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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain NOD/ShiLt H2 Haplotype g7 Generation ?+N1p
Generation DefinitionsDonating Investigator Alexander Chervonsky, The University of Chicago Description
This strain expresses Cre recombinase in thymocytes and is active in T-cells during development.Development
The transgenic allele containing a Cre recombinase gene under the control of the Lck promoter from JR3802 - B6.Cg-Tg(Lck-cre)548Jxm/J was transferred to the NOD/Lt background through more than 10 generations of backcrossing. In 2005, the T1DR received hemizygous mice from this congenic NOD transgenic strain at generation N12.
Control Information
| Control | ||
|---|---|---|
| Noncarrier female | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying Tg(Lck-cre)548Jxm allele
003802 B6.Cg-Tg(Lck-cre)548Jxm/J View Strains carrying Tg(Lck-cre)548Jxm (1 strain)
019418 B6(Cg)-Tg(Lck-Cd1d1)1Aben/J 002817 B6.129S2-Lcktm1Mak/J 016101 B6.Cg-Il7rtm1Imx Tg(Lck-Il7r)1676Trma/J 013738 B6.Cg-Tg(LCKprBCL2L1)12Sjk/J 012837 B6.Cg-Tg(Lck-cre)3779Nik/J 006889 B6.Cg-Tg(Lck-cre)I540Jxm/J 003202 B6;SJL-Tg(CD8aCD8b)57Scr/J 002427 C3H/He-Tg(LCKprBCL2)36Sjk/J 008842 C57BL/6-Tg(Lck-VIPR2)1Ejg/J 003332 C57BL/6-Tg(LckNotch1)9Erob/J 002230 C57BL/6J-Tg(LckIl4)1315Dbl/J
Additional Web Information
Introduction to Cre-lox technology
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedDiabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains
NOD Transgenics
Research Tools
Cre-lox System
Cre Recombinase Expression
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Research Tools
Cre-lox System
Genetics Research
Mutagenesis and Transgenesis
Mutagenesis and Transgenesis: Cre-lox System
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Lck-cre)548Jxm | ||
|---|---|---|---|
| Allele Name | transgene insertion 548, Jamey Marth | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Lck-cre; Tg(Lck-cre); TgN(Lck-cre)548Jxm; | ||
| Mutation Made By | Jamey Marth, Burnham Inst at Univ Calif Santa Barbara | ||
| Strain of Origin | (C57BL/6 x CBA)F2 | ||
| Site of Expression | thymocytes | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Lck, lymphocyte protein tyrosine kinase, mouse, laboratory | ||
| Gene Symbol and Name | Tg(Lck-cre)548Jxm, transgene insertion 548, Jamey Marth | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Lck-cre; Tg(Lck-cre); TgN(Lck-cre)548Jxm; | ||
| Driver Note | Lck | ||
| General Note | Homozygous transgenic mice are viable and have no major defects. | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of the mouse lck promoter, active in the T cell lineage. [MGI Ref ID J:67674] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Garvin AM; Abraham KM; Forbush KA; Farr AG; Davison BL; Perlmutter RM. 1990. Disruption of thymocyte development and lymphomagenesis induced by SV40 T-antigen. Int Immunol 2(2):173-80. [PubMed: 1965144] [MGI Ref ID J:109934]
Additional References
Tg(Lck-cre)548Jxm relatedAzhar M; Yin M; Bommireddy R; Duffy JJ; Yang J; Pawlowski SA; Boivin GP; Engle SJ; Sanford LP; Grisham C; Singh RR; Babcock GF; Doetschman T. 2009. Generation of mice with a conditional allele for transforming growth factor beta 1 gene. Genesis 47(6):423-31. [PubMed: 19415629] [MGI Ref ID J:150024]
Bender TP; Kremer CS; Kraus M; Buch T; Rajewsky K. 2004. Critical functions for c-Myb at three checkpoints during thymocyte development. Nat Immunol 5(7):721-9. [PubMed: 15195090] [MGI Ref ID J:91122]
Bohgaki T; Mozo J; Salmena L; Matysiak-Zablocki E; Bohgaki M; Sanchez O; Strasser A; Hakem A; Hakem R. 2011. Caspase-8 inactivation in T cells increases necroptosis and suppresses autoimmunity in Bim-/- mice. J Cell Biol 195(2):277-91. [PubMed: 22006951] [MGI Ref ID J:177715]
Brewer JA; Khor B; Vogt SK; Muglia LM; Fujiwara H; Haegele KE; Sleckman BP; Muglia LJ. 2003. T-cell glucocorticoid receptor is required to suppress COX-2-mediated lethal immune activation. Nat Med 9(10):1318-22. [PubMed: 12949501] [MGI Ref ID J:97484]
Case AJ; McGill JL; Tygrett LT; Shirasawa T; Spitz DR; Waldschmidt TJ; Legge KL; Domann FE. 2011. Elevated mitochondrial superoxide disrupts normal T cell development, impairing adaptive immune responses to an influenza challenge. Free Radic Biol Med 50(3):448-58. [PubMed: 21130157] [MGI Ref ID J:168481]
Chang X; Liu F; Wang X; Lin A; Zhao H; Su B. 2011. The Kinases MEKK2 and MEKK3 Regulate Transforming Growth Factor-beta-Mediated Helper T Cell Differentiation. Immunity 34(2):201-12. [PubMed: 21333552] [MGI Ref ID J:168978]
Cheng Y; Zhang Z; Slape C; Aplan PD. 2007. Cre-loxP-mediated recombination between the SIL and SCL genes leads to a block in T-cell development at the CD4- CD8- to CD4+ CD8+ transition. Neoplasia 9(4):315-21. [PubMed: 17460775] [MGI Ref ID J:123860]
Cheung AM; Hande MP; Jalali F; Tsao MS; Skinnider B; Hirao A; McPherson JP; Karaskova J; Suzuki A; Wakeham A; You-Ten A; Elia A; Squire J; Bristow R; Hakem R; Mak TW. 2002. Loss of Brca2 and p53 Synergistically Promotes Genomic Instability and Deregulation of T-cell Apoptosis. Cancer Res 62(21):6194-204. [PubMed: 12414647] [MGI Ref ID J:79697]
Cully M; Elia A; Ong SH; Stambolic V; Pawson T; Tsao MS; Mak TW. 2004. grb2 heterozygosity rescues embryonic lethality but not tumorigenesis in pten+/- mice. Proc Natl Acad Sci U S A 101(43):15358-63. [PubMed: 15492213] [MGI Ref ID J:93530]
Delfino DV; Spinicelli S; Pozzesi N; Pierangeli S; Velardi E; Bruscoli S; Martelli MP; Pettirossi V; Falchi L; Kang TB; Riccardi C. 2011. Glucocorticoid-induced activation of caspase-8 protects the glucocorticoid-induced protein Gilz from proteasomal degradation and induces its binding to SUMO-1 in murine thymocytes. Cell Death Differ 18(1):183-90. [PubMed: 20671745] [MGI Ref ID J:186338]
Dougan SK; Rava P; Hussain MM; Blumberg RS. 2007. MTP regulated by an alternate promoter is essential for NKT cell development. J Exp Med 204(3):533-45. [PubMed: 17312007] [MGI Ref ID J:125365]
Dzhagalov I; Dunkle A; He YW. 2008. The anti-apoptotic Bcl-2 family member Mcl-1 promotes T lymphocyte survival at multiple stages. J Immunol 181(1):521-8. [PubMed: 18566418] [MGI Ref ID J:137400]
Eddahri F; Denanglaire S; Bureau F; Spolski R; Leonard WJ; Leo O; Andris F. 2009. Interleukin-6/STAT3 signaling regulates the ability of naive T cells to acquire B-cell help capacities. Blood 113(11):2426-33. [PubMed: 19020307] [MGI Ref ID J:146334]
Elftman MD; Hunzeker JT; Mellinger JC; Bonneau RH; Norbury CC; Truckenmiller ME. 2010. Stress-induced glucocorticoids at the earliest stages of herpes simplex virus-1 infection suppress subsequent antiviral immunity, implicating impaired dendritic cell function. J Immunol 184(4):1867-75. [PubMed: 20089700] [MGI Ref ID J:159473]
Fukuyama T; Kasper LH; Boussouar F; Jeevan T; van Deursen J; Brindle PK. 2009. Histone acetyltransferase CBP is vital to demarcate conventional and innate CD8+ T-cell development. Mol Cell Biol 29(14):3894-904. [PubMed: 19433445] [MGI Ref ID J:150146]
Gaudreau MC; Heyd F; Bastien R; Wilhelm B; Moroy T. 2012. Alternative splicing controlled by heterogeneous nuclear ribonucleoprotein L regulates development, proliferation, and migration of thymic pre-T cells. J Immunol 188(11):5377-88. [PubMed: 22523384] [MGI Ref ID J:188749]
Gebuhr TC; Kovalev GI; Bultman S; Godfrey V; Su L; Magnuson T. 2003. The role of Brg1, a catalytic subunit of mammalian chromatin-remodeling complexes, in T cell development. J Exp Med 198(12):1937-49. [PubMed: 14676303] [MGI Ref ID J:87219]
Genetic Resource Sciences at The Jackson Laboratory. 2012. Expression/Specificity Patterns of Cre Alleles, 2011 MGI Direct Data Submission :. [MGI Ref ID J:184578]
Guo F; Hildeman D; Tripathi P; Velu CS; Grimes HL; Zheng Y. 2010. Coordination of IL-7 receptor and T-cell receptor signaling by cell-division cycle 42 in T-cell homeostasis. Proc Natl Acad Sci U S A 107(43):18505-10. [PubMed: 20937872] [MGI Ref ID J:165512]
Hennet T; Hagen FK; Tabak LA; Marth JD. 1995. T-cell-specific deletion of a polypeptide N-acetylgalactosaminyl-transferase gene by site-directed recombination. Proc Natl Acad Sci U S A 92(26):12070-4. [PubMed: 8618846] [MGI Ref ID J:67674]
Herroeder S; Reichardt P; Sassmann A; Zimmermann B; Jaeneke D; Hoeckner J; Hollmann MW; Fischer KD; Vogt S; Grosse R; Hogg N; Gunzer M; Offermanns S; Wettschureck N. 2009. Guanine nucleotide-binding proteins of the G12 family shape immune functions by controlling CD4+ T cell adhesiveness and motility. Immunity 30(5):708-20. [PubMed: 19409815] [MGI Ref ID J:149560]
Hock H; Meade E; Medeiros S; Schindler JW; Valk PJ; Fujiwara Y; Orkin SH. 2004. Tel/Etv6 is an essential and selective regulator of adult hematopoietic stem cell survival. Genes Dev 18(19):2336-41. [PubMed: 15371326] [MGI Ref ID J:93052]
Jia W; He YW. 2011. Temporal regulation of intracellular organelle homeostasis in T lymphocytes by autophagy. J Immunol 186(9):5313-22. [PubMed: 21421856] [MGI Ref ID J:172749]
Jia W; Pua HH; Li QJ; He YW. 2011. Autophagy regulates endoplasmic reticulum homeostasis and calcium mobilization in T lymphocytes. J Immunol 186(3):1564-74. [PubMed: 21191072] [MGI Ref ID J:168905]
Jin J; Desai BN; Navarro B; Donovan A; Andrews NC; Clapham DE. 2008. Deletion of Trpm7 disrupts embryonic development and thymopoiesis without altering Mg2+ homeostasis. Science 322(5902):756-60. [PubMed: 18974357] [MGI Ref ID J:140630]
Kasper LH; Fukuyama T; Biesen MA; Boussouar F; Tong C; de Pauw A; Murray PJ; van Deursen JM; Brindle PK. 2006. Conditional knockout mice reveal distinct functions for the global transcriptional coactivators CBP and p300 in T-cell development. Mol Cell Biol 26(3):789-809. [PubMed: 16428436] [MGI Ref ID J:105505]
Khandanpour C; Phelan JD; Vassen L; Schutte J; Chen R; Horman SR; Gaudreau MC; Krongold J; Zhu J; Paul WE; Duhrsen U; Gottgens B; Grimes HL; Moroy T. 2013. Growth factor independence 1 antagonizes a p53-induced DNA damage response pathway in lymphoblastic leukemia. Cancer Cell 23(2):200-14. [PubMed: 23410974] [MGI Ref ID J:194309]
King CG; Kobayashi T; Cejas PJ; Kim T; Yoon K; Kim GK; Chiffoleau E; Hickman SP; Walsh PT; Turka LA; Choi Y. 2006. TRAF6 is a T cell-intrinsic negative regulator required for the maintenance of immune homeostasis. Nat Med 12(9):1088-92. [PubMed: 16921377] [MGI Ref ID J:131909]
Kirkland D; Benson A; Mirpuri J; Pifer R; Hou B; Defranco AL; Yarovinsky F. 2012. B Cell-Intrinsic MyD88 Signaling Prevents the Lethal Dissemination of Commensal Bacteria during Colonic Damage. Immunity 36(2):228-38. [PubMed: 22306056] [MGI Ref ID J:181331]
Kishimoto H; Ohteki T; Yajima N; Kawahara K; Natsui M; Kawarasaki S; Hamada K; Horie Y; Kubo Y; Arase S; Taniguchi M; Vanhaesebroeck B; Mak TW; Nakano T; Koyasu S; Sasaki T; Suzuki A. 2007. The Pten/PI3K pathway governs the homeostasis of Valpha14iNKT cells. Blood 109(8):3316-24. [PubMed: 17170126] [MGI Ref ID J:145339]
Konkel JE; Maruyama T; Carpenter AC; Xiong Y; Zamarron BF; Hall BE; Kulkarni AB; Zhang P; Bosselut R; Chen W. 2011. Control of the development of CD8alphaalpha(+) intestinal intraepithelial lymphocytes by TGF-beta. Nat Immunol 12(4):312-9. [PubMed: 21297643] [MGI Ref ID J:170346]
Krajewska M; You Z; Rong J; Kress C; Huang X; Yang J; Kyoda T; Leyva R; Banares S; Hu Y; Sze CH; Whalen MJ; Salmena L; Hakem R; Head BP; Reed JC; Krajewski S. 2011. Neuronal deletion of caspase 8 protects against brain injury in mouse models of controlled cortical impact and kainic acid-induced excitotoxicity. PLoS One 6(9):e24341. [PubMed: 21957448] [MGI Ref ID J:177668]
Lieu YK; Kumar A; Pajerowski AG; Rogers TJ; Reddy EP. 2004. Requirement of c-myb in T cell development and in mature T cell function. Proc Natl Acad Sci U S A 101(41):14853-8. [PubMed: 15466706] [MGI Ref ID J:93481]
Liu HH; Xie M; Schneider MD; Chen ZJ. 2006. Essential role of TAK1 in thymocyte development and activation. Proc Natl Acad Sci U S A 103(31):11677-82. [PubMed: 16857737] [MGI Ref ID J:111794]
Liu Y; Zhang P; Li J; Kulkarni AB; Perruche S; Chen W. 2008. A critical function for TGF-beta signaling in the development of natural CD4+CD25+Foxp3+ regulatory T cells. Nat Immunol 9(6):632-40. [PubMed: 18438410] [MGI Ref ID J:136291]
Luche H; Weber O; Nageswara Rao T; Blum C; Fehling HJ. 2007. Faithful activation of an extra-bright red fluorescent protein in 'knock-in' Cre-reporter mice ideally suited for lineage tracing studies. Eur J Immunol 37(1):43-53. [PubMed: 17171761] [MGI Ref ID J:117041]
Mak TW; Hakem A; McPherson JP; Shehabeldin A; Zablocki E; Migon E; Duncan GS; Bouchard D; Wakeham A; Cheung A; Karaskova J; Sarosi I; Squire J; Marth J; Hakem R. 2000. Brcal required for T cell lineage development but not TCR loci rearrangement. Nat Immunol 1(1):77-82. [PubMed: 10881179] [MGI Ref ID J:63282]
Mao X; Fujiwara Y; Chapdelaine A; Yang H; Orkin SH. 2001. Activation of EGFP expression by cre-mediated excision in a new ROSA26 reporter mouse strain Blood 97(1):324-6. [PubMed: 11133778] [MGI Ref ID J:66742]
Maresz K; Pryce G; Ponomarev ED; Marsicano G; Croxford JL; Shriver LP; Ledent C; Cheng X; Carrier EJ; Mann MK; Giovannoni G; Pertwee RG; Yamamura T; Buckley NE; Hillard CJ; Lutz B; Baker D; Dittel BN. 2007. Direct suppression of CNS autoimmune inflammation via the cannabinoid receptor CB1 on neurons and CB2 on autoreactive T cells. Nat Med 13(4):492-7. [PubMed: 17401376] [MGI Ref ID J:129619]
McPherson JP; Hande MP; Poonepalli A; Lemmers B; Zablocki E; Migon E; Shehabeldin A; Porras A; Karaskova J; Vukovic B; Squire J; Hakem R. 2006. A role for Brca1 in chromosome end maintenance. Hum Mol Genet 15(6):831-8. [PubMed: 16446310] [MGI Ref ID J:106759]
McPherson JP; Lemmers B; Hirao A; Hakem A; Abraham J; Migon E; Matysiak-Zablocki E; Tamblyn L; Sanchez-Sweatman O; Khokha R; Squire J; Hande MP; Mak TW; Hakem R. 2004. Collaboration of Brca1 and Chk2 in tumorigenesis. Genes Dev 18(10):1144-53. [PubMed: 15131084] [MGI Ref ID J:90512]
Mei Y; Zhang Y; Yamamoto K; Xie W; Mak TW; You H. 2009. FOXO3a-dependent regulation of Pink1 (Park6) mediates survival signaling in response to cytokine deprivation. Proc Natl Acad Sci U S A 106(13):5153-8. [PubMed: 19276113] [MGI Ref ID J:147146]
Mittelstadt PR; Monteiro JP; Ashwell JD. 2012. Thymocyte responsiveness to endogenous glucocorticoids is required for immunological fitness. J Clin Invest 122(7):2384-94. [PubMed: 22653054] [MGI Ref ID J:189951]
Miwa T; Zhou L; Kimura Y; Kim D; Bhandoola A; Song WC. 2009. Complement-dependent T-cell lymphopenia caused by thymocyte deletion of the membrane complement regulator Crry. Blood 113(12):2684-94. [PubMed: 19136662] [MGI Ref ID J:146538]
Nguyen TV; Ke Y; Zhang EE; Feng GS. 2006. Conditional deletion of Shp2 tyrosine phosphatase in thymocytes suppresses both pre-TCR and TCR signals. J Immunol 177(9):5990-6. [PubMed: 17056523] [MGI Ref ID J:140530]
O'Donnell N; Zachara NE; Hart GW; Marth JD. 2004. Ogt-dependent X-chromosome-linked protein glycosylation is a requisite modification in somatic cell function and embryo viability. Mol Cell Biol 24(4):1680-90. [PubMed: 14749383] [MGI Ref ID J:93112]
Pan L; Bradney C; Zheng B; Zhuang Y. 2004. Altered T-dependent antigen responses and development of autoimmune symptoms in mice lacking E2A in T lymphocytes. Immunology 111(2):147-54. [PubMed: 15027899] [MGI Ref ID J:88415]
Panattoni M; Sanvito F; Basso V; Doglioni C; Casorati G; Montini E; Bender JR; Mondino A; Pardi R. 2008. Targeted inactivation of the COP9 signalosome impairs multiple stages of T cell development. J Exp Med 205(2):465-77. [PubMed: 18268034] [MGI Ref ID J:132114]
Perruche S; Zhang P; Maruyama T; Bluestone JA; Saas P; Chen W. 2009. Lethal effect of CD3-specific antibody in mice deficient in TGF-beta1 by uncontrolled flu-like syndrome. J Immunol 183(2):953-61. [PubMed: 19561097] [MGI Ref ID J:151662]
Raetz M; Hwang SH; Wilhelm CL; Kirkland D; Benson A; Sturge CR; Mirpuri J; Vaishnava S; Hou B; Defranco AL; Gilpin CJ; Hooper LV; Yarovinsky F. 2013. Parasite-induced T(H)1 cells and intestinal dysbiosis cooperate in IFN-gamma-dependent elimination of Paneth cells. Nat Immunol 14(2):136-42. [PubMed: 23263554] [MGI Ref ID J:192614]
Rebholz B; Haase I; Eckelt B; Paxian S; Flaig MJ; Ghoreschi K; Nedospasov SA; Mailhammer R; Debey-Pascher S; Schultze JL; Weindl G; Forster I; Huss R; Stratis A; Ruzicka T; Rocken M; Pfeffer K; Schmid RM; Rupec RA. 2007. Crosstalk between Keratinocytes and Adaptive Immune Cells in an IkappaBalpha Protein-Mediated Inflammatory Disease of the Skin. Immunity 27(2):296-307. [PubMed: 17692539] [MGI Ref ID J:124343]
Ritprajak P; Hayakawa M; Sano Y; Otsu K; Park JM. 2012. Cell type-specific targeting dissociates the therapeutic from the adverse effects of protein kinase inhibition in allergic skin disease. Proc Natl Acad Sci U S A 109(23):9089-94. [PubMed: 22615377] [MGI Ref ID J:184745]
Saba I; Kosan C; Vassen L; Moroy T. 2011. IL-7R-dependent survival and differentiation of early T-lineage progenitors is regulated by the BTB/POZ domain transcription factor Miz-1. Blood 117(12):3370-81. [PubMed: 21258009] [MGI Ref ID J:170508]
Sagiv Y; Bai L; Wei DG; Agami R; Savage PB; Teyton L; Bendelac A. 2007. A distal effect of microsomal triglyceride transfer protein deficiency on the lysosomal recycling of CD1d. J Exp Med 204(4):921-8. [PubMed: 17403933] [MGI Ref ID J:125608]
Salmena L; Hakem R. 2005. Caspase-8 deficiency in T cells leads to a lethal lymphoinfiltrative immune disorder. J Exp Med 202(6):727-32. [PubMed: 16157684] [MGI Ref ID J:107455]
Salmena L; Lemmers B; Hakem A; Matysiak-Zablocki E; Murakami K; Au PY; Berry DM; Tamblyn L; Shehabeldin A; Migon E; Wakeham A; Bouchard D; Yeh WC; McGlade JC; Ohashi PS; Hakem R. 2003. Essential role for caspase 8 in T-cell homeostasis and T-cell-mediated immunity. Genes Dev 17(7):883-95. [PubMed: 12654726] [MGI Ref ID J:82759]
Schmedt C; Tarakhovsky A. 2001. Autonomous maturation of alpha/beta T lineage cells in the absence of COOH-terminal Src kinase (Csk). J Exp Med 193(7):815-26. [PubMed: 11283154] [MGI Ref ID J:120548]
Shui JW; Hu MC; Tan TH. 2007. Conditional knockout mice reveal an essential role of protein phosphatase 4 in thymocyte development and pre-T-cell receptor signaling. Mol Cell Biol 27(1):79-91. [PubMed: 17060460] [MGI Ref ID J:117684]
Stephensen CB; Borowsky AD; Lloyd KC. 2007. Disruption of Rxra gene in thymocytes and T lymphocytes modestly alters lymphocyte frequencies, proliferation, survival and T helper type 1/type 2 balance. Immunology 121(4):484-98. [PubMed: 17433077] [MGI Ref ID J:125537]
Stranges PB; Watson J; Cooper CJ; Choisy-Rossi CM; Stonebraker AC; Beighton RA; Hartig H; Sundberg JP; Servick S; Kaufmann G; Fink PJ; Chervonsky AV. 2007. Elimination of antigen-presenting cells and autoreactive T cells by Fas contributes to prevention of autoimmunity. Immunity 26(5):629-41. [PubMed: 17509906] [MGI Ref ID J:123556]
Suliman S; Tan J; Xu K; Kousis PC; Kowalski PE; Chang G; Egan SE; Guidos C. 2011. Notch3 is dispensable for thymocyte beta-selection and Notch1-induced T cell leukemogenesis. PLoS One 6(9):e24937. [PubMed: 21931869] [MGI Ref ID J:177688]
Suzuki A; Nakano T; Mak TW; Sasaki T. 2008. Portrait of PTEN: messages from mutant mice. Cancer Sci 99(2):209-13. [PubMed: 18201277] [MGI Ref ID J:142484]
Suzuki A; Yamaguchi MT; Ohteki T; Sasaki T; Kaisho T; Kimura Y; Yoshida R; Wakeham A; Higuchi T; Fukumoto M; Tsubata T; Ohashi PS; Koyasu S; Penninger JM; Nakano T; Mak TW. 2001. T cell-specific loss of pten leads to defects in central and peripheral tolerance. Immunity 14(5):523-34. [PubMed: 11371355] [MGI Ref ID J:69471]
Ueda T; Sasaki M; Elia AJ; Chio II; Hamada K; Fukunaga R; Mak TW. 2010. Combined deficiency for MAP kinase-interacting kinase 1 and 2 (Mnk1 and Mnk2) delays tumor development. Proc Natl Acad Sci U S A 107(32):13984-90. [PubMed: 20679220] [MGI Ref ID J:163707]
Ventura A; Meissner A; Dillon CP; McManus M; Sharp PA; Van Parijs L; Jaenisch R; Jacks T. 2004. Cre-lox-regulated conditional RNA interference from transgenes. Proc Natl Acad Sci U S A 101(28):10380-5. [PubMed: 15240889] [MGI Ref ID J:129426]
Wang HC; Peng V; Zhao Y; Sun XH. 2012. Enhanced Notch activation is advantageous but not essential for T cell lymphomagenesis in Id1 transgenic mice. PLoS One 7(2):e32944. [PubMed: 22393458] [MGI Ref ID J:185287]
Wang X; Chang X; Facchinetti V; Zhuang Y; Su B. 2009. MEKK3 is essential for lymphopenia-induced T cell proliferation and survival. J Immunol 182(6):3597-608. [PubMed: 19265138] [MGI Ref ID J:145923]
Wang X; Zhang F; Chen F; Liu D; Zheng Y; Zhang Y; Dong C; Su B. 2011. MEKK3 regulates IFN-gamma production in T cells through the Rac1/2-dependent MAPK cascades. J Immunol 186(10):5791-800. [PubMed: 21471448] [MGI Ref ID J:173095]
Weischenfeldt J; Damgaard I; Bryder D; Theilgaard-Monch K; Thoren LA; Nielsen FC; Jacobsen SE; Nerlov C; Porse BT. 2008. NMD is essential for hematopoietic stem and progenitor cells and for eliminating by-products of programmed DNA rearrangements. Genes Dev 22(10):1381-96. [PubMed: 18483223] [MGI Ref ID J:134765]
Wong GW; Knowles GC; Mak TW; Ferrando AA; Zuniga-Pflucker JC. 2012. HES1 opposes a PTEN-dependent check on survival, differentiation, and proliferation of TCRbeta-selected mouse thymocytes. Blood 120(7):1439-48. [PubMed: 22649105] [MGI Ref ID J:189112]
Woolfenden S; Zhu H; Charest A. 2009. A Cre/LoxP conditional luciferase reporter transgenic mouse for bioluminescence monitoring of tumorigenesis. Genesis 47(10):659-666. [PubMed: 19603508] [MGI Ref ID J:153565]
Xue L; Chiang L; He B; Zhao YY; Winoto A. 2010. FoxM1, a forkhead transcription factor is a master cell cycle regulator for mouse mature T cells but not double positive thymocytes. PLoS One 5(2):e9229. [PubMed: 20169079] [MGI Ref ID J:157994]
Xue L; Nolla H; Suzuki A; Mak TW; Winoto A. 2008. Normal development is an integral part of tumorigenesis in T cell-specific PTEN-deficient mice. Proc Natl Acad Sci U S A 105(6):2022-7. [PubMed: 18250301] [MGI Ref ID J:131807]
You H; Pellegrini M; Tsuchihara K; Yamamoto K; Hacker G; Erlacher M; Villunger A; Mak TW. 2006. FOXO3a-dependent regulation of Puma in response to cytokine/growth factor withdrawal. J Exp Med 203(7):1657-63. [PubMed: 16801400] [MGI Ref ID J:124403]
Young JA; Becker AM; Medeiros JJ; Shapiro VS; Wang A; Farrar JD; Quill TA; van Huijsduijnen RH; van Oers NS. 2008. The protein tyrosine phosphatase PTPN4/PTP-MEG1, an enzyme capable of dephosphorylating the TCR ITAMs and regulating NF-kappaB, is dispensable for T cell development and/or T cell effector functions. Mol Immunol 45(14):3756-66. [PubMed: 18614237] [MGI Ref ID J:138871]
Zhang N; He YW. 2005. An essential role for c-FLIP in the efficient development of mature T lymphocytes. J Exp Med 202(3):395-404. [PubMed: 16043517] [MGI Ref ID J:100558]
Zhang N; He YW. 2005. The antiapoptotic protein Bcl-xL is dispensable for the development of effector and memory T lymphocytes. J Immunol 174(11):6967-73. [PubMed: 15905539] [MGI Ref ID J:99012]
Zhang N; Hopkins K; He YW. 2008. The long isoform of cellular FLIP is essential for T lymphocyte proliferation through an NF-kappaB-independent pathway. J Immunol 180(8):5506-11. [PubMed: 18390734] [MGI Ref ID J:134252]
Zheng L; Njauw CN; Martins-Green M. 2007. A hCXCR1 transgenic mouse model containing a conditional color-switching system for imaging of hCXCL8/IL-8 functions in vivo. J Leukoc Biol 82(5):1247-56. [PubMed: 17704296] [MGI Ref ID J:127327]
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Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2250.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2925.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- This strain is included in the Type 1 Diabetes Repository collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier female | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.