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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation ?+N1p (29-JAN-06)
Generation DefinitionsDescription
This targeted mutant was created and characterized by Deltagen, Inc. View phenotypic data developed by Deltagen.Development
The construct insert is reported to be "Lac0-SA-IRES-lacZ-Neo555G/Kan." Upon arrival at The Jackson Laboratory, these mice had been backcrossed at least 5 generations to C57BL/6 mice.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (256 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Slc22a6tm1Dgen/Slc22a6tm1Dgen
involves: 129P2/OlaHsd * C57BL/6
- immune system phenotype
- increased thymus weight
- all homozygous males have increased thymus weight and thymus to body weight ratio, while females have slightly increased thymus to body weight ratio (MGI Ref ID J:101679)
- hematopoietic system phenotype
- increased thymus weight
- all homozygous males have increased thymus weight and thymus to body weight ratio, while females have slightly increased thymus to body weight ratio (MGI Ref ID J:101679)
Slc22a6tm1Dgen/Slc22a6tm1Dgen
involves: 129P2/OlaHsd * C57BL/6J
- renal/urinary system phenotype
- abnormal urine organic anion level
- in vivo and renal slice excretion of para-aminohippurate (PAH) is significantly reduced; however, excretion of estrone sulfate, inulin clearance, blood pressure, heart rate and kidney morphology are all normal (MGI Ref ID J:108655)
- also, no differences are seen in body weight or in plasma or urinary electrolyte, creatinine, or glucose levels and histology of the kidneys, heart, liver, spleen, muscle, and brain appears normal (MGI Ref ID J:108655)
- higher concentrations of furosemide are required to induce similar levels natriuresis compared to wild-type mice (MGI Ref ID J:108655)
- homeostasis/metabolism phenotype
- abnormal urine organic anion level
- in vivo and renal slice excretion of para-aminohippurate (PAH) is significantly reduced; however, excretion of estrone sulfate, inulin clearance, blood pressure, heart rate and kidney morphology are all normal (MGI Ref ID J:108655)
- also, no differences are seen in body weight or in plasma or urinary electrolyte, creatinine, or glucose levels and histology of the kidneys, heart, liver, spleen, muscle, and brain appears normal (MGI Ref ID J:108655)
- higher concentrations of furosemide are required to induce similar levels natriuresis compared to wild-type mice (MGI Ref ID J:108655)
- decreased physiological sensitivity to xenobiotic
- higher concentrations of furosemide are required to induce similar levels natriuresis compared to wild-type mice (MGI Ref ID J:108655)
Slc22a6tm1Dgen/Slc22a6tm1Dgen
involves: 129P2/OlaHsd
- renal/urinary system phenotype
- decreased urine uric acid level
- the level of urinary urate is decreased 30% compared to in wild-type mice (MGI Ref ID J:136005)
- homeostasis/metabolism phenotype
- decreased urine uric acid level
- the level of urinary urate is decreased 30% compared to in wild-type mice (MGI Ref ID J:136005)
This mouse can be used to support research in many areas including:
Slc22a6tm1Dgen relatedResearch Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Slc22a6tm1Dgen | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Deltagen | ||
| Allele Type | Targeted (Reporter) | ||
| Common Name(s) | Oat1-; | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14 | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Site of Expression | lacZ expression is detected in the brain and kidney. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Molecular Note | A bacterial lacZ gene and neomycin resistance cassette was inserted to replace a 52 bp segment within the coding portion of exon 1 such that the endogenous gene promoter drives expression of beta-galactosidase. While RT-PCR analysis detects high levels of RNA transcripts in the kidney and very low levels in the brain, beta-galactosidase expression from this allele is detectable in brain and kidney. [MGI Ref ID J:108655] | ||
| Gene Symbol and Name | Slc22a6, solute carrier family 22 (organic anion transporter), member 6 | ||
| Chromosome | 19 | ||
| Gene Common Name(s) | HOAT1; NKT; OAT1; Orctl1; PAHT; ROAT1; mOat1; organic cationic transporter-like 1; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Slc22a6tm1Dgen, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Additional References
Slc22a6tm1Dgen relatedDeltagen Inc. 2005. NIH initiative supporting placement of Deltagen, Inc. mice into public repositories MGI Direct Data Submission :. [MGI Ref ID J:101679]
Eraly SA; Vallon V; Rieg T; Gangoiti JA; Wikoff WR; Siuzdak G; Barshop BA; Nigam SK. 2008. Multiple organic anion transporters contribute to net renal excretion of uric acid. Physiol Genomics 33(2):180-92. [PubMed: 18270321] [MGI Ref ID J:136005]
Eraly SA; Vallon V; Vaughn DA; Gangoiti JA; Richter K; Nagle M; Monte JC; Rieg T; Truong DM; Long JM; Barshop BA; Kaler G; Nigam SK. 2006. Decreased renal organic anion secretion and plasma accumulation of endogenous organic anions in OAT1 knock-out mice. J Biol Chem 281(8):5072-83. [PubMed: 16354673] [MGI Ref ID J:108655]
Kohler JJ; Hosseini SH; Green E; Abuin A; Ludaway T; Russ R; Santoianni R; Lewis W. 2011. Tenofovir renal proximal tubular toxicity is regulated By OAT1 and MRP4 transporters. Lab Invest 91(6):852-8. [PubMed: 21403643] [MGI Ref ID J:172002]
Torres AM; Dnyanmote AV; Bush KT; Wu W; Nigam SK. 2011. Deletion of multispecific organic anion transporter Oat1/Slc22a6 protects against mercury-induced kidney injury. J Biol Chem 286(30):26391-5. [PubMed: 21652719] [MGI Ref ID J:175373]
Vallon V; Eraly SA; Rao SR; Gerasimova M; Rose M; Nagle M; Anzai N; Smith T; Sharma K; Nigam SK; Rieg T. 2012. A role for the organic anion transporter OAT3 in renal creatinine secretion in mice. Am J Physiol Renal Physiol 302(10):F1293-9. [PubMed: 22338083] [MGI Ref ID J:184733]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2450.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3185.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
JAX® Mice
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For Licensing and Use Restrictions view the link(s) below:
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Contact information
General inquiries regarding Terms of UseContracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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