| |||||||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Stock; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 10-MAR-09 Species laboratory mouse Generation N6+F6 (18-AUG-09) Donating Investigator Ian McLennan, University of Otago Description
Mice homozygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Green Fluorescent Protein (EGFP) and a truncated dominant-negative mouse transforming growth factor, beta receptor II (Tgfbr2) under the control of a bidirectional tetracycline transactivator responsive promoter (TRE; tetO). No fluorescence is observed in the tissues of this mutant. Primary cultures derived from ear biopsies exhibit green fluorescence when transfected with a tetracycline transactivator (tTA; "Tet-off") vector. When these transgenic mice are bred with other transgenic mice expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue-specific EGFP and dominant-negative Tgfbr2 transgene expression can be regulated with the tetracycline analog, doxycycline. This mutant mouse strain may be useful in studies of TGF-beta signaling.Development
A transgenic construct containing an Enhanced Green Fluorescent Protein gene and a truncated mouse transforming growth factor, beta receptor II gene under the control of the a bidirectional tetracycline-responsive promoter (TRE; tetO) was microinjected into fertilized FVB/N oocytes. Founder line 8 (H) was subsequently established. The mice were then backcrossed to the C57BL/6 background for 5 generations.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (225 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cell Biology Research
Fluorescent Proteins
Genetics Research
Tissue/Cell Markers
Immunology and Inflammation Research
Tet Expression Systems
tTA/rtTA Responsive Strains
Research Tools
Fluorescent Proteins
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(tetO-EGFP,-Tgfbr2)8Mcle | ||
|---|---|---|---|
| Allele Name | transgene insertion 8, Ian S McLennan | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | PTR; PTR (line 8); PTRh; | ||
| Mutation Made By | Ian McLennan, University of Otago | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | Primary cultures derived from ear biopsies exhibit green fluorescence when transfected with a tetracycline transactivator (Tet-off) vector. When bred with another transgenic mouse expressing rtTA or tTA to create a bitransgenic animal, tissue specific EGFP & dominant-negative mouse mouse transforming growth factor, beta receptor II transgene expression can be regulated with the tetracy | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | Tgfbr2, transforming growth factor, beta receptor II, mouse, laboratory | ||
| Promoter | tetO, tet operator, | ||
| General Note | Of 17 transgenic mice derived from microinjected oocytes, 6 yielded cell cultures that strongly expressed EGFP upon transfection with a tetracycline transactivator- (tTA-; "tet-off") expressing plasmid. Offspring of two transgenic founder lines, designated by the authors PTRe and PTRh, by tTA transgenic mice were subjected to further expression analysis. | ||
| Molecular Note | The transgene comprises a bidirectional tetracycline transactivator responsive promoter (tetO) between an enhanced green fluorescent protein gene with a polyadenylation (poly[A]) signal derived from simian virus 40 (SV40) and a truncated transforming growth factor, beta receptor II cDNA with a c-Myc epitope tag and a beta-globin gene-derived poly[A] signal. The encoded TGFBR2 protein, which lacks the intracellular kinase domain, functions as a dominant negative receptor, disrupting TGFbeta signaling. Tissues of transgenic mice do not fluoresce. Coordinate expression of EGFP and dominant-negative TGFBR2 can be regulated in a tissue-specific manner in bitransgenic mice with this and a tetracycline transactivator (tTA; "tet-off") or reverse transactivator (rtTA; "tet-on") transgene by administration/withdrawal of the tetracycline analog doxycycline. [MGI Ref ID J:109835] | ||
| Gene Symbol and Name | Tg(tetO-EGFP,-Tgfbr2)8Mcle, transgene insertion 8, Ian S McLennan | ||
| Chromosome | UN | ||
| Gene Common Name(s) | PTR; PTR (line 8); PTRh; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Fluorescent Proteins (Generic GFP), Melt Curve Analysis
Fluorescent Proteins (Generic GFP), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Frugier T; Koishi K; Matthaei KI; McLennan IS. 2005. Transgenic mice carrying a tetracycline-inducible, truncated transforming growth factor beta receptor (TbetaRII). Genesis 42(1):1-5. [PubMed: 15828000] [MGI Ref ID J:109835]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX11
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, these mice are bred as homozygotes. Mating System Homozygote x Homozygote (Female x Male) 10-MAR-09 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $209.90 Female or Male Homozygous for Tg(tetO-EGFP,-Tgfbr2)8Mcle
Pairs /Price (US dollars $) Pair Genotype $419.80 Homozygous for Tg(tetO-EGFP,-Tgfbr2)8Mcle x Homozygous for Tg(tetO-EGFP,-Tgfbr2)8Mcle
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $272.90 Female or Male Homozygous for Tg(tetO-EGFP,-Tgfbr2)8Mcle
Pairs /Price (US dollars $) Pair Genotype $545.80 Homozygous for Tg(tetO-EGFP,-Tgfbr2)8Mcle x Homozygous for Tg(tetO-EGFP,-Tgfbr2)8Mcle
Additional Supply Details
|
|
|
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.