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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
Description
Strain Information
Type Coisogenic; Mutant Strain; Transgenic; Species laboratory mouse Generation ?+N1p (19-FEB-06) Donating Investigator Alexander Chervonsky, The University of Chicago Description
Tg(Ins2-rtTA)2Ach encodes the reverse tetracycline regulatable transactivator (rtTA) regulated by the rat insulin promoter (Ins2, commonly designated RIP), and is expressed in the pancreatic beta cells. Hemizygous transgenic mice are viable, fertile, normal in size, display normal NOD diabetes onset, but do not display any other gross physical or behavioral abnormalities. When NOD/Lt-Tg(Ins2-rtTA)2Ach/AchJ mice are mated to a second transgenic strain carrying a target gene regulated by the tetO responsive elements, expression of the target gene is turned on by the tetracycline analog, doxycycline (dox). Dox can be administered orally in the food or water. This strain provides a Tet-On tool that permits the inducible expression of genes in the pancreatic beta cells during various stages of diabetes development (As reported by donating investigator, data unpublished).This transgenic strain is likely to have characteristics similar to Stock No. 004602. Note that the genetic background this strain is comprised entirely of NOD/ShtLt whereas Stock No. 004602 background is largely NOD/ShiLt but may have contributions from the C57BL/6J and SJL inbred strains.
Development
Alexander Chervonsky, et al., injected the Ins2-rtTA transgenic construct directly into NOD/ShiLt oocytes. The resulting transgenic line has been maintained by continual matings to NOD/ShiLt. In 2005, The T1DR received this NOD/ShiLt transgenic which is likely to have characteristics similar to Stock No. 004602. Note that the genetic background of this strain is comprised entirely of NOD/ShiLt whereas Stock No. 004602 background is largely NOD/ShiLt but may have contributions from the C57BL/6J inbred strain.
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Ins2
View Strains carrying other alleles of Ins2 (42 strains)
005670 B6.Cg-Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 006235 B6.Cg-Tg(SFTPC-rtTA)5Jaw/J 006232 B6.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 007678 B6;SJL-Tg(KRT14-rtTA)208Jek/J 006245 C.Cg-Tg(SFTPC-rtTA)5Jaw/J 006242 C.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 008099 FVB-Tg(KRT14-rtTA)F42Efu/J 004127 FVB-Tg(Nes-rtTA)306Rvs/J 006225 FVB.Cg-Tg(SFTPC-rtTA)5Jaw/J 006222 FVB.Cg-Tg(Scgb1a1-rtTA)1Jaw/J 008202 FVB/N-Tg(NPHS2-rtTA2*M2)1Jbk/J 006875 FVB/N-Tg(Tagln-rtTA)E1Jwst/J 004602 NOD.Cg-Tg(Ins2-rtTA)2Doi/DoiJ 005572 STOCK Gt(ROSA)26Sortm1(rtTA,EGFP)Nagy/J 008250 STOCK Tg(Ins2-rtTA)2Efr/J 005493 STOCK Tg(Tek-rtTA,TRE-lacZ)1425Tpr/J 003273 STOCK Tg(rtTAhCMV)4Bjd/J
Additional Web Information
Genetic Quality Control Annual Report
Tet Expression Systems
Phenotype
Phenotype Information
Mammalian Phenotype Terms assigned by genotype
Tg(Ins2-rtTA)1Ach/0
NOD/ShiLt-Tg(Ins2-rtTA)1Ach
- normal phenotype
- no abnormal phenotype detected (MGI Ref ID J:127424)
- hemizygous mice are viable, fertile, and display no obvious physical or behavioral abnormalities; mice display normal diabetes onset relative to control NOD littermates
Research Applications
This mouse can be used to support research in many areas including:
Research Tools
Tet Expression Systems (tTA/rtTA Expressing Strains)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Ins2-rtTA)1Ach | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Alexander Chervonsky | ||
| Common Name(s) | RIP-rtTA; | ||
| Strain of Origin | NOD/ShiLt | ||
| Site of Expression | Expresses tTA in pancreas. | ||
| Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli | ||
| The tetracycline repressor gene (Tetr), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). One mutant with a four amino acid residue change (rTetR) exhibited dependence on tetracycline for induction of the targeted gene and was used in the rtTA construct (Gossen et al, 1995). rTetr was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). | |||
| Promoter | Ins2, insulin 2, rat | ||
| Molecular Note | The transgene encodes the reverse tetracycline regulatable transactivator (rtTA) regulated by the rat insulin promoter (Ins2) and is expressed in the pancreatic beta cells. [MGI Ref ID J:127424] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tTA), MCA, vers. 4
Tg(tTA), QPCR, vers. 3
Tg(tTA), STD PCR, vers. 2
Helpful Links
Optimizing PCR Protocols
References
References
Additional References
Tg(Ins2-rtTA)1Ach related
Chervonsky A. 2007. Mice expressing a reverse tetracycline regulatable transactivator transgene under control of the rat insulin 2 promoter MGI Direct Data Submission :. [MGI Ref ID J:127424]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00 Cryopreserved Embryos Fee $1600.00
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00 Cryopreserved Embryos Fee $2080.00
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| 001976 NOD/ShiLtJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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