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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Heterozygote (Female x Male) 09-DEC-08 Species laboratory mouse Generation N6+N1F6 (08-DEC-08) Description
This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.Development
The construct insert is reported to be "LacO-SA-IRES-lacZ-Neo555G/Kan." Upon arrival at The Jackson Laboratory, these mice had been backcrossed at least 6 generations to C57BL/6 mice.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (186 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
Adipor2tm1Dgen/Adipor2tm1Dgen
B6.129P2-Adipor2tm1Dgen
- homeostasis/metabolism phenotype
- abnormal hormone level (MGI Ref ID J:122027)
- decreased circulating insulin level (MGI Ref ID J:122027)
- on a high fat diet, fasting insulin levels in females are 76% lower
- increased adiponectin level (MGI Ref ID J:122027)
- adiponectin levels are elevated in females on normal chow
- abnormal lipid homeostasis (MGI Ref ID J:122027)
- abnormal triglyceride level (MGI Ref ID J:122027)
- on normal chow or after a high fat diet, liver triglyceride levels are lower
- decreased circulating cholesterol level (MGI Ref ID J:122027)
- regardless of diet, plasma cholesterol levels are reduced
- decreased circulating HDL cholesterol level (MGI Ref ID J:122027)
- improved glucose tolerance (MGI Ref ID J:122027)
- on a high fat diet, glucose response in females is 9% lower than in controls
- increased energy expenditure (MGI Ref ID J:122027)
- energy expenditure is increased
- however, respiratory exchange ratio is unchanged
- increased resistance to diet-induced obesity (MGI Ref ID J:122027)
- males and females are lean on regular chow and are resistant to high fat diet weight gain
- adipose tissue phenotype
- abnormal brown adipose tissue morphology (MGI Ref ID J:122027)
- at 8 weeks on a high fat diet, females brown adipose tissue has less vacuoles
- brown adipose tissue contains more mitochondria
- decreased brown adipose tissue amount (MGI Ref ID J:122027)
- after 11 weeks on a high fat diet
- abnormal fat cell morphology (MGI Ref ID J:122027)
- at 8 weeks on a high fat diet, females have smaller adipocytes
- decreased gonadal fat pad weight (MGI Ref ID J:122027)
- after 11 weeks on a high fat diet, males and females have less reproductive white adipose tissue
- decreased percent body fat (MGI Ref ID J:122027)
- at 8 weeks on a high fat diet, females have a lower total body fat mass that controls
- decreased renal fat pad weight (MGI Ref ID J:122027)
- after 11 weeks on a high fat diet, males and females have less perirenal white adipose tissue
- decreased white adipose tissue amount (MGI Ref ID J:122027)
- after 11 weeks on a high fat diet, males and females have less reproductive and perirenal white adipose tissue
- behavior/neurological phenotype
- hyperactivity (MGI Ref ID J:122027)
- during light and dark phase, locomotor activity is increased at the expense of time spent in a corner
- increased eating behavior (MGI Ref ID J:122027)
- 28% increase in food intake
- growth/size phenotype
- decreased lean body mass (MGI Ref ID J:122027)
- at 8 weeks on a high fat diet, females have a moderately lower lean body mass
- increased resistance to diet-induced obesity (MGI Ref ID J:122027)
- males and females are lean on regular chow and are resistant to high fat diet weight gain
- nervous system phenotype
- increased brain weight (MGI Ref ID J:122027)
- reproductive system phenotype
- decreased testis weight (MGI Ref ID J:122027)
- endocrine/exocrine gland phenotype
- decreased testis weight (MGI Ref ID J:122027)
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Adipor2tm1Dgen/Adipor2tm1Dgen
involves: 129P2/OlaHsd * C57BL/6
- reproductive system phenotype
- abnormal spermatogenesis (MGI Ref ID J:101679)
- sloughed spermatogenic precursors
- oligozoospermia (MGI Ref ID J:101679)
- decreased epididymis weight (MGI Ref ID J:101679)
- low weight
- decreased testis weight (MGI Ref ID J:101679)
- in one of three males examined
- low testis weight in all males tested
- seminiferous tubule degeneration (MGI Ref ID J:101679)
- multinucleate giant cells present in the seminiferous tubules
- endocrine/exocrine gland phenotype
- decreased testis weight (MGI Ref ID J:101679)
- in one of three males examined
- low testis weight in all males tested
- seminiferous tubule degeneration (MGI Ref ID J:101679)
- multinucleate giant cells present in the seminiferous tubules
This mouse can be used to support research in many areas including:
Cardiovascular Research
Metabolism Research
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Adipor2tm1Dgen | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Deltagen | ||
| Allele Type | Targeted (Reporter) | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | lacZ expression is detected in: brain, spinal cord, sciatic nerve, eyes, thymus, spleen, lymph nodes, bone marrow, aorta, heart, lung, liver, pancreas, kidney, urinary bladder, thyroid gland, trachea, pituitary gland, adrenal glands, skeletal muscle, skin, testis, prostate gland, ovary, uterus and cervix; with strongest expression detected in the liver. | ||
| Gene Symbol and Name | Adipor2, adiponectin receptor 2 | ||
| Chromosome | 6 | ||
| Gene Common Name(s) | 1110001I14Rik; ACDCR2; AI115388; AW554121; D6Ucla1e; DNA segment, Chr 6, University of California at Los Angeles-1, expressed; EST AI115388; FLJ21432; MGC4640; PAQR2; RIKEN cDNA 1110001I14 gene; expressed sequence AW554121; | ||
| Molecular Note | A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RNA transcripts are detectable in all tissues analyzed. LacZ expression was detected in all of the organs examined. [MGI Ref ID J:122027] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Adipor2tm1Dgen, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Additional References
Adipor2tm1Dgen relatedBjursell M; Ahnmark A; Bohlooly-Y M; William-Olsson L; Rhedin M; Peng XR; Ploj K; Gerdin AK; Arnerup G; Elmgren A; Berg AL; Oscarsson J; Linden D. 2007. Opposing effects of adiponectin receptors 1 and 2 on energy metabolism. Diabetes 56(3):583-93. [PubMed: 17327425] [MGI Ref ID J:122027]
Deltagen, Inc.. 2005. NIH initiative supporting placement of Deltagen, Inc. mice into public repositories MGI Direct Data Submission :. [MGI Ref ID J:101679]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Mating System Heterozygote x Heterozygote (Female x Male) 09-DEC-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $134.50 Female or Male Heterozygous for Adipor2tm1Dgen
Pairs /Price (US dollars $) Pair Genotype $269.00 Heterozygous for Adipor2tm1Dgen x Heterozygous for Adipor2tm1Dgen
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $174.90 Female or Male Heterozygous for Adipor2tm1Dgen
Pairs /Price (US dollars $) Pair Genotype $349.70 Heterozygous for Adipor2tm1Dgen x Heterozygous for Adipor2tm1Dgen
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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