Strain Name: |
B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J |
|---|---|
Stock Number: |
005738 |
Availability: | Repository- Live |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | GFP; Tgfbr2; Tg(tetO-EGFP,-Tgfbr2)8Mcle; tetO; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Stock Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Ian McLennan, University of Otago Generation N6+F1 (03-DEC-07) Strain Description
Mice homozygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express Enhanced Green Fluorescent Protein (EGFP) and a truncated dominant-negative mouse transforming growth factor, beta receptor II (Tgfbr2) under the control of a bidirectional tetracycline transactivator responsive promoter (TRE; tetO). No fluorescence is observed in the tissues of this mutant. Primary cultures derived from ear biopsies exhibit green fluorescence when transfected with a tetracycline transactivator (tTA; "Tet-off") vector. When these transgenic mice are bred with other transgenic mice expressing reverse tetracycline-controlled transactivator protein (rtTA) or tetracycline-controlled transactivator protein (tTA), tissue-specific EGFP and dominant-negative Tgfbr2 transgene expression can be regulated with the tetracycline analog, doxycycline. This mutant mouse strain may be useful in studies of TGF-beta signaling.Strain Development
A transgenic construct containing an Enhanced Green Fluorescent Protein gene and a truncated mouse transforming growth factor, beta receptor II gene under the control of the a bidirectional tetracycline-responsive promoter (TRE; tetO) was microinjected into fertilized FVB/N oocytes. Founder line 8 (H) was subsequently established. The mice were then backcrossed to the C57BL/6 background for 5 generations.
Gene & Allele Details
| Allele Symbol | Tg(tetO-EGFP,-Tgfbr2)8Mcle | ||
|---|---|---|---|
| Allele Name | transgene insertion 8, Ian S McLennan | ||
| Common Name(s) | PTR; PTR (line 8); PTRh; | ||
| Mutation Made By | Ian McLennan, University of Otago | ||
| Strain of Origin | FVB/N | ||
| Site of Expression | Primary cultures derived from ear biopsies exhibit green fluorescence when transfected with a tetracycline transactivator (Tet-off) vector. When bred with another transgenic mouse expressing rtTA or tTA to create a bitransgenic animal, tissue specific EGFP & dominant-negative mouse mouse transforming growth factor, beta receptor II transgene expression can be regulated with the tetracy | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. The original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Expressed Gene | Tgfbr2, transforming growth factor, beta receptor II, mouse, laboratory | ||
| Promoter | tetO, tet operator, | ||
| General Note | Of 17 transgenic mice derived from microinjected oocytes, 6 yielded cell cultures that strongly expressed EGFP upon transfection with a tetracycline transactivator- (tTA-; "tet-off") expressing plasmid. Offspring of two transgenic founder lines, designated by the authors PTRe and PTRh, by tTA transgenic mice were subjected to further expression analysis. | ||
| Molecular Note | The transgene comprises a bidirectional tetracycline transactivator responsive promoter (tetO) between an enhanced green fluorescent protein gene with a polyadenylation (poly[A]) signal derived from simian virus 40 (SV40) and a truncated transforming growth factor, beta receptor II cDNA with a c-Myc epitope tag and a beta-globin gene-derived poly[A] signal. The encoded TGFBR2 protein, which lacks the intracellular kinase domain, functions as a dominant negative receptor, disrupting TGFbeta signaling. Tissues of transgenic mice do not fluoresce. Coordinate expression of EGFP and dominant-negative TGFBR2 can be regulated in a tissue-specific manner in bitransgenic mice with this and a tetracycline transactivator (tTA; "tet-off") or reverse transactivator (rtTA; "tet-on") transgene by administration/withdrawal of the tetracycline analog doxycycline. [MGI Ref ID J:109835] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Fluorescent Proteins (Generic GFP)
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as homozygotes. |
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| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Fluorescent Protein Strains
View Fluorescent Protein Strains (139 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Tet Expression Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedResearch Tools
Cell Biology Research
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers)
Immunology and Inflammation Research
Tet Expression Systems (tTA/rtTA Responsive Strains)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Frugier T; Koishi K; Matthaei KI; McLennan IS. 2005. Transgenic mice carrying a tetracycline-inducible, truncated transforming growth factor beta receptor (TbetaRII). Genesis 42(1):1-5. [PubMed: 15828000] [MGI Ref ID J:109835]
Price and Supply Information
| Strain Name: | B6.FVB-Tg(tetO-EGFP,-Tgfbr2)8Mcle/J |
| Stock Number: | 005738 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | Genotype(s) Provided | ||||
|---|---|---|---|---|---|
| Individual Mouse Price | $236.40 | Hemizygous for Tg(tetO-EGFP,-Tgfbr2)8Mcle | |||
| Pair | $288.65 | Hemizygous for Tg(tetO-EGFP,-Tgfbr2)8Mcle x Noncarrier | |||
| Pair | $288.65 | Noncarrier x Hemizygous for Tg(tetO-EGFP,-Tgfbr2)8Mcle | |||
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Histology and Tissue Collection Services are available for all JAX® Mice strains. For more information, please contact Customer Service at orderquest@jax.org or 1-800-422-6423. Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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