Strain Name: |
B6.129P2-Fzd1tm1Dgen/J |
|---|---|
Stock Number: |
005803 |
Availability: | Repository-Cryopreserved |
Price and Supply Information | |
General Terms and Conditions |
| Genes & Alleles | Fzd1; Fzd1tm1Dgen; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Strain Description
This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.
Initial reports from Delatagen state that homozygous animals are viable and fertile. However, it is the experience of The Jackson Laboratory that homozygous animals are not recovered from heterozygous matings. More that 100 progeny from heterozygous matings were genotyped.Strain Development
The construct insert is reported to be "SA-IRES-lacZ-Neo555G/Kan." Upon arrival at The Jackson Laboratory, these mice had been backcrossed at least 6 generations to C57BL/6 mice.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Fzd1tm1Dgen | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Deltagen | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | lacZ expression is detected in most tissues examined: brain, spinal cord, sciatic nerve, eyes, thymus, spleen, lymph nodes, aorta, heart, lung, liver, pancreas, kidney, urinary bladder, thyroid gland, trachea, pituitary gland, adrenal glands, skeletal muscle, skin, testis, prostate gland and ovary. Strong expression is detected in the CNS, muscularis of the urinary bladder and blood vessels. | ||
| Gene Symbol and Name | Fzd1, frizzled homolog 1 (Drosophila) | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | AW227548; DKFZp564G072; FZ-1; expressed sequence AW227548; | ||
| Molecular Note | A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RT-PCR analysis detects a high level of gene transcript in lung, kidney, skin, gallbladder, urinary bladder, pituitary gland,adrenal gland, colon, epididymis, seminal vesicle, coagulating gland, prostate gland, ovary, uterus and white fat and low levels in whole brain, cortex, subcortical region, cerebellum, brainstem, olfactory bulb, spinal cord, eye, Harderian glands, heart,liver, pancreas, spleen, thymus, lymph nodes, bone marrow, salivary gland, skeletal muscle, tongue, stomach, small intestine, cecum and testis. Beta-galactosidase expression from this allele is detectable in most organs examined. Most striking expression was detected in the central nervous system, muscularis of the urinary bladder and blood vessels. Staining was observed in different tissue and cell types, including cardiac muscle, epithelium, neurons, cartilage, adipose tissue and blood vessels. | ||
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for JAX® GEMM® Strains | ||
Genotyping Protocols
Fzd1tm1Dgen
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (174 strains)
Additional Web Information
Congenic Nomenclature
Fluorescent Proteins/lacZ Systems
Research Applications
This mouse can be used to support research in many areas including:
Fzd1tm1Dgen relatedResearch Tools
lacZ Expression
Developmental Biology Research
References
Additional ReferencesPrice and Supply Information
| Strain Name: | B6.129P2-Fzd1tm1Dgen/J |
| Stock Number: | 005803 |
Price Details
IMPORTANT NOTE: Prices are based on shipping destination. The shipping destinations are:
- USA, Canada and Mexico
- International
*Pricing for Shipping Destination selected:
USA, Canada and Mexico
| Price(s) in US dollars ($) | |||||
|---|---|---|---|---|---|
| Cryorecovery Fee | $1900.00 | ||||
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. View Control Pricing Information for JAX® Strains. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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