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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Heterozygote (Female x Male) 04-OCT-11 Species laboratory mouse Generation N6+N1F12 (27-NOV-11)
Generation DefinitionsDescription
This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.Development
The construct insert is reported to be "LacO-SA-IRES-lacZ-Neo555G/Kan." Upon arrival at The Jackson Laboratory, these mice had been backcrossed at least 6 generations to C57BL/6 mice.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (245 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Adipor2tm1Dgen/Adipor2tm1Dgen
B6.129P2-Adipor2tm1Dgen
- homeostasis/metabolism phenotype
- abnormal hormone level (MGI Ref ID J:122027)
- abnormal lipid homeostasis (MGI Ref ID J:122027)
- decreased circulating cholesterol level
- regardless of diet, plasma cholesterol levels are reduced (MGI Ref ID J:122027)
- decreased circulating HDL cholesterol level (MGI Ref ID J:122027)
- decreased liver triglyceride level
- on normal chow or after a high fat diet, liver triglyceride levels are lower (MGI Ref ID J:122027)
- improved glucose tolerance
- on a high fat diet, glucose response in females is 9% lower than in controls (MGI Ref ID J:122027)
- increased energy expenditure
- decreased susceptibility to diet-induced obesity
- males and females are lean on regular chow and are resistant to high fat diet weight gain (MGI Ref ID J:122027)
- adipose tissue phenotype
- abnormal brown adipose tissue morphology
- decreased brown adipose tissue amount
- after 11 weeks on a high fat diet (MGI Ref ID J:122027)
- abnormal fat cell morphology
- at 8 weeks on a high fat diet, females have smaller adipocytes (MGI Ref ID J:122027)
- decreased gonadal fat pad weight
- after 11 weeks on a high fat diet, males and females have less reproductive white adipose tissue (MGI Ref ID J:122027)
- decreased percent body fat
- at 8 weeks on a high fat diet, females have a lower total body fat mass that controls (MGI Ref ID J:122027)
- decreased renal fat pad weight
- after 11 weeks on a high fat diet, males and females have less perirenal white adipose tissue (MGI Ref ID J:122027)
- decreased white adipose tissue amount
- after 11 weeks on a high fat diet, males and females have less reproductive and perirenal white adipose tissue (MGI Ref ID J:122027)
- behavior/neurological phenotype
- hyperactivity
- during light and dark phase, locomotor activity is increased at the expense of time spent in a corner (MGI Ref ID J:122027)
- increased eating behavior
- 28% increase in food intake (MGI Ref ID J:122027)
- growth/size phenotype
- decreased lean body mass
- at 8 weeks on a high fat diet, females have a moderately lower lean body mass (MGI Ref ID J:122027)
- decreased susceptibility to diet-induced obesity
- males and females are lean on regular chow and are resistant to high fat diet weight gain (MGI Ref ID J:122027)
- nervous system phenotype
- increased brain weight (MGI Ref ID J:122027)
- reproductive system phenotype
- decreased testis weight (MGI Ref ID J:122027)
- endocrine/exocrine gland phenotype
- decreased testis weight (MGI Ref ID J:122027)
- liver/biliary system phenotype
- decreased liver triglyceride level
- on normal chow or after a high fat diet, liver triglyceride levels are lower (MGI Ref ID J:122027)
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Adipor2tm1Dgen/Adipor2tm1Dgen
involves: 129P2/OlaHsd * C57BL/6
- reproductive system phenotype
- abnormal spermatogenesis
- sloughed spermatogenic precursors (MGI Ref ID J:101679)
- oligozoospermia (MGI Ref ID J:101679)
- decreased epididymis weight
- low weight (MGI Ref ID J:101679)
- decreased testis weight
- seminiferous tubule degeneration
- multinucleate giant cells present in the seminiferous tubules (MGI Ref ID J:101679)
- endocrine/exocrine gland phenotype
- decreased testis weight
- seminiferous tubule degeneration
- multinucleate giant cells present in the seminiferous tubules (MGI Ref ID J:101679)
This mouse can be used to support research in many areas including:
Cardiovascular Research
Metabolism Research
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Adipor2tm1Dgen | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Deltagen | ||
| Allele Type | Targeted (Reporter) | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | lacZ expression is detected in: brain, spinal cord, sciatic nerve, eyes, thymus, spleen, lymph nodes, bone marrow, aorta, heart, lung, liver, pancreas, kidney, urinary bladder, thyroid gland, trachea, pituitary gland, adrenal glands, skeletal muscle, skin, testis, prostate gland, ovary, uterus and cervix; with strongest expression detected in the liver. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Molecular Note | A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RNA transcripts are detectable in all tissues analyzed. LacZ expression was detected in all of the organs examined. [MGI Ref ID J:122027] | ||
| Gene Symbol and Name | Adipor2, adiponectin receptor 2 | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | 1110001I14Rik; ACDCR2; AI115388; AW554121; D6Ucla1e; DNA segment, Chr 6, University of California at Los Angeles-1, expressed; EST AI115388; PAQR2; RIKEN cDNA 1110001I14 gene; expressed sequence AW554121; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Adipor2tm1Dgen, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Adipor2tm1Dgen relatedBjursell M; Ahnmark A; Bohlooly-Y M; William-Olsson L; Rhedin M; Peng XR; Ploj K; Gerdin AK; Arnerup G; Elmgren A; Berg AL; Oscarsson J; Linden D. 2007. Opposing effects of adiponectin receptors 1 and 2 on energy metabolism. Diabetes 56(3):583-93. [PubMed: 17327425] [MGI Ref ID J:122027]
Deltagen Inc. 2005. NIH initiative supporting placement of Deltagen, Inc. mice into public repositories MGI Direct Data Submission :. [MGI Ref ID J:101679]
Wang Y; Lau WB; Gao E; Tao L; Yuan Y; Li R; Wang X; Koch WJ; Ma XL. 2010. Cardiomyocyte-derived adiponectin is biologically active in protecting against myocardial ischemia-reperfusion injury. Am J Physiol Endocrinol Metab 298(3):E663-70. [PubMed: 20028965] [MGI Ref ID J:162898]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Mating System Homozygote x Heterozygote (Female x Male) 04-OCT-11 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $155.00 Male Heterozygous for Adipor2tm1Dgen $242.00 Female Homozygous for Adipor2tm1Dgen
Pairs /Price (US dollars $) Pair Genotype $397.00 Homozygous for Adipor2tm1Dgen x Heterozygous for Adipor2tm1Dgen Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $201.50 Male Heterozygous for Adipor2tm1Dgen $314.60 Female Homozygous for Adipor2tm1Dgen
Pairs /Price (US dollars $) Pair Genotype $516.10 Homozygous for Adipor2tm1Dgen x Heterozygous for Adipor2tm1Dgen Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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