Strain Name: |
NOD/ShiLt-Tg(Ins2-Fas*I246N)1Ach/AchJ |
|---|---|
Stock Number: |
005733 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
NOD/Lt-Tg(Ins2-Fas*I246N)1Ach/AchJ (Changed: 23-FEB-07
) |
|
NOD/Lt-Tg(Ins2-Fas)1Ach/AchJ (Changed: 05-OCT-06
) | |
|
NOD/Lt-Tg(Ins2-Fascg)1Ach/AchJ (Changed: 04-OCT-06
) | |
| Strain Common Names | NOD-Tg(Ins2-Tnfrsf6lpr-cg)/Ach; NOD.RIP-Fascg; NOD.Fascg; NOD.RIP-Fas-lprcg; NOD.RIP-Fascg Tg-Hi; |
| Genes & Alleles | Fas; Ins2; Tg(Ins2-Fas*I246N)1Ach; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Coisogenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Alexander Chervonsky, The University of Chicago Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/TyrcStrain Description
Diabetes onset is significantly delayed in Rip-Fascg transgenic mice (45% diabetic by 30 weeks of age) compared to wildtype cohorts (90% diabetic by 30 weeks of age). Adoptive transfer studies using splenocytes from diabetic NOD/Lt mice into Rip-Fascg transgenic mice indicate the transgenic mice become diabetic later than wildtype controls. This transgenic strain is useful in studies looking at the effects of Fas on pancreatic islets.Strain Development
Fas cDNA with a single nucleotide change leading to a I246N change in protein sequence, which codes for Fascg was subcloned into the rat insulin 2 promoter and injected into NOD/Lt oocytes. In 2006, the T1DR received mice at generation N?F8.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(Ins2-Fas*I246N)1Ach | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Alexander V Chervonsky | ||
| Common Name(s) |
RIP-Fas-lprcg;
RIP-Fascg;
RIP-Fas | ||
| Strain of Origin | NOD/ShiLt | ||
| Expressed Gene | Fas, Fas (TNF receptor superfamily, member 6), rat | ||
| Promoter | Ins2, insulin 2, rat | ||
| Molecular Note | Fas cDNA with a single nucleotide change leading to a I246N change in protein sequence, which codes for Fascg was subcloned into the rat insulin 2 promoter and injected into NOD/Lt oocytes to produce transgenic mice. [MGI Ref ID J:81416] | ||
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Ins2-Fas*I246N)1Ach
Related Strains
Strains carrying other alleles of Ins2
View Strains carrying other alleles of Ins2 (42 strains)
Additional Web Information
Genetic Quality Control Annual Report
Research Applications
This mouse can be used to support research in many areas including:
Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)
References
Selected Reference(s)
Savinov AY; Tcherepanov A; Green EA; Flavell RA; Chervonsky AV. 2003. Contribution of Fas to diabetes development. Proc Natl Acad Sci U S A 100(2):628-32. [PubMed: 12525697] [MGI Ref ID J:81416]
Price and Supply Information
| Strain Name: | NOD/ShiLt-Tg(Ins2-Fas*I246N)1Ach/AchJ |
| Stock Number: | 005733 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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