Strain Name: |
B6.Cg-Tg(Camk2a-Prkaca)426Tabe/J |
|---|---|
Stock Number: |
005855 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Camk2a; Prkaca; Tg(Camk2a-Prkaca)426Tabe; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Ted Abel, University of Pennsylvania Strain Description
Mice hemizygous for the transgene are viable and fertile with no gross anatomical abnormalities or structural lesions within the hippocampus or other regions. Transgene expression is observed in hippocampus (CA1, CA3, and dentate gyrus), neocortex, olfactory bulb, striatum, and amygdala. Transgenic mice have a 50% reduction in basal cAMP-dependent protein kinase A (PKA or PRKACA) activity, defective protein synthesis-dependent late phase of long-term potentiation, and reduced place cell stability in hippocampus. Mice have defective long-term, but not short-term, memory in hippocampus-based spatial and non-spatial tasks. This parallels the phenotype observed in mice treated with inhibitors of PKA or inhibitors of protein synthesis. Functional magnetic resonance imaging reveals reduced CA1 hippocampal signal. Transgenic mice are more sensitive to ethanol-induced sedation. Mice expressing this transgene may be useful in a wide variety of memory studies, including Alzheimer's disease, hippocampus-dependent long term memory, protein synthesis-dependent memory, amnesia, or age-related memory decline.Strain Development
The cAMP-dependent protein kinase A (PKA or PRKACA) RIalpha regulatory subunit, R(AB), was mutated at both cAMP binding sites and placed under control of the mouse Camk2a promoter. The resulting construct was injected into the pronuclei of (B6CBAF1/J x B6CBAF1/J) zygotes. Chimeric males (founder line 426, R(AB)-2) were bred to C57BL/6J females. At some point, hemizygotes were bred to B6CBAF1/J to rescue the line. After the rescue, hemizygotes were bred to C57BL/6J for more than 10 generations before arrival at The Jackson Laboratory.
Gene & Allele Details
| Allele Symbol | Tg(Camk2a-Prkaca)426Tabe | ||
|---|---|---|---|
| Allele Name | transgene insertion 426, Ted Abel | ||
| Common Name(s) | CaMKII-R(AB); R(AB); R(AB)-2; | ||
| Mutation Made By | Ted Abel, University of Pennsylvania | ||
| Strain of Origin | (C57BL/6J x CBA/J)F2 | ||
| Expressed Gene | Prkaca, protein kinase, cAMP dependent, catalytic, alpha, mouse, laboratory | ||
| Promoter | Camk2a, calcium/calmodulin-dependent protein kinase II alpha, mouse, laboratory | ||
| Molecular Note | The cAMP-dependent protein kinase A (PKA or PRKACA) RIalpha regulatory subunit, R(AB), was mutated at both cAMP binding sites and placed under control of the mouse Camk2a promoter. [MGI Ref ID J:38837] | ||
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(Camk2a-Prkaca)426Tabe
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, hemizygotes are bred to wildtype siblings or to inbred C57BL/6J mice. |
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Related Strains
Strains carrying other alleles of Camk2a
002362 B6.129P2-Camk2atm1Sva/J 007574 B6.Cg-Tg(Camk2a-Crebbp*)1364Tabe/J 005359 B6.Cg-Tg(Camk2a-cre)T29-1Stl/J 007004 B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ 003010 B6;CBA-Tg(Camk2a-tTA)1Mmay/J 004995 C3H-Tg(Camk2a-Creb1/ESR1)3Sva/J 006575 C57BL/6-Camk2atm1Vyb/J 006579 C57BL/6-Tg(Camk2a-Bdnf)A9Stl/J 005087 C57BL/6-Tg(Camk2a-IDE)1Selk/J 005086 C57BL/6-Tg(Camk2a-MME)3Selk/J View Strains carrying other alleles of Camk2a (10 strains)
Additional Web Information
Congenic Nomenclature
Visit the Alzheimer's Disease Mouse Model Resource site for helpful information on Alzheimer's Disease and research resources.
Research Applications
This mouse can be used to support research in many areas including:
Neurobiology Research
Alzheimer's Disease
Behavioral and Learning Defects
References
Selected Reference(s)
Additional ReferencesAbel T; Nguyen PV; Barad M; Deuel TA; Kandel ER; Bourtchouladze R. 1997. Genetic demonstration of a role for PKA in the late phase of LTP and in hippocampus-based long-term memory. Cell 88(5):615-26. [PubMed: 9054501] [MGI Ref ID J:38837]
Price and Supply Information
| Strain Name: | B6.Cg-Tg(Camk2a-Prkaca)426Tabe/J |
| Stock Number: | 005855 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
General Terms and Conditions
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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