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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation ?+N1p Description
This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.Development
The construct insert is reported to be "Lac0-SA-IRES-lacZ-Neo555G/Kan." Upon arrival, these mice had been backcrossed four generations to C57BL/6. During the importation process at The Jackson Laboratory, at least one additional backcross to C57BL/6J mice was performed. The resulting mice are backcrossed a total of at least five generations.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (186 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Ptch2tm1Dgen/Ptch2tm1Dgen
involves: 129P2/OlaHsd * C57BL/6
- hematopoietic system phenotype
- abnormal bone marrow cell number (MGI Ref ID J:101679)
- at 49 days of age the bone marrow myeloid:erythroid ratio is increased in homozygous males
- abnormal red blood cell (MGI Ref ID J:101679)
- mild red blood cell abnormalities including anisocytosis, poikilocytosis, and polychromasia are seen in homozygous males at 49 days of age
- anemia (MGI Ref ID J:101679)
- in homozygous males at 49 days of age hemoglobin, hematocrit, and red blood cell count are decreased
- decreased hematocrit (MGI Ref ID J:101679)
- seen in homozygous males at 49 days of age
- decreased hemoglobin content (MGI Ref ID J:101679)
- seen in homozygous males at 49 days of age
- enlarged spleen (MGI Ref ID J:101679)
- at 49 days of age spleen size, weight, and spleen weight to body weight ratio are increased in homozygous males
- extramedullary hematopoiesis (MGI Ref ID J:101679)
- a moderate to severe diffuse increase in extramedullary hematopoiesis is seen in homozygous males at 49 days of age
- increased neutrophil cell number (MGI Ref ID J:101679)
- the percentage of neutrophils is mildly to moderately increased in homozygous males at 49 days of age
- immune system phenotype
- enlarged spleen (MGI Ref ID J:101679)
- at 49 days of age spleen size, weight, and spleen weight to body weight ratio are increased in homozygous males
- increased neutrophil cell number (MGI Ref ID J:101679)
- the percentage of neutrophils is mildly to moderately increased in homozygous males at 49 days of age
This mouse can be used to support research in many areas including:
Ptch2tm1Dgen relatedResearch Tools
lacZ Expression
Developmental Biology Research
Hematological Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Ptch2tm1Dgen | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Deltagen | ||
| Allele Type | Targeted (Reporter) | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | lacZ expression is detected in: brain, spinal cord, sciatic nerve, aorta, heart, lung, liver, pancreas, kidney, urinary bladder, thyroid gland, trachea, pituitary gland, adrenal glands, skeletal muscle, skin, testis, prostate gland, ovary, uterus and cervix. | ||
| Gene Symbol and Name | Ptch2, patched homolog 2 | ||
| Chromosome | 4 | ||
| Gene Common Name(s) | PTC2; | ||
| Molecular Note | A bacterial lacZ gene was inserted into the gene such that the endogenous gene promoter drives expression of beta-galactosidase. RT-PCR analysis detects a gene transcript at high levels in testis and at lower levels in skin, urinary bladder, pituitary gland, adrenal gland, epididymis, coagulating gland, white fat, and most othe tissues examined; beta-galactosidase expression is detectable in brain, spinal cord, sciatic nerve, aorta, heart, lung, liver, pancreas, kidney, urinary bladder, thyroid gland, trachea, pituitary gland, adrenal glands, skeletal muscle, skin, testis, prostate gland, ovary, uterus and cervix. | ||
Genotyping
Genotyping Information
Genotyping Protocols
Ptch2tm1Dgen, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Additional References
Ptch2tm1Dgen relatedDeltagen, Inc.. 2005. NIH initiative supporting placement of Deltagen, Inc. mice into public repositories MGI Direct Data Submission :. [MGI Ref ID J:101679]
Passman JN; Dong XR; Wu SP; Maguire CT; Hogan KA; Bautch VL; Majesky MW. 2008. A sonic hedgehog signaling domain in the arterial adventitia supports resident Sca1+ smooth muscle progenitor cells. Proc Natl Acad Sci U S A 105(27):9349-54. [PubMed: 18591670] [MGI Ref ID J:137825]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Contact information
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