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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 08-JAN-08 Species laboratory mouse Generation N5+N1F5 (31-DEC-08) Description
This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.Development
The construct insert is reported to be "IRES-lacZ-Neo555T." Upon arrival at The Jackson Laboratory, these mice had been backcrossed at least 5 generations to C57BL/6 mice.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (186 strains)
006621 B6.129P2(C)-Ccr7tm1Rfor/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Ccr7tm1Dgen/Ccr7tm1Dgen
involves: 129P2/OlaHsd * C57BL/6
- immune system phenotype
- chronic inflammation (MGI Ref ID J:101679)
- at 49 days of age, mice exhibit patchy peribroncheolar mixed lymphocytic infiltrates
- at 49 days of age, pancreatic periductular, and to a lesser extent, peri-islet lymphocytic infiltrates
- at 49 days of age, mild to moderate predominantly lymphocytic gastric cellular infiltrates, involving one or more layers
- at 300 days of age, mild, lymphocytic infiltrates associated primarily with portal triads in the liver
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Ccr7tm1Dgen | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Deltagen | ||
| Allele Type | Targeted (Reporter) | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | lacZ expression is detect in the spleen and lymph nodes and in coagulating glands of the male reproductive system. | ||
| Gene Symbol and Name | Ccr7, chemokine (C-C motif) receptor 7 | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | BLR2; CD197; CDw197; CMKBR7; Cmkbr7; EBI1; Ebi1h; Epstein-Barr virus induced gene 1 homolog; MGC108519; chemokine (C-C) receptor 7; | ||
| Molecular Note | A bacterial lacZ-neomycin fusion gene was inserted into the gene to replace a 608 bp portion of exon 3 of the gene , encoding thr-38 to glu-299, such that the endogenous gene promoter drives expression of beta-galactosidase. Beta-galactosidase expressionfrom this allele is detectable in the immune system, including the spleen and lymph nodes and in coagulating glands of the male reproductive system. [MGI Ref ID J:135850] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Ccr7tm1Dgen, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Additional References
Ccr7tm1Dgen relatedDeltagen, Inc.. 2005. NIH initiative supporting placement of Deltagen, Inc. mice into public repositories MGI Direct Data Submission :. [MGI Ref ID J:101679]
Nitta T; Nitta S; Lei Y; Lipp M; Takahama Y. 2009. CCR7-mediated migration of developing thymocytes to the medulla is essential for negative selection to tissue-restricted antigens. Proc Natl Acad Sci U S A 106(40):17129-33. [PubMed: 19805112] [MGI Ref ID J:153692]
Pahuja A; Maki RA; Hevezi PA; Chen A; Verge GM; Lechner SM; Roth RB; Zlotnik A; Alleva DG. 2006. Experimental autoimmune encephalomyelitis develops in CC chemokine receptor 7-deficient mice with altered T-cell responses. Scand J Immunol 64(4):361-9. [PubMed: 16970675] [MGI Ref ID J:135850]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Mating System Homozygote x Homozygote (Female x Male) 08-JAN-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $126.00 Female or Male Homozygous for Ccr7tm1Dgen
Pairs /Price (US dollars $) Pair Genotype $252.00 Homozygous for Ccr7tm1Dgen x Homozygous for Ccr7tm1Dgen
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $163.80 Female or Male Homozygous for Ccr7tm1Dgen
Pairs /Price (US dollars $) Pair Genotype $327.60 Homozygous for Ccr7tm1Dgen x Homozygous for Ccr7tm1Dgen
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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| fax: | 207-288-6655 |
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