Strain Name: |
NOD.Cg-Tg(Lck-cre)548Jxm/AchJ |
|---|---|
Stock Number: |
005732 |
Availability: | Repository-Cryopreserved |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | Lck; cre; Tg(Lck-cre)548Jxm; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Background Strain NOD/ShiLt Donating Investigator Alexander Chervonsky, The University of Chicago H2 Haplotype g7 Strain Description
This strain expresses Cre recombinase in thymocytes and is active in T-cells during development.Strain Development
The transgenic allele containing a Cre recombinase gene under the control of the Lck promoter from JR3802 - B6.Cg-Tg(Lck-cre)548Jxm/J was transferred to the NOD/Lt background through more than 10 generations of backcrossing. In 2005, the T1DR received hemizygous mice from this congenic NOD transgenic strain at generation N12.
Gene & Allele Details
| Allele Symbol | Tg(Lck-cre)548Jxm | ||
|---|---|---|---|
| Allele Name | transgene insertion 548, Jamey Marth | ||
| Common Name(s) | Lck-cre; Tg(Lck-cre); TgN(Lck-cre)548Jxm; | ||
| Mutation Made By | Jamey Marth, University of California at San Diego | ||
| Strain of Origin | (C57BL/6 x CBA)F2 | ||
| Site of Expression | thymocytes | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Lck, lymphocyte protein tyrosine kinase, mouse, laboratory | ||
| General Note | Homozygous transgenic mice are viable and have no major defects. | ||
| Molecular Note | This transgene expresses Cre recombinase under the control of the mouse lck promoter, active in the T cell lineage. [MGI Ref ID J:67674] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier female | ||
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Generic Cre
Related Strains
Strains carrying Tg(Lck-cre)548Jxm allele
003802 B6.Cg-Tg(Lck-cre)548Jxm/J View Strains carrying Tg(Lck-cre)548Jxm (1 strain)
002817 B6.129S2-Lcktm1Mak/J 002427 C3H/He-Tg(LCKprBCL2)36Sjk/J 003332 C57BL/6-Tg(LckNotch1)9Erob/J 002230 C57BL/6J-Tg(LckIl4)1315Dbl/J
Additional Web Information
Congenic Nomenclature
Cre-lox or FLP-FRT Systems
Research Applications
This mouse can be used to support research in many areas including:
cre relatedDiabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)
Research Tools
Cre-lox System (Cre Recombinase Expression)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
References
Selected Reference(s)
Additional ReferencesGarvin AM; Abraham KM; Forbush KA; Farr AG; Davison BL; Perlmutter RM. 1990. Disruption of thymocyte development and lymphomagenesis induced by SV40 T-antigen. Int Immunol 2(2):173-80. [PubMed: 1965144] [MGI Ref ID J:109934]
Price and Supply Information
| Strain Name: | NOD.Cg-Tg(Lck-cre)548Jxm/AchJ |
| Stock Number: | 005732 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
International
| Price(s) in US dollars ($) | |||||
|---|---|---|---|---|---|
| Cryorecovery Fee | $2470.00 | ||||
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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