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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 08-JAN-08 Species laboratory mouse Generation N5+N1F10 (24-MAR-11)
Generation DefinitionsDescription
This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.Development
The construct insert is reported to be "IRES-lacZ-Neo555T." Upon arrival at The Jackson Laboratory, these mice had been backcrossed at least 5 generations to C57BL/6 mice.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (245 strains)
006621 B6.129P2(C)-Ccr7tm1Rfor/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Ccr7tm1Dgen/Ccr7tm1Dgen
involves: 129P2/OlaHsd * C57BL/6
- immune system phenotype
- chronic inflammation
- at 49 days of age, mice exhibit patchy peribroncheolar mixed lymphocytic infiltrates (MGI Ref ID J:101679)
- at 49 days of age, pancreatic periductular, and to a lesser extent, peri-islet lymphocytic infiltrates (MGI Ref ID J:101679)
- at 49 days of age, mild to moderate predominantly lymphocytic gastric cellular infiltrates, involving one or more layers (MGI Ref ID J:101679)
- at 300 days of age, mild, lymphocytic infiltrates associated primarily with portal triads in the liver (MGI Ref ID J:101679)
This mouse can be used to support research in many areas including:
Immunology and Inflammation Research
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Ccr7tm1Dgen | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Deltagen | ||
| Allele Type | Targeted (Reporter) | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | lacZ expression is detect in the spleen and lymph nodes and in coagulating glands of the male reproductive system. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Molecular Note | A bacterial lacZ-neomycin fusion gene was inserted into the gene to replace a 608 bp portion of exon 3 of the gene , encoding thr-38 to glu-299, such that the endogenous gene promoter drives expression of beta-galactosidase. Beta-galactosidase expressionfrom this allele is detectable in the immune system, including the spleen and lymph nodes and in coagulating glands of the male reproductive system. [MGI Ref ID J:135850] | ||
| Gene Symbol and Name | Ccr7, chemokine (C-C motif) receptor 7 | ||
| Chromosome | 11 | ||
| Gene Common Name(s) | BLR2; CD197; CDw197; CMKBR7; Cmkbr7; EBI1; Ebi1h; Epstein-Barr virus induced gene 1 homolog; chemokine (C-C) receptor 7; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Ccr7tm1Dgen, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Ccr7tm1Dgen relatedCoghill JM; Carlson MJ; Panoskaltsis-Mortari A; West ML; Burgents JE; Blazar BR; Serody JS. 2010. Separation of graft-versus-host disease from graft-versus-leukemia responses by targeting CC-chemokine receptor 7 on donor T cells. Blood 115(23):4914-22. [PubMed: 20185583] [MGI Ref ID J:161567]
Deltagen Inc. 2005. NIH initiative supporting placement of Deltagen, Inc. mice into public repositories MGI Direct Data Submission :. [MGI Ref ID J:101679]
Demoor T; Bracke KR; Vermaelen KY; Dupont L; Joos GF; Brusselle GG. 2009. CCR7 modulates pulmonary and lymph node inflammatory responses in cigarette smoke-exposed mice. J Immunol 183(12):8186-94. [PubMed: 19923454] [MGI Ref ID J:157502]
Nitta T; Nitta S; Lei Y; Lipp M; Takahama Y. 2009. CCR7-mediated migration of developing thymocytes to the medulla is essential for negative selection to tissue-restricted antigens. Proc Natl Acad Sci U S A 106(40):17129-33. [PubMed: 19805112] [MGI Ref ID J:153692]
Pahuja A; Maki RA; Hevezi PA; Chen A; Verge GM; Lechner SM; Roth RB; Zlotnik A; Alleva DG. 2006. Experimental autoimmune encephalomyelitis develops in CC chemokine receptor 7-deficient mice with altered T-cell responses. Scand J Immunol 64(4):361-9. [PubMed: 16970675] [MGI Ref ID J:135850]
Zlotoff DA; Zhang SL; De Obaldia ME; Hess PR; Todd SP; Logan TD; Bhandoola A. 2011. Delivery of progenitors to the thymus limits T-lineage reconstitution after bone marrow transplantation. Blood 118(7):1962-70. [PubMed: 21659540] [MGI Ref ID J:176932]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number AX12
Colony Maintenance
Mating System Homozygote x Homozygote (Female x Male) 08-JAN-08 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $155.00 Female or Male Homozygous for Ccr7tm1Dgen
Pairs /Price (US dollars $) Pair Genotype $310.00 Homozygous for Ccr7tm1Dgen x Homozygous for Ccr7tm1Dgen Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
| Pricing for International shipping destinations |
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Live Mice
Price (US dollars $) Gender Genotypes Provided Individual Mouse $201.50 Female or Male Homozygous for Ccr7tm1Dgen
Pairs /Price (US dollars $) Pair Genotype $403.00 Homozygous for Ccr7tm1Dgen x Homozygous for Ccr7tm1Dgen Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
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Standard Supply
Repository-Live. The Repository Strains represent an exclusive set of over 1500 unique mouse models maintained at The Jackson Laboratory to support a vast array of research areas. The breeding colonies for Repository Strains provide mice for both large and small orders and fluctuate in size depending on current demand for each strain. We treat orders for these strains as custom orders. Within 2 business days, we respond to each availability inquiry or order with various delivery options. Repository Strains typically are delivered at 4 to 8 weeks of age and will not exceed 12 weeks of age on the day of shipping.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
Control Information
| Control | ||
|---|---|---|
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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