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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 21-OCT-08 Species laboratory mouse Generation N5+N1F7 (24-MAR-11)
Generation DefinitionsDescription
This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.Development
The construct insert is reported to be "SA-IRES-lacZ-Neo555G/Kan." Upon arrival at The Jackson Laboratory, the donating investigator stated that these mice had been backcrossed at least 5 generations to C57BL/6 mice (see SNP note below).A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 2 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (245 strains)
013061 B6.129S6-Ccr6tm1(EGFP)Irw/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Ccr6tm1Dgen/Ccr6tm1Dgen
involves: 129P2/OlaHsd * C57BL/6
- normal phenotype
- no abnormal phenotype detected
- no significant differences in physical examinations, necropsy including body length, body weight, and organ weight measurements, histological examination of tissues and organs, bone marrow section evaluations, complete blood counts and differentials, clinical chemistry panels, densitometry, fertility, or aging studies detected in the homozygous mutant mice when compared with age- and gender-matched wild-type control mice (MGI Ref ID J:101679)
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Ccr6tm1Dgen | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Deltagen | ||
| Allele Type | Targeted (Reporter) | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | lacZ expression mimics endogenous gene expression and is detected in the spleen and lymph nodes. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Molecular Note | A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. While RT-PCR analysis detects a high level of gene transcript in spleen and lymph nodes, and a lower level in lung, pancreas, thymus, bone marrow, skeletal muscle, stomach, small intestine, large intestine, cecum, testis, epididymis, seminal vesicle, coagulating gland and prostate gland, beta-galactosidase expression from this allele is detectable only in spleen and lymph nodes. | ||
| Gene Symbol and Name | Ccr6, chemokine (C-C motif) receptor 6 | ||
| Chromosome | 17 | ||
| Gene Common Name(s) | BN-1; C-C CKR-6; CC-CKR-6; CCR-6; CD196; CKR-L3; CKRL3; CMKBR6; Cmkbr6; DCR2; DRY6; GPR29; GPRCY4; STRL22; carbon catabolite repression 6 homolog (S. cerevisiae); carbon catabolite repression 6 homologue (S. cerevisiae); chemokine (C-C) receptor 6; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Ccr6tm1Dgen, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Ccr6tm1Dgen relatedDeltagen Inc. 2005. NIH initiative supporting placement of Deltagen, Inc. mice into public repositories MGI Direct Data Submission :. [MGI Ref ID J:101679]
Dunay IR; Damatta RA; Fux B; Presti R; Greco S; Colonna M; Sibley LD. 2008. Gr1(+) Inflammatory Monocytes Are Required for Mucosal Resistance to the Pathogen Toxoplasma gondii. Immunity 29(2):306-17. [PubMed: 18691912] [MGI Ref ID J:139568]
Kitamura H; Cambier S; Somanath S; Barker T; Minagawa S; Markovics J; Goodsell A; Publicover J; Reichardt L; Jablons D; Wolters P; Hill A; Marks JD; Lou J; Pittet JF; Gauldie J; Baron JL; Nishimura SL. 2011. Mouse and human lung fibroblasts regulate dendritic cell trafficking, airway inflammation, and fibrosis through integrin alphavbeta8-mediated activation of TGF-beta. J Clin Invest 121(7):2863-75. [PubMed: 21646718] [MGI Ref ID J:175658]
Li Q; Ke F; Zhang W; Shen X; Xu Q; Wang H; Yu XZ; Leng Q; Wang H. 2011. Plasmin plays an essential role in amplification of psoriasiform skin inflammation in mice. PLoS One 6(2):e16483. [PubMed: 21311769] [MGI Ref ID J:170907]
Martin-Orozco N; Muranski P; Chung Y; Yang XO; Yamazaki T; Lu S; Hwu P; Restifo NP; Overwijk WW; Dong C. 2009. T helper 17 cells promote cytotoxic T cell activation in tumor immunity. Immunity 31(5):787-98. [PubMed: 19879162] [MGI Ref ID J:158561]
Paradis TJ; Cole SH; Nelson RT; Gladue RP. 2008. Essential role of CCR6 in directing activated T cells to the skin during contact hypersensitivity. J Invest Dermatol 128(3):628-33. [PubMed: 17882271] [MGI Ref ID J:133473]
Seo KY; Han SJ; Cha HR; Seo SU; Song JH; Chung SH; Kweon MN. 2010. Eye mucosa: an efficient vaccine delivery route for inducing protective immunity. J Immunol 185(6):3610-9. [PubMed: 20709955] [MGI Ref ID J:163825]
Yamazaki T; Yang XO; Chung Y; Fukunaga A; Nurieva R; Pappu B; Martin-Orozco N; Kang HS; Ma L; Panopoulos AD; Craig S; Watowich SS; Jetten AM; Tian Q; Dong C. 2008. CCR6 regulates the migration of inflammatory and regulatory T cells. J Immunol 181(12):8391-401. [PubMed: 19050256] [MGI Ref ID J:142071]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous mice are bred together to produce homozygous, heterozygous, and wildtype offspring. Mating System Homozygote x Homozygote (Female x Male) 21-OCT-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $172.00 Female or Male Homozygous for Ccr6tm1Dgen
Pairs /Price (US dollars $) Pair Genotype $344.00 Homozygous for Ccr6tm1Dgen x Homozygous for Ccr6tm1Dgen Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $223.60 Female or Male Homozygous for Ccr6tm1Dgen
Pairs /Price (US dollars $) Pair Genotype $447.20 Homozygous for Ccr6tm1Dgen x Homozygous for Ccr6tm1Dgen Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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| fax: | 207-288-6655 |
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