Strain Name:

NOD.Cg-Tg(HLA-A2/H2-K)1Scr/ShrmJ

Stock Number:

005853

Availability:

Repository-Cryopreserved

Use Restrictions Apply, see Terms of Use
Common Names: NOD CyA2/Kb;     NOD.A2/Kb;    

Description

Strain Information

Type Congenic; Mutant Strain; Transgenic;
Additional information on Genetically Engineered Mutant Mice.
Specieslaboratory mouse
 
Donating Investigator Linda Sherman,   The Scripps Research Institute

Appearance
albino, pink eyed
Related Genotype: A/A Tyrc/Tyrc

Description
Transgenic mice are viable, fertile, normal in size and do not display any behavioral abnormalities. FACs analysis indicates expression of HLA-A201 on peripheral blood lymphocytes. The level of expression of this chimeric MHC I molecule in transgenic mice is similar to the observed expression in human PBL's. Diabetes incidence of mice carrying this transgene (88%) is similar to NOD (79%) at 25 weeks of age. Splenocytes from transgenic mice immunized with GAD65 peptides together with MHC class II helper peptide were re-stimulated with peptide pulsed irradiated syngeneic APC?s in-vitro 10 days post immunization Transgenic mice immunized with three different GAD65 peptides (position: 144-149, sequence: LLQEYNWEL; position: 114-122, sequence: VMNILLQYV; and position 573-581, sequence: FLIEEIERL) gave vigorous CTL responses when tested by standard chromium release assays. FACs analysis indicates presence of CFSE expression on CD8+ T-cells in transgenic mice given CFSE-labeled CD8+ T cells in the pancrease, but not in the peripheral or pancreatic lymph nodes 4 days post injection. CFSE-positive CD8+ T-cells were absent in the pancreas, peripheral and pancreatic lymph nodes of wildtype mice. This model provides a tool to identify MHC class I epitopes recognized by CD8+ T cells in type 1 diabetes patients, in addition to testing new therapies focusing on restoring immune tolerance.

Development
NOD mice carrying a CD8+ T-cell transgene (HLA-A2/H2-K)1Scr express the alpha 1 and alpha2 domain of the human class 1 molecule, HLA-A201, fused to the alpha 3, transmembrane and cytoplasmic domains of the mouse H-2kb molecule. This transgene was injected into C57BL/6J oocytes. Resulting progeny were backcrossed to NOD for 14 generations. In 2006, The Jackson Laboratory received NOD.Cg-Tg(HLA-A2/H2-K)1Scr/ShrmJ at generation N14.

Control Information

  Control
   Noncarrier
   001976 NOD/ShiLtJ (approximate)
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of H2-K     (9 strains)

View Strains carrying other alleles of HLA-A     (9 strains)

Additional Web Information

Congenic Nomenclature
Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Diabetes and Obesity Research
Type 1 Diabetes (IDDM) Analysis Strains (NOD Transgenics)

Immunology and Inflammation Research
Autoimmunity (Type 1 Diabetes)
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Vaccine Development

HLA-A related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Vaccine Development

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(HLA-A2/H2-K)1Scr
Allele Name transgene insertion 1, The Scripps Research Institute
Allele Type Transgenic (random, expressed)
Common Name(s) CyHLA A2.1/Kb;
Mutation Made By Linda Sherman,   The Scripps Research Institute
Strain of OriginC57BL/6J
Expressed Gene H2-K, histocompatibility 2, K region, mouse, laboratory
Promoter HLA-A, major histocompatibility complex, class I, A, human
Molecular Note The transgene expresses a human-mouse chimeric major histocompatibility class I protein composed of the leader sequence and the alpha1 and alpha2 domains of HLA-A2.1 joined to the alpha3, cytoplasmic and transmembrane domains of H2-Kb. Mice expressing this transgene exhibit HLA-A2.1-restricted CD8 T-cell responses to appropriate antigens. [MGI Ref ID J:91744]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Vitiello A; Marchesini D; Furze J; Sherman LA; Chesnut RW. 1991. Analysis of the HLA-restricted influenza-specific cytotoxic T lymphocyte response in transgenic mice carrying a chimeric human-mouse class I major histocompatibility complex. J Exp Med 173(4):1007-15. [PubMed: 1706750]  [MGI Ref ID J:108157]

Additional References

Tg(HLA-A2/H2-K)1Scr related

Dominguez AL; Lustgarten J. 2008. Implications of aging and self-tolerance on the generation of immune and antitumor immune responses. Cancer Res 68(13):5423-31. [PubMed: 18593945]  [MGI Ref ID J:138875]

Irwin MJ; Heath WR; Sherman LA. 1989. Species-restricted interactions between CD8 and the alpha 3 domain of class I influence the magnitude of the xenogeneic response. J Exp Med 170(4):1091-101. [PubMed: 2477484]  [MGI Ref ID J:91744]

Sherman LA. 2004. C57BL/6J mice with MHC class I-H2 chimeric transgene can be used to identify HLA-A2.1 restricted peptide epitopes MGI Direct Data Submission :.  [MGI Ref ID J:91745]

Smith C; Martinez M; Cooper L; Rist M; Zhong J; Khanna R. 2008. Generating functional CD8(+) T cell memory response under transient CD4(+) T cell deficiency: Implications for vaccination of immunocompromised individuals. Eur J Immunol 38(7):1857-66. [PubMed: 18506880]  [MGI Ref ID J:137312]

Zhang Y; Li S; Shan M; Pan X; Zhuang K; He L; Gould K; Tien P. 2007. Hepatitis B virus core antigen epitopes presented by HLA-A2 single-chain trimers induce functional epitope-specific CD8+ T-cell responses in HLA-A2.1/Kb transgenic mice. Immunology 121(1):105-12. [PubMed: 17244158]  [MGI Ref ID J:122636]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Additional Supply Details

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Type 1 Diabetes Repository collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   Noncarrier
   001976 NOD/ShiLtJ (approximate)
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


See Terms of Use


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:207-288-6470
fax:207-288-6655

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