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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation ?+N1p (29-JAN-06) Description
This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.Development
The construct insert is reported to be "Lac0-SA-IRES-lacZ-Neo555G/Kan." Upon arrival at The Jackson Laboratory, these mice had been backcrossed at least 5 generations to C57BL/6 mice.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (186 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Slc22a6tm1Dgen/Slc22a6tm1Dgen
involves: 129P2/OlaHsd * C57BL/6
- immune system phenotype
- increased thymus weight (MGI Ref ID J:101679)
- all homozygous males have increased thymus weight and thymus to body weight ratio, while females have slightly increased thymus to body weight ratio
- hematopoietic system phenotype
- increased thymus weight (MGI Ref ID J:101679)
- all homozygous males have increased thymus weight and thymus to body weight ratio, while females have slightly increased thymus to body weight ratio
Slc22a6tm1Dgen/Slc22a6tm1Dgen
involves: 129P2/OlaHsd * C57BL/6J
- renal/urinary system phenotype
- abnormal kidney organic anion excretion (MGI Ref ID J:108655)
- in vivo and renal slice excretion of para-aminohippurate (PAH) is significantly reduced; however, excretion of estrone sulfate, inulin clearance, blood pressure, heart rate and kidney morphology are all normal
- also, no differences are seen in body weight or in plasma or urinary electrolyte, creatinine, or glucose levels and histology of the kidneys, heart, liver, spleen, muscle, and brain appears normal
- higher concentrations of furosemide are required to induce similar levels natriuresis compared to wild-type mice
- homeostasis/metabolism phenotype
- decreased physiological sensitivity to xenobiotics (MGI Ref ID J:108655)
- higher concentrations of furosemide are required to induce similar levels natriuresis compared to wild-type mice
Slc22a6tm1Dgen/Slc22a6tm1Dgen
involves: 129P2/OlaHsd
- renal/urinary system phenotype
- abnormal urine homeostasis (MGI Ref ID J:136005)
- the level of urinary urate is decreased 30% compared to in wild-type mice
- homeostasis/metabolism phenotype
- abnormal urine homeostasis (MGI Ref ID J:136005)
- the level of urinary urate is decreased 30% compared to in wild-type mice
This mouse can be used to support research in many areas including:
Slc22a6tm1Dgen relatedResearch Tools
lacZ Expression
Cell Biology Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Slc22a6tm1Dgen | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Deltagen | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Oat1-; | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | lacZ expression is detected in the brain and kidney. | ||
| Gene Symbol and Name | Slc22a6, solute carrier family 22 (organic anion transporter), member 6 | ||
| Chromosome | 19 | ||
| Gene Common Name(s) | FLJ55736; HOAT1; MGC124962; MGC45260; NKT; OAT1; Orctl1; PAHT; ROAT1; mOat1; organic cationic transporter-like 1; | ||
| Molecular Note | A bacterial lacZ gene and neomycin resistance cassette was inserted to replace a 52 bp segment within the coding portion of exon 1 such that the endogenous gene promoter drives expression of beta-galactosidase. While RT-PCR analysis detects high levels of RNA transcripts in the kidney and very low levels in the brain, beta-galactosidase expression from this allele is detectable in brain and kidney. [MGI Ref ID J:108655] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Slc22a6tm1Dgen, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Additional References
Slc22a6tm1Dgen relatedDeltagen, Inc.. 2005. NIH initiative supporting placement of Deltagen, Inc. mice into public repositories MGI Direct Data Submission :. [MGI Ref ID J:101679]
Eraly SA; Vallon V; Rieg T; Gangoiti JA; Wikoff WR; Siuzdak G; Barshop BA; Nigam SK. 2008. Multiple organic anion transporters contribute to net renal excretion of uric acid. Physiol Genomics 33(2):180-92. [PubMed: 18270321] [MGI Ref ID J:136005]
Eraly SA; Vallon V; Vaughn DA; Gangoiti JA; Richter K; Nagle M; Monte JC; Rieg T; Truong DM; Long JM; Barshop BA; Kaler G; Nigam SK. 2006. Decreased renal organic anion secretion and plasma accumulation of endogenous organic anions in OAT1 knock-out mice. J Biol Chem 281(8):5072-83. [PubMed: 16354673] [MGI Ref ID J:108655]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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