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Strain Name:

B6.Cg-Tg(Camk2a-Prkaca)426Tabe/J

Stock Number:

005855

Availability:

Repository-Cryopreserved


General Terms and Conditions

Genes & Alleles   Camk2a;   Prkaca;   Tg(Camk2a-Prkaca)426Tabe;


Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator Ted Abel,   University of Pennsylvania

Strain Description
Mice hemizygous for the transgene are viable and fertile with no gross anatomical abnormalities or structural lesions within the hippocampus or other regions. Transgene expression is observed in hippocampus (CA1, CA3, and dentate gyrus), neocortex, olfactory bulb, striatum, and amygdala. Transgenic mice have a 50% reduction in basal cAMP-dependent protein kinase A (PKA or PRKACA) activity, defective protein synthesis-dependent late phase of long-term potentiation, and reduced place cell stability in hippocampus. Mice have defective long-term, but not short-term, memory in hippocampus-based spatial and non-spatial tasks. This parallels the phenotype observed in mice treated with inhibitors of PKA or inhibitors of protein synthesis. Functional magnetic resonance imaging reveals reduced CA1 hippocampal signal. Transgenic mice are more sensitive to ethanol-induced sedation. Mice expressing this transgene may be useful in a wide variety of memory studies, including Alzheimer's disease, hippocampus-dependent long term memory, protein synthesis-dependent memory, amnesia, or age-related memory decline.

Strain Development
The cAMP-dependent protein kinase A (PKA or PRKACA) RIalpha regulatory subunit, R(AB), was mutated at both cAMP binding sites and placed under control of the mouse Camk2a promoter. The resulting construct was injected into the pronuclei of (B6CBAF1/J x B6CBAF1/J) zygotes. Chimeric males (founder line 426, R(AB)-2) were bred to C57BL/6J females. At some point, hemizygotes were bred to B6CBAF1/J to rescue the line. After the rescue, hemizygotes were bred to C57BL/6J for more than 10 generations before arrival at The Jackson Laboratory.

Gene & Allele Details

Allele Symbol Tg(Camk2a-Prkaca)426Tabe
Allele Name transgene insertion 426, Ted Abel
Common Name(s) CaMKII-R(AB); R(AB); R(AB)-2;
Mutation Made By Ted Abel,   University of Pennsylvania
Strain of Origin(C57BL/6J x CBA/J)F2
Expressed Gene Prkaca, protein kinase, cAMP dependent, catalytic, alpha, mouse, laboratory
Promoter Camk2a, calcium/calmodulin-dependent protein kinase II alpha, mouse, laboratory
Molecular Note The cAMP-dependent protein kinase A (PKA or PRKACA) RIalpha regulatory subunit, R(AB), was mutated at both cAMP binding sites and placed under control of the mouse Camk2a promoter. [MGI Ref ID J:38837]

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(Camk2a-Prkaca)426Tabe

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, hemizygotes are bred to wildtype siblings or to inbred C57BL/6J mice.

Related Strains

View Strains carrying other alleles of Camk2a     (10 strains)

Additional Web Information

Congenic Nomenclature
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.

Research Applications

This mouse can be used to support research in many areas including:

Neurobiology Research
Alzheimer's Disease
Behavioral and Learning Defects

References

Selected Reference(s)

Abel T; Nguyen PV; Barad M; Deuel TA; Kandel ER; Bourtchouladze R. 1997. Genetic demonstration of a role for PKA in the late phase of LTP and in hippocampus-based long-term memory. Cell 88(5):615-26. [PubMed: 9054501]  [MGI Ref ID J:38837]

Additional References

Price and Supply Information

Strain Name: B6.Cg-Tg(Camk2a-Prkaca)426Tabe/J
Stock Number: 005855

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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