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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation ?+N1p (22-JAN-06) Description
This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.Development
The construct insert is reported to be "SA-IRES-lacZ-Neo555G/Kan." Upon arrival at The Jackson Laboratory, these mice had been backcrossed at least 6 generations to C57BL/6 mice.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (186 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Faim2tm1Dgen/Faim2+
involves: 129P2/OlaHsd * C57BL/6
- renal/urinary system phenotype
- hydronephrosis (MGI Ref ID J:101679)
- mild unilateral renal pelvic dilation was present at 49 days in two heterozygous females
Faim2tm1Dgen/Faim2tm1Dgen
involves: 129P2/OlaHsd * C57BL/6
- renal/urinary system phenotype
- abnormal kidney morphology (MGI Ref ID J:101679)
- unspecified kidney lesions related to genotype were present in homozygous mice
- enlarged kidney (MGI Ref ID J:101679)
- 49 day homozygous females, 300 day homozygous males
- increased kidney weight (MGI Ref ID J:101679)
- weight increased, 49 day homozygous female and males
- weight to body weight ratio increased, 49 day homozygous female and male, and 300 day homozygous female and male
- hydronephrosis (MGI Ref ID J:101679)
- moderate to severe bilateral renal pelvic dilation was present at 49 days in two homozygous females and a homozygous male, and at 300 days in two homozygous males
- minimal or moderate unilateral renal pelvic dilation was present at 300 days in homozygous females
- behavior/neurological phenotype
- increased susceptibility to pharmacologically induced seizures (MGI Ref ID J:101679)
- homozygotes required a lower dose of metrazol to induce tonic/clonic seizure and death in the metrazol test
- nervous system phenotype
- increased susceptibility to pharmacologically induced seizures (MGI Ref ID J:101679)
- homozygotes required a lower dose of metrazol to induce tonic/clonic seizure and death in the metrazol test
This mouse can be used to support research in many areas including:
Internal/Organ Research
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Faim2tm1Dgen | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Deltagen | ||
| Allele Type | Targeted (Reporter) | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | lacZ expression is detected in the brain. | ||
| Gene Symbol and Name | Faim2, Fas apoptotic inhibitory molecule 2 | ||
| Chromosome | 15 | ||
| Gene Common Name(s) | 2900002L20Rik; AI854036; KIAA0950; LFG; MGC105316; NGP35; NMP25; NMP35; RIKEN cDNA 2900002L20 gene; TMBIM2; expressed sequence AI854036; lifeguard; mKIAA0950; | ||
| Molecular Note | A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RT-PCR analysis detects gene transcript in brain, cortex, subcortical region, cerebellum, brainstem, olfactory bulb, spinal cord, eye, heart, lung, pancreas, kidney, thymus, lymph nodes, bone marrow, skin, gallbladder, urinary bladder, pituitary gland, adrenal gland, salivary gland, skeletal muscle, tongue, stomach, small intestine, large intestine, cecum, testis, epididymis, seminal vesicle, coagulating gland, prostate gland, ovaries, uterus and white fat. Beta-galactosidase expression from this allele is detectable in brain. In wholemount staining lacZ expression is detectable in olfactory bulb, cortex, thalamus, hypothalamus and cerebellum. On coronal sections lacZ expression is detectable in cortex and hippocampus. | ||
Genotyping
Genotyping Information
Genotyping Protocols
Faim2tm1Dgen, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Additional References
Faim2tm1Dgen relatedDeltagen, Inc.. 2005. NIH initiative supporting placement of Deltagen, Inc. mice into public repositories MGI Direct Data Submission :. [MGI Ref ID J:101679]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 Cryopreserved Embryos $1600.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 Cryopreserved Embryos $2080.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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For Licensing and Use Restrictions view the link(s) below:
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Contact information
General inquiries
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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