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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?+N1p Donating Investigator Raymond MacDonald, UTSW Medical Center Description
Hemizygous transgenic mice are viable, fertile, normal in size, and do not display any behavioral abnormalities. Expression of the bicistronic transgene is under the regulation of a tetracycline-responsive promoter element (TRE; tetO). When transgenic mice are bred to a second transgenic strain expressing tetracycline-transactivator (tTA) protein under the control of a tissue-specific promoter, the bitransgenic offspring express Ipf1 and lacZ in the appropriate target tissue. Further, Ipf1 and lacZ expression in bitransgenic mice can be suppressed by administration of the tetracycline analog, doxycycline. All cells expressing Ipf1 coexpress the reporter, and mRNA levels of the transgenic and endogenous Ipf1 fluctuate in concert during development.This mouse was designed originally to be mated to an pancreatic targeted mutant with tTAoff in place of the Ipf1 gene (see Stock No. 005701). The combined mutations allow normal pancreatic development and function until doxycycline treatment renders the double mutant mouse conditionally null for Ipf1 expression. In these double transgenic animals, pancreatic developmental can be arrested at stage during embyronic development or in adult mice. Such double mutant mice may be useful in studies of pancreatic endocrine/exocrine function and diabetes. Tg(tetO-Ipf1,lacZ)958.1Macd transgenic mice also may be bred with other tTA strains for conditional mutation analysis.
Development
A bicistronic transgenic vector was generated containing an Ipf1 minigene (both exons linked with a shortened intron and preceded by a truncated 5' untranslated region) and an internal ribosome entry site-linked beta-galactosidase (lacZ) all under transcriptional control of a tetracycline-responsive regulatory element (TRE). Transgenic mice were generated by pronuclear injection of the construct into fertilized [C57BL/6 x SJL] F1 hybrid embyros. Two-cell stage eggs were implanted into pseudopregnant foster mothers. Founder line 958.1 was obtained and was maintained by transgenic positive sibling intercross. At some point, transgenic mice were bred to B6;129 mice with a targeted mutation. The targeted mutation was selected against in subsequent breedings, and the transgenic line was again maintained by hemizygous intercrosses prior to cryopreservation.
Control Information
| Control | ||
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| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (186 strains)
005701 STOCK Pdx1tm1Macd/J 005699 STOCK Tg(tetO-Ipf1,EGFP)956.6Macd/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Tet Expression Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype relates to a compound genotype created using this strain.
Contact JAX® Services jaxservices@jax.org for customized breeding options.Pdx1tm1Macd/Pdx1tm1Macd Tg(tetO-Ipf1,lacZ)958.1Macd/0
involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL
- homeostasis/metabolism phenotype
- impaired glucose tolerance (MGI Ref ID J:103522)
- transgenic mice gradually attain an impaired glucose tolerance response to glucose challenge
- endocrine/exocrine gland phenotype
- abnormal pancreas development (MGI Ref ID J:103522)
- when dams are treated with doxycycline from conception through birth, only small epithelial remnants of the pancreas form; treatment at E7.5 or E8.5 results in the same phenotype
- doxycycline from E9.5 diminishes epithelial morphogenesis; treatement from E9.5 allows outgrowth of a linear epithelial tube with nascent invaginations representing initial primary branching
- treatment from E11.5 allows the formation of a larger crude duct-like structure with a few distal primary branches; treatement from E12.5 permits formation of extensive fine structure consisting of immature acini and associated small ductules
- with treatment from E12.5, pancreatic dorsal and ventral remnants composed of convoluted partially branched duct-like epithelium of columnar epithelium are present, and show a block at the stage of acinar cell formation
- examination of embryo ductal remnants from mice treated with dox from E11.5 show an absence of preacini; treatment from E12.5 results in a larger remnant with a smaller proportion of primitive duct; the epithelium is replaced by numerous eosinophilic clusters lacking ductal markers which are polarized, arranged around a central lumen and resemble immature acini
- absent pancreas (MGI Ref ID J:103522)
- ~1/5 pups are born without a pancreas; rescue of pancreas formation is observed in ~80% of transgenic mice
- digestive/alimentary phenotype
- abnormal pancreas development (MGI Ref ID J:103522)
- when dams are treated with doxycycline from conception through birth, only small epithelial remnants of the pancreas form; treatment at E7.5 or E8.5 results in the same phenotype
- doxycycline from E9.5 diminishes epithelial morphogenesis; treatement from E9.5 allows outgrowth of a linear epithelial tube with nascent invaginations representing initial primary branching
- treatment from E11.5 allows the formation of a larger crude duct-like structure with a few distal primary branches; treatement from E12.5 permits formation of extensive fine structure consisting of immature acini and associated small ductules
- with treatment from E12.5, pancreatic dorsal and ventral remnants composed of convoluted partially branched duct-like epithelium of columnar epithelium are present, and show a block at the stage of acinar cell formation
- examination of embryo ductal remnants from mice treated with dox from E11.5 show an absence of preacini; treatment from E12.5 results in a larger remnant with a smaller proportion of primitive duct; the epithelium is replaced by numerous eosinophilic clusters lacking ductal markers which are polarized, arranged around a central lumen and resemble immature acini
- absent pancreas (MGI Ref ID J:103522)
- ~1/5 pups are born without a pancreas; rescue of pancreas formation is observed in ~80% of transgenic mice
This mouse can be used to support research in many areas including:
lacZ relatedEndocrine Deficiency Research
Pancreas Defects
Metabolism Research
Enzyme Deficiency
exocrine pancreatic insufficiency
Research Tools
lacZ Expression
Diabetes and Obesity Research
lacZ
Genetics Research
Mutagenesis and Transgenesis: Tetop Tet System
Tissue/Cell Markers: multiple
Tissue/Cell Markers: pancreatic beta cells
Tet Expression Systems
tTA/rtTA Responsive Strains
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(tetO-Ipf1,lacZ)958.1Macd | ||
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| Allele Name | transgene insertion 958-1, Raymond J MacDonald | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | Pdx1-nlacZ; TgPdx1-lacZ; TgPdx1-nlacZ; TgPdx1; tetO-Pdx1; tetO-Pdx1-nlacZ; tetO-Pdx1/lacZ; tetO-Pdx1/nlacZ; | ||
| Mutation Made By | Raymond MacDonald, UTSW Medical Center | ||
| Strain of Origin | (C57BL/6 x SJL)F2 | ||
| Site of Expression | When crossed with a strain expressing tTA, bitransgenic progeny express the transgene in the target tissue. Expression of the transgene can be suppressed by the administration of tetracycline or its analog, doxycyline. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Expressed Gene | Pdx1, pancreatic and duodenal homeobox 1, mouse, laboratory | ||
| Promoter | tetO, tet operator, | ||
| Molecular Note | A transgenic vector was generated containing an Ipf1 minigene (both exons linked with a shortened intron). The Ipf1 gene was preceded by a truncated 5' UTR and an IRES-linked beta-galactosidase (lacZ) gene under transcriptional control of 7 direct repeats of the tetracycline operator sequence (tetO) fused to the human cytomegalovirus immediate-early promoter. [MGI Ref ID J:103522] | ||
| Gene Symbol and Name | Tg(tetO-Ipf1,lacZ)958.1Macd, transgene insertion 958-1, Raymond J MacDonald | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Pdx1-nlacZ; TgPdx1-lacZ; TgPdx1-nlacZ; TgPdx1; tetO-Pdx1; tetO-Pdx1-nlacZ; tetO-Pdx1/lacZ; tetO-Pdx1/nlacZ; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic LacZ Melt Curve Analysis, Melt Curve Analysis
Generic LacZ QPCR, QPCR
Generic LacZ, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Hale MA; Kagami H; Shi L; Holland AM; Elsasser HP; Hammer RE; MacDonald RJ. 2005. The homeodomain protein PDX1 is required at mid-pancreatic development for the formation of the exocrine pancreas. Dev Biol 286(1):225-37. [PubMed: 16126192] [MGI Ref ID J:103522]
Health & husbandry
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Health & Colony Maintenance Information
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are maintained as hemizygotes by transgenic positive sibling intercross.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 Cryopreserved Embryos $1600.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 Cryopreserved Embryos $2080.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Terms of Use
General Terms and Conditions
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
For additional Licensing and Use Restrictions view the link(s) below:
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