Strain Name: |
STOCK Tg(Cp-EGFP)25Gaia/J |
|---|---|
Stock Number: |
005854 |
Availability: | Repository- Live |
General Terms and Conditions |
| Genes & Alleles | GFP; C; Tg(Cp-EGFP)25Gaia; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Mating System +/+ sibling x Hemizygote (Female x Male) Species laboratory mouse Donating Investigator Nicholas Gaiano, Johns Hopkins Univ School of Medicine Generation N4+F3 (28-DEC-07) Strain Description
Mice homozygous for the Notch reporter transgene are viable, fertile, normal in size, and do not display any behavioral abnormalities. Enhanced green fluorescent protein (EGFP) expression is present in a wide variety of hemizygous cell/tissue types during development and in the adult, including enriched hematopoietic stem cell (HSC) populations. The location of EGFP expression is consistent with Notch signaling pathway elements/genes and appears to faithfully reflect canonical (CBF1-mediated) Notch activity. With respect to hematopoiesis, low expression is shown in fully differentiated cells of the peripheral lymphoid organs (blood and spleen). Isolated HSC retain their ability to differentiate. Mice expressing this Notch reporter transgene may be useful in studying HSC populations and other cell types utilizing the Notch, CBF1, or Wnt signaling pathways. As immature (double negative [DN]) thymocytes have differential expression patterns as they progress from DN1-DN4, these mice may also be useful in thymocyte maturation studies.In addition to the above description, the donating investigator confirms the following characteristics: EGFP expression can be upregulated in transgenic mouse embryonic fibroblasts after infection with activated Notch1-3, or an activated form of CBF1 (CBF1-VP16). In addition, EGFP expression can be inhibited by shRNA-mediated knockdown of CBF1, or by treatment with a gamma-secretase inhibitor. In the developing nervous system, EGFP is expressed in the germinal zone of the brain and spinal cord, and can be used to enrich for neural progenitors by fluorescence-activated cell sorting (FACS). EGFP expression is also present in the adult nervous system, in germinal zones, and also in mature neurons. Isolated hematopoietic stem cells (HSC) retain their ability to differentiate. This line has also been used in published studies examining angiogenesis and hair follicle formation.
Strain Development
An enhanced green fluorescent protein (EGFP) sequence was placed under the control of 4 tandem copies of the C promoter (Cp) binding factor 1 (CBF1) binding site consensus sequence (derived from Epstein Barr Virus) upstream of the SV40 basal promoter. This construct was injected into (B6SJLF1/J x B6SJLF1/J) pronuclei. Male founder #25 was obtained and bred with a female CD1 mouse. Hemizygous mice were bred with CD1 for three generations before being made homozygous. Homozygotes were bred an additional two generations before arriving at The Jackson Laboratory.
Gene & Allele Details
| Allele Symbol | Tg(Cp-EGFP)25Gaia | ||
|---|---|---|---|
| Allele Name | transgene insertion 25, Nicholas R Gaiano | ||
| Common Name(s) | TNR; transgenic Notch reporter; | ||
| Mutation Made By | Nicholas Gaiano, Johns Hopkins Univ School of Medicine | ||
| Strain of Origin | (C57BL/6J x SJL/J)F2 | ||
| Site of Expression | Widespread expression during development and in the adult, including enriched hematopoietic stem cell (HSC) populations. As immature (double negative [DN]) thymocytes have differential expression patterns as they progress from DN1-DN4, these mice may also be useful in thymocyte maturation studies. | ||
| Expressed Gene | GFP, Green Fluorescent Protein, jellyfish | ||
| Green Fluorescent Protein (GFP), derived from the jellyfish Aequorea victoria, is a versatile reporter molecule which has found use in many biological applications. In some constructs the original molecule has been modified in order to enhance its fluorescence intensity (EGFP, enhanced GFP). When utilized in a transgenic construct, tissue expressing sufficient amounts of GFP will fluoresce when exposed to a 488 nm light source. | |||
| Promoter | C, C promoter (Cp) binding factor 1 (CBF1) binding site consensus sequence (derived from Epstein Barr Virus), | ||
| General Note | EGFP is expressed from this transgene when the promoter is activated by binding to the upstream CBF1 binding elements of a complex comprising CBF1, the transcriptional coactivator mastermind like 1 (Drosophila)(MML1), and the intracellular cleavage product resulting from interaction of the transmembrane Notch receptor with ligand. | ||
| Molecular Note | The transgene comprises four tandem copies of the C-promoter binding factor 1 (CBF1/RBPSUH) binding site of the Epstein-Barr virus major latency C promoter (Cp), followed by the SV40 minimal promoter and the coding sequence for enhanced green fluorescentprotein. Cells of mice bearing this transgene express green fluorescent protein when the Notch signaling pathway is activated. [MGI Ref ID J:96367] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Fluorescent Proteins (Generic GFP)
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, these mice are bred as hemizygotes or homozygotes. Donating investigator reports that homozygous females may have slightly reduced viability/health. |
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| Diet Information | LabDiet® 5K52/5K67 |
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View Fluorescent Protein Strains (145 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Animal Health Reports
Room Number AX11
Research Applications
This mouse can be used to support research in many areas including:
GFP relatedCell Biology Research
Signal Transduction
Developmental Biology Research
Internal/Organ Defects (hematopoietic defects)
Lymphoid Tissue Defects (hematopoietic defects)
Hematological Research
Hematopoietic Defects
Immunology and Inflammation Research
Lymphoid Tissue Defects (hematopoietic development)
Neurobiology Research
Fluorescent protein expression in neural tissue
Research Tools
Developmental Biology Research (transplantation marker for embryonic and adult tissue)
Fluorescent Proteins
Genetics Research (Tissue/Cell Markers: cell marker for bone marrow transplantation)
Genetics Research (Tissue/Cell Markers: multiple)
Immunology and Inflammation Research
Neurobiology Research (cell marker)
Research Tools
Fluorescent Proteins
References
Selected Reference(s)
Additional ReferencesDuncan AW; Rattis FM; DiMascio LN; Congdon KL; Pazianos G; Zhao C; Yoon K; Cook JM; Willert K; Gaiano N; Reya T. 2005. Integration of Notch and Wnt signaling in hematopoietic stem cell maintenance. Nat Immunol 6(3):314-22. [PubMed: 15665828] [MGI Ref ID J:96367]
Price and Supply Information
| Strain Name: | STOCK Tg(Cp-EGFP)25Gaia/J |
| Stock Number: | 005854 |
Price Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement. |
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| Supply Notes |
Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Effective September 26, 2007: License Requirements for Strains using Cre-lox Technology only apply in Canada, see Licenses for Strains using Cre-lox Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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