Strain Name:

C57BL/6J-Tg(ACTB-DDAH1)1Jpck/J

Stock Number:

005863

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Cryopreserved - Ready for recovery

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Common Names: DDAH transgenic mice;     hDDAH transgenic mice;    

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationN9p
Generation Definitions
 
Donating Investigator John P. Cooke,   Stanford University Medical Center

Description
Hemizygous mice are viable and fertile with no anatomic abnormalities. Transgene expression is observed in aorta, heart, and brain. Transgenic dimethylarginine dimethylaminohydrolase (DDAH) activity is reflected in a reduction of plasma asymmetric dimethylarginine (ADMA). Nitric oxide synthase (NOS) activity is significantly increased in transgenic skeletal and cardiac muscle. Transgenic mice have lower blood pressure and higher heart rate. Transgenic mice may be useful in studies of atherosclerosis, hypertension, hyperlipidemia, hypercholesterolemia, hyperhomocystinemia, diabetes mellitus, stroke, renal failure, preeclampsia, and other conditions associated with vascular pathophysiology and/or cardiovascular disease.

Development
A human DDAH-I cDNA and SV40 derived from a modified human agouti expression vector were placed under control of the human B-actin promoter. This construct was injected into fertilized C57BL/6J eggs. Embryos were transferred into the oviducts of pseudopregnant B6CBAF1 foster mothers. Transgenic males were bred to C57BL/6J approximately 8-10 generations before arrival at The Jackson Laboratory.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of ACTB     (15 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(ACTB-DDAH1)1Jpck/0

        involves: C57BL/6J
  • cardiovascular system phenotype
  • abnormal cardiovascular system physiology   (MGI Ref ID J:104238)
    • decreased cardiac stroke volume
      • stroke volume is reduced by ~10% in transgenic mice   (MGI Ref ID J:104238)
    • decreased systemic arterial blood pressure
      • mean arterial pressure is reduced by ~10% compared to controls   (MGI Ref ID J:104238)
      • decreased systemic arterial systolic blood pressure
        • pressure is lower (105.9 mm Hg) than in age- and weight-matched conscious controls (112.7 mm Hg)   (MGI Ref ID J:104238)
        • systolic pressure difference is greater (13 mm Hg) in anesthetized transgenics (76 mm Hg) compared to controls (89 mm Hg)   (MGI Ref ID J:104238)
    • decreased systemic vascular resistance
      • systemic vascular resistance is reduced by ~10% compared to controls   (MGI Ref ID J:104238)
    • increased heart rate
      • transgenic mice display ~10% increase in heart rate   (MGI Ref ID J:104238)
  • homeostasis/metabolism phenotype
  • abnormal enzyme/ coenzyme level
    • nitric oxide synthase (NOS) activity is significantly increased in transgenic skeletal muscle; cardiac tissue homogenates have higher NOS activity   (MGI Ref ID J:104238)
  • abnormal nitric oxide homeostasis
    • transgenic skeletal muscle homogenates have greater NOS activity   (MGI Ref ID J:104238)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cardiovascular Research
Heart Abnormalities
      tachycardia
Hypercholesterolemia
Hypertension
Hypotension

Diabetes and Obesity Research
Type 1 Diabetes (IDDM)

Internal/Organ Research
Heart Abnormalities

Metabolism Research
Lipid Metabolism

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(ACTB-DDAH1)1Jpck
Allele Name transgene insertion 1, John P Cooke
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) DDAH; DDAH1 TG;
Mutation Made By John Cooke,   Stanford University Medical Center
Strain of OriginC57BL/6J
Expressed Gene DDAH1, dimethylarginine dimethylaminohydrolase 1, human
Promoter ACTB, actin, beta, human
Molecular Note A human DDAH-I cDNA and SV40 derived from a modified human agouti expression vector were placed under control of the human B-actin promoter. [MGI Ref ID J:104238]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(ACTB-DDAH1)1Jpck, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Dayoub H; Achan V; Adimoolam S; Jacobi J; Stuehlinger MC; Wang BY; Tsao PS; Kimoto M; Vallance P; Patterson AJ; Cooke JP. 2003. Dimethylarginine dimethylaminohydrolase regulates nitric oxide synthesis: genetic and physiological evidence. Circulation 108(24):3042-7. [PubMed: 14638548]  [MGI Ref ID J:104238]

Additional References

Tg(ACTB-DDAH1)1Jpck related

Dayoub H; Rodionov RN; Lynch C; Cooke JP; Arning E; Bottiglieri T; Lentz SR; Faraci FM. 2008. Overexpression of dimethylarginine dimethylaminohydrolase inhibits asymmetric dimethylarginine-induced endothelial dysfunction in the cerebral circulation. Stroke 39(1):180-4. [PubMed: 18063827]  [MGI Ref ID J:148566]

Jacobi J; Maas R; Cardounel AJ; Arend M; Pope AJ; Cordasic N; Heusinger-Ribeiro J; Atzler D; Strobel J; Schwedhelm E; Boger RH; Hilgers KF. 2010. Dimethylarginine dimethylaminohydrolase overexpression ameliorates atherosclerosis in apolipoprotein E-deficient mice by lowering asymmetric dimethylarginine. Am J Pathol 176(5):2559-70. [PubMed: 20348244]  [MGI Ref ID J:160760]

Jacobi J; Maas R; Cordasic N; Koch K; Schmieder RE; Boger RH; Hilgers KF. 2008. Role of asymmetric dimethylarginine for angiotensin II-induced target organ damage in mice. Am J Physiol Heart Circ Physiol 294(2):H1058-66. [PubMed: 18156199]  [MGI Ref ID J:132364]

Jacobi J; Sydow K; von Degenfeld G; Zhang Y; Dayoub H; Wang B; Patterson AJ; Kimoto M; Blau HM; Cooke JP. 2005. Overexpression of dimethylarginine dimethylaminohydrolase reduces tissue asymmetric dimethylarginine levels and enhances angiogenesis. Circulation 111(11):1431-8. [PubMed: 15781754]  [MGI Ref ID J:108993]

Leypoldt F; Choe CU; Gelderblom M; von Leitner EC; Atzler D; Schwedhelm E; Gerloff C; Sydow K; Boger RH; Magnus T. 2009. Dimethylarginine dimethylaminohydrolase-1 transgenic mice are not protected from ischemic stroke. PLoS One 4(10):e7337. [PubMed: 19809508]  [MGI Ref ID J:154104]

Pullamsetti SS; Savai R; Dumitrascu R; Dahal BK; Wilhelm J; Konigshoff M; Zakrzewicz D; Ghofrani HA; Weissmann N; Eickelberg O; Guenther A; Leiper J; Seeger W; Grimminger F; Schermuly RT. 2011. The role of dimethylarginine dimethylaminohydrolase in idiopathic pulmonary fibrosis. Sci Transl Med 3(87):87ra53. [PubMed: 21677199]  [MGI Ref ID J:173535]

Rodionov RN; Dayoub H; Lynch CM; Wilson KM; Stevens JW; Murry DJ; Kimoto M; Arning E; Bottiglieri T; Cooke JP; Baumbach GL; Faraci FM; Lentz SR. 2010. Overexpression of dimethylarginine dimethylaminohydrolase protects against cerebral vascular effects of hyperhomocysteinemia. Circ Res 106(3):551-8. [PubMed: 20019334]  [MGI Ref ID J:170881]

Sydow K; Mondon CE; Schrader J; Konishi H; Cooke JP. 2008. Dimethylarginine dimethylaminohydrolase overexpression enhances insulin sensitivity. Arterioscler Thromb Vasc Biol 28(4):692-7. [PubMed: 18239148]  [MGI Ref ID J:148654]

Sydow K; Schmitz C; von Leitner EC; von Leitner R; Klinke A; Atzler D; Krebs C; Wieboldt H; Ehmke H; Schwedhelm E; Meinertz T; Blankenberg S; Boger RH; Magnus T; Baldus S; Wenzel U. 2012. Dimethylarginine dimethylaminohydrolase1 is an organ-specific mediator of end organ damage in a murine model of hypertension. PLoS One 7(10):e48150. [PubMed: 23110194]  [MGI Ref ID J:192303]

Tanaka M; Sydow K; Gunawan F; Jacobi J; Tsao PS; Robbins RC; Cooke JP. 2005. Dimethylarginine dimethylaminohydrolase overexpression suppresses graft coronary artery disease. Circulation 112(11):1549-56. [PubMed: 16144995]  [MGI Ref ID J:118229]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice are bred as hemizygotes. Homozygous mice were not propagated because litter size was small.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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