Name g7 variant
Allele Type Not Applicable
Gene Symbol and Name H2, histocompatibility-2, MHC
Chromosome 17
Gene Common Name(s) H-2; MHC-II;
General Note The g7 variant has been observed in the following strains: DBR7, NON.NOD-H2g7
 
Allele Symbol Sostdc1shk
Allele Name sharkey
Allele Type Spontaneous
Strain of OriginB6.NOD-H2
Gene Symbol and Name Sostdc1, sclerostin domain containing 1
Chromosome 12
Gene Common Name(s) 0610006G05Rik; CDA019; DKFZp564D206; ECTODIN; RIKEN cDNA 0610006G05 gene; Sostl; USAG-1; USAG1; Wise; sclerostin-like;

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Giggey J; Curtain MM; Donahue LR. 2007. Sharkey, a new mutation in the Sostdc1 gene MGI Direct Data Submission :.  [MGI Ref ID J:127012]

Additional References

H2g7 related

Carrasco-Marin E; Shimizu J; Kanagawa O; Unanue ER. 1996. The class II MHC I-Ag7 molecules from non-obese diabetic mice are poor peptide binders. J Immunol 156(2):450-8. [PubMed: 8543793]  [MGI Ref ID J:30538]

Fossati G; Cooke A; Papafio RQ; Haskins K; Stockinger B. 1999. Triggering a second T cell receptor on diabetogenic T cells can prevent induction of diabetes. J Exp Med 190(4):577-83. [PubMed: 10449528]  [MGI Ref ID J:108724]

Gray D; Abramson J; Benoist C; Mathis D. 2007. Proliferative arrest and rapid turnover of thymic epithelial cells expressing Aire. J Exp Med 204(11):2521-8. [PubMed: 17908938]  [MGI Ref ID J:126040]

Jasinski JM; Yu L; Nakayama M; Li MM; Lipes MA; Eisenbarth GS; Liu E. 2006. Transgenic insulin (B:9-23) T-cell receptor mice develop autoimmune diabetes dependent upon RAG genotype, H-2g7 homozygosity, and insulin 2 gene knockout. Diabetes 55(7):1978-84. [PubMed: 16804066]  [MGI Ref ID J:111874]

Klein J; Figueroa F; David CS. 1983. H-2 haplotypes, genes and antigens: second listing. II. The H-2 complex. Immunogenetics 17(6):553-96. [PubMed: 6407984]  [MGI Ref ID J:7097]

Leiter EH. 1998. NOD Mice and Related Strains: Origins, Husbandry and Biology Introduction. In: NOD Mice and Related Strains: Research Applications in Diabetes, AIDS, Cancer, and Other Diseases. RG Landes, Austin.  [MGI Ref ID J:110093]

Levisetti MG; Lewis DM; Suri A; Unanue ER. 2008. Weak proinsulin peptide-major histocompatibility complexes are targeted in autoimmune diabetes in mice. Diabetes 57(7):1852-60. [PubMed: 18398138]  [MGI Ref ID J:138230]

Luhder F; Katz J; Benoist C; Mathis D. 1998. Major histocompatibility complex class II molecules can protect from diabetes by positively selecting T cells with additional specificities. J Exp Med 187(3):379-87. [PubMed: 9449718]  [MGI Ref ID J:108722]

Mahler M; Bristol IJ; Leiter EH; Workman AE; Birkenmeier EH; Elson CO; Sundberg JP. 1998. Differential susceptibility of inbred mouse strains to dextran sulfate sodium-induced colitis. Am J Physiol 274(3 Pt 1):G544-51. [PubMed: 9530156]  [MGI Ref ID J:46553]

Martin-Orozco N; Chen Z; Poirot L; Hyatt E; Chen A; Kanagawa O; Sharpe A; Mathis D; Benoist C. 2003. Paradoxical dampening of anti-islet self-reactivity but promotion of diabetes by OX40 ligand. J Immunol 171(12):6954-60. [PubMed: 14662903]  [MGI Ref ID J:86926]

Pearson T; Markees TG; Serreze DV; Pierce MA; Marron MP; Wicker LS; Peterson LB; Shultz LD; Mordes JP; Rossini AA; Greiner DL. 2003. Genetic disassociation of autoimmunity and resistance to costimulation blockade-induced transplantation tolerance in nonobese diabetic mice. J Immunol 171(1):185-95. [PubMed: 12816997]  [MGI Ref ID J:109845]

Podolin PL; Pressey A; DeLarato NH; Fischer PA; Peterson LB; Wicker LS. 1993. I-E+ nonobese diabetic mice develop insulitis and diabetes. J Exp Med 178(3):793-803. [PubMed: 8350054]  [MGI Ref ID J:14178]

Serreze DV; Gallichan WS; Snider DP; Croitoru K; Rosenthal KL; Leiter EH; Christianson GJ; Dudley ME; Roopenian DC. 1996. MHC class I-mediated antigen presentation and induction of CD8+ cytotoxic T-cell responses in autoimmune diabetes-prone NOD mice. Diabetes 45(7):902-8. [PubMed: 8666141]  [MGI Ref ID J:33688]

Turley SJ; Lee JW; Dutton-Swain N; Mathis D; Benoist C. 2005. Endocrine self and gut non-self intersect in the pancreatic lymph nodes. Proc Natl Acad Sci U S A 102(49):17729-33. [PubMed: 16317068]  [MGI Ref ID J:104385]

Wong FS; Du W; Thomas IJ; Wen L. 2005. The influence of the major histocompatibility complex on development of autoimmune diabetes in RIP-B7.1 mice. Diabetes 54(7):2032-40. [PubMed: 15983204]  [MGI Ref ID J:109830]

Yoshida T; Jiang F; Honjo T; Okazaki T. 2008. PD-1 deficiency reveals various tissue-specific autoimmunity by H-2b and dose-dependent requirement of H-2g7 for diabetes in NOD mice. Proc Natl Acad Sci U S A 105(9):3533-8. [PubMed: 18299579]  [MGI Ref ID J:132764]

Zhang C; Todorov I; Lin CL; Atkinson M; Kandeel F; Forman S; Zeng D. 2007. Elimination of insulitis and augmentation of islet beta cell regeneration via induction of chimerism in overtly diabetic NOD mice. Proc Natl Acad Sci U S A 104(7):2337-42. [PubMed: 17267595]  [MGI Ref ID J:119749]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           A1

Colony Maintenance

Breeding & HusbandryHomozygotes must have their teeth trimmed and attention must be paid to their ability to eat. The rate of supernumery tooth growth varies within the strain, with the most affected homozygotes requiring tooth trimming approximately once per week. In addition to standard food pellets, ground food should be supplied in the cage.
Mating SystemHomozygote x Heterozygote         (Female x Male)
Heterozygote x Homozygote         (Female x Male)

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*GenderGenotypes Provided
Individual Mouse Price $124.10Female or MaleHomozygous for H2g7, Heterozygous for Sostdc1shk
$201.10Female or MaleHomozygous for H2g7, Homozygous for Sostdc1shk
Pairs /Price*Pair Genotype
$248.20Homozygous for H2g7, Heterozygous for Sostdc1shk x Homozygous for H2g7, Heterozygous for Sostdc1shk
$325.20Homozygous for H2g7, Heterozygous for Sostdc1shk x Homozygous for H2g7, Homozygous for Sostdc1shk
$402.20Homozygous for H2g7, Homozygous for Sostdc1shk x Homozygous for H2g7, Homozygous for Sostdc1shk
*Price(s) in US dollars ($)

Additional Supply Details

Supply Notes

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*GenderGenotypes Provided
Individual Mouse Price $161.40Female or MaleHomozygous for H2g7, Heterozygous for Sostdc1shk
$261.50Female or MaleHomozygous for H2g7, Homozygous for Sostdc1shk
Pairs /Price*Pair Genotype
$322.70Homozygous for H2g7, Heterozygous for Sostdc1shk x Homozygous for H2g7, Heterozygous for Sostdc1shk
$422.80Homozygous for H2g7, Heterozygous for Sostdc1shk x Homozygous for H2g7, Homozygous for Sostdc1shk
$522.90Homozygous for H2g7, Homozygous for Sostdc1shk x Homozygous for H2g7, Homozygous for Sostdc1shk
*Price(s) in US dollars ($)

Additional Supply Details

Supply Notes

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes
  • Usually shipped between four and eight weeks of age.

Control Information

  Control
   Heterozygote
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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General Terms and Conditions


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General inquiries

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phone:207-288-6470
fax:207-288-6655

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