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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Species laboratory mouse Description
This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.Development
The construct insert is reported to be "IRES-lacZ-Neo555G." Upon arrival at The Jackson Laboratory, these mice had been backcrossed at least 6 generations to C57BL/6 mice.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (174 strains)
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
Mammalian Phenotype Terms assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Capn5tm1Dgen/Capn5tm1Dgen
involves: 129P2/OlaHsd * C57BL/6
- lethality-prenatal/perinatal
- embryonic lethality before implantation (MGI Ref ID J:101679)
- die in utero before E3.5
Research Applications
This mouse can be used to support research in many areas including:
Capn5tm1Dgen relatedResearch Tools
lacZ Expression
Developmental Biology Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Capn5tm1Dgen | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Deltagen | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | lacZ expression is detected in: kidney, urinary bladder, salivary glands and male reproductive system. Strong expression is detected in the brain, spinal cord and ganglia. | ||
| Gene Symbol and Name | Capn5, calpain 5 | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | FLJ46245; HTRA3; nCL-3; | ||
| Molecular Note | A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RNA transcripts are detectable in salivary gland, subcortical region of the brain, cerebellum, brainstem, olfactory bulb, spinal cord, eye, Harderian glands, lung, liver, pancreas, kidney, spleen, thymus, bone marrow, skin, gallbladder, urinary bladder, stomach, large intestine, cecum, epididymis, seminal vesicle, coagulating gland, prostate gland, ovaries and uterus. Striking lacZ (beta-galactosidase) expression is detectable in brain, spinal cord and ganglia. Other tissues displaying X-Gal staining are kidney, urinary bladder, salivary glands, and male reproductive system. | ||
Genotyping
Genotyping Information
Genotyping Protocols
Capn5tm1Dgen, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Additional References
Capn5tm1Dgen related
Deltagen, Inc.. 2005. NIH initiative supporting placement of Deltagen, Inc. mice into public repositories MGI Direct Data Submission :. [MGI Ref ID J:101679]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
Pricing for USA, Canada and Mexico shipping destinations
View International Pricing
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
|
Pricing for International shipping destinations
View USA, Canada and Mexico Pricing
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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